LOCUS       sequence-408051-        1415 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  this mini-donor knockin vector can be used to knock nuclear EGFP 
            into NKX6.1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Haideri T, Lin J, Bao X, Lian XL
  TITLE     MAGIK: A rapid and efficient method to create lineage-specific 
            reporters in human pluripotent stem cells.
  JOURNAL   Stem Cell Reports. 2024 May 14;19(5):744-757. doi: 
            10.1016/j.stemcr.2024.03.005. Epub 2024 Apr 4.
  PUBMED    38579711
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2024.03.005"; journalName: "Stem Cell Reports"; 
            date: "2024-05-14- 14"; volume: "19"; issue: "5"; pages: "744-757"
COMMENT     Sequence Label: mini-donor - NKX6.1-nEGFP
FEATURES             Location/Qualifiers
     source          1..1415
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcaggagtga ttttgtatgt ggccattagg ataccatcac agttaaaatt attttttgca
       61 gtaggttggt acagagccta ctgcgataat ctggaggtct tcatttttca ccgtaaatat
      121 gtctatatgg ctagaagtga attattatag ctgtataaag aaaataaaca tcactttctt
      181 aacaattctg attctctaga taaaatacat aatggaggac aagaataaag ggaggataat
      241 tcaatatctt tgggaaatat gttgcttctt gctgatgctt taggtaaaat atgtgtcaaa
      301 catcatgaat aagcttaaaa atatttgtat taaatgtctt gagcaggaac acagttaaaa
      361 taacatttga aagaatagac atgccaattg tggaaaataa tgtaattgac aatttttcac
      421 gttttaaaaa taaccaatgg taattctagt cttcacttta ctttgggttt atgaagaaca
      481 ttaaaggcga tttgaagttt gtattttaac agatcttttt tttttttttt ttgaagtgca
      541 ccatacattg caagtaaaaa ctgggaggat attttttatc taccatgttt gtacacattc
      601 agatctgaga taagtgaaac aaacaaatgt ttaaaagtga aaaggagaac agaagtaatt
      661 ggggcaaatg ctgtgctttc cccccacaat ctataccgaa aagcaagtac gtgtcaggtt
      721 gtttgaaatt tcttaattta agaaagtaaa taaggaataa aatcacagcc actgtggggc
      781 aacttcatga agggggaaag ttttcaactt caaactggaa ggcaggaaag tgaactcact
      841 tttagggacg ctcattttta agtttccgag ttttgttttt acttttgaga actcgctcac
      901 tgacaacctc aatactcttg tgttaccatc gctatggcaa cggcagtcag tttgttggtg
      961 ccagttactc agggggaact tggggcgacg gagactgact ttgacggccg agaaaaggga
     1021 gggctgcgag ggagggcagc aggccttcgg ggggccgcgg gagggccatc gagatgcggg
     1081 agtggtagca tggcctggat ctgctcgttt aattcctcgc ccttgcctcc cctctcctcc
     1141 cttttctccg ccctcaggtc tggttccaga accgccggac caagtggagg aagaagcacg
     1201 ctgccgagat ggccacggcc aagaagaagc aggactcgga gacagagcgc ctcaaggggg
     1261 cctcggagaa cgaggaagag gacgacgact acaataagcc tctggatccc aactcggacg
     1321 acgagaaaat cacgcagctg ttgaagaagc acaagtccag cagcggcggc ggcggcggcc
     1381 tcctactgca cgcgtccgag ccggagagct catcc
//
