LOCUS       sequence-408090-        8823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, JARID2_SYNCRIP from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_JARID2_SYNCRIP
FEATURES             Location/Qualifiers
     source          1..8823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3584..4294,4295..4297,4298..4300))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3626..3647)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3975..3994
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4234..4255
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4297..4306
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4382..4585)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4411..4431)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4586..4889
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5146..5190)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5375..5608
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5968..6085
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6577..6702
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6749..7006
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7008..7387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7418..7522
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7523..7591,7592..8383)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7741..7760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8554..8823,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcatt
     1801 gcaaaagatc ggggccggcc tcaacaccct tacctcctcg acccctttgt tgctgcgcac
     1861 cccccctagc tcctctcacc cctcgagctg acccaggacc gcctctttgt gagtacccag
     1921 cccgaccgcg aggtggagct gcgcccctac ctctactggg cgccgcccct cgagcgccgc
     1981 gtccaccgcg accgcgagcc ccaacttgaa aatcctcgta gccataatac gggtcttcgt
     2041 aaccgccgcg gtagttgtga taatcataac catagtagtc atagtagtcc tcatacccgt
     2101 aatagtcggg ggggtacccg taaccccccc gccctcctcg tccgcgaccc ctggtaggag
     2161 gcggcatgtg gggcggacca taataatagt aatcgtcata catttgattt ttcgcggctt
     2221 gtcgctgcgc ttttctctcc tttcgcttct gatcaggggg cttggcgaac acgatttcga
     2281 tattctcgcc ttccagatct tttccgttca tctcttccat tgcttttact gcgccgtcgc
     2341 gctcgtcaaa gtgtataaag gcataatctt tcaacttttt tacccgctcc agcttcccga
     2401 actgagagaa ggccttttcg aggatttctt cggtaacggt attggcaaga ttccgtacaa
     2461 aaagcacttt gaccttagcc attacttcag gatctggatc ttcaatgggg tcggcccatt
     2521 caaccgtccc aacgttaccc cagactttca ctttgccgct catgaggcgg cgcctagctt
     2581 gtgccgccgt cttatggtct tcatactcca agaagcagaa gcccctgttt ttctttttat
     2641 cgtcgggttg atgatagagg ataacatcag taagtccctc cgttacttta ctgaactctt
     2701 ccaatatttg ctctttagtc ttgctcttgg gaatagaccc tacaaacaat cgattatttg
     2761 cgacggagat acacacacct atatgctttc cagatcttat ctcgtgatta ttatacaatt
     2821 tcacagcttc ctgtgccgcc tccttagtgc agaatgtaac aaaagcatac cccctgttga
     2881 ggccagtcaa cgggtccatc ataagtctga gatcccaaat aggccctgcc ttttcgaaca
     2941 ggggcacgag ttcgtcttcg aacaggtccc taggaatttt acccacaaag atctccgtac
     3001 ctacagaagg ctgttgtccg ctatacacag agtccggcgg tgggccgccg tattttctct
     3061 gacctgtggt gacatccaat gtataacccg ttctttccaa cagcgcctta atttttgcct
     3121 catccggtcc cttactactg tcggcgactt tagtgccttg tttttcgcgt tggcggtatg
     3181 ttttcatgac tccacacaga aacgcacttt tgttctgtac atgactcaag tcgctgtctt
     3241 tgaactgttg gagcaccgcc aaagcaccat cctcattaaa ctctttaaga gcctcaatgg
     3301 cccgctcgtc caggtcactg tgggccacaa gtcccgcgac gtatatttcg tcaagtttct
     3361 cggccacctt ctgtggcaaa ccggcatcaa ggagtgtttg gaaattctct gaatggatta
     3421 cggcactagt ggtatccatg ggttcttcag taccattccc gttaacgtgc tcggttgcca
     3481 tattgccgct aatatacttt ttctggataa tgtttcgttt tgggcgttcc ttagacattc
     3541 cccgggaagc ggccgcactt cccccggagc ctccgcttcc gcccttgtac agctcgtcca
     3601 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     3661 cgttggggtc tttgctcagc ttggactggg tgctcaggta gtggttgtcg ggcagcagca
     3721 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     3781 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
     3841 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     3901 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     3961 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     4021 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     4081 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
     4141 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     4201 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     4261 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc ggtggatccc
     4321 gggcccgcgg taccgtcgac tgcagaattc ggttcactaa acgagcccgg ttcactaaac
     4381 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     4441 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     4501 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     4561 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     4621 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     4681 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     4741 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     4801 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     4861 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     4921 accccgtaat tgattactat taataactag cacgcgttcg aagcttgagc tcggtcccct
     4981 cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac
     5041 tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa
     5101 taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt
     5161 atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa
     5221 ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg
     5281 attccaaatc tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt
     5341 gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct
     5401 ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag
     5461 atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa
     5521 taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc
     5581 tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac
     5641 tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt
     5701 atataattca cttctccaat tgtccctcat atctcctcct tatggtgttt tactaaactt
     5761 ttccatgttc taatcctcat cctgtctact tgccacacaa tcatcacctg ccatctgttt
     5821 tccataatcc ctaatgatct ttgcttttct tcttggcact acttttatgt cactattatc
     5881 ttgtattact actgcccctt cacctttcca gaggagcttt gctggtcctt tccaaagtgg
     5941 atttctgctg tccctgtaat aaacccgaaa attttgaatt tttgtaattt gtttttgtaa
     6001 ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg cactgtaccc
     6061 cccaatcccc ccttttcttt taaaattgtg gatgaatact gccatttgta ctgctgtctt
     6121 aagatgttca gcctgatctc ttacctgtcc tataattttc tttaattctt tattcataga
     6181 ttctactact ccttgacttt ggggattgta gggaatttcc aggtctgaag atcctgctgc
     6241 ttgctgtgct tttttcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt
     6301 ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa
     6361 tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct
     6421 acagccttct gatgtttcta acaggccagt attaactgcg aatcgttcta gctccctgct
     6481 tgcccatact atatgtttta atttatattt tttctttccc cctggcctta accgaatttt
     6541 ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc
     6601 cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct
     6661 tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt
     6721 cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt
     6781 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
     6841 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
     6901 gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg cttatatgca
     6961 ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctcccat ggtaatagcg
     7021 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
     7081 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
     7141 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
     7201 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
     7261 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
     7321 atatatgggc tatgaactaa tgaccccgta attgattact attaataact agtcaataat
     7381 caatgtcgac ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7441 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7501 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7561 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7621 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7681 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7741 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     7801 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7861 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7921 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7981 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8041 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8101 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8161 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8221 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8281 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8341 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8401 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8461 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8521 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8581 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8641 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8701 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8761 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8821 cgg
//
