LOCUS       sequence-408010-        8060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector for expression of human MAVS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Dixit E, Boulant S, Zhang Y, Lee AS, Odendall C, Shum B, Hacohen N, 
            Chen ZJ, Whelan SP, Fransen M, Nibert ML, Superti-Furga G, Kagan JC
  TITLE     Peroxisomes are signaling platforms for antiviral innate immunity.
  JOURNAL   Cell. 2010 May 14;141(4):668-81. Epub 2010 May 6.
  PUBMED    20451243
REFERENCE   2  (bases 1 to 8060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2010-05-14"; volume: "141(4)"; pages: "668-81. Epub 2010 May 
            6"
COMMENT     Sequence Label: MAVS-WT
FEATURES             Location/Qualifiers
     source          1..8060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(155..946,947..1015))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     778..797
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1016..1120)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1188..1206
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1244..1266)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1366..1385
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1579..1601
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1729..1748
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             1948..2464
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    2528..2869
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             2936..3352
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3282..3304
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3320..3336
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5041..5607
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5057..5609
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5201..5218)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5428..5447
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5621..5623,5624..5626,5627..6340)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5666..5687)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5927..5946)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6274..6295
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6402..6916
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    7078..7094
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7102..7108,7109..7126,7127..7132))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7147..7168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7285..7302)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7456..8044)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7536..7555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
       61 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      121 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      181 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      241 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
      301 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      361 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      421 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      481 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      541 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
      601 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
      661 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
      721 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
      781 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
      841 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
      901 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
      961 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1021 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1081 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1141 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     1201 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     1261 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     1321 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     1381 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     1441 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     1501 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     1561 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggcgcaa ggaatggtgc
     1621 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
     1681 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
     1741 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
     1801 gtagaggcga ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt
     1861 gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat
     1921 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     1981 agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat agagaagttc
     2041 agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata tctgtggtaa
     2101 gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg tcccgccctc
     2161 agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     2221 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     2281 cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgatagact
     2341 gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg
     2401 actcgctgat ccttgggagg gtctcctcag attgattgac tgcccacctc gggggtcttt
     2461 catttggagg ttccaccgag atttggagac ccctgcccag ggaccaccga cccccccgcc
     2521 gggaggtaag ctggccagcg gtcgtttcgt gtctgtctct gtctttgtgc gtgtttgtgc
     2581 cggcatctaa tgtttgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg
     2641 gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
     2701 ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
     2761 cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
     2821 tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
     2881 catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
     2941 gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
     3001 tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
     3061 gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
     3121 ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
     3181 cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
     3241 taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
     3301 gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattag
     3361 atctctcgag atgccgtttg ctgaagacaa gacctataag tatatctgcc gcaatttcag
     3421 caatttttgc aatgtggatg ttgtagagat tctgccttac ctgccctgcc tcacagcaag
     3481 agaccaggat cgactgcggg ccacctgcac actctcaggg aaccgggaca ccctctggca
     3541 tctcttcaat acccttcagc ggcggcccgg ctgggtggag tacttcattg cggcactgag
     3601 gggctgtgag ctagttgatc tcgcggacga agtggcctct gtctacgaga gctaccagcc
     3661 tcggacctcg gaccgtcccc cagacccact ggagccaccg tcacttcctg ctgagaggcc
     3721 agggcccccc acacctgctg cggcccacag catcccctac aacagctgca gagagaagga
     3781 gccaagttac cccatgcctg tccaggagac ccaggcgcca gagtccccag gagagaattc
     3841 agagcaagcc ctgcagacgc tcagccccag agccatccca aggaatccag atggtggccc
     3901 cctggagtcc tcctctgacc tggcagccct cagccctctg acctccagcg ggcatcagga
     3961 gaaggacaca gaactgggca gtacccacac agcaggtgcg acctccagcc tcacaccatc
     4021 ccgtgggcct gtgtctccat ctgtctcctt ccagcccctg gcccgttcca cccccagggc
     4081 aagccgcttg cctggaccca cagggtcagt tgtatctact ggcacctcct tctcctcctc
     4141 atcccctggc ttggcctctg caggggctgc agagggtaaa cagggtgcag agagtgacca
     4201 ggccgagcct atcatctgct ccagtggggc agaggcacct gccaactctc tgccctccaa
     4261 agtgcctacc accttgatgc ctgtgaacac agtggccctg aaagtgcctg ccaacccagc
     4321 atctgtcagc acagtgccct ccaagttgcc aactagctca aagccccctg gtgcagtgcc
     4381 ttctaatgcg ctcaccaatc cagcaccatc caaattgccc atcaactcaa cccgtgctgg
     4441 catggtgcca tccaaagtgc ctactagcat ggtgctcacc aaggtgtctg ccagcacagt
     4501 ccccactgac gggagcagca gaaatgagga gaccccagca gctccaacac ccgccggcgc
     4561 cactggaggc agctcagcct ggctagacag cagctttgag aataggggcc ttgggtcgga
     4621 gctgagtaag cctggcgtgc tggcatccca ggtagacagc ccgttctcgg gctgcttcga
     4681 ggatcttgcc atcagtgcca gcacctcctt gggcatgggg ccctgccatg gcccagagga
     4741 gaatgagtat aagtccgagg gcacctttgg gatccacgtg gctgagaacc ccagcatcca
     4801 gctcctggag ggcaaccctg ggccacctgc ggacccggat ggcggcccca ggccacaagc
     4861 cgaccggaag ttccaggaga gggaggtgcc atgccacagg ccctcacctg gggctctgtg
     4921 gctccaggtg gctgtgacag gggtgctggt agtcacactc ctggtggtgc tgtaccggcg
     4981 gcgtctgcac taggcggccg cgtttaaacg tcgacggtat cgataagctt gatatcgaat
     5041 tccgcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     5101 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     5161 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     5221 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     5281 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     5341 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     5401 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     5461 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     5521 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     5581 gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca agggcgagga
     5641 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     5701 gttcagcgtg tctggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     5761 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     5821 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5881 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     5941 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     6001 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     6061 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg cgaacttcaa
     6121 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     6181 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     6241 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     6301 cgccgggatc actctcggca tggacgagct gtacaagtaa tgaattaatt aagaattcga
     6361 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     6421 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag
     6481 aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg
     6541 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     6601 cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     6661 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     6721 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt
     6781 cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     6841 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     6901 agcgggggtc tttcatgggt aacagtttct tgaagttgga gaacaacatt ctgagggtag
     6961 gagtcgaata ttaagtaatc ctgactcaat tagccactgt tttgaatcca catactccaa
     7021 tactcctgaa atagttcatt atggacagcg cagaaagagc tggggagaat tgtgaaattg
     7081 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7141 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7201 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7261 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7321 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7381 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7441 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7861 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7921 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7981 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8041 tcaagaagat cctttgatct
//
