LOCUS       sequence-407945-        2749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 Position 1 pUC19 acceptor vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Victoria AJ, Selao TT, Moreno-Cabezuelo JA, Mills LA, Gale GAR, 
            Lea-Smith DJ, McCormick AJ
  TITLE     A toolbox to engineer the highly productive cyanobacterium 
            Synechococcus sp. PCC 11901.
  JOURNAL   Plant Physiol. 2024 May 7:kiae261. doi: 10.1093/plphys/kiae261.
  PUBMED    38713768
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2024 May 7:kiae261. doi: 10.1093/plphys/kiae261."
COMMENT     Sequence Label: pCA1.327
FEATURES             Location/Qualifiers
     source          1..2749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..71)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(55..71)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(68..90)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    79..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(103..109,110..127,128..133))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    148..169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(318..335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(489..1077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(569..588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1248..2039,2040..2108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1871..1890
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2109..2213)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2281..2299
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2337..2359)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(2485..2749,1..59))
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCNLSTICS
                     DAA"
     primer_bind     2490..2509
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2703..2725
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2717..2734
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2718..2734
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(2735..2749,1..42)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
ORIGIN
        1 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata
       61 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      121 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      181 ctcactgccc gctttccagt cggcaaacct gtcgtgccag ctgcggtctc actccgaaac
      241 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      301 gggcgctcat ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      481 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      661 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      901 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1081 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1141 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1201 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1261 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1321 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1381 ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1441 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1501 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1561 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1621 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     1681 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1741 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1801 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1861 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1921 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     1981 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2041 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2101 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2161 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2221 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2281 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     2341 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcagcgtg agaccgtcac
     2401 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     2461 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagattgca
     2521 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     2581 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     2641 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     2701 ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggt
//
