LOCUS       sequence-408062-        9333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, AFF1_RAD51B from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_AFF1_RAD51B
FEATURES             Location/Qualifiers
     source          1..9333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     168..187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     421..438
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    506..640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(530..549)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     584..603
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(646..841)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      660..795
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    900..921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(936..941,942..959,960..966)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    974..990
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     979..1001
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     998..1014
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     998..1014
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1357..1376
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1375..1430
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1476..1495
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4042..4752,4753..4755,4756..4758))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4084..4105)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4433..4452
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4692..4713
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4755..4764
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4840..5043)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4869..4889)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5044..5347
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5604..5648)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5833..6066
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6426..6543
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7035..7160
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7207..7464
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7466..7845
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7876..7980
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7981..8049,8050..8841)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8199..8218)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9012..9333,1..267)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
       61 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      421 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
      481 cgttggccga ttcattaatg cagcggatcc agacatgata agatacattg atgagtttgg
      541 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      601 tgctttattt gtaaccatta taagctgcaa taaacaagtt agctttttgc aaaagcctag
      661 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
      721 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
      781 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
      841 cggatccgct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      901 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      961 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     1021 tacgccaagc ttgggctgca ggtcgaggga tctccataag agaagaggga cagctatgac
     1081 tgggagtagt caggagagga ggaaaaatct ggctagtaaa acatgtaagg aaaattttag
     1141 ggatgttaaa gaaaaaaata acacaaaaca aaatataaaa aaaatctaac ctcaagtcaa
     1201 ggcttttcta tggaataagg aatggacagc agggggctgt ttcatatact gatgacctct
     1261 ttatagccaa cctttgttca tggcagccag catatgggca tatgttgcca aactctaaac
     1321 caaatactca ttctgatgtt ttaaatgatt tgccctccca tatgtccttc cgagtgagag
     1381 acacaaaaaa ttccaacaca ctattgcaat gaaaataaat ttcctttatt agccagaagt
     1441 cagatgctca aggggcttca tgatgtcccc ataatttttg gcagagggaa aaagatctgc
     1501 tagctcgagc agctgaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     1561 agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa aagcagatct
     1621 tgtcttcttt gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta
     1681 agatctacag ctgccttgta agtcattggt cttaaaggta ccgtaatctt atctagaacc
     1741 ggttcacacc gggtgctcgt ccaaggtttc tgtccgtata cccgtggccc agttagtagg
     1801 agggagtgca gaactcaaaa ctgtagaatg catctgcggg ggacacatgc ttactgaggg
     1861 caacaggcgg ggattcctgg tctttctcaa aacaacgtca gggataagga atgttggact
     1921 gggatgtgaa tcggcggctg accaacattc actccgcgga cttacccgtg ctaccgtgcg
     1981 gactacctgc aggacaagtc cttcctcttt tatcgtgtaa acaaaggatg taaacggtgc
     2041 aagtggactc ttcgctatga gtatctgtct ccgctcggag tcgaggtact gcagaataag
     2101 gcgagtattt acgctatgag accaagtatt ccccaacgcg gcaattacac atgagctccc
     2161 actggttcct tcagacagtg aaagatcgtc ggcgggggaa accaaatctg cttgactcgc
     2221 caaagcaccg gaaagatggg tcgttatctg gttagtcagt ataacaggaa tgctaaactc
     2281 ttcggcgagg tacttgagag aactggcctc tcgtgccagg aatttattcc gttctttcag
     2341 gtttccttgc agttgagcat caaattcttt tctaacgacg ctagccacac tgtcaagtat
     2401 cacgagcttt attcccttac ttataatctc ctcctccaaa ctctctatcc tctgcaacac
     2461 ctcgtcgcat gtgagctcgc ggtaaaggtg taccttgcta gaggtcaaca gcagcttctc
     2521 ttcagtgttg aaatacctcg gaaacctgga ctccgctatc tccaccagtc tctccgctga
     2581 aaatgcagat tcagtatcta tgtatacaac ggccccctcg agtcctccca tattcgtggg
     2641 aagtgttgcg agtatagaca tcattataca gaactgcgtc ttaccacagc ccggcggccc
     2701 agttatctcc gtcaaggagc cgcaggccac gcctccatgc aaggcttcgt ccaatgcgga
     2761 caatgtggtg ctcaaaaacg caggagagaa atctgcactg cgttgggctt tgatgccata
     2821 cgcggtctgc atcttcggag cacacgcccg tgataccata cagagcaatt cgtgcacccc
     2881 tcggtaggac aatccggtca ccttcatcag ctccaaaggg ctcaggcaca ggaagtcttc
     2941 aactgattgt gacggcattt tcagtttggt cagctttgcc tttgccggca ccttgagatc
     3001 tgttttctcg aaggtctgtt gtctatagtc ttcggggagc ggcttcaact cagggctttc
     3061 tgatggggcc tggtcctgac cgtccatcgg ccttacgtat gctgttggct tttgctgcat
     3121 cgcgacagac tttgatggca gagatggggg tggaaatgtt tgggatggtg gctgggcagg
     3181 ggcaacgagg gagtcctgtg gtgtctcttt atcatgtacc ttgaccgcca gatctttagg
     3241 agactttgcc gggcaatatc ctttgctatt gtttgaggag ccgtgcacct ttgagcttcc
     3301 ctgagtgcgg ggaagcgtct gttggttgga gtggatggga gagagtggtg gaacggggga
     3361 cggcaagctg ataagaggag agagttcgcg ctctggcgca ctatccgtaa cggaagcgca
     3421 gtggtctccg tctgctcttc tatctccctt tttgtggtga ctggaaccaa atccttcctg
     3481 gccgagtcga tcttgggtca ggtgctgact atctgggggc ccgcaggatt ttgcgtggag
     3541 tgatggcatc ggttccgtgc ggggctgggc cattttggga ttatgagaaa tgttacctac
     3601 agacaatggg ccggatgcag gggtgtgaat gctctgatga tgtacactcg tgtgaaatga
     3661 gcttgacggg atgctgctgc ccttatcagg tatcaagggg tatttaggtt tcccgaggcg
     3721 attttcactg gcgtcaaggc ggtgggtgtg actcttagtg ctcaggaact cctttacttc
     3781 ctcataatta cccaacatgt tttgtattcg ggaactcaat tcatctccct tggctgtctt
     3841 ataaggctct ccgaagaggg gaattttttc agggaatgcc tccttctctt ggtgagcttc
     3901 ctgattgcgc cgttcctttt ctcgtattct gagcaagttc cgatcatcgt tataaagact
     3961 gttcccggat gagttcactc tctcagtaaa agccattccc cgggaagcgg ccgcacttcc
     4021 cccggagcct ccgcttccgc ccttgtacag ctcgtccatg ccgagagtga tcccggcggc
     4081 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt
     4141 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
     4201 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
     4261 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
     4321 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
     4381 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
     4441 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
     4501 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
     4561 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
     4621 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
     4681 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
     4741 ctcgcccttg ctcaccatgg tggcgaccgg tggatcccgg gcccgcggta ccgtcgactg
     4801 cagaattcgg ttcactaaac gagcccggtt cactaaacga gctctgctta tatagacctc
     4861 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     4921 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     4981 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     5041 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     5101 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     5161 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     5221 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     5281 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     5341 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     5401 ataactagca cgcgttcgaa gcttgagctc ggtcccctcg gggttgggag gtgggtctga
     5461 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact
     5521 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa
     5581 tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt
     5641 tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa
     5701 ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat
     5761 ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa
     5821 ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc
     5881 tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc
     5941 ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc
     6001 agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa
     6061 gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt
     6121 gctactccta atggttcaat ttttactact ttatatttat ataattcact tctccaattg
     6181 tccctcatat ctcctcctta tggtgtttta ctaaactttt ccatgttcta atcctcatcc
     6241 tgtctacttg ccacacaatc atcacctgcc atctgttttc cataatccct aatgatcttt
     6301 gcttttcttc ttggcactac ttttatgtca ctattatctt gtattactac tgccccttca
     6361 cctttccaga ggagctttgc tggtcctttc caaagtggat ttctgctgtc cctgtaataa
     6421 acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
     6481 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
     6541 aaattgtgga tgaatactgc catttgtact gctgtcttaa gatgttcagc ctgatctctt
     6601 acctgtccta taattttctt taattcttta ttcatagatt ctactactcc ttgactttgg
     6661 ggattgtagg gaatttccag gtctgaagat cctgctgctt gctgtgcttt tttcttactt
     6721 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
     6781 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
     6841 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
     6901 aggccagtat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
     6961 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
     7021 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
     7081 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
     7141 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
     7201 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     7261 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     7321 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     7381 gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc tcgccactcc
     7441 ccagtcccgc ccaggccacg cctcccatgg taatagcgat gactaatacg tagatgtact
     7501 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     7561 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     7621 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     7681 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     7741 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     7801 accccgtaat tgattactat taataactag tcaataatca atgtcgaccc aggtggcact
     7861 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     7921 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     7981 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     8041 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     8101 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     8161 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     8221 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     8281 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     8341 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     8401 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     8461 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     8521 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     8581 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     8641 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     8701 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     8761 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     8821 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     8881 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     8941 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9001 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9061 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     9181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9301 ttaccggata aggcgcagcg gtcgggctga acg
//
