LOCUS       sequence-408126-        9569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses wild-type PDGFRb gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9569)
  AUTHORS   Shima Y, Sasagawa S, Ota N, Oyama R, Tanaka M, Kubota-Sakashita M, 
            Kawakami H, Kobayashi M, Takubo N, Ozeki AN, Sun X, Kim YJ, Kamatani
            Y, Matsuda K, Maejima K, Fujita M, Noda K, Kamiyama H, Tanikawa R, 
            Nagane M, Shibahara J, Tanaka T, Rikitake Y, Mataga N, Takahashi S, 
            Kosaki K, Okano H, Furihata T, Nakaki R, Akimitsu N, Wada Y, Ohtsuka
            T, Kurihara H, Kamiguchi H, Okabe S, Nakafuku M, Kato T, Nakagawa H,
            Saito N, Nakatomi H
  TITLE     Increased PDGFRB and NF-kappaB signaling caused by highly prevalent 
            somatic mutations in intracranial aneurysms.
  JOURNAL   Sci Transl Med. 2023 Jun 14;15(700):eabq7721. doi: 
            10.1126/scitranslmed.abq7721. Epub 2023 Jun 14.
  PUBMED    37315111
REFERENCE   2  (bases 1 to 9569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2023-06-14"; volume: "15(700):eabq7721. doi"; pages: "
            10.1126/scitranslmed.abq7721. Epub 2023 Jun 14"
COMMENT     Sequence Label: pCAGGS-PDGFRb-IRES2-EGFP
FEATURES             Location/Qualifiers
     source          1..9569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..498
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        500..777
                     /label=chicken beta-actin promoter
     intron          778..1794
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1718..1740
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             3448..3594
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    join(5434..6008,6009..6011,6012..6020)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5601..5618)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5828..5847
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6021..6023,6024..6026,6027..6740)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6021..6023,6024..6026,6027..6737)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6066..6087)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6327..6346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6674..6695
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6770..6825
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6824..6843)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7186..7202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7186..7202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7199..7221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7210..7226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7234..7240,7241..7258,7259..7264))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7279..7300
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7359..7554
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7405..7540
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7560..7694
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7597..7616)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7651..7670
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7762..7779)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7933..8521)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8013..8032)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8692..9483,9484..9552))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9315..9334
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9553..9569,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      181 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      241 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      301 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      361 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      421 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      481 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
      541 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
      601 ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
      661 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      721 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      781 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      841 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      901 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      961 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
     1021 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
     1081 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1141 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1201 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1261 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1321 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1381 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1441 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1501 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1561 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1621 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1681 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1741 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1801 ggcaacgtgc tggttattgg gagagggcag taaggaggac ttcctggagg gggtgactgt
     1861 ccagagcctg gaactgtgcc cacaccagaa gccatcagca gcaaggacac catgcggctt
     1921 ccgggtgcga tgccagctct ggccctcaaa ggcgagctgc tgttgctgtc tctcctgtta
     1981 cttctggaac cacagatctc tcagggcctg gtcgtcacac ccccggggcc agagcttgtc
     2041 ctcaatgtct ccagcacctt cgttctgacc tgctcgggtt cagctccggt ggtgtgggaa
     2101 cggatgtccc aggagccccc acaggaaatg gccaaggccc aggatggcac cttctccagc
     2161 gtgctcacac tgaccaacct cactgggcta gacacgggag aatacttttg cacccacaat
     2221 gactcccgtg gactggagac cgatgagcgg aaacggctct acatctttgt gccagatccc
     2281 accgtgggct tcctccctaa tgatgccgag gaactattca tctttctcac ggaaataact
     2341 gagatcacca ttccatgccg agtaacagac ccacagctgg tggtgacact gcacgagaag
     2401 aaaggggacg ttgcactgcc tgtcccctat gatcaccaac gtggcttttc tggtatcttt
     2461 gaggacagaa gctacatctg caaaaccacc attggggaca gggaggtgga ttctgatgcc
     2521 tactatgtct acagactcca ggtgtcatcc atcaacgtct ctgtgaacgc agtgcagact
     2581 gtggtccgcc agggtgagaa catcaccctc atgtgcattg tgatcgggaa tgaggtggtc
     2641 aacttcgagt ggacataccc ccgcaaagaa agtgggcggc tggtggagcc ggtgactgac
     2701 ttcctcttgg atatgcctta ccacatccgc tccatcctgc acatccccag tgccgagtta
     2761 gaagactcgg ggacctacac ctgcaatgtg acggagagtg tgaatgacca tcaggatgaa
     2821 aaggccatca acatcaccgt ggttgagagc ggctacgtgc ggctcctggg agaggtgggc
     2881 acactacaat ttgctgagct gcatcggagc cggacactgc aggtagtgtt cgaggcctac
     2941 ccaccgccca ctgtcctgtg gttcaaagac aaccgcaccc tgggcgactc cagcgctggc
     3001 gaaatcgccc tgtccacgcg caacgtgtcg gagacccggt atgtgtcaga gctgacactg
     3061 gttcgcgtga aggtggcaga ggctggccac tacaccatgc gggccttcca tgaggatgct
     3121 gaggtccagc tctccttcca gctacagatc aatgtccctg tccgagtgct ggagctaagt
     3181 gagagccacc ctgacagtgg ggaacagaca gtccgctgtc gtggccgggg catgccccag
     3241 ccgaacatca tctggtctgc ctgcagagac ctcaaaaggt gtccacgtga gctgccgccc
     3301 acgctgctgg ggaacagttc cgaagaggag agccagctgg agactaacgt gacgtactgg
     3361 gaggaggagc aggagtttga ggtggtgagc acactgcgtc tgcagcacgt ggatcggcca
     3421 ctgtcggtgc gctgcacgct gcgcaacgct gtgggccagg acacgcagga ggtcatcgtg
     3481 gtgccacact ccttgccctt taaggtggtg gtgatctcag ccatcctggc cctggtggtg
     3541 ctcaccatca tctcccttat catcctcatc atgctttggc agaagaagcc acgttacgag
     3601 atccgatgga aggtgattga gtctgtgagc tctgacggcc atgagtacat ctacgtggac
     3661 cccatgcagc tgccctatga ctccacgtgg gagctgccgc gggaccagct tgtgctggga
     3721 cgcaccctcg gctctggggc ctttgggcag gtggtggagg ccacggctca tggcctgagc
     3781 cattctcagg ccacgatgaa agtggccgtc aagatgctta aatccacagc ccgcagcagt
     3841 gagaagcaag cccttatgtc ggagctgaag atcatgagtc accttgggcc ccacctgaac
     3901 gtggtcaacc tgttgggggc ctgcaccaaa ggaggaccca tctatatcat cactgagtac
     3961 tgccgctacg gagacctggt ggactacctg caccgcaaca aacacacctt cctgcagcac
     4021 cactccgaca agcgccgccc gcccagcgcg gagctctaca gcaatgctct gcccgttggg
     4081 ctccccctgc ccagccatgt gtccttgacc ggggagagcg acggtggcta catggacatg
     4141 agcaaggacg agtcggtgga ctatgtgccc atgctggaca tgaaaggaga cgtcaaatat
     4201 gcagacatcg agtcctccaa ctacatggcc ccttacgata actacgttcc ctctgcccct
     4261 gagaggacct gccgagcaac tttgatcaac gagtctccag tgctaagcta catggacctc
     4321 gtgggcttca gctaccaggt ggccaatggc atggagtttc tggcctccaa gaactgcgtc
     4381 cacagagacc tggcggctag gaacgtgctc atctgtgaag gcaagctggt caagatctgt
     4441 gactttggcc tggctcgaga catcatgcgg gactcgaatt acatctccaa aggcagcacc
     4501 tttttgcctt taaagtggat ggctccggag agcatcttca acagcctcta caccaccctg
     4561 agcgacgtgt ggtccttcgg gatcctgctc tgggagatct tcaccttggg tggcacccct
     4621 tacccagagc tgcccatgaa cgagcagttc tacaatgcca tcaaacgggg ttaccgcatg
     4681 gcccagcctg cccatgcctc cgacgagatc tatgagatca tgcagaagtg ctgggaagag
     4741 aagtttgaga ttcggccccc cttctcccag ctggtgctgc ttctcgagag actgttgggc
     4801 gaaggttaca aaaagaagta ccagcaggtg gatgaggagt ttctgaggag tgaccaccca
     4861 gccatccttc ggtcccaggc ccgcttgcct gggttccatg gcctccgatc tcccctggac
     4921 accagctccg tcctctatac tgccgtgcag cccaatgagg gtgacaacga ctatatcatc
     4981 cccctgcctg accccaaacc cgaggttgct gacgagggcc cactggaggg ttcccccagc
     5041 ctagccagct ccaccctgaa tgaagtcaac acctcctcaa ccatctcctg tgacagcccc
     5101 ctggagcccc aggacgaacc agagccagag ccccagcttg agctccaggt ggagccggag
     5161 ccagagctgg aacagttgcc ggattcgggg tgccctgcgc ctcgggcgga agcagaggat
     5221 agcttcctgt agggggctgg cccctaccct gccctgcctg aagctccccc cctgccagca
     5281 cccagcatct cctggcctgg cctgaccggg cttcctgtca gccaggctgc ccttatcagc
     5341 tgtccccttc tggaagcttt ctgctcctga cgtgttgtgc cccaaaccct ggggctggct
     5401 taggaggcaa gaatgatatc gaattgggat ccgcccctct ccctcccccc cccctaacgt
     5461 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     5521 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     5581 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     5641 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     5701 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     5761 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     5821 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     5881 ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg tttagtcgag
     5941 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     6001 atgataatat ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     6061 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     6121 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     6181 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     6241 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     6301 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     6361 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     6421 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     6481 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     6541 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     6601 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     6661 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     6721 tggacgagct gtacaagtaa agcggccgct gagcatctga cttctggcta ataaaggaaa
     6781 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     6841 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     6901 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
     6961 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     7021 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     7081 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     7141 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     7201 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7261 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7321 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc
     7381 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7441 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7501 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta
     7561 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7621 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7681 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7741 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7801 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7861 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7921 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7981 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8041 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8101 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8161 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8221 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8281 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8341 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8401 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8461 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8521 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8581 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8641 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8701 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8761 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8821 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8881 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8941 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9001 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9061 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9121 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9181 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9241 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9301 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9361 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9421 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9481 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9541 ttgaatactc atactcttcc tttttcaat
//
