LOCUS       sequence-408012-       14771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generate lentivirus to introduce mClover tagged YTHDC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14771)
  AUTHORS   Lee JH, Wang R, Xiong F, Krakowiak J, Liao Z, Nguyen PT, Moroz-Omori
            EV, Shao J, Zhu X, Bolt MJ, Wu H, Singh PK, Bi M, Shi CJ, Jamal N, 
            Li G, Mistry R, Jung SY, Tsai KL, Ferreon JC, Stossi F, Caflisch A, 
            Liu Z, Mancini MA, Li W
  TITLE     Enhancer RNA m6A methylation facilitates transcriptional condensate 
            formation and gene activation.
  JOURNAL   Mol Cell. 2021 Aug 19;81(16):3368-3385.e9. doi: 
            10.1016/j.molcel.2021.07.024. Epub 2021 Aug 9.
  PUBMED    34375583
REFERENCE   2  (bases 1 to 14771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.07.024"; journalName: "Mol Cell"; date: 
            "2021-08-19- 19"; volume: "81"; issue: "16"; pages: "3368-3385.e9"
COMMENT     Sequence Label: pLenti-Ef1a-mClover-YTHDC1-T2A-BSD-siRNA-Resis...
FEATURES             Location/Qualifiers
     source          1..14771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        54..158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(159..227,228..1019)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(377..396)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1190..1778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1679..1698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1932..1949
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2066..2087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2102..2107,2108..2125,2126..2132)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2140..2156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2145..2167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2164..2180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2164..2180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2229..2350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2240..2259)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2294..2313
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2480..2854)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2873..2920)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(2968..3297)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2982..3117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3036..3055)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3282..3302
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3311..3739)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3421..3442)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3633..3652
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3785..4009
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3998..4015
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             4041..4221
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     4726..4742
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4727..4743
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4746..5334
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4852..4863
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5261..5281
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5359..5757)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(5758..5811)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(10203..10913,10914..10916,10917..10919))
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(10245..10266)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     10853..10874
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      10916..10925
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10932..12110)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          10941..11879
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(10964..10984)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    12240..12357
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(12655..12699)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12884..13117
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    13610..13735
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             13782..13962
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(13980..14178)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(14005..14025)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        14180..14559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     14731..14750
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tcgggagatc tcccgatccg tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa
       61 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      121 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      181 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      241 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      301 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
      361 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
      421 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
      481 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
      541 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
      601 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
      661 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
      721 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
      781 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
      841 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
      901 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
      961 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1021 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1081 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1141 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1201 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1261 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1321 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1381 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1441 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1501 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1561 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1621 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1681 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1741 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     1801 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     1861 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     1921 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     1981 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2041 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2101 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2161 acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc gacggtatac
     2221 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2281 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2341 taaacaagtt ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     2401 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     2461 cgaaatctcg tagcacgtgt cagtcctgct cctcggccac gaagtgcacg cagttgccgg
     2521 ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg
     2581 gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt
     2641 ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg
     2701 cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt
     2761 cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg
     2821 tgagcaccgg aacggcactg gtcaacttgg ccatggttta gttcctcacc ttgtcgtatt
     2881 atactatgcc gatatactat gccgatgatt aattgtcaac acgtgctgat cagatccgaa
     2941 aatggatata caagctcccg ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     3001 tcactacttc tggaatagct cagaggcaga ggcggcctcg gcctctgcat aaataaaaaa
     3061 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     3121 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     3181 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     3241 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     3301 ccacagaatt aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     3361 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     3421 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     3481 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     3541 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     3601 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     3661 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     3721 gcgccgctac agggcgcgtg gggatacccc ctagagcccc agctggttct ttccgcctca
     3781 gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca atcctccccc
     3841 ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag acaatgcgat
     3901 gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag ggtcaaggaa
     3961 ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat
     4021 cagcgggttt aaacgggccc tgctagagat tttccacact gactaaaagg gtctgaggga
     4081 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     4141 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     4201 ctggtctaac cagagagacc cagtacagtc cggatgcagc tctcgggcca tgtgatgaaa
     4261 tgctaggcgg ctgtcaaacc tccactctaa tacttctctc tccgggtcat ccatcccatg
     4321 caggctcaca gggtgtaaca agcgggtgtt ctctccttca ttggcttctt ctaccttctc
     4381 ttgctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc tgatccctgg
     4441 ccctggtgtg tagttctgcc aatcagggaa gtagccttgt gtgtggtaga tccacagatc
     4501 aaggatatct tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa
     4561 aaagtggcta agatctacag ctgccttgta agtcattggt cttaaaggta cctgaggtgt
     4621 gactggaaaa cccacctcct cctcctcttg tgcttctagc caggcacaat cagcattggt
     4681 agctgctgta ttgctacttg tgattgctcc atgtttttct aggtctcgag gtcgacggta
     4741 tcgatgcggg gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg
     4801 cggaagaggc cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc
     4861 cgaagggacg tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag
     4921 ccaaggaaag gacgatgatt tccccgacaa caccacggaa ttgtcagtgc ccaacagccg
     4981 agcccctgtc cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag
     5041 ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg
     5101 ggttgcgtca gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc
     5161 ctcataaaga gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga
     5221 agcaatagca tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac
     5281 atagttaaga ataccagtca atctttcaca aattttgtaa tccagaggtt gattaccgat
     5341 aagcttgata tcgaattctt agccctccca cacataacca gagggcagca attcacgaat
     5401 cccaactgcc gtcggctgtc catcactgtc cttcactatg gctttgatcc caggatgcag
     5461 atcgagaagc acctgtcggc accgtccgca ggggctcaag atgcccctgt tctcatttcc
     5521 gatcgcgacg atacaagtca ggttgccagc tgccgcagca gcagcagtgc ccagcaccac
     5581 gagttctgca caaggtcccc cagtaaaatg atatacattg acaccagtga agatgcggcc
     5641 gtcgctagag agagctgcgc tggcgacgct gtagtcttca gagatgggga tgctgttgat
     5701 tgtagccgtt gctctttcaa tgagggtgga ttcttcttga gacaaaggct tggccattgg
     5761 gccaggattc tcctcgacgt caccgcatgt tagcagactt cctctgccct ctccactgcc
     5821 tgtacagtta attcttctat atcgacctct ctcccctcgg tctctgtctc gatcacataa
     5881 tcgctctctt tctctttctc tatcacgtcc tctatctcgc tctctgtctc gtctgttatc
     5941 tctagggcgg tctcgctctc gttcccggtc tctttcacgg ggtctacttc tccggccact
     6001 gacaacagct tgtgtgcgac gaaggaaatc atccaccctc atatcataat catgtactcg
     6061 tttatctctg tatcttgctt catgtggtac tggatgatgt cctgagtaag ggggatgagc
     6121 ttgaggaggt ggagcatggt gctgatagta aggatggtgt ggaggttgtt ccattcctgg
     6181 gtaagggggc attccttgcc aaggtggtgg tggtcccatg ttatgaaatt ccctcacata
     6241 atcattgtag gacccattta aaaacacatc tcggcgaact cctgaaaatc gtctgtccac
     6301 ttcctggtat cgtggatcct ttaaataccc tggtctctgg tgaaactcag ggggatagtc
     6361 aatccttggt ttctttctgc tgttatgaat atcataatct tctggtcgac gccttcccac
     6421 atcccggact ggttctcgac gggatggacg tcctcgtgat cggggctgag aatgcattct
     6481 tctcttgtgt ctcatcttgt ggatcacctg atacaagtca atactttcat cggggggaaa
     6541 cagaagacaa agctgggttc cacattcaag ttcaatttcc tgtccatcac gtccgatctt
     6601 tactggttta tgttcattcc aaggattggt gagatgagcc gacttagtga agggtaattc
     6661 acgcctgcaa atccagtcaa ttttaaagac acctcccagc attttagcac tcattcctgc
     6721 tggaagcacc cagtgtatag gagatcctcc gtgatgtgat tctgaagaaa gtcttgcaaa
     6781 cccttgaaat tttccactct ctctgacaga aaatattaag ataacactcc ttgcagatct
     6841 aaatgcaaga tttaatttct tctcatttac agggagcgtg gaccatacac ccttcgcttt
     6901 ggcaagagac acattctcat ggttgttact ctttatgagg aaaaatcttg catcttgaag
     6961 cacatatttg agtttactgg tttgatcttt tcggacagca cgaacggaag atgataattt
     7021 ctcatgcttc ttttctgaac ctgcatatga ctctgatgca gagcttccac ttctatcaaa
     7081 aacaattgga gatatgcctc tagctctctt cctttccttc tttttctcat ctgatccatc
     7141 tgtgcctgaa ccagatctga cagacccatc tgtgaaggaa acagattcag aaccagagtc
     7201 actggcctca cttcgagtgt cataatcatt tccctcctct ttctggtctc tctcatcctg
     7261 ttcatattct tcttcttcct cctcctcctc ctcctcttcc tcctcctcct cttcctcctc
     7321 ctcctctcca tcttcatcca cctcttcatc ttcttctgca tcttcttcta cttcttcatc
     7381 ttcctccaca tcttcttcca ctccttcctc ctcattctca gtgttgttcc cttgctcatc
     7441 agaagaacca ctgctgccag tctcatggtc agagccatat tcttcagagt tcacttcttc
     7501 cttagaagac tggctggatc tgcttgcacg tctatccact tcaagcccaa ttctctcaga
     7561 accatctggc gtaggagatt tggccctcct ttcaggatcc cttttccgga cacaggtttt
     7621 ttcaggttga ttcttataag gttctctgga ggcactactt gatagacgaa ttttccgatc
     7681 agcatctaga cgcttgtttc tttcagatct ttgatattcc tcatttttat actctgtggc
     7741 tgactttcct tttgtactaa ctattctttt gttattgcta acagatgagc tcagtggctt
     7801 agaaaccagt tgtcttgaat ggacagaagg cttttgtcgt ttggtatcag tagattccat
     7861 tcgatcactt tttctttttg atcccttttt ctcattttta tcttgttcac tctctggatt
     7921 atacagttca tcatcttgtt ctggtacttc agttaaaata tcatccagaa cattaagttc
     7981 tccatctttc tcctcccgac tgtcagccgc cttgtacagt taattcttct atatcgacct
     8041 ctctcccctc ggtctctgtc tcgatcacat aatcgctctc tttctctttc tctatcacgt
     8101 cctctatctc gctctctgtc tcgtctgtta tctctagggc ggtctcgctc tcgttcccgg
     8161 tctctttcac ggggtctact tctccggcca ctgacaacag cttgtgtgcg acgaaggaaa
     8221 tcatccaccc tcatatcata atcatgtact cgtttatctc tgtatcttgc ttcatgtggt
     8281 actggatgat gtcctgagta agggggatga gcttgaggag gtggagcatg gtgctgatag
     8341 taaggatggt gtggaggttg ttccattcct gggtaagggg gcattccttg ccaaggtggt
     8401 ggtggtccca tgttatgaaa ttccctcaca taatcattgt aggacccatt taaaaacaca
     8461 tctcggcgaa ctcctgaaaa tcgtctgtcc acttcctggt atcgtggatc ctttaaatac
     8521 cctggtctct ggtgaaactc agggggatag tcaatccttg gtttctttct gctgttatga
     8581 atatcataat cttctggtcg acgccttccc acatcccgga ctggttctcg acgggatgga
     8641 cgtcctcgtg atcggggctg agaatgcatt cttctcttgt gtctcatctt gtggatcacc
     8701 tgatacaagt caatactttc atcgggggga aacagaagac aaagctgggt tccacattca
     8761 agttcaattt cctgtccatc acgtccgatc tttactggtt tatgttcatt ccaaggattg
     8821 gtgagatgag ccgacttagt gaagggtaat tcacgcctgc aaatccagtc aattttaaag
     8881 acacctccca gcattttagc actcattcct gctggaagca cccagtgtat aggagatcct
     8941 ccgtgatgtg attctgaaga aagtcttgca aacccttgaa attttccact ctctctgaca
     9001 gaaaatatta agataacact ccttgcagat ctaaatgcaa gatttaattt cttctcattt
     9061 acagggagcg tggaccatac acccttcgct ttggcaagag acacattctc atggttgtta
     9121 ctctttatga ggaaaaatct tgcatcttga agcacatatt tgagtttact ggtttgatct
     9181 tttcggacag cacgaacgga agatgataat ttctcatgct tcttttctga acctgcatat
     9241 gactctgatg cagagcttcc acttctatca aaaacaattg gagatatgcc tctagctctc
     9301 ttcctttcct tctttttctc atctgatcca tctgtgcctg aaccagatct gacagaccca
     9361 tctgtgaagg aaacagattc agaaccagag tcactggcct cacttcgagt gtcataatca
     9421 tttccctcct ctttctggtc tctctcatcc tgttcatatt cttcttcttc ctcctcctcc
     9481 tcctcctctt cctcctcctc ctcttcctcc tcctcctctc catcttcatc cacctcttca
     9541 tcttcttctg catcttcttc tacttcttca tcttcctcca catcttcttc cactccttcc
     9601 tcctcattct cagtgttgtt cccttgctca tcagaagaac cactgctgcc agtctcatgg
     9661 tcagagccat attcttcaga gttcacttct tccttagaag actggctgga tctgcttgca
     9721 cgtctatcca cttcaagccc aattctctca gaaccatctg gcgtaggaga tttggccctc
     9781 ctttcaggat cccttttccg gacacaggtt ttttcaggtt gattcttata aggttctctg
     9841 gaggcactac ttgatagacg aattttccga tcagcatcta gacgcttgtt tctttcagat
     9901 ctttgatatt cctcattttt atactctgtg gctgactttc cttttgtact aactattctt
     9961 ttgttattgc taacagatga gctcagtggc ttagaaacca gttgtcttga atggacagaa
    10021 ggcttttgtc gtttggtatc agtagattcc attcgatcac tttttctttt tgatcccttt
    10081 ttctcatttt tatcttgttc actctctgga ttatacagtt catcatcttg ttctggtact
    10141 tcagttaaaa tatcatccag aacattaagt tctccatctt tctcctcccg actgtcagcc
    10201 gccttgtaca gctcgtccat gccatgtgta atcccggcgg cggtcacgaa ctccagcagg
    10261 accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactgatg gctcaggtag
    10321 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
    10381 gcgagctgca cgctgccgtc ctcaacgttg tggcggatct tgaagttagc cttgatgccg
    10441 ttcttctgct tgtcggccgt gatatagacg ttgtggctgt tgaagttgta ctccagcttg
    10501 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
    10561 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt aggtaccgtc gtccttgaaa
    10621 gagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
    10681 ttcatgtggt cggggtagcg gctgaagcag gccacgccgt agccgaaggt ggtcacgagg
    10741 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
    10801 ttggtggcat cgccctcgcc ctcgccgcgg acgctgaact tgtggccgtt tacgtcgccg
    10861 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
    10921 gtggccgtac gtcacgacac ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc
    10981 ttgagaatga accaagatcc aaactcaaaa agggcaaatt ccaaggagaa ttacatcaag
    11041 tgccaagctg gcctaacttc agtctccacc cactcagtgt ggggaaactc catcgcataa
    11101 aacccctccc cccaacctaa agacgacgta ctccaaaagc tcgagaacta atcgaggtgc
    11161 ctggacggcg cccggtactc cgtggagtca catgaagcga cggctgagga cggaaaggcc
    11221 cttttccttt gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt cctccatttt
    11281 gagctccctg cagcagggcc gggaagcggc catctttccg ctcacgcaac tggtgccgac
    11341 cgggccagcc ttgccgccca gggcggggcg atacacggcg gcgcgaggcc aggcaccaga
    11401 gcaggccggc cagcttgaga ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc
    11461 cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc ggccccaaaa
    11521 accgaaatac cagtgtgcag atcttggccc gcatttacaa gactatcttg ccagaaaaaa
    11581 agcgtcgcag caggtcatca aaaattttaa atggctagag acttatcgaa agcagcgaga
    11641 caggcgcgaa ggtgccacca gattcgcacg cggcggcccc agcgcccagg ccaggcctca
    11701 actcaagcac gaggcgaagg ggctccttaa gcgcaaggcc tcgaactctc ccacccactt
    11761 ccaacccgaa gctcgggatc aagaatcacg tactgcagcc aggtggaagt aattcaaggc
    11821 acgcaagggc cataacccgt aaagaggcca ggcccgcggg aaccacacac ggcacttacc
    11881 tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata
    11941 tacggttctc ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag
    12001 tttaccccgc gccaccttct ctaggcaccg gttcaattgc cgacccctcc ccccaacttc
    12061 tcggggactg tgggcgatgt gcgctctgcc cactgacggg caccggagcc aattcccact
    12121 cctttcaaga cctagaaggt ccattagctg caaagattcc tctctgttta aaactttatc
    12181 catctttgca gctagctaat taaccaaact ggatctctgc tgtccctgta ataaacccga
    12241 aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt
    12301 atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg
    12361 tggatgaata ctgccatttg tctgcagaat tggcgcacgc agtgccgatc cgttcactaa
    12421 tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc
    12481 gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac
    12541 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat
    12601 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact
    12661 tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc
    12721 ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca
    12781 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac
    12841 agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt
    12901 taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag
    12961 ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat
    13021 accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct
    13081 tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct
    13141 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac
    13201 tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa
    13261 gatcagcggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt
    13321 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
    13381 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
    13441 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
    13501 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
    13561 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
    13621 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
    13681 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
    13741 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
    13801 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
    13861 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
    13921 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg caaaacgcgc
    13981 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
    14041 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
    14101 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
    14161 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
    14221 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
    14281 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
    14341 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
    14401 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
    14461 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
    14521 taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat ctggcccgta
    14581 catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt cttcatgcaa ttgtcggtca
    14641 agccttgcct tgttgtagct taaattttgc tcgcgcacta ctcagcgacc tccaacacac
    14701 aagcagggag cagatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    14761 accatagggg a
//
