LOCUS       sequence-407950-        1155 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  in vitro MAPK-Sensor for luminescent detection of ERK and 
            p38-activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Poti AL, Denes L, Papp K, Bato C, Banoczi Z, Remenyi A, Alexa A
  TITLE     Phosphorylation-Assisted Luciferase Complementation Assay Designed 
            to Monitor Kinase Activity and Kinase-Domain-Mediated 
            Protein-Protein Binding.
  JOURNAL   Int J Mol Sci. 2023 Oct 3;24(19):14854. doi: 10.3390/ijms241914854.
  PUBMED    37834301
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2023-10-3"; volume: "24(19):14854. doi"; pages: " 
            10.3390/ijms241914854"
COMMENT     Sequence Label: MEF2A-Sensor
FEATURES             Location/Qualifiers
     source          1..1155
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(205..225)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(286..309)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     regulatory      417..426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagca gaatctcctc
       61 gaacagccgg tagccggtca cgcggccgct caccggggta cgaggtacgt cgacgctacc
      121 agagccgctg ctaccgccac tgctaccact gcccatatgg ctaggaggaa taacaacacg
      181 cagatcaggt ttgcggctag cggatccctg aaaataaaga ttctcgctca tggaaccgcc
      241 accaccaccg cagccactag tgttgttgtt cgagctcgaa ttagtctgcg cgtctttcag
      301 ggcttcatcg acagtctgac gaccgctggc ggcgttgatc accgcagtac gcacggcata
      361 ccagaaagcg gacatctgcg ggatgttcgg catgatttca cctttctggg cgttttccat
      421 ggtggcggca atacgtggat ctttcgccaa ctcttcctcg taagacttca gcgctacggc
      481 acccagcggt ttgtctttat taaccgcttc cagaccttca tcagtcagca gatagttttc
      541 gaggaactct tttgccagct ctttgttcgg actggcggcg ttaatacctg cgctcagcac
      601 gccaacgaac ggtttggatg gttgaccctt gaaggtcggc agtaccgtta caccataatt
      661 cactttgctg gtgtcgatgt tggaccatgc ccacgggccg ttgatggtca tcgctgtttc
      721 gcctttatta aaggcagctt ctgcgatgga gtaatcggtg tctgcattca tgtgtttgtt
      781 tttaatcagg tcaaccagga aggtcagacc cgctttcgcg ccagcgttat ccacgcccac
      841 gtctttaatg tcgtacttgc cgttttcata cttgaacgca taacccccgt cagcagcaat
      901 cagcggccag gtgaagtacg gttcttgcag gttgaacatc agcgcgctct tacctttcgc
      961 tttcagttct ttatccagcg ccgggatctc ttcccaggtt tttggcgggt tcggcagcag
     1021 atctttgtta taaatcagcg ataacgcttc aacagcgatc gggtaagcaa tcagcttgcc
     1081 gttgtaacgt acggcatccc aggtaaacgg atacagcttg tcctggaacg ctttgtccgg
     1141 ggtgatttca gccaa
//
