LOCUS       sequence-407999-        9268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad host-range bacterial expression vector with constitutive Pc 
            promoter. Provides E. coli TorT signal peptide in frame with 
            mNeonGreen (codon optimized for expression in P. fluorescens); for 
            targeting mNeonGreen to the periplasm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9268)
  AUTHORS   Wilton R, Ahrendt AJ, Shinde S, Sholto-Douglas DJ, Johnson JL, 
            Brennan MB, Kemner KM
  TITLE     A New Suite of Plasmid Vectors for Fluorescence-Based Imaging of 
            Root Colonizing Pseudomonads.
  JOURNAL   Front Plant Sci. 2018 Feb 1;8:2242. doi: 10.3389/fpls.2017.02242. 
            eCollection 2017.
  PUBMED    29449848
REFERENCE   2  (bases 1 to 9268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2018-02-1"; volume: "8:2242. doi"; pages: " 
            10.3389/fpls.2017.02242. eCollection 2017"
COMMENT     Sequence Label: pSW002-Pc-TorT(sp)-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..9268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(44..1243)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             1349..1999
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     RBS             3003..3025
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3807..3824
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4311..4328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4445..4990)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4525..4544)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      5699..5893
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5959..7032)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7461..8090)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 atagttctag ctagtactag agcggcccct atcgtttcca cgatcagcga tcggctcgtt
       61 gccctgcgcc gctccaaagc ccgcgacgca gcgccggcag gcagagcaag tagagggcag
      121 cgcctgcaat ccatgcccac ccgttccacg ttgttataga agccgcatag atcgccgtga
      181 agaggagggg tccgacgatc gaggtcaggc tggtgagcgc cgccagtgag ccttgcagct
      241 gcccctgacg ttcctcatcc acctgcctgg acaacattgc ttgcagcgcc ggcattccga
      301 tgccacccga agcaagcagg accatgatcg ggaacgccat ccatccccgt gtcgcgaagg
      361 caagcaggat gtagcctgtg ccgtcggcaa tcattccgag catgagtgcc cgcctttcgc
      421 cgagccgggc ggctacaggg ccggtgatca ttgcctgggc gagtgaatgc agaatgccaa
      481 atgcggcaag cgaaatgccg atcgtggtcg cgtcccagtg aaagcgatcc tcgccgaaaa
      541 tgacccaaag cgcggccggc acctgtccga caagttgcat gatgaagaag accgccatca
      601 gggcggcgac gacggtcatg ccccgggccc accggaacga agcgagcggg ttgagagcct
      661 cccggcgtaa cggccggcgt tcgcctttgt gcgactccgg caaaaggaaa cagcccgtca
      721 ggaaattgag gccgttcaag gctgccgcgg cgaagaacgg agcgtggggg gagaaaccgc
      781 ccatcagccc accgagcaca ggtcccgcga ccatcccgaa cccgaaacag gcgctcatga
      841 agccgaagtg ccgcgcgcgc tcatcgccat cagtgatatc ggcaatataa gcgccggcta
      901 ccgccccagt cgccccggtg atgccggcca cgatccgccc gatatagaga acccaaagga
      961 aaggcgctgt cgccatgatg gcgtagtcga cagtggcgcc ggccagcgag acgagcaaga
     1021 ttggccgccg cccgaaacga tccgacagcg cgcccagcac aggtgcgcag gcaaattgca
     1081 ccaacgcata cagcgccagc agaatgccat agtgggcggt gacgtcgttc gagtgaacca
     1141 gatcgcgcag gaggcccggc agcaccggca taatcaggcc gatgccgaca gcgtcgagcg
     1201 cgacagtgct cagaattacg atcaggggta tgttgggttt cacgtctggc ctccggacca
     1261 gcctccgctg gtccgattga acgcgcggat tctttatcac tgataagttg gtggacatat
     1321 tatgtttatc agtgataaag tgtcaagcat gacaaagttg cagccgaata cagtgatccg
     1381 tgccgccctg gacctgttga acgaggtcgg cgtagacggt ctgacgacac gcaaactggc
     1441 ggaacggttg ggggttcagc agccggcgct ttactggcac ttcaggaaca agcgggcgct
     1501 gctcgacgca ctggccgaag ccatgctggc ggagaatcat acgcattcgg tgccgagagc
     1561 cgacgacgac tggcgctcat ttctgatcgg gaatgcccgc agcttcaggc aggcgctgct
     1621 cgcctaccgc gatggcgcgc gcatccatgc cggcacgcga ccgggcgcac cgcagatgga
     1681 aacggccgac gcgcagcttc gcttcctctg cgaggcgggt ttttcggccg gggacgccgt
     1741 caatgcgctg atgacaatca gctacttcac tgttggggcc gtgcttgagg agcaggccgg
     1801 cgacagcgat gccggcgagc gcggcggcac cgttgaacag gctccgctct cgccgctgtt
     1861 gcgggccgcg atagacgcct tcgacgaagc cggtccggac gcagcgttcg agcagggact
     1921 cgcggtgatt gtcgatggat tggcgaaaag gaggctcgtt gtcaggaacg ttgaaggacc
     1981 gagaaagggt gacgattgat caggaccgct gccggagcgc aacccactca ctacagcaga
     2041 gccatgtaga caacatcccc tccccctttc caccgcgtca gacgcccgta gcagcccgct
     2101 acgggctttt tcatgccctg ccctagcgtc caagcctcac ggccgcgctc ggcctctctg
     2161 gcggccttct ggcgctgagg tctgcctcgt gaagaaggtg ttgctgactc ataccaggcc
     2221 tgaatcgccc catcatccag ccagaaagtg agggagccac ggttgatgag agctttgttg
     2281 taggtggacc agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg
     2341 ggaagatgcg tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccgc
     2401 cgtcccgtca agtcagcgta atgctctgcc agtgttacaa ccaattaacc aattctgatt
     2461 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     2521 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     2581 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     2641 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     2701 aatccggtga gaatggcaaa agctggccgc cacggcgata tcggatccat atgacgtcga
     2761 cgcgttgccg atacaagaac aacagcgcgt tgagcgcctg ccggtgggtg gccggcgcca
     2821 cttgcttctc ggtggcgagc atggtcagaa aaccctcgac ttcagcttgc cccatttcgc
     2881 gcggatgtcg aaacccacca aggctgcggg ccgtccacaa cacaaatgcc ttggcccagt
     2941 agacataagc cttctcggtc tgtaggctgt aatgcaggta gcgaacccgt tcaagaaata
     3001 attttgttta actttaagaa ggagatatac atatgcgcgt actgctattt ttacttcttt
     3061 cccttttcat gttgccggca ttttcggcga attcgaaagg cgaagaagat aacatggcca
     3121 gtttaccggc aactcatgaa ttacatattt ttggaagtat taacggcgtt gactttgata
     3181 tggttggaca gggcaccggc aacccgaacg atggttatga agaattaaac ctgaaatcta
     3241 caaaaggcga cctgcaattc tctccctgga ttcttgttcc acacattggt tatggcttcc
     3301 accaatactt accctatcct gatggcatgt cccctttcca agccgcgatg gtggatggct
     3361 ctggttacca agtacaccgt accatgcaat tcgaagatgg agcatcactc acagtaaact
     3421 accgctatac atatgaaggt tcccacatta aaggcgaagc acaagttaaa ggtactggat
     3481 tccccgctga tggaccagtt atgactaact cattgaccgc agcagactgg tgtcgctcaa
     3541 aaaaaaccta tccgaacgat aaaacgatta ttagcacatt taaatggagt tatacgaccg
     3601 gaaatggcaa acgttaccgc agcacagcac gcacaaccta tacatttgca aaaccaatgg
     3661 ccgcgaatta tttgaaaaac caaccgatgt atgtctttcg taaaacagaa ttaaaacaca
     3721 gcaaaacaga attaaatttt aaagaatggc aaaaagcatt taccgacgtt atgggaatgg
     3781 atgaattata taaataagcg gccgcacatc atcatcacca tcactaagct ttaagcatct
     3841 cgaggcctcg gactagcgga tcagtgaggg tttgcaactg cgggtcaagg atctggattt
     3901 cgatcacggc acgatcatcg tgcgggaggg caagggctcc aaggatcggg ccttgatgtt
     3961 acccgagagc ttggcaccca gcctgcgcga gcaggggaat tgatccggtg gatgaccttt
     4021 tgaatgacct ttaatagatt atattactaa ttaattgggg accctagagg tccccttttt
     4081 tattttaaaa attttttcac aaaacggttt acaagcataa agctcctcgg actacctggt
     4141 atgagtcagc aacaccttct tcacgaggca gacctcagcg ctagcggagt gtatactggc
     4201 ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag
     4261 gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact
     4321 gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga
     4381 gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc
     4441 cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt
     4501 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg
     4561 tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt
     4621 tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt
     4681 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4741 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
     4801 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
     4861 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
     4921 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
     4981 acgatctcaa gaagatcatc ttattaaggg gtctgacgct cagtggaacg aaaactcacg
     5041 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5101 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5161 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5221 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5281 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5341 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5401 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5461 tgccattgcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgccggcg
     5521 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     5581 catttttgag cggccatcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     5641 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     5701 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     5761 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     5821 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     5881 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     5941 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     6001 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     6061 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     6121 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     6181 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     6241 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     6301 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     6361 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     6421 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     6481 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     6541 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     6601 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     6661 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     6721 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     6781 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     6841 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ctgcttgcca
     6901 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     6961 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     7021 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     7081 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     7141 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     7201 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     7261 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     7321 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     7381 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     7441 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     7501 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     7561 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     7621 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     7681 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     7741 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     7801 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     7861 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     7921 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     7981 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     8041 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     8101 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     8161 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     8221 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     8281 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     8341 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     8401 ctcttggcgt tcattctcgg cggctgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     8461 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     8521 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     8581 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     8641 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     8701 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     8761 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     8821 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     8881 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     8941 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     9001 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     9061 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     9121 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     9181 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     9241 tgcactatag cagcggaggg gttggatc
//
