LOCUS       sequence-407956-        2187 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of Hm4Di fused to YFP; Chemogenetics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Sanford CA, Soden ME, Baird MA, Miller SM, Schulkin J, Palmiter RD, 
            Clark M, Zweifel LS
  TITLE     A Central Amygdala CRF Circuit Facilitates Learning about Weak 
            Threats.
  JOURNAL   Neuron. 2017 Jan 4;93(1):164-178. doi: 10.1016/j.neuron.2016.11.034.
            Epub 2016 Dec 22.
  PUBMED    28017470
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2016.11.034"; journalName: "Neuron"; date: 
            "2017-01-4- 4"; volume: "93"; issue: "1"; pages: "164-178"
COMMENT     Sequence Label: pAAV-FLEX-HM4Di-YFP
FEATURES             Location/Qualifiers
     source          1..2187
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1462..1471
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1468..1470,1471..1473,1474..2187)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1513..1534)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1774..1793)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2121..2142
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgctagcac cggctagcat ggccaacttc acacctgtca atggcagctc gggcaatcag
       61 tccgtgcgcc tggtcacgtc atcatcccac aatcgctatg agacggtgga aatggtcttc
      121 attgccacag tgacaggctc cctgagcctg gtgactgtcg tgggcaacat cctggtgatg
      181 ctgtccatca aggtcaacag gcagctgcag acagtcaaca actacttcct cttcagcctg
      241 gcgtgtgctg atctcatcat aggcgccttc tccatgaacc tctacaccgt gtacatcatc
      301 aagggctact ggcccctggg cgccgtggtc tgcgacctgt ggctggccct ggactgcgtg
      361 gtgagcaacg cctccgtcat gaaccttctc atcatcagct ttgaccgcta cttctgcgtc
      421 accaagcctc tcacctaccc tgcccggcgc accaccaaga tggcaggcct catgattgct
      481 gctgcctggg tactgtcctt cgtgctctgg gcgcctgcca tcttgttctg gcagtttgtg
      541 gtgggtaagc ggacggtgcc cgacaaccag tgcttcatcc agttcctgtc caacccagca
      601 gtgacctttg gcacagccat tgctggcttc tacctgcctg tggtcatcat gacggtgctg
      661 tacatccaca tctccctggc cagtcgcagc cgagtccaca agcaccggcc cgagggcccg
      721 aaggagaaga aagccaagac gctggccttc ctcaagagcc cactaatgaa gcagagcgtc
      781 aagaagcccc cgcccgggga ggccgcccgg gaggagctgc gcaatggcaa gctggaggag
      841 gcccccccgc cagcgctgcc accgccaccg cgccccgtgg ctgataagga cacttccaat
      901 gagtccagct caggcagtgc cacccagaac accaaggaac gcccagccac agagctgtcc
      961 accacagagg ccaccacgcc cgccatgccc gcccctcccc tgcagccgcg ggccctcaac
     1021 ccagcctcca gatggtccaa gatccagatt gtgacgaagc agacaggcaa tgagtgtgtg
     1081 acagccattg agattgtgcc tgccacgccg gctggcatgc gccctgcggc caacgtggcc
     1141 cgcaagttcg ccagcatcgc tcgcaaccag gtgcgcaaga agcggcagat ggcggcccgg
     1201 gagcgcaaag tgacacgaac gatctttgcc attctactag ccttcatcct cacctggacg
     1261 ccctacaacg tcatggtcct ggtgaacacc ttctgccaga gctgcatccc tgacacggtg
     1321 tggtccattg gctactggct ctgctacgtc aacagcacca tcaaccctgc ctgctatgct
     1381 ctgtgcaacg ccacctttaa aaagaccttc cggcacctgc tgctgtgcca gtatcggaac
     1441 atcggcactg ccagggcggc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     1501 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1561 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1621 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc
     1681 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1741 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1801 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1861 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1921 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1981 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2041 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
     2101 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2161 ctcggcatgg acgagctgta caagtaa
//
