LOCUS       sequence-408123-        8970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, MEF2D_HNRNPUL1 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 8970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_MEF2D_HNRNPUL1
FEATURES             Location/Qualifiers
     source          1..8970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3731..4441,4442..4444,4445..4447))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3773..3794)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4122..4141
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4381..4402
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4444..4453
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4529..4732)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4558..4578)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4733..5036
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5293..5337)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5522..5755
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6115..6232
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6724..6849
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6896..7153
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7155..7534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7565..7669
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7670..7738,7739..8530)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7888..7907)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8701..8970,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcact
     1801 gtgtgctcgt accaccctgc gtgttcccgc tataagatcc gtaatcgtag ttgccgtagt
     1861 atggcggcca ttgcccttgg ttttggtagt actgattcca ttgttgggcg tactgctgat
     1921 attgattgta tgagggttgc gggtaggtct gggcagtcgg gggcggggga ggcgtatagg
     1981 gcgctggatt ataaccgccg taggaaccgt aattgtatgc gggtggtggg ggcggaggtg
     2041 gcgggggagc ggtataacct tgagaatatc ctccttgatt atagggaggc tggctataag
     2101 aaggttgagg aggggaatag gatgaaacag ttggagtaga tgtattcgca cttgaaccag
     2161 gtatgttgct atttttattg tacgtactgg ctcctggtgg atttctcgcc ggggaatatg
     2221 agggtggcgg cgggggttgt tgcggtggcg gttgaggcgg gggaggctgt tgaggcgggg
     2281 gttgttgctg aggtgctcga ttgtaggacc ctcggttgtt tgaattgtta ttatccctgt
     2341 tgttattccc ccagcgattt tggctgtatc ctccaccccc actcctatta aaccctcccc
     2401 ggtaattccc gccgccgccg gaacctcccc ctcgattctg gaagccgccc cggttgccgc
     2461 caggcggacc tcgattctca tacctttgga accccccccc tccaccccta cctcggaaac
     2521 caccaccgcc tcggttgtca aaccgcttct ccggaggagg gccggccttt cgcccctctt
     2581 cgttgtactg ccggacgagc ttgtccgcct cctcccgttg gagctcaatg aacaaaacct
     2641 catccaagaa gtcccccacg tctggcaaag taaagttagc cgtgttataa gcggttggca
     2701 tggaagagaa tggcaaccct tgggacaaca aactaggggt tgcgacggac acaactggcg
     2761 tagtgagaga atgggtactc tggcttacac ccagtcgctg cgcattgtta agatccaagt
     2821 ggtcttcggt cagatgatgc atgagacctt taccagcctg tgaggttata actcggaggt
     2881 ccggttttcg tgatggtgcg ccgagctgtg tgctatgcgt cggtggtgga gggcttttag
     2941 cgggtattac tttattcaaa gagttgccat tagccacagg caacagaccg ggagaggcgc
     3001 gggcggagac atacccgttg ccaacggggg atggacaggc cccattcgca gagttgagat
     3061 cgcctccaag catggcaccg gcactagccg gtcgctgcgg aaggcccggt gagactgaat
     3121 tgcgttgaag ggccggctgt tgtggagaga gaagtcgagg gtcagtcagg gagctcgtga
     3181 ccagtgaagg ggtgaccaag gaaccgctag ggttggaaaa ttgcagacta gactggttgc
     3241 tcacaggaac cgttacgggc atagcaaagt taggggctgg aacagttgac ccgtatctcc
     3301 tgaaaagacc gtcgagttcc tcgctagccc tccgatactt gtcttcaagc aagggagact
     3361 gttcaagtga gtcttcacca tcgggttctg gggagtcaca cccattgaag ccctttttcc
     3421 tcaaagtctc aataatatcc gcattagtcc gggattcgtg cggttcatta tactcagtgt
     3481 acttcaaaag gactttatcc atgtcggttg aagcatattg aaagagttta tttgagtggt
     3541 tgaagattat aagcgcgatt tcgcaatcac acaagacgct caactcataa gctttcttca
     3601 tcaggccaaa cttcctcttc gtgaatgtta cctgccgatt gcgttcgtct gtaattctct
     3661 ggatttggat tttcttgcgg cccattcccc gggaagcggc cgcacttccc ccggagcctc
     3721 cgcttccgcc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     3781 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc
     3841 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     3901 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     3961 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     4021 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     4081 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     4141 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     4201 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     4261 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     4321 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     4381 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     4441 tcaccatggt ggcgaccggt ggatcccggg cccgcggtac cgtcgactgc agaattcggt
     4501 tcactaaacg agcccggttc actaaacgag ctctgcttat atagacctcc caccgtacac
     4561 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     4621 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     4681 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     4741 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     4801 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     4861 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     4921 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     4981 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     5041 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagcac
     5101 gcgttcgaag cttgagctcg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg
     5161 tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc
     5221 taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt
     5281 aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc
     5341 ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc
     5401 tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca
     5461 actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc
     5521 caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt
     5581 gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt
     5641 gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga
     5701 cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc
     5761 cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa
     5821 tggttcaatt tttactactt tatatttata taattcactt ctccaattgt ccctcatatc
     5881 tcctccttat ggtgttttac taaacttttc catgttctaa tcctcatcct gtctacttgc
     5941 cacacaatca tcacctgcca tctgttttcc ataatcccta atgatctttg cttttcttct
     6001 tggcactact tttatgtcac tattatcttg tattactact gccccttcac ctttccagag
     6061 gagctttgct ggtcctttcc aaagtggatt tctgctgtcc ctgtaataaa cccgaaaatt
     6121 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
     6181 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
     6241 gaatactgcc atttgtactg ctgtcttaag atgttcagcc tgatctctta cctgtcctat
     6301 aattttcttt aattctttat tcatagattc tactactcct tgactttggg gattgtaggg
     6361 aatttccagg tctgaagatc ctgctgcttg ctgtgctttt ttcttacttt tgttttgctc
     6421 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
     6481 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
     6541 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccagtatt
     6601 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
     6661 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
     6721 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
     6781 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
     6841 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
     6901 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     6961 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     7021 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     7081 caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc
     7141 caggccacgc ctcccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     7201 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     7261 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     7321 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     7381 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     7441 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     7501 gattactatt aataactagt caataatcaa tgtcgaccca ggtggcactt ttcggggaaa
     7561 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7621 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7681 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7741 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7801 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7861 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7921 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     7981 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8041 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8101 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8161 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8221 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8281 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8341 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8401 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8461 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8521 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8581 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8641 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8701 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8761 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8821 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8881 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8941 tgccagtggc gataagtcgt gtcttaccgg
//
