LOCUS       sequence-408086-        9315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, KAT6A_ADAM2 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_KAT6A_ADAM2
FEATURES             Location/Qualifiers
     source          1..9315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1449..1468)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1514..1569
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1568..1587)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1930..1946)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1930..1946)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1943..1965)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1954..1970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1978..1984,1985..2002,2003..2008))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2023..2044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2103..2298
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2149..2284
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2304..2438
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2341..2360)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2395..2414
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2506..2523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2677..3265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2757..2776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3436..4227,4228..4296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4059..4078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4297..4401)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4432..4811
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             4813..5070
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5117..5242
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5734..5851
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6211..6444
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6629..6673
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        6930..7233
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7234..7437
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7388..7408
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      7513..7522
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7519..7521,7522..7524,7525..8235)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7564..7585)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7825..7844)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8172..8193
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 attatgaacc cggaagcaat ccacttttca ggggtgaaaa ttttttctaa ctgttctttc
       61 gaagattttg cacattttat tagtaagcag aagtcccagt gcttgcataa tcaaccacgc
      121 cttgacccct tttttaagca gcaggcagta tgtggtaatg ccaaattgga ggccggcgaa
      181 gaatgcgact gcgggacaga acaagattgt gctttgattg gtgagacttg ttgcgatatt
      241 gcaacctgtc gcttcaaagc tgggtcaaat tgtgccgagg gtccgtgttg tgagaactgc
      301 cttttcatga gcaaagagcg catgtgccgc ccgagttttg aggagtgcga tcttcctgag
      361 tattgcaacg gttcttcggc gagttgccct gaaaatcact acgttcagac ggggcaccca
      421 tgtggtttaa accaatggat ttgtatcgac ggcgtctgca tgtcaggaga taaacagtgt
      481 actgacacct ttggcaaaga ggtcgagttc gggcctagcg agtgttactc ccatttaaac
      541 agcaagacag acgtgagtgg aaactgcggg atctccgaca gcgggtatac gcagtgtgag
      601 gccgacaatc tgcaatgtgg aaaacttatc tgtaagtacg taggaaaatt tttactgcaa
      661 atcccacgcg ctacaattat ctacgctaat atttctggtc acctttgcat cgcagtagaa
      721 tttgcgtcgg atcatgctga ctctcagaag atgtggatta aagatgggac gagctgcggg
      781 tctaataagg tgtgtcgtaa tcagcgttgc gtttcaagta gttatttagg ttacgactgt
      841 accaccgaca agtgcaacga tcgtggtgtg tgcaataaca agaagcactg ccattgttca
      901 gccagttacc tgcctccaga ttgttccgta cagagtgatt tatggccagg ggggtctatt
      961 gatagtggga attttccacc ggtcgcgatc cccgcccgtc tgccagagcg ccgctatatc
     1021 gagaatatct accacagtaa acccatgcgc tggccatttt ttctgttcat cccgttcttc
     1081 atcatcttct gcgtactgat cgccatcatg gtgaaagtta acttccagcg taaaaagtgg
     1141 cgcaccgaag actattcaag cgacgagcag cccgaaagtg agagcgaacc gaaggggtga
     1201 accggttcta gataagatta cggtaccttt aagaccaatg acttacaagg cagctgtaga
     1261 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaaagaag
     1321 acaagatctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     1381 gctctctggc taactaggga acccactgct taagcctcaa taaagcttca gctgctcgag
     1441 ctagcagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     1501 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     1561 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     1621 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     1681 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     1741 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     1801 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     1861 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc
     1921 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1981 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     2041 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
     2101 ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     2161 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     2221 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     2281 ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa
     2341 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2401 tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca
     2461 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2521 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2581 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2641 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2701 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2761 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2821 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2881 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2941 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3001 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3061 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3121 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3181 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3241 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3301 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3361 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3421 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3481 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3541 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3601 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3661 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3721 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3781 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3841 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3901 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3961 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4021 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     4081 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4141 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4201 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4261 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4321 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4381 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgacattgat
     4441 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4501 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4561 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4621 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4681 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4741 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4801 ctattaccat gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     4861 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     4921 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     4981 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5041 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     5101 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     5161 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     5221 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     5281 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     5341 cagggagcta gaacgattcg cagttaatac tggcctgtta gaaacatcag aaggctgtag
     5401 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     5461 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     5521 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaaaaaag cacagcaagc
     5581 agcaggatct tcagacctgg aaattcccta caatccccaa agtcaaggag tagtagaatc
     5641 tatgaataaa gaattaaaga aaattatagg acaggtaaga gatcaggctg aacatcttaa
     5701 gacagcagta caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     5761 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     5821 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagaaatcc
     5881 actttggaaa ggaccagcaa agctcctctg gaaaggtgaa ggggcagtag taatacaaga
     5941 taatagtgac ataaaagtag tgccaagaag aaaagcaaag atcattaggg attatggaaa
     6001 acagatggca ggtgatgatt gtgtggcaag tagacaggat gaggattaga acatggaaaa
     6061 gtttagtaaa acaccataag gaggagatat gagggacaat tggagaagtg aattatataa
     6121 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     6181 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     6241 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     6301 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     6361 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     6421 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     6481 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     6541 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     6601 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     6661 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     6721 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     6781 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     6841 gggaccgagc tcaagcttcg aacgcgtgct agttattaat agtaatcaat tacggggtca
     6901 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     6961 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     7021 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     7081 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     7141 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     7201 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     7261 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     7321 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     7381 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     7441 ttagtgaacc gggctcgttt agtgaaccga attctgcagt cgacggtacc gcgggcccgg
     7501 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     7561 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     7621 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     7681 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     7741 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     7801 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     7861 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     7921 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     7981 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     8041 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     8101 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
     8161 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     8221 gacgagctgt acaagggcgg aagcggaggc tccgggggaa gtgcggccgc ttcccggatg
     8281 gtgaagttgg ccaacccttt gtacacagaa tggattttag aggcaattaa aaaggtaaag
     8341 aagcaaaaac agcgtccctc cgaggaacgc atttgtaatg cagtaagctc ttcgcatggt
     8401 ttggaccgca aaaccgtcct ggaacaactg gaattgtctg ttaaggacgg aaccatcttg
     8461 aaagtctcga acaaaggttt gaattcctat aaagaccctg ataacccggg ccgtattgcc
     8521 cttcctaaac ctcgcaacca tggtaagtta gacaataagc aaaacgtaga ttggaacaag
     8581 ctgattaagc gcgcagtcga aggtttagca gaatccgggg gttctacatt gaagtcaatc
     8641 gaacgctttt tgaaggggca gaaggatgtt tccgccttat ttggtggttc cgctgcatca
     8701 ggtttccatc aacaattgcg tttagctatt aaacgcgcaa tcggacacgg tcgtttactt
     8761 aaagatggcc ccctttatcg tttaaacacg aaagcaacga atgttgatgg caaagagtca
     8821 tgcgagtcct taagctgcct tccccccgtt tcccttttgc cacatgagaa ggataaatac
     8881 aatcacatgg gttccgacac cactgtggtg gcacagaaag tattccaatt aatcgggtta
     8941 acgaatgcaa tcttcgtgag cttcaatatc acaattattc tttcatcctt agagttatgg
     9001 attgatgaga ataagatcgc aactactgga gaagcaaacg agttgttaca tacattcctt
     9061 cgctggaaga cttcgtacct ggttttgcgc ccgcacgatg ttgctttttt gctggtatac
     9121 cgcgagaagt cgaactatgt aggtgccaca tttcaaggga agatgtgcga tgccaactat
     9181 gcaggtgggg tcgttttgca tccccgtacg atctcgcttg agtcgcttgc cgttattctg
     9241 gcacaactgt tgtctttatc catgggcatc acttatgacg acattaacaa gtgccaatgt
     9301 tccggcgctg tatgc
//
