LOCUS       sequence-408097-       10058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, KDM2A_C11orf80 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 10058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_KDM2A_C11orf80
FEATURES             Location/Qualifiers
     source          1..10058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4819..5529,5530..5532,5533..5535))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4861..4882)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5210..5229
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5469..5490
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5532..5541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5617..5820)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5646..5666)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5821..6124
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6381..6425)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6610..6843
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7203..7320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7812..7937
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7984..8241
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8243..8622
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8653..8757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8758..8826,8827..9618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8976..8995)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9789..10058,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcatg
     1801 gtcctggaga gggcgaaagc cactcactaa gattcgaaac ctcttgcaac cacagaacgt
     1861 cttcaaggcg tgatcccagg ctcgcgcggc caggcgctaa agcgcgaccg tgcgcctcgc
     1921 gatgttcacg tccacgtgga gttaacggag ccgctgtcgc ttcagggcga cgaggagcag
     1981 cttcacttgg tgatggggca cgagcggagc gtaatgcacg catttcctca atacggggac
     2041 tgccgcgctt cagacgctta ttttctgcct gaccggaagt gtctgctaac agctccaaag
     2101 agctattatc cggagcgtcc tcagttccct gtgctgaatc cttcttctca agagtcagct
     2161 gctgcttaac ttcgcacgag cagtgatgca agaattgatg ctgggtgatc tcacggaata
     2221 ctttgtgtaa ttttgtacga aactcctgag tatccgcggc ttgaagggtc tgtaaacaca
     2281 ttttacggaa ggacgagcgg gtactacctg ttaaaataga cataatcgag ttgactgcta
     2341 cgctcaatga tgcttgcagc ttctgttggg ccttcgcagc ttggtgatgc tgttcaagga
     2401 ccttatcaac tgtaaattga attgagcctt ggactactga gtgtgactct accaaaatag
     2461 cattcaagtg agttgtcaaa agtgtataca cgccccagat ttccatttgt tgaacaggaa
     2521 acgcagagtg gaagtcaacg aacaagaaca ataacagggt ctgcccttgg gggtccatag
     2581 tttgcgactg atcttcttct gacgattcca cttgataaga aacatctggc aagactaaat
     2641 cgcggtccag gtttaagtcc agattaggga tcatgcaaag atgatatttt ttccaatcaa
     2701 ccaaagatga tgtgtccttg aaaaacgtgg tcataggctg ttcccacgta gataaaataa
     2761 ggggaagccc caagggtcca tataaaaaga tagacacgct actaatacga ttaagttgat
     2821 tcgaggatac tttcggtgag ggacacaaag ccgccgctgg cgtaaggatt aattctccaa
     2881 gtaagtccat gccggctacg tcctcgggaa taaaaagcat taccgggtgt cctaatactg
     2941 gatgaatgcg actacaatgg gactcaatgg tgccaaaatt aggacgaacg tagtgttgcg
     3001 agtccaccag cagggcgata ccgttgccta aattcaaagt aggttcattt tcgaccccaa
     3061 agatctcggt cgataagatt ccattaactt tcacagagaa gtggaaacga accttcgggt
     3121 gaaccatgat aattttgcgc aaaaaatgct tgatgactaa acagtctgtc atcaatgtac
     3181 gtggcttttc gtctactaaa cgctgagagt aacgaatgcg ttcctcctct ggctccattc
     3241 cccgggaagc ggccgccagt gtgatggata tctgcagaat tcgcccttgt cattggtctt
     3301 aaaggtaccg taatcttatc tagaaccggt tcatggtcct ggagagggcg aaagccactc
     3361 actaagattc gaaacctctt gcaaccacag aacgtcttca aggcgtgatc ccaggctcgc
     3421 gcggccaggc gctaaagcgc gaccgtgcgc ctcgcgatgt tcacgtccac gtggagttaa
     3481 cggagccgct gtcgcttcag ggcgacgagg agcagcttca cttggtgatg gggcacgagc
     3541 ggagcgtaat gcacgcattt cctcaatacg gggactgccg cgcttcagac gcttattttc
     3601 tgcctgaccg gaagtgtctg ctaacagctc caaagagcta ttatccggag cgtcctcagt
     3661 tccctgtgct gaatccttct tctcaagagt cagctgctgc ttaacttcgc acgagcagtg
     3721 atgcaagaat tgatgctggg tgatctcacg gaatactttg tgtaattttg tacgaaactc
     3781 ctgagtatcc gcggcttgaa gggtctgtaa acacatttta cggaaggacg agcgggtact
     3841 acctgttaaa atagacataa tcgagttgac tgctacgctc aatgatgctt gcagcttctg
     3901 ttgggccttc gcagcttggt gatgctgttc aaggacctta tcaactgtaa attgaattga
     3961 gccttggact actgagtgtg actctaccaa aatagcattc aagtgagttg tcaaaagtgt
     4021 atacacgccc cagatttcca tttgttgaac aggaaacgca gagtggaagt caacgaacaa
     4081 gaacaataac agggtctgcc cttgggggtc catagtttgc gactgatctt cttctgacga
     4141 ttccacttga taagaaacat ctggcaagac taaatcgcgg tccaggttta agtccagatt
     4201 agggatcatg caaagatgat attttttcca atcaaccaaa gatgatgtgt ccttgaaaaa
     4261 cgtggtcata ggctgttccc acgtagataa aataagggga agccccaagg gtccatataa
     4321 aaagatagac acgctactaa tacgattaag ttgattcgag gatactttcg gtgagggaca
     4381 caaagccgcc gctggcgtaa ggattaattc tccaagtaag tccatgccgg ctacgtcctc
     4441 gggaataaaa agcattaccg ggtgtcctaa tactggatga atgcgactac aatgggactc
     4501 aatggtgcca aaattaggac gaacgtagtg ttgcgagtcc accagcaggg cgataccgtt
     4561 gcctaaattc aaagtaggtt cattttcgac cccaaagatc tcggtcgata agattccatt
     4621 aactttcaca gagaagtgga aacgaacctt cgggtgaacc atgataattt tgcgcaaaaa
     4681 atgcttgatg actaaacagt ctgtcatcaa tgtacgtggc ttttcgtcta ctaaacgctg
     4741 agagtaacga atgcgttcct cctctggctc cattccccgg gaagcggccg cacttccccc
     4801 ggagcctccg cttccgccct tgtacagctc gtccatgccg agagtgatcc cggcggcggt
     4861 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga
     4921 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
     4981 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa
     5041 gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta
     5101 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
     5161 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
     5221 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
     5281 gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt
     5341 cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
     5401 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
     5461 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
     5521 gcccttgctc accatggtgg cgaccggtgg atcccgggcc cgcggtaccg tcgactgcag
     5581 aattcggttc actaaacgag cccggttcac taaacgagct ctgcttatat agacctccca
     5641 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     5701 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     5761 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     5821 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     5881 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     5941 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     6001 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     6061 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     6121 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     6181 actagcacgc gttcgaagct tgagctcggt cccctcgggg ttgggaggtg ggtctgaaac
     6241 gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt
     6301 cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt
     6361 gttatgttaa accaattcca caaacttgcc catttatcta attccaataa ttcttgttca
     6421 ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg
     6481 ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta
     6541 ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc
     6601 caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg
     6661 agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc
     6721 agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc
     6781 gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct
     6841 cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct
     6901 actcctaatg gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc
     6961 ctcatatctc ctccttatgg tgttttacta aacttttcca tgttctaatc ctcatcctgt
     7021 ctacttgcca cacaatcatc acctgccatc tgttttccat aatccctaat gatctttgct
     7081 tttcttcttg gcactacttt tatgtcacta ttatcttgta ttactactgc cccttcacct
     7141 ttccagagga gctttgctgg tcctttccaa agtggatttc tgctgtccct gtaataaacc
     7201 cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct
     7261 attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa
     7321 ttgtggatga atactgccat ttgtactgct gtcttaagat gttcagcctg atctcttacc
     7381 tgtcctataa ttttctttaa ttctttattc atagattcta ctactccttg actttgggga
     7441 ttgtagggaa tttccaggtc tgaagatcct gctgcttgct gtgctttttt cttacttttg
     7501 ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga
     7561 tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc
     7621 tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg
     7681 ccagtattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta
     7741 tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc
     7801 cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt
     7861 tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga
     7921 gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc
     7981 cactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     8041 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     8101 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     8161 acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg ccactcccca
     8221 gtcccgccca ggccacgcct cccatggtaa tagcgatgac taatacgtag atgtactgcc
     8281 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     8341 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     8401 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     8461 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     8521 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     8581 ccgtaattga ttactattaa taactagtca ataatcaatg tcgacccagg tggcactttt
     8641 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8701 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8761 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8821 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8881 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8941 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     9001 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     9061 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     9121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     9181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     9241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     9301 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9481 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     9541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     9601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     9661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     9781 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     9841 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9901 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     9961 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    10021 gtggctgctg ccagtggcga taagtcgtgt cttaccgg
//
