LOCUS       sequence-408125-        7417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses wild-type PDGFRb gene. Used for DNA delivery using 
            Adeno-associated Virus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Shima Y, Sasagawa S, Ota N, Oyama R, Tanaka M, Kubota-Sakashita M, 
            Kawakami H, Kobayashi M, Takubo N, Ozeki AN, Sun X, Kim YJ, Kamatani
            Y, Matsuda K, Maejima K, Fujita M, Noda K, Kamiyama H, Tanikawa R, 
            Nagane M, Shibahara J, Tanaka T, Rikitake Y, Mataga N, Takahashi S, 
            Kosaki K, Okano H, Furihata T, Nakaki R, Akimitsu N, Wada Y, Ohtsuka
            T, Kurihara H, Kamiguchi H, Okabe S, Nakafuku M, Kato T, Nakagawa H,
            Saito N, Nakatomi H
  TITLE     Increased PDGFRB and NF-kappaB signaling caused by highly prevalent 
            somatic mutations in intracranial aneurysms.
  JOURNAL   Sci Transl Med. 2023 Jun 14;15(700):eabq7721. doi: 
            10.1126/scitranslmed.abq7721. Epub 2023 Jun 14.
  PUBMED    37315111
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2023-06-14"; volume: "15(700):eabq7721. doi"; pages: "
            10.1126/scitranslmed.abq7721. Epub 2023 Jun 14"
COMMENT     Sequence Label: pAAV-CMV-PDGFRb-HA
FEATURES             Location/Qualifiers
     source          1..7417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     enhancer        271..574
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        575..778
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     729..749
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2347..2493
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             4129..4155
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4166..4183)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4172..4379
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4467..4596
                     /label=AAV2 ITR
     primer_bind     complement(4615..4632)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4615..4631)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4624..4646)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4773..5228
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4860..4879)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5070..5091
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5254..5358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5359..5427,5428..6219)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5577..5596)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6390..6978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6879..6898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7266..7287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7302..7307,7308..7325,7326..7332)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7340..7356
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7345..7367
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7364..7380
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7364..7380
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcgccc ttaagctagc tagttattaa tagtaatcaa ttacggggtc
      241 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      301 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      361 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      421 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      481 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      541 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      601 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      661 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      721 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
      781 tttagtgaac cgtcagatcc tgcagacacc atgcggcttc cgggtgcgat gccagctctg
      841 gccctcaaag gcgagctgct gttgctgtct ctcctgttac ttctggaacc acagatctct
      901 cagggcctgg tcgtcacacc cccggggcca gagcttgtcc tcaatgtctc cagcaccttc
      961 gttctgacct gctcgggttc agctccggtg gtgtgggaac ggatgtccca ggagccccca
     1021 caggaaatgg ccaaggccca ggatggcacc ttctccagcg tgctcacact gaccaacctc
     1081 actgggctag acacgggaga atacttttgc acccacaatg actcccgtgg actggagacc
     1141 gatgagcgga aacggctcta catctttgtg ccagatccca ccgtgggctt cctccctaat
     1201 gatgccgagg aactattcat ctttctcacg gaaataactg agatcaccat tccatgccga
     1261 gtaacagacc cacagctggt ggtgacactg cacgagaaga aaggggacgt tgcactgcct
     1321 gtcccctatg atcaccaacg tggcttttct ggtatctttg aggacagaag ctacatctgc
     1381 aaaaccacca ttggggacag ggaggtggat tctgatgcct actatgtcta cagactccag
     1441 gtgtcatcca tcaacgtctc tgtgaacgca gtgcagactg tggtccgcca gggtgagaac
     1501 atcaccctca tgtgcattgt gatcgggaat gaggtggtca acttcgagtg gacatacccc
     1561 cgcaaagaaa gtgggcggct ggtggagccg gtgactgact tcctcttgga tatgccttac
     1621 cacatccgct ccatcctgca catccccagt gccgagttag aagactcggg gacctacacc
     1681 tgcaatgtga cggagagtgt gaatgaccat caggatgaaa aggccatcaa catcaccgtg
     1741 gttgagagcg gctacgtgcg gctcctggga gaggtgggca cactacaatt tgctgagctg
     1801 catcggagcc ggacactgca ggtagtgttc gaggcctacc caccgcccac tgtcctgtgg
     1861 ttcaaagaca accgcaccct gggcgactcc agcgctggcg aaatcgccct gtccacgcgc
     1921 aacgtgtcgg agacccggta tgtgtcagag ctgacactgg ttcgcgtgaa ggtggcagag
     1981 gctggccact acaccatgcg ggccttccat gaggatgctg aggtccagct ctccttccag
     2041 ctacagatca atgtccctgt ccgagtgctg gagctaagtg agagccaccc tgacagtggg
     2101 gaacagacag tccgctgtcg tggccggggc atgccccagc cgaacatcat ctggtctgcc
     2161 tgcagagacc tcaaaaggtg tccacgtgag ctgccgccca cgctgctggg gaacagttcc
     2221 gaagaggaga gccagctgga gactaacgtg acgtactggg aggaggagca ggagtttgag
     2281 gtggtgagca cactgcgtct gcagcacgtg gatcggccac tgtcggtgcg ctgcacgctg
     2341 cgcaacgctg tgggccagga cacgcaggag gtcatcgtgg tgccacactc cttgcccttt
     2401 aaggtggtgg tgatctcagc catcctggcc ctggtggtgc tcaccatcat ctcccttatc
     2461 atcctcatca tgctttggca gaagaagcca cgttacgaga tccgatggaa ggtgattgag
     2521 tctgtgagct ctgacggcca tgagtacatc tacgtggacc ccatgcagct gccctatgac
     2581 tccacgtggg agctgccgcg ggaccagctt gtgctgggac gcaccctcgg ctctggggcc
     2641 tttgggcagg tggtggaggc cacggctcat ggcctgagcc attctcaggc cacgatgaaa
     2701 gtggccgtca agatgcttaa atccacagcc cgcagcagtg agaagcaagc ccttatgtcg
     2761 gagctgaaga tcatgagtca ccttgggccc cacctgaacg tggtcaacct gttgggggcc
     2821 tgcaccaaag gaggacccat ctatatcatc actgagtact gccgctacgg agacctggtg
     2881 gactacctgc accgcaacaa acacaccttc ctgcagcacc actccgacaa gcgccgcccg
     2941 cccagcgcgg agctctacag caatgctctg cccgttgggc tccccctgcc cagccatgtg
     3001 tccttgaccg gggagagcga cggtggctac atggacatga gcaaggacga gtcggtggac
     3061 tatgtgccca tgctggacat gaaaggagac gtcaaatatg cagacatcga gtcctccaac
     3121 tacatggccc cttacgataa ctacgttccc tctgcccctg agaggacctg ccgagcaact
     3181 ttgatcaacg agtctccagt gctaagctac atggacctcg tgggcttcag ctaccaggtg
     3241 gccaatggca tggagtttct ggcctccaag aactgcgtcc acagagacct ggcggctagg
     3301 aacgtgctca tctgtgaagg caagctggtc aagatctgtg actttggcct ggctcgagac
     3361 atcatgcggg actcgaatta catctccaaa ggcagcacct ttttgccttt aaagtggatg
     3421 gctccggaga gcatcttcaa cagcctctac accaccctga gcgacgtgtg gtccttcggg
     3481 atcctgctct gggagatctt caccttgggt ggcacccctt acccagagct gcccatgaac
     3541 gagcagttct acaatgccat caaacggggt taccgcatgg cccagcctgc ccatgcctcc
     3601 gacgagatct atgagatcat gcagaagtgc tgggaagaga agtttgagat tcggcccccc
     3661 ttctcccagc tggtgctgct tctcgagaga ctgttgggcg aaggttacaa aaagaagtac
     3721 cagcaggtgg atgaggagtt tctgaggagt gaccacccag ccatccttcg gtcccaggcc
     3781 cgcttgcctg ggttccatgg cctccgatct cccctggaca ccagctccgt cctctatact
     3841 gccgtgcagc ccaatgaggg tgacaacgac tatatcatcc ccctgcctga ccccaaaccc
     3901 gaggttgctg acgagggccc actggagggt tcccccagcc tagccagctc caccctgaat
     3961 gaagtcaaca cctcctcaac catctcctgt gacagccccc tggagcccca ggacgaacca
     4021 gagccagagc cccagcttga gctccaggtg gagccggagc cagagctgga acagttgccg
     4081 gattcggggt gccctgcgcc tcgggcggaa gcagaggata gcttcctgta cccttacgac
     4141 gtgccagact acgcctagag atctgcctcg actgtgcctt ctagttgcca gccatctgtt
     4201 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     4261 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     4321 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggac
     4381 tcgagttaag ggcgaattcc cgataaggat cttcctagag catggctacg tagataagta
     4441 gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc
     4501 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     4561 tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt aattaaccta attcactggc
     4621 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     4681 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     4741 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     4801 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4861 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4921 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4981 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5041 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5101 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5161 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     5221 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5281 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5341 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5401 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5461 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5521 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5581 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5641 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5701 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5761 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5821 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5881 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5941 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6001 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6061 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6121 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6181 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6241 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6301 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6361 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6421 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6481 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6901 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6961 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7021 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7081 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7141 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7201 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7261 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7321 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7381 catgattacg ccagatttaa ttaaggcctt aattagg
//
