LOCUS       sequence-408109-        7973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CcdB Dropout vector for part 4 of Hemagglutinin surface expression 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7973)
  TITLE     Human expression plasmids for Hemagglutinin surface expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HA Display H3_A/Hong Kong/1/1968 Part 4-DO
FEATURES             Location/Qualifiers
     source          1..7973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(115..134)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      928..1516
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1417..1436
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1670..1687
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1804..1825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1840..1845,1846..1863,1864..1870)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1878..1894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1883..1905
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1902..1918
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1902..1918
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1967..2088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1978..1997)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2032..2051
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2218..3240)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             complement(2218..3240)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(3248..3295)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     3427..3446
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3579..3803
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3792..3809
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3856..4002)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(4045..4068)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(4075..4098)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(4105..4170)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4433..4738
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4617..4636
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(6047..6073)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(6053..6079)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(6053..6079)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     sig_peptide     complement(6422..6481)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     promoter        complement(6527..6545)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6588..6607)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          6590..6722
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     complement(6841..6865)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6875..6893
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6896..6914
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(6916..7119)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6945..6965)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7120..7499
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7671..7690
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7766..7869
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7870..7938,7939..7973,1..757)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTHLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
       61 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      121 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      181 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      301 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      361 tcatgtaact caccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      421 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      481 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      541 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      601 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      661 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      721 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
      781 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      841 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      901 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      961 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1021 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1081 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1141 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1201 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1261 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1321 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1381 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1441 tcctgtcggg tttcgccacc tccgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1501 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1561 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1621 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1681 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     1741 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     1801 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     1861 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     1921 tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag atacattgat
     1981 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2041 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgaa
     2101 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
     2161 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgta gtacgtgcta
     2221 ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
     2281 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
     2341 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
     2401 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
     2461 ccgcggcgat cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat
     2521 acaagccaac cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa
     2581 catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt
     2641 ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat
     2701 cagctcatcg agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca
     2761 gtgatacaca tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc
     2821 gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat
     2881 cgcatccatg gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc
     2941 ttgcaacgtg acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc
     3001 cccaatgtca agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata
     3061 acgatctttg tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc
     3121 tacatcgaag ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac
     3181 gctgtcgaac ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt
     3241 ggccaaggaa gttcctatac tttctagaga ataggaactt cggaatagga acttctaggt
     3301 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3361 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3421 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3481 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtggggata
     3541 ccccctagag ccccagctgg ttctttccgc ctcagaagcc atagagccca ccgcatcccc
     3601 agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc
     3661 cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag
     3721 gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga
     3781 tggctggcaa ctagaaggca cagtcgaggc tgatcagcgg gtttaaacgg gccctctaga
     3841 ctcgagcggc cgttaacgtg gcttcttctg ccaaagcatg atgaggatga taagggagat
     3901 gatggtgagc accaccaggg ccaggatggc tgagatcacc accaccttaa agggcaagga
     3961 gtgtggcacc acgatgacct cctgcgtgtc ctggcccaca gcagctgagc cgccagatcc
     4021 gccccctcca ctaccgccgc cgcctgggcc ctggaacagc acctccaggg atcccttctc
     4081 gaactggggg tgggaccagg cgctcttgtc atcgtcatcc ttgtaatcga tgtcatgatc
     4141 tttataatca ccgtcatggt ctttgtagtc ggatctgccc agggtggcag gtgtcaaaaa
     4201 gaggtgtgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
     4261 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc
     4321 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
     4381 cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt
     4441 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     4501 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     4561 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     4621 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     4681 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     4741 gtcaggctcc cttatacaca gccagtctgc aggtcgatac agtagaaatt acagaaactt
     4801 tatcacgttt agtaagtata gaggctgaaa atccagatga agccgaacga cttgtaagag
     4861 aaaagtataa gagttgtgaa attgttcttg atgcagatga ttttcaggac tatgacacta
     4921 gcgtatatga ataggtagat gtttttattt tgtcacacaa aaaagaggct cgcacctctt
     4981 tttcttattt ctttttatga tttaatacgg cattgaggac aatagcgagt aggctggata
     5041 cgacgattcc gtttgagaag aacatttgga aggctgtcgg tcgactccac ctgccaccgt
     5101 tgtagctcca caggtcgatc ttggtgtcct ccacgtactt ctccaggtcc tggatccggc
     5161 cctccacctc gctgaactcc ttctcgatct ggtggaactt ctcgttggtc ttctcgatca
     5221 cccggttcag cttgccgttg atctggtcga tgccggcctg ggtgctcttc aggtcggcgg
     5281 cctggccggt gccctcgctg ttctggtgcc ggaagccgta ccagccgtcg atcatgccct
     5341 cccagccgtt ctcgatgaag ccggcgatgg cgccgaacag gccccgggtc tgcttctcgg
     5401 gcacgttccg catgccggtg gccagcttca gggtgttctg cttcacgtac ttggggcagg
     5461 cgccgtaggt gatcttgttc acgttctgga agggcttgtc gttggggatg ctgccgttgg
     5521 gggtgatgca ctcgctgata catgtatcga tgggggcgtc gctccgcatg atgctgctct
     5581 tgccggtccg catcttgaag tagccccggg gggcgatcag gttgccgttg ctgttgatca
     5641 ccagcacgtc gccgggcttc acgatggtcc agtagatgct gatccggctg ctcaggcccc
     5701 gcacccaggg ccggctgccg atgttgggga tgatggtctg ctggctccgc cgggtgctca
     5761 cggtcacccg gccgctggcc tgcacgtaca ggctggtctg ctcctggttg gtgctggggt
     5821 ggtgcacgcc ccagatgtac agcttgtcga agttgtcgtt gttgggcatg gtcacgttca
     5881 gcacggggta ggtgctgccg ctcttggtca gccagttcag ccggctgaag aagccgctgc
     5941 cggggccccg cttgcaggcg ttgctgccgc cgttctgggt cacgccggtc caggtgaagc
     6001 cctcggtgat gaactccagg gtgccgctgc tggccaccag gctccgcagg ctggcgtagt
     6061 cgggcacgtc gtaggggtag cagttgctga aggccttgct ccgctccacg aacaggtccc
     6121 aggtctcgtt ctggaacacg tcgcagtggg ggtcgcccag cagggcgtcg atcagggtgc
     6181 agtcgatgcc gtccaggatc cggtgggggt tgttgcagat cttgccggtg ctgctgctct
     6241 gcaccagctc ggtggcgttg gtcacctcga tctggtcgtc ggtgatggtc ttcaccaggg
     6301 tgccgttggg cacggcgtgg tggcccaggc acagggtggc ggtgctgttg tcgttgccgg
     6361 gcaggtcctg gcccagggcc aggcagaaga tgtagctcag ggcgatgatg gtcttcatgt
     6421 caccagtgga acctggaacc cagagcagca gtacccatag caggagtgtg tctgtctcca
     6481 tcccgggtcg actctagagg taccacgcgt gaattctcga ggctagccta tagtgagtcg
     6541 tattaagtac tctagcctta agagctgtaa ttgaactggg agtggacacc tgtggagaga
     6601 aaggcaaagt ggatgtcagt aagaccaata ggtgcctatc agaaacgcaa gagtcttctc
     6661 tgtctcgaca agcccagttt ctattggtct ccttaaacct gtcttgtaac cttgatactt
     6721 acctgcccag tgcctcacga ccaacttctg cagcttaagt ttaaacgcta gagtccggag
     6781 gctggatcgg tcccggtgtc ttctatggag gtcaaaacag cgtggatggc gtctccaggc
     6841 gatctgacgg ttcactaaac gagctcgtcg acgatctcta tcactgatag ggagatctct
     6901 atcactgata gggagagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     6961 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     7021 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     7081 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     7141 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     7201 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     7261 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     7321 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     7381 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     7441 gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtca
     7501 acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt
     7561 cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc tcgcgcacta
     7621 ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg cggcatcaga
     7681 gcagattgta ctgagagtgc accatagggg atcgggagat ctcccgatcc gtcgacgtca
     7741 ggtggcactt ttcggggaaa tgtgtgcgga acccctattt gtttattttt ctaaatacat
     7801 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7861 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7921 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tga
//
