LOCUS       sequence-407998-        9165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad host-range bacterial expression vector with constitutive PsbA 
            promoter; mNeonGreen (codon optimized for P. fluorescens).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Wilton R, Ahrendt AJ, Shinde S, Sholto-Douglas DJ, Johnson JL, 
            Brennan MB, Kemner KM
  TITLE     A New Suite of Plasmid Vectors for Fluorescence-Based Imaging of 
            Root Colonizing Pseudomonads.
  JOURNAL   Front Plant Sci. 2018 Feb 1;8:2242. doi: 10.3389/fpls.2017.02242. 
            eCollection 2017.
  PUBMED    29449848
REFERENCE   2  (bases 1 to 9165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2018-02-1"; volume: "8:2242. doi"; pages: " 
            10.3389/fpls.2017.02242. eCollection 2017"
COMMENT     Sequence Label: pSW003-PpsbA-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..9165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(44..1243)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             1349..1999
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     RBS             2954..2976
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3704..3721
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4208..4225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4342..4887)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4422..4441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      5596..5790
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5856..6929)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7358..7987)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 atagttctag ctagtactag agcggcccct atcgtttcca cgatcagcga tcggctcgtt
       61 gccctgcgcc gctccaaagc ccgcgacgca gcgccggcag gcagagcaag tagagggcag
      121 cgcctgcaat ccatgcccac ccgttccacg ttgttataga agccgcatag atcgccgtga
      181 agaggagggg tccgacgatc gaggtcaggc tggtgagcgc cgccagtgag ccttgcagct
      241 gcccctgacg ttcctcatcc acctgcctgg acaacattgc ttgcagcgcc ggcattccga
      301 tgccacccga agcaagcagg accatgatcg ggaacgccat ccatccccgt gtcgcgaagg
      361 caagcaggat gtagcctgtg ccgtcggcaa tcattccgag catgagtgcc cgcctttcgc
      421 cgagccgggc ggctacaggg ccggtgatca ttgcctgggc gagtgaatgc agaatgccaa
      481 atgcggcaag cgaaatgccg atcgtggtcg cgtcccagtg aaagcgatcc tcgccgaaaa
      541 tgacccaaag cgcggccggc acctgtccga caagttgcat gatgaagaag accgccatca
      601 gggcggcgac gacggtcatg ccccgggccc accggaacga agcgagcggg ttgagagcct
      661 cccggcgtaa cggccggcgt tcgcctttgt gcgactccgg caaaaggaaa cagcccgtca
      721 ggaaattgag gccgttcaag gctgccgcgg cgaagaacgg agcgtggggg gagaaaccgc
      781 ccatcagccc accgagcaca ggtcccgcga ccatcccgaa cccgaaacag gcgctcatga
      841 agccgaagtg ccgcgcgcgc tcatcgccat cagtgatatc ggcaatataa gcgccggcta
      901 ccgccccagt cgccccggtg atgccggcca cgatccgccc gatatagaga acccaaagga
      961 aaggcgctgt cgccatgatg gcgtagtcga cagtggcgcc ggccagcgag acgagcaaga
     1021 ttggccgccg cccgaaacga tccgacagcg cgcccagcac aggtgcgcag gcaaattgca
     1081 ccaacgcata cagcgccagc agaatgccat agtgggcggt gacgtcgttc gagtgaacca
     1141 gatcgcgcag gaggcccggc agcaccggca taatcaggcc gatgccgaca gcgtcgagcg
     1201 cgacagtgct cagaattacg atcaggggta tgttgggttt cacgtctggc ctccggacca
     1261 gcctccgctg gtccgattga acgcgcggat tctttatcac tgataagttg gtggacatat
     1321 tatgtttatc agtgataaag tgtcaagcat gacaaagttg cagccgaata cagtgatccg
     1381 tgccgccctg gacctgttga acgaggtcgg cgtagacggt ctgacgacac gcaaactggc
     1441 ggaacggttg ggggttcagc agccggcgct ttactggcac ttcaggaaca agcgggcgct
     1501 gctcgacgca ctggccgaag ccatgctggc ggagaatcat acgcattcgg tgccgagagc
     1561 cgacgacgac tggcgctcat ttctgatcgg gaatgcccgc agcttcaggc aggcgctgct
     1621 cgcctaccgc gatggcgcgc gcatccatgc cggcacgcga ccgggcgcac cgcagatgga
     1681 aacggccgac gcgcagcttc gcttcctctg cgaggcgggt ttttcggccg gggacgccgt
     1741 caatgcgctg atgacaatca gctacttcac tgttggggcc gtgcttgagg agcaggccgg
     1801 cgacagcgat gccggcgagc gcggcggcac cgttgaacag gctccgctct cgccgctgtt
     1861 gcgggccgcg atagacgcct tcgacgaagc cggtccggac gcagcgttcg agcagggact
     1921 cgcggtgatt gtcgatggat tggcgaaaag gaggctcgtt gtcaggaacg ttgaaggacc
     1981 gagaaagggt gacgattgat caggaccgct gccggagcgc aacccactca ctacagcaga
     2041 gccatgtaga caacatcccc tccccctttc caccgcgtca gacgcccgta gcagcccgct
     2101 acgggctttt tcatgccctg ccctagcgtc caagcctcac ggccgcgctc ggcctctctg
     2161 gcggccttct ggcgctgagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc
     2221 tgaatcgccc catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg
     2281 taggtggacc agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg
     2341 ggaagatgcg tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccgc
     2401 cgtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt
     2461 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     2521 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     2581 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     2641 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     2701 aatccggtga gaatggcaaa agctggccgc cacggcgata tcggatccat atgacgtcga
     2761 cgcgtggatc tcaatgaata ttggttgaca cgggcgtata agacatgtta tactgttgaa
     2821 taacaagcta tcaattctct attttgagaa gaattaatgt gcttgggagt ccctgatgat
     2881 taaattaaat aaaccaagat tttaccatga ctgcaatttt agagagacgg gggatcgatc
     2941 ctcttgaaat aattttgttt aactttaaga aggagatata catatgaatt cgaaaggcga
     3001 agaagataac atggccagtt taccggcaac tcatgaatta catatttttg gaagtattaa
     3061 cggcgttgac tttgatatgg ttggacaggg caccggcaac ccgaacgatg gttatgaaga
     3121 attaaacctg aaatctacaa aaggcgacct gcaattctct ccctggattc ttgttccaca
     3181 cattggttat ggcttccacc aatacttacc ctatcctgat ggcatgtccc ctttccaagc
     3241 cgcgatggtg gatggctctg gttaccaagt acaccgtacc atgcaattcg aagatggagc
     3301 atcactcaca gtaaactacc gctatacata tgaaggttcc cacattaaag gcgaagcaca
     3361 agttaaaggt actggattcc ccgctgatgg accagttatg actaactcat tgaccgcagc
     3421 agactggtgt cgctcaaaaa aaacctatcc gaacgataaa acgattatta gcacatttaa
     3481 atggagttat acgaccggaa atggcaaacg ttaccgcagc acagcacgca caacctatac
     3541 atttgcaaaa ccaatggccg cgaattattt gaaaaaccaa ccgatgtatg tctttcgtaa
     3601 aacagaatta aaacacagca aaacagaatt aaattttaaa gaatggcaaa aagcatttac
     3661 cgacgttatg ggaatggatg aattatataa ataagcggcc gcacatcatc atcaccatca
     3721 ctaagcttta agcatctcga ggcctcggac tagcggatca gtgagggttt gcaactgcgg
     3781 gtcaaggatc tggatttcga tcacggcacg atcatcgtgc gggagggcaa gggctccaag
     3841 gatcgggcct tgatgttacc cgagagcttg gcacccagcc tgcgcgagca ggggaattga
     3901 tccggtggat gaccttttga atgaccttta atagattata ttactaatta attggggacc
     3961 ctagaggtcc ccttttttat tttaaaaatt ttttcacaaa acggtttaca agcataaagc
     4021 tcctcggact acctggtatg agtcagcaac accttcttca cgaggcagac ctcagcgcta
     4081 gcggagtgta tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat
     4141 gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt
     4201 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg
     4261 cttacgaacg gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga
     4321 gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat
     4381 ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4441 ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc
     4501 gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc
     4561 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg
     4621 gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca
     4681 ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa
     4741 aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta
     4801 gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag
     4861 attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaaggggtc tgacgctcag
     4921 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4981 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5041 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5101 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5161 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5221 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5281 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5341 agtttgcgca acgttgttgc cattgcctgg tatgagtcag caacaccttc ttcacgaggc
     5401 agacctcagc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg
     5461 cctggccgta ggccagccat ttttgagcgg ccatcggccg cgataggccg acgcgaagcg
     5521 gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg
     5581 cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa
     5641 gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac
     5701 cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc
     5761 tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct
     5821 gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc
     5881 tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc
     5941 aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc
     6001 ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct
     6061 gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc
     6121 aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg
     6181 tacatccaat caactagctc gatctcgatg tactccggcc gcccggtttc gctctttacg
     6241 atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg
     6301 gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc
     6361 aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg
     6421 tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat
     6481 tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg
     6541 gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca
     6601 gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg
     6661 cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc
     6721 ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga
     6781 tcagcggctg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg
     6841 gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt
     6901 accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc
     6961 cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac
     7021 acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt
     7081 tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg
     7141 atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc
     7201 ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc
     7261 aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg
     7321 tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt
     7381 cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg
     7441 ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa
     7501 tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga
     7561 tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct
     7621 taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa
     7681 tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc
     7741 cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc
     7801 ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac
     7861 tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg
     7921 ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa
     7981 ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac
     8041 cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt
     8101 ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag
     8161 gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc
     8221 gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg
     8281 cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ctgccagggc gtcggcctcg
     8341 gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg
     8401 ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct
     8461 ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg
     8521 agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg
     8581 gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc
     8641 atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg
     8701 ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg
     8761 tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc
     8821 tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc
     8881 gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag
     8941 cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa
     9001 gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg
     9061 gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact
     9121 gaacgtcaga agccgactgc actatagcag cggaggggtt ggatc
//
