LOCUS       sequence-408147-        9108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, NPM1_ALK from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_NPM1_ALK
FEATURES             Location/Qualifiers
     source          1..9108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     RBS             3321..3329
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(join(3869..4579,4580..4582,4583..4585))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3911..3932)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4260..4279
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4519..4540
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4582..4591
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4667..4870)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4696..4716)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4871..5174
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5431..5475)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5660..5893
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6253..6370
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6862..6987
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7034..7291
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7293..7672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7703..7807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7808..7876,7877..8668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8026..8045)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8839..9108,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagac tagggacccg
     1801 gttgattcat ggagttcttc gattttaaaa tggtgtcctc ataatgtccg gcacctggcg
     1861 cggtggccgc ctccaacgga agcccttgtt gctgatagcc ataattcacg ttgccgcaag
     1921 gaaagtgacg taagcgaaat aaaggaacct ctttcatgtt tgccgtaagg ctagacggct
     1981 caagcaaaag gcttgcgccg ggcagacgtc cagtggcgac atttggagga acggtgcaag
     2041 acccctctaa tcccaagtta ccgcgttcgt ggggctcctt cttcgcaatc ggattatttt
     2101 ttttcgtcgg cttttcagta aaccaagatc cataggtagg attccaaaga cttgtgggtt
     2161 tattgcgcga tccgtgtacg cggtgcaatt cggatggcgg atttgattga ctaaatgcca
     2221 tgttcacatg tccaccttcg actgctggtc cgcgcgggac acgcacactt acctcagcag
     2281 cggtaggctt cttcgctgct ttccctgacg aagtggtcgg taagggtggt ggcgcggctg
     2341 gactgcgttc ttcttcgcgt ttggcttgtt gagaaaccag caagggaggt actccctctg
     2401 gatctttggg acgcactggc accttttcct cttcctccac cagagggccg tactcgatgg
     2461 gtaaggcggt gttaattacg tccgggtcct gggtacagta ctcgatgcgt tccaggatga
     2521 tagcgaaatt tggacggtct tcgggttgat gctgccaaca ttgggtcata atgcggtaca
     2581 ctgggccagg acagttttta ggcgggtcca tgcggcctcc gctcgtgaca aattccagta
     2641 cctcctggtt actctttgac ggatatggca tataacctaa actgaaaatt tcccaaagca
     2701 ggaccccaaa agaccaggta tctgtcttgg atgtaaaaat gccctccata aaggcctcgg
     2761 gtggcatcca ttttacgggt aacatagcgc aaccgccctt acggtaataa gaggcgcgat
     2821 aaatgtcacg agccatgcca aagtctccga tctttgctac gcgccctgga cccgggcatg
     2881 tcaaaagaca gttacgcgcg gcgatgtcgc gatggataaa gtggttttcc tccaagtact
     2941 gacacccaca tgcaatatcg cgagcaacgt gaagaagatc cagcatagcc agtgatgacg
     3001 gctggcttgg gcgagggcgt gtttcgcgca gaaacgattt caagtctcct cctgccatta
     3061 attccaacaa aatgaaacgc ggaagagatt gcaacgatac tccgatacaa cgaacaatgt
     3121 tttggtgatt aaattttgag atgataagtg cctccatcaa aaagtccagc tcatcttgct
     3181 cagagcagac ttcaggcaaa gtcttcacag caacctgtaa aggactgggg tcgtttggca
     3241 tgccgctcac ctgcccctca tagacttcgc caaaagctcc gtgccccagt ccgcgaataa
     3301 gtgtgatatt tttgcgtgga acctccttaa gatcgctgat tgagctagtt ttgcctgcga
     3361 aacagtagtt gggattatag tctgtcatga ttgtactcgt gcgcagttta gacaacttat
     3421 actctggcga ctgaagttcc atttgcatcg cttgtaactc ttgatgctta cggcggtaga
     3481 ctacaagatg ctgaccggaa atatgcactg gtccgcttcc gcacttcaga cgcagcacaa
     3541 ccggcggtgt gatctcaaaa cccccaaggc tgaccgttgg ctgaaccgac atcttcaatg
     3601 tagctaacgt caccttaatg ggtgaccctt cataattcat cgcctccgct tccacgatgt
     3661 gaagttcgtc tttagcgcct gctcccaaac taacagtacg cagagacagt tgatgctcat
     3721 tttcatcgtt gtcaaccttg aaatggtagt ccttatctgc tttcagctca cagccaaaca
     3781 ggtagttttg cggacgtaac ggactcatgt ccatatccat actatcctcc atagcggccg
     3841 cacttccccc ggagcctccg cttccgccct tgtacagctc gtccatgccg agagtgatcc
     3901 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     3961 tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     4021 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     4081 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     4141 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     4201 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     4261 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     4321 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     4381 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     4441 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     4501 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     4561 acagctcctc gcccttgctc accatggtgg cgaccggtgg atcccgggcc cgcggtaccg
     4621 tcgactgcag aattcggttc actaaacgag cccggttcac taaacgagct ctgcttatat
     4681 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
     4741 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
     4801 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
     4861 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     4921 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     4981 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     5041 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     5101 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     5161 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     5221 actattaata actagcacgc gttcgaagct tgagctcggt cccctcgggg ttgggaggtg
     5281 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
     5341 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
     5401 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
     5461 ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct
     5521 tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc
     5581 cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc
     5641 agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat
     5701 tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc
     5761 aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg
     5821 taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag
     5881 gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt
     5941 ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata attcacttct
     6001 ccaattgtcc ctcatatctc ctccttatgg tgttttacta aacttttcca tgttctaatc
     6061 ctcatcctgt ctacttgcca cacaatcatc acctgccatc tgttttccat aatccctaat
     6121 gatctttgct tttcttcttg gcactacttt tatgtcacta ttatcttgta ttactactgc
     6181 cccttcacct ttccagagga gctttgctgg tcctttccaa agtggatttc tgctgtccct
     6241 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat
     6301 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt
     6361 tcttttaaaa ttgtggatga atactgccat ttgtactgct gtcttaagat gttcagcctg
     6421 atctcttacc tgtcctataa ttttctttaa ttctttattc atagattcta ctactccttg
     6481 actttgggga ttgtagggaa tttccaggtc tgaagatcct gctgcttgct gtgctttttt
     6541 cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc
     6601 tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc
     6661 tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt
     6721 ttctaacagg ccagtattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg
     6781 ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct
     6841 aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct
     6901 agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc
     6961 agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct
     7021 gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     7081 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     7141 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     7201 aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg
     7261 ccactcccca gtcccgccca ggccacgcct cccatggtaa tagcgatgac taatacgtag
     7321 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     7381 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     7441 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     7501 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     7561 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     7621 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcgacccagg
     7681 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7741 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7801 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7861 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7921 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7981 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8041 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8101 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8161 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8221 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8281 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8341 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8401 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8461 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8521 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8581 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8641 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8701 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8761 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8821 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8881 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8941 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9001 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9061 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgg
//
