LOCUS       sequence-408069-        9483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, GFM1_MLF1 from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_GFM1_MLF1
FEATURES             Location/Qualifiers
     source          1..9483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(113..217)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        248..627
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             629..886
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    933..1058
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1550..1667
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2027..2260
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2445..2489
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2746..3049
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3050..3253
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3204..3224
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3329..3338
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3335..3337,3338..3340,3341..4051)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3380..3401)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3641..3660)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3988..4009
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6748..6767)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6813..6868
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6867..6886)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7229..7245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7229..7245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7242..7264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7253..7269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7277..7283,7284..7301,7302..7307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7322..7343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7402..7597
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7448..7583
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7603..7737
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7640..7659)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7694..7713
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7805..7822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7976..8564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8056..8075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8735..9483,1..43,44..112))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9358..9377
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
       61 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      181 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      241 ctgggtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      301 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      361 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      421 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      481 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      541 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      601 tacgtattag tcatcgctat taccatggga ggcgtggcct gggcgggact ggggagtggc
      661 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
      721 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      781 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      841 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
      901 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
      961 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1021 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1081 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1141 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatactggc ctgttagaaa
     1201 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1261 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     1321 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     1381 aaaaagcaca gcaagcagca ggatcttcag acctggaaat tccctacaat ccccaaagtc
     1441 aaggagtagt agaatctatg aataaagaat taaagaaaat tataggacag gtaagagatc
     1501 aggctgaaca tcttaagaca gcagtacaaa tggcagtatt catccacaat tttaaaagaa
     1561 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     1621 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     1681 gggacagcag aaatccactt tggaaaggac cagcaaagct cctctggaaa ggtgaagggg
     1741 cagtagtaat acaagataat agtgacataa aagtagtgcc aagaagaaaa gcaaagatca
     1801 ttagggatta tggaaaacag atggcaggtg atgattgtgt ggcaagtaga caggatgagg
     1861 attagaacat ggaaaagttt agtaaaacac cataaggagg agatatgagg gacaattgga
     1921 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1981 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     2041 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     2101 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     2161 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2221 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2281 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2341 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2401 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2461 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2521 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2581 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2641 acctcccaac cccgagggga ccgagctcaa gcttcgaacg cgtgctagtt attaatagta
     2701 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2761 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2821 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2881 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2941 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     3001 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     3061 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     3121 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     3181 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     3241 tataagcaga gctcgtttag tgaaccgggc tcgtttagtg aaccgaattc tgcagtcgac
     3301 ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt
     3361 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     3421 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     3481 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     3541 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     3601 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     3661 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     3721 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     3781 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     3841 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     3901 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
     3961 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4021 gatcactctc ggcatggacg agctgtacaa gggcggaagc ggaggctccg ggggaagtgc
     4081 ggccgcttcc cggatgcgtc tgttaggtgc tgcggccgta gccgccttgg gacgcggccg
     4141 tgcccctgca tcattagggt ggcagcgcaa gcaggtaaac tggaaggcat gtcgttggtc
     4201 tagtagtggt gtaattccca atgagaagat tcgtaacatc ggaatcagcg cgcatattga
     4261 ctcaggcaaa acgactttaa cagaacgtgt actttactat acggggcgta ttgcaaagat
     4321 gcacgaggtg aagggcaaag acggagtagg agccgtgatg gactcgatgg aacttgaacg
     4381 ccaacgtggg attacgatcc aatcagcagc aacctatact atgtggaagg acgttaacat
     4441 taatattatt gatacgccag gccacgtgga tttcaccatt gaggtcgaac gtgcgttgcg
     4501 cgttttagac ggcgccgtgt tggtcctttg cgcagtagga ggggttcaat gccaaacaat
     4561 gacagttaac cgccagatga agcgttataa cgttcccttc ctgaccttta ttaacaagct
     4621 ggatcgtatg ggatctaatc ctgcacgcgc tttacagcag atgcgctcca aattgaacca
     4681 caacgcggca tttatgcaaa ttccaatggg tttagaggga aatttcaaag gaatcgttga
     4741 tttaattgaa gaacgcgcga tctacttcga tggcgacttt ggtcaaattg tgcgctacgg
     4801 ggagatcccc gcggagttac gcgccgcagc gacggatcat cgtcaggaac ttattgaatg
     4861 tgtggccaat agtgatgagc aactgggcga gatgtttctg gaggagaaaa ttccgagtat
     4921 ctctgatctg aaacttgcaa ttcgccgcgc caccttgaag cgttctttta cccctgtctt
     4981 ccttggctcg gcgctgaaga acaagggcgt ccagccattg ttggatgctg ttttagagta
     5041 cttaccgaat ccatcggaag tccagaatta tgccatctta aataaggagg atgactcaaa
     5101 ggaaaagacg aagattttga tgaactcttc ccgtgacaat tcccacccat ttgtcgggtt
     5161 ggcctttaag ttggaagttg gccgcttcgg acaattaaca tacgtccgct cttatcaggg
     5221 agagttaaaa aagggagaca caatttataa cacgcgtacc cgtaaaaagg tacgtttaca
     5281 acgccttgcg cgcatgcatg ctgacatgat ggaggacgtg gaagaggtgt acgcggggga
     5341 catctgtgct ctttttggca ttgactgtgc atcaggcgac acttttactg ataaagccaa
     5401 cagtggcctg tccatggaaa gtattcacgt tccggacccc gtcattagta ttgccatgaa
     5461 acccagcaac aagaatgacc tggagaagtt ttccaaagga attggccgct tcacacgcga
     5521 agacccgaca tttaaagtct attttgatac agaaaataaa gaaactgtca tctcaggcat
     5581 gggcgagttg cacttagaga tttatgcaca acgtctggag cgtgagtacg gctgcccgtg
     5641 catcacaggt aagcccaaag tggccttccg tgagaccatc acagctccag ttcctgagtc
     5701 tattcttgca catcgtgaaa atatgcgtca aatgatccgc tccttctcag agcccttcgg
     5761 ccgtgacttg ctgtcgatct cggacggccg tggtcgtgcg cataatcgtc gcggccacaa
     5821 tgacggagag gattcgctta cagctacctc atgttctttg gtaccgtttg gtgactttgg
     5881 gggcatgcat actgatgttt cttcttttca gaccatggac cagatggtgt cgaacatgcg
     5941 caactatatg caaaaattgg aacgcaactt tggtcaactt agcgttgacc cgaatggcca
     6001 ctcgttttgt tctagctcgg taatgactta ctcgaaaatc ggggacgagc caccaaaggt
     6061 ttttcaagcc tctacacaaa ctcgtcgtgc accgggtggg atcaaagaga ctcgcaaggc
     6121 aatgcgcgac agtgattcgg gactggaaaa gatggccatt gggcaccaca tccatgaccg
     6181 cgcacatgtg atcaagaaga gcaagaataa gaaaactggc gacgaggagg taaatcaaga
     6241 gtttattaat atgaacgagt ccgatgccca tgccttcgat gaagaatggc agtctgaggt
     6301 gctgaagtat aaaccaggac gccataactt gggaaatacg cgcatgcgct cagttggaca
     6361 tgagaatcca ggtagtcgcg aactgaagcg tcgtgaaaag cctcagcaga gcccagcgat
     6421 cgagcacggg cgtcgttcaa acgtacttgg ggataagctg cacattaaag ggagctcagt
     6481 gaaaagcaac aaaaaatgaa ccggttctag ataagattac ggtaccttta agaccaatga
     6541 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     6601 taattcactc ccaaagaaga caagatctgc tttttgcctg tactgggtct ctctggttag
     6661 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6721 aaagcttcag ctgctcgagc tagcagatct ttttccctct gccaaaaatt atggggacat
     6781 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     6841 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     6901 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     6961 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     7021 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     7081 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     7141 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     7201 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7261 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7321 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7381 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     7441 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7501 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7561 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
     7621 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7681 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
     7741 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7801 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7861 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7921 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7981 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8041 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8101 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8161 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8221 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8281 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8341 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8401 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8461 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8521 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8581 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8641 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8701 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8761 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8821 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8881 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8941 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9001 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9061 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9121 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9181 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9241 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9301 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9361 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9421 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9481 cca
//
