LOCUS       sequence-407981-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of mCherry-tagged Chrimson; Optogenetics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Soden ME, Chung AS, Cuevas B, Resnick JM, Awatramani R, Zweifel LS
  TITLE     Anatomic resolution of neurotransmitter-specific projections to the 
            VTA reveals diversity of GABAergic inputs.
  JOURNAL   Nat Neurosci. 2020 Aug;23(8):968-980. doi: 
            10.1038/s41593-020-0657-z. Epub 2020 Jun 15.
  PUBMED    32541962
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-020-0657-z"; journalName: "Nat Neurosci"; date: 
            "2020-08"; volume: "23"; issue: "8"; pages: "968-980"
COMMENT     Sequence Label: pAAV-CAG-FLEX-Chrimson-TdTomato
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1050
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=ChrimsonR
                     /note="K176R mutant with accelerated closing kinetics"
                     /translation="MAELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTE
                     WWCDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSI
                     AIALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRY
                     FEWLLSCPVILIRLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIV
                     SCIYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLK
                     LSPYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEE
                     FVEEEDEDTV"
ORIGIN
        1 atggctgagc tgatcagcag cgccaccaga tctctgtttg ccgccggagg catcaaccct
       61 tggcctaacc cctaccacca cgaggacatg ggctgtggag gaatgacacc tacaggcgag
      121 tgcttcagca ccgagtggtg gtgtgaccct tcttacggac tgagcgacgc cggatacgga
      181 tattgcttcg tggaggccac aggcggctac ctggtcgtgg gagtggagaa gaagcaggct
      241 tggctgcaca gcagaggcac accaggagaa aagatcggcg cccaggtctg ccagtggatt
      301 gctttcagca tcgccatcgc cctgctgaca ttctacggct tcagcgcctg gaaggccact
      361 tgcggttggg aggaggtcta cgtctgttgc gtcgaggtgc tgttcgtgac cctggagatc
      421 ttcaaggagt tcagcagccc cgccacagtg tacctgtcta ccggcaacca cgcctattgc
      481 ctgcgctact tcgagtggct gctgtcttgc cccgtgatcc tgatcagact gagcaacctg
      541 agcggcctga agaacgacta cagcaagcgg accatgggcc tgatcgtgtc ttgcgtggga
      601 atgatcgtgt tcggcatggc cgcaggactg gctaccgatt ggctcaagtg gctgctgtat
      661 atcgtgtctt gcatctacgg cggctacatg tacttccagg ccgccaagtg ctacgtggaa
      721 gccaaccaca gcgtgcctaa aggccattgc cgcatggtcg tgaagctgat ggcctacgct
      781 tacttcgcct cttggggcag ctacccaatc ctctgggcag tgggaccaga aggactgctg
      841 aagctgagcc cttacgccaa cagcatcggc cacagcatct gcgacatcat cgccaaggag
      901 ttttggacct tcctggccca ccacctgagg atcaagatcc acgagcacat cctgatccac
      961 ggcgacatcc ggaagaccac caagatggag atcggaggcg aggaggtgga agtggaagag
     1021 ttcgtggagg aggaggacga ggacacagtg
//
