LOCUS       sequence-408112-        5998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5998)
  TITLE     Henry Paulson's unpublished plasmids
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFLAG-6a-Ataxin3Q80
FEATURES             Location/Qualifiers
     source          1..5998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     69..91
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     83..100
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     84..100
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        261..640
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        641..844
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     795..815
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             879..902
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2282..2904
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2481..2500)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2928..2948)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2933..3262
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3113..3248
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3300..3319)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3301..3319)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3333..3349)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3333..3349)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3346..3368)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3357..3373
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3381..3387,3388..3405,3406..3411))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3426..3447
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3564..3581)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3735..4323)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3815..3834)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4494..5285,5286..5354))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5117..5136
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5355..5459)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5486..5941
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5573..5592)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5783..5804
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
       61 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctga tctatacatt
      121 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
      181 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
      241 tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
      301 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      361 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      421 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      481 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
      541 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
      601 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      661 caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      721 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa
      781 ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
      841 agctcgttta gtgaaccgtc agaattacgc gtaccatgga ctacaaagac gatgacgaca
      901 agctcaagct tataaacgga gagtccatct tccacgagaa acaagaaggc tcactttgtg
      961 ctcaacattg cctgaataac ttattgcaag gagaatattt tagccctgtg gaattatcct
     1021 caattgcaca tcagctggat gaggaggaga ggatgagaat ggcagaagga ggagttacta
     1081 gtgaagatta tcgcacgttt ttacagcagc cttctggaaa tatggatgac agtggttttt
     1141 tctctattca ggttataagc aatgccttga aagtttgggg tttagaacta atcctgttca
     1201 acagtccaga gtatcagagg ctcaggatcg atcctataaa tgaaagatca tttatatgca
     1261 attataagga acactggttt acagttagaa aattaggaaa acagtggttt aacttgaatt
     1321 ctctcttgac gggtccagaa ttaatatcag atacatatct tgcacttttc ttggctcaat
     1381 tacaacagga aggttattct atatttgttg ttaagggtga tctgccagat tgcgaagctg
     1441 accaactcct gcagatgatt agggtccaac agatgcatcg accaaaactt attggagaag
     1501 aattagcaca actaaaagag caaggagtcc ataaaacaga cctggaacga atgttagaag
     1561 caaatgatgg ctcaggaatg ttagacgaag atgaggagga tttgcagagg gctctggcac
     1621 taagtcgcca agaaattgac atggaagatg aggaagcaga tctccgcagg gctattcagc
     1681 taagtatgca aggtagttcc agaaacatat ctcaagatat gacacagaca tcaggtacaa
     1741 atcttacttc agaagagctt cggaagagac gagaagccta ctttgaaaaa cagcagcaaa
     1801 agcagcaaca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
     1861 agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
     1921 agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcagcagc
     1981 agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcagcag cagcgggacc
     2041 tatcaggaca gagttcacat ccatgtgaaa ggccagccac cagttcagga gcacttggga
     2101 gtgatctagg tgatgctatg agtgaagaag acatgcttca ggcagctgtg accatgtctt
     2161 tagaaactgt cagaaatgat ttgaaaacag aaggaaaaaa ataaataaga atgcggccgc
     2221 gaattcagat ctctgcagga tatcgtcgac ggatccggta ccatcgatag ataattgacc
     2281 cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc
     2341 agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc
     2401 cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac
     2461 aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg
     2521 gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt
     2581 gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg
     2641 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc
     2701 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct
     2761 gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc tacctggcca
     2821 tgcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc
     2881 cactcagtag atgcctgttg aattgggtac gcggccagct tggctgtgga atgtgtgtca
     2941 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3001 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     3061 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     3121 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     3181 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     3241 tggaggccta ggcttttgca aaaagctcct cgaggaactg aaaaaccaga aagttaattc
     3301 cctatagtga gtcgtattaa attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
     3361 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3421 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3481 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3541 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3601 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3661 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3721 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3781 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3841 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3901 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3961 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4021 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4081 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4141 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4201 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4261 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4321 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4381 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4441 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4501 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4561 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4621 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4681 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4741 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4801 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4861 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4921 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4981 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     5041 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5101 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5161 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5221 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5281 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5341 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5401 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5461 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5521 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5581 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5641 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5701 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5761 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5821 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5881 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     5941 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttc
//
