LOCUS       sequence-408052-        1990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  this mini-donor knockin vector can be used to knock nuclear EGFP 
            into NKX6.1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Haideri T, Lin J, Bao X, Lian XL
  TITLE     MAGIK: A rapid and efficient method to create lineage-specific 
            reporters in human pluripotent stem cells.
  JOURNAL   Stem Cell Reports. 2024 May 14;19(5):744-757. doi: 
            10.1016/j.stemcr.2024.03.005. Epub 2024 Apr 4.
  PUBMED    38579711
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2024.03.005"; journalName: "Stem Cell Reports"; 
            date: "2024-05-14- 14"; volume: "19"; issue: "5"; pages: "744-757"
COMMENT     Sequence Label: mini-donor - NKX6.1-nEGFP
FEATURES             Location/Qualifiers
     source          1..1990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgaacgccgc cgcccgccgc accttcccgg ctccggcctc cacctctggg gccgcgaggg
       61 gcgcggggac cgcgtgcacg ccgcgcctgc ccgctcctcg gggcccggag gacccggccc
      121 tgcctgcggg gaccctttgc tatttttgag atgtacatat ctattttttt aaccttgaag
      181 ttgtgggagg ggatgtaggg gagagagact ccggggttga taaggcaaga aagaccacca
      241 aatgcactgc gtaaatgtcg ctactgaaac ccgcagccgg gccacggtcc cagccgcgtc
      301 cccataggcg ggcagcggcc tcagcgcccg ggtccccctc gcccccagat tttgcccccg
      361 cgggcggcgg ggtagggggc gccgagaggt gctgtggaag ggttcacgct caccgcatgg
      421 gggacctcgg agcgaagcgg gcgggcgctg ccacagatgg gaagagggac gtttcccccc
      481 ccacacactg ctgtgcaact aaagtaacct gttgaaggct ctttgtaaat aaatcgtgag
      541 ttacactgac tagaagttac tttattgtga atatttaaaa tgtaaaatgc ttttttgaat
      601 ttcgtataaa aagatggact ttcttccttc tcctcctccc ctttgaagag cgactcaagc
      661 cacaccttcc cttttatttt gaacttaatt tgagaagcgt agaatagaat gtccccacct
      721 tctccgtccc ctcctctcgt tcctctttcc cgtttggatt tgcagaatct cacctttgcc
      781 cttcatctct tctcgccctg ccctgccctg cccaccccct ggatctacac tgcgctcccg
      841 ccccccggac ttgaagtgga agacgtggga accagggagg gcctctcacc tgcctccctg
      901 ttcctctctg aaattgcaca gtcgtttgtt gctatttatt agtgatttaa agaaaatatt
      961 ggggagggag gggctacaat agcttgtatt taactccttt ctgataaaaa tgcgtgttaa
     1021 agtaggtggt gggaattgtt acaaccgctc tgggtctgaa acataaaaat tcatttagtt
     1081 actgtaattg aatttgaagt gttttgtatc ataagaatac tatagaatat gtaaattgtt
     1141 ttctttttac agctaataat ggtgatatat tagcatcttt aacctttatt aatttatata
     1201 aggaattcgg tttgtaaaga gaagaaaacc ccctttgcaa gaccagttaa atgtaatgca
     1261 ctttctgttt caaaggataa atcaaattaa aaaaaacagt ttgaagacgt ctgttgcctt
     1321 gaatgaaagg gtacagatca tctgatctca tcagagatct caaataataa aagtcaagac
     1381 cacaaaaaaa atggtatatc ggtttcttgg gtgaaatagc tgttacttaa tttttttttc
     1441 tttggatgag tgatgtcaca tttctcattc tcctggatag gaaaataaaa taaatgcagc
     1501 cccactcatg gtgattcttg gagagactag tctagctagc tagctgcttc tgatgtctgt
     1561 gttgtctaat ttcttcctga tcgctttttc cttttccagg aagtccctgg ggttgggggg
     1621 cagttgggaa gagctcattt ccatatatcc aagatagctc ctcagtcagc ccctattcga
     1681 ttagcgtaga ggaggcagcg gagctcgctg gttgagtagc actgctgttt gatgctaata
     1741 tttctccccc gtagctgtaa gactgcagaa attgtggaaa gacagaagcc ttgattttac
     1801 taaacattac tgttgaaaga tgaatttaaa gaaagcttcc tttaactggt gtaaaaatat
     1861 tttagggtta agaaagcaat gtcatagctt ctccagctgc cgaatctgaa gaaaaaaaaa
     1921 attgggaagg ccagggctgt atcactgttt tgggatcttt ttgtttttag acactcggtt
     1981 tcaggctcag
//
