LOCUS       sequence-408073-        7405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a mutant PDGFRb gene (559-562 deletion). Used for DNA 
            delivery using Adeno-associated Virus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Shima Y, Sasagawa S, Ota N, Oyama R, Tanaka M, Kubota-Sakashita M, 
            Kawakami H, Kobayashi M, Takubo N, Ozeki AN, Sun X, Kim YJ, Kamatani
            Y, Matsuda K, Maejima K, Fujita M, Noda K, Kamiyama H, Tanikawa R, 
            Nagane M, Shibahara J, Tanaka T, Rikitake Y, Mataga N, Takahashi S, 
            Kosaki K, Okano H, Furihata T, Nakaki R, Akimitsu N, Wada Y, Ohtsuka
            T, Kurihara H, Kamiguchi H, Okabe S, Nakafuku M, Kato T, Nakagawa H,
            Saito N, Nakatomi H
  TITLE     Increased PDGFRB and NF-kappaB signaling caused by highly prevalent 
            somatic mutations in intracranial aneurysms.
  JOURNAL   Sci Transl Med. 2023 Jun 14;15(700):eabq7721. doi: 
            10.1126/scitranslmed.abq7721. Epub 2023 Jun 14.
  PUBMED    37315111
REFERENCE   2  (bases 1 to 7405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2023-06-14"; volume: "15(700):eabq7721. doi"; pages: "
            10.1126/scitranslmed.abq7721. Epub 2023 Jun 14"
COMMENT     Sequence Label: pAAV-CMV-PDGFRb559-562del-HA
FEATURES             Location/Qualifiers
     source          1..7405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   12..141
                     /label=AAV2 ITR
     enhancer        282..585
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        586..789
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     740..760
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2358..2504
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KEIR"
     CDS             4128..4154
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4165..4182)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4171..4378
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4466..4595
                     /label=AAV2 ITR
     primer_bind     complement(4614..4631)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4614..4630)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4623..4645)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4772..5227
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4859..4878)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5069..5090
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5253..5357
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5358..5426,5427..6218)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5576..5595)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6389..6977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6878..6897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7265..7286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7301..7306,7307..7324,7325..7331)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7339..7355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7344..7366
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7363..7379
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7363..7379
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccttaattag gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt
      181 agccatgctc taggaagatc ggaattcgcc cttaagctag ctagttatta atagtaatca
      241 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      301 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      361 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      421 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      481 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      541 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      601 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      661 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      721 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      781 agcagagctg gtttagtgaa ccgtcagatc ctgcagacac catgcggctt ccgggtgcga
      841 tgccagctct ggccctcaaa ggcgagctgc tgttgctgtc tctcctgtta cttctggaac
      901 cacagatctc tcagggcctg gtcgtcacac ccccggggcc agagcttgtc ctcaatgtct
      961 ccagcacctt cgttctgacc tgctcgggtt cagctccggt ggtgtgggaa cggatgtccc
     1021 aggagccccc acaggaaatg gccaaggccc aggatggcac cttctccagc gtgctcacac
     1081 tgaccaacct cactgggcta gacacgggag aatacttttg cacccacaat gactcccgtg
     1141 gactggagac cgatgagcgg aaacggctct acatctttgt gccagatccc accgtgggct
     1201 tcctccctaa tgatgccgag gaactattca tctttctcac ggaaataact gagatcacca
     1261 ttccatgccg agtaacagac ccacagctgg tggtgacact gcacgagaag aaaggggacg
     1321 ttgcactgcc tgtcccctat gatcaccaac gtggcttttc tggtatcttt gaggacagaa
     1381 gctacatctg caaaaccacc attggggaca gggaggtgga ttctgatgcc tactatgtct
     1441 acagactcca ggtgtcatcc atcaacgtct ctgtgaacgc agtgcagact gtggtccgcc
     1501 agggtgagaa catcaccctc atgtgcattg tgatcgggaa tgaggtggtc aacttcgagt
     1561 ggacataccc ccgcaaagaa agtgggcggc tggtggagcc ggtgactgac ttcctcttgg
     1621 atatgcctta ccacatccgc tccatcctgc acatccccag tgccgagtta gaagactcgg
     1681 ggacctacac ctgcaatgtg acggagagtg tgaatgacca tcaggatgaa aaggccatca
     1741 acatcaccgt ggttgagagc ggctacgtgc ggctcctggg agaggtgggc acactacaat
     1801 ttgctgagct gcatcggagc cggacactgc aggtagtgtt cgaggcctac ccaccgccca
     1861 ctgtcctgtg gttcaaagac aaccgcaccc tgggcgactc cagcgctggc gaaatcgccc
     1921 tgtccacgcg caacgtgtcg gagacccggt atgtgtcaga gctgacactg gttcgcgtga
     1981 aggtggcaga ggctggccac tacaccatgc gggccttcca tgaggatgct gaggtccagc
     2041 tctccttcca gctacagatc aatgtccctg tccgagtgct ggagctaagt gagagccacc
     2101 ctgacagtgg ggaacagaca gtccgctgtc gtggccgggg catgccccag ccgaacatca
     2161 tctggtctgc ctgcagagac ctcaaaaggt gtccacgtga gctgccgccc acgctgctgg
     2221 ggaacagttc cgaagaggag agccagctgg agactaacgt gacgtactgg gaggaggagc
     2281 aggagtttga ggtggtgagc acactgcgtc tgcagcacgt ggatcggcca ctgtcggtgc
     2341 gctgcacgct gcgcaacgct gtgggccagg acacgcagga ggtcatcgtg gtgccacact
     2401 ccttgccctt taaggtggtg gtgatctcag ccatcctggc cctggtggtg ctcaccatca
     2461 tctcccttat catcctcatc atgctttggc agaaggagat ccgatggaag gtgattgagt
     2521 ctgtgagctc tgacggccat gagtacatct acgtggaccc catgcagctg ccctatgact
     2581 ccacgtggga gctgccgcgg gaccagcttg tgctgggacg caccctcggc tctggggcct
     2641 ttgggcaggt ggtggaggcc acggctcatg gcctgagcca ttctcaggcc acgatgaaag
     2701 tggccgtcaa gatgcttaaa tccacagccc gcagcagtga gaagcaagcc cttatgtcgg
     2761 agctgaagat catgagtcac cttgggcccc acctgaacgt ggtcaacctg ttgggggcct
     2821 gcaccaaagg aggacccatc tatatcatca ctgagtactg ccgctacgga gacctggtgg
     2881 actacctgca ccgcaacaaa cacaccttcc tgcagcacca ctccgacaag cgccgcccgc
     2941 ccagcgcgga gctctacagc aatgctctgc ccgttgggct ccccctgccc agccatgtgt
     3001 ccttgaccgg ggagagcgac ggtggctaca tggacatgag caaggacgag tcggtggact
     3061 atgtgcccat gctggacatg aaaggagacg tcaaatatgc agacatcgag tcctccaact
     3121 acatggcccc ttacgataac tacgttccct ctgcccctga gaggacctgc cgagcaactt
     3181 tgatcaacga gtctccagtg ctaagctaca tggacctcgt gggcttcagc taccaggtgg
     3241 ccaatggcat ggagtttctg gcctccaaga actgcgtcca cagagacctg gcggctagga
     3301 acgtgctcat ctgtgaaggc aagctggtca agatctgtga ctttggcctg gctcgagaca
     3361 tcatgcggga ctcgaattac atctccaaag gcagcacctt tttgccttta aagtggatgg
     3421 ctccggagag catcttcaac agcctctaca ccaccctgag cgacgtgtgg tccttcggga
     3481 tcctgctctg ggagatcttc accttgggtg gcacccctta cccagagctg cccatgaacg
     3541 agcagttcta caatgccatc aaacggggtt accgcatggc ccagcctgcc catgcctccg
     3601 acgagatcta tgagatcatg cagaagtgct gggaagagaa gtttgagatt cggcccccct
     3661 tctcccagct ggtgctgctt ctcgagagac tgttgggcga aggttacaaa aagaagtacc
     3721 agcaggtgga tgaggagttt ctgaggagtg accacccagc catccttcgg tcccaggccc
     3781 gcttgcctgg gttccatggc ctccgatctc ccctggacac cagctccgtc ctctatactg
     3841 ccgtgcagcc caatgagggt gacaacgact atatcatccc cctgcctgac cccaaacccg
     3901 aggttgctga cgagggccca ctggagggtt cccccagcct agccagctcc accctgaatg
     3961 aagtcaacac ctcctcaacc atctcctgtg acagccccct ggagccccag gacgaaccag
     4021 agccagagcc ccagcttgag ctccaggtgg agccggagcc agagctggaa cagttgccgg
     4081 attcggggtg ccctgcgcct cgggcggaag cagaggatag cttcctgtac ccttacgacg
     4141 tgccagacta cgcctagaga tctgcctcga ctgtgccttc tagttgccag ccatctgttg
     4201 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     4261 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     4321 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggact
     4381 cgagttaagg gcgaattccc gataaggatc ttcctagagc atggctacgt agataagtag
     4441 catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct
     4501 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     4561 gcccgggcgg cctcagtgag cgagcgagcg cgcagcctta attaacctaa ttcactggcc
     4621 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4681 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4741 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4801 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4861 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4921 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4981 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5041 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5101 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5161 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5221 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5281 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5341 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5401 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5461 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5521 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5581 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5641 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5701 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5761 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5821 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5881 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5941 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6001 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6061 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6121 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6181 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6241 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6301 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6361 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6421 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6481 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6541 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6601 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6661 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6721 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6781 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6841 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6901 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6961 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7021 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7081 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7141 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7201 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7261 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7321 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7381 atgattacgc cagatttaat taagg
//
