LOCUS       sequence-408063-       10593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, ASCC3_GRIK2 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 10593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_ASCC3_GRIK2
FEATURES             Location/Qualifiers
     source          1..10593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(5354..6064,6065..6067,6068..6070))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5396..5417)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5745..5764
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6004..6025
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6067..6076
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6152..6355)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6181..6201)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6356..6659
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6916..6960)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7145..7378
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7738..7855
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8347..8472
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8519..8776
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8778..9157
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9188..9292
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9293..9361,9362..10153)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9511..9530)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10324..10593,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcatg
     1801 ccatcgtttc tttacctggc agacgtctat cgttgaaggt gtgcatgttg atcacctctt
     1861 cggtcttaac gataaccggc gcctgaggtt tgtgctttaa gcgccgctgg cacttcaggc
     1921 tcatacgcag ttcttcaacc atggcgctgc agaagctgcg cttctctaac tgtgcattct
     1981 tcttgctttt atacagaaat tcgcccacgg caacaaagac tgacagcacc agtcccgcag
     2041 ccagtacgat aaaaatgcca ccgatgttct gtacacccaa ggcggacgcc tccttcgact
     2101 cctcctcggg gcacccatta ccacgccacc acttctcttt catcatgtgc agtttgccct
     2161 cttcttgcag ctgcaaaatc gcaatggtga ttttatcacg gtatgggcta cccatcggtg
     2221 tgcccacgcc atagccctta gagtcaatca gaccgccaat ttgcgtcaag ttgcaattgc
     2281 gttgggtcac gaactcgatg gtggttgatt ccatcaggaa tgcgtaatcg ctcgtgagga
     2341 cacgctgaat gccctcttcg ttgcttttaa ctaacaccga ttgacgacgg ctgctcatga
     2401 atgcccacat cttgtcgtac gtagaaattt tgctcttttt gaagaaggtc atggttgcac
     2461 cgtcttccac cgcaccatat tcaattttgg tctgttttgc tagatcgtcg gcactatcaa
     2521 tcgggctctc catacgttcc acggtcagga acgcggccag gttcgcggta tagctgctga
     2581 tgatgatcag ggtgaagaac caccaaatgc caccgacgat acgggtgctc agagctttcg
     2641 gcatcagctc gctaccctgc tgcatcaaag cgcccacacc aaaccagaag ctattcagca
     2701 aggtgaagtt gttctccacg acgtcgctat ctggattaca cggatgcggg ttataccact
     2761 catacgggct gaaacgagcg ataacaaaca gaacacaaga cacgcccaga tacgctagca
     2821 ggatatacat ccagatatcc ggggacaacg ggttcagaaa gctaaaaaca cccggattgg
     2881 taccattcgg tttacggtac aaaatgctaa tacccagggt cataaacggc ttagaaaagt
     2941 cgatgacttt ttcgcgcacg taagtaatgg caagcggagc aactgctaag tccgccttgt
     3001 gatcgatcaa ttctctcacc atgccattcc actggccatt tgcgtcgtcc tgggcaccgt
     3061 atttgccgtc ctcgaccagg cggatctcgt acgtaaaacc cagaatggtg gacaattcac
     3121 gcagcagatc aatgcagtag ccctcgaaac gatcgttgcc gtacagcggt ttgtcgcttt
     3181 ttttgaacag aacgtacggc tcctcaagaa tggtggtaac gatcagggaa cggttggaca
     3241 gagagtcggt aatgttcgcc ggcttacctt tttggctctc cgtcatattc aggccagacg
     3301 ccgggtccca cgtaccaatt ttttccaaac cttcttcctt caaagaaata acatccagat
     3361 cgaagtcggt gcgaaggcca ttggttttgt taaaggtaat acgacccgtc aagccttccc
     3421 aatgtgcctc tttgatcagc gacatgaaac gggtgccgaa acgccaaggc ttgtgacggt
     3481 tacattgcaa ggagctaacc gtcatctgcg gaaactgctg caccgccacg ctcaccacgt
     3541 gaactgcgtc gtacatcaac gccgcgtcgg tttgggtacg caggatctcg gacttgctca
     3601 gctgcatgtc tcctgtcagt tctttgacga tgatacccag cggttccaag cggcggctaa
     3661 aataatcagt catttccgca gcaagggcct tcatcggagc cacatacacg atcttgaact
     3721 cattcttctt gatcacacct tgttggaagt gctgacgaat ttcgtgtaac accgtcagca
     3781 tggcgatgtt agtcttaccc gcaccggtcg gtgcgcagat taacatgttc tcgttggtgt
     3841 tatacgctgt ttcaaatact atgctctgga tacggttcag acgtttcata cccttgaacg
     3901 ccagctgacc gatttcgtcc aggtcttgaa tgtaaaccgg cttctcttca aaactcagcg
     3961 gcatcggttc gctatacggg atacgcacct cctcgtacag cttgttattt tcacgctgga
     4021 taccttccgg taaaatcatc ttcgcacccg caatgaatgc gctggtcttc atcgcttccg
     4081 cctgggaatc gtaaacatgt ggataatgga tcttttcaac atccgcatct cgttgacgac
     4141 taaggatcgg cacagatctc gcgttcagga gcgcctgctc gcgctggatg cgcagttctt
     4201 tcggatcaaa gcacatcagg ccttcgctga cctccagatc ttcaccagct tttttttcac
     4261 gacgggcaat gcgcttctcc tcccgacgat actgcttcat cagttgcttc tcttgctcgc
     4321 tttgaatggt tacctggcag ccgtagttcg gtttagcgtt ttcacctaga atctttttgc
     4381 agttgtcttg cagagcctgg aaacgatgat cattgctgga gttcaaaaaa cgatcaacaa
     4441 tggtgatgcg gttttgcagg agtttttcga ttaactccag accttccgga cccagcaact
     4501 caaaaagctc atcctgcagc tcgtcaccac tctttatgct ggccagcatg tcgtacaggg
     4561 tacaacacag atcctcaaca cgcgggacct cggtcatctc cttcagggtg ctgttcaggt
     4621 acttttcaac ttcacaccac agaaaagatc cgttagtttt ctccaccggc ttcagttccg
     4681 gggtgcaggc ttcttgcagg tgctcgttca ggaatttttt gtagtccagg gagatggttt
     4741 tttgtgtctc gccgttaatc ggcagctcgt caaagtggtc caaatcgtgc atgtcgaagg
     4801 agaacgccag gtttttgcca aagaacacgc ggtcaccatg ctccttctca gtcatctgca
     4861 ctaaggccgt caaatcgtct tgcgaaaaat gggaaataat gcgattggtc gcattgcacg
     4921 ccgcagtcgc ggacgagctc ggaaacggac caaacatctg tttgatcgct ttggtttctt
     4981 tatgaccaac gctatccttc agatggaagg tcatgaacaa gaacgcggca ccgctttcga
     5041 tcgcctcacg gccgttatcg gtgccgacga tctgtttcgc tgcgtgcagg atgtccttga
     5101 ggtcctcatt gatgctctgc attttcgact tttccagctt ctcgttgaga aatttgataa
     5161 tctttttcca agtcaggcca aggtccagaa cttgttcgtg cagcttgctg cgtttgatct
     5221 tcaaatccgc aacttcctcg ttataattat cctgcttggt aacattagaa aaggaacgta
     5281 gagctcctgt taacctgggc aatgccattc cccgggaagc ggccgcactt cccccggagc
     5341 ctccgcttcc gcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     5401 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg
     5461 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     5521 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
     5581 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
     5641 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     5701 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     5761 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     5821 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg
     5881 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     5941 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     6001 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     6061 tgctcaccat ggtggcgacc ggtggatccc gggcccgcgg taccgtcgac tgcagaattc
     6121 ggttcactaa acgagcccgg ttcactaaac gagctctgct tatatagacc tcccaccgta
     6181 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     6241 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     6301 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     6361 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     6421 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     6481 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     6541 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     6601 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     6661 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
     6721 cacgcgttcg aagcttgagc tcggtcccct cggggttggg aggtgggtct gaaacgataa
     6781 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
     6841 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
     6901 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
     6961 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
     7021 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
     7081 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
     7141 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
     7201 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
     7261 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
     7321 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
     7381 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
     7441 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
     7501 atctcctcct tatggtgttt tactaaactt ttccatgttc taatcctcat cctgtctact
     7561 tgccacacaa tcatcacctg ccatctgttt tccataatcc ctaatgatct ttgcttttct
     7621 tcttggcact acttttatgt cactattatc ttgtattact actgcccctt cacctttcca
     7681 gaggagcttt gctggtcctt tccaaagtgg atttctgctg tccctgtaat aaacccgaaa
     7741 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     7801 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
     7861 gatgaatact gccatttgta ctgctgtctt aagatgttca gcctgatctc ttacctgtcc
     7921 tataattttc tttaattctt tattcataga ttctactact ccttgacttt ggggattgta
     7981 gggaatttcc aggtctgaag atcctgctgc ttgctgtgct tttttcttac ttttgttttg
     8041 ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac
     8101 acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag
     8161 ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagt
     8221 attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt
     8281 tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt
     8341 aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg
     8401 cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct
     8461 gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg
     8521 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     8581 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     8641 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     8701 gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact ccccagtccc
     8761 gcccaggcca cgcctcccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     8821 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     8881 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     8941 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     9001 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     9061 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     9121 attgattact attaataact agtcaataat caatgtcgac ccaggtggca cttttcgggg
     9181 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     9241 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     9301 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9361 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     9421 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9481 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9541 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9601 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9661 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     9721 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9781 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9841 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     9901 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9961 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    10021 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    10081 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    10141 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    10201 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    10261 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10321 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    10381 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    10441 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    10501 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10561 tgctgccagt ggcgataagt cgtgtcttac cgg
//
