LOCUS       sequence-408149-        9573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PAX3_FOXO1 from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PAX3_FOXO1
FEATURES             Location/Qualifiers
     source          1..9573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4334..5044,5045..5047,5048..5050))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4376..4397)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4725..4744
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4984..5005
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5047..5056
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5132..5335)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5161..5181)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5336..5639
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5896..5940)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6125..6358
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6718..6835
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7327..7452
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7499..7756
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7758..8137
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8168..8272
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8273..8341,8342..9133)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8491..8510)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9304..9573,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagac tacccagata
     1801 cccaagagtg ggttgtggtc ttaacggagt gtggaaacga ttgattcggc aatacattat
     1861 caaaattaaa atccagcgtg tcaccatcca taaggtcgtt acggataatg gattccatat
     1921 cacaatccag gcgctcgatg aacatcccat caagatcaga cggcaacttc tcttgatgta
     1981 ataaacccat acgtccatac ccgttgcaac tcgaaacgct tgaataccct cccagcgcac
     2041 tcatctgcat ggggtgtggc agcggtactt ggaccggggt ctttacctgg gtcaagcgat
     2101 tcattccaga agtatggggc attgttgaga cggtatgggg taacgggcga ccattgacag
     2161 cggatgtttg ttgcgcatga cccggatgag tgtgagaact cggattcatc attttattgt
     2221 gtgaagcctg actaccgtaa gttgacatca cagaattagg ccccatcatg acgttttgcc
     2281 ctaaaacacg actgtttggc tgtgcaactc caggatcaac gggagtcata atatcattgt
     2341 gagggggtga atccgaagtc aacaactctt tcaaaaggcc cggggcgcaa ttatattgtg
     2401 acatcccgcc gtatgaggat ttattgtctt gtaaggtctg gatgggcatc tgtggcaacg
     2461 gcgacattga actttgcccg tatgtgtatt tctgatagtt cggactcgga ctattaagag
     2521 aagtattagg cggagcaaaa gaataacagg gcgtctgctg catcatggtg cctggagacg
     2581 attgggttga tactgttaac gatgtggggg aagacaacaa attcagattg tccaataagt
     2641 tctccatatt ttccgggtta ctgatctctg acagtgaagg caaagtagag gccatcttgg
     2701 ctgcagaggg gggataaacc atagagtgaa cgtcgccttc gcccaaatcg tcttgttcag
     2761 tcatgattgg agacaaacgg ccgctgattg tgctggcatt tgaactagtg cgggggcgaa
     2821 atgtgctcca gttatcgaaa tcgtcattcg agtgacttcc tggactcgca ggccacttgc
     2881 tgaactggga cccgggagag tcgcctgcac cctcctgacc agactgcagg ctagcctttt
     2941 tcttagcggc acgagaacgg ctctttgcaa atttagagtt gttatccatt gaggcggcac
     3001 gacggcgagg ggacttacca ctcttgccac cttcaggatt aagcatccac cacgatgatt
     3061 ttcctgtacc ttcgttctgt acacgaataa atttactatg taagctcaaa ttatgacgaa
     3121 tgctattctg gggtgacagg ccgttaccaa tcgtgggatt catagggttt gagggaccag
     3181 aaggtggaac gaagctgtca gtgtagctgg aaaaaccatg gcgtgtgctg ggaagacagt
     3241 acgccgaact agagtctggg ttgctaggaa tcgtggattg atgaactgta gacggtggta
     3301 agggctgagg acgatgcaca gtcgaactgg gatcagatac cgcttggggg atgcttgtgg
     3361 gctgatatga cgtctcactc aattggtacg tgggcagagt gggcatcgcg gtcgggggga
     3421 aaccaccagg gatcaaatgg ttaaaagcca ttaattggtt cgcgcccgcc tgtttgcgcc
     3481 aacgagcacg gcgattagag aaccagactt ggacacgtgc ttcggttaac ttagcacgtt
     3541 gtgccagctc ttcacgcgtg taaatatcag ggtagtgggt gcgctcaaaa gcgcgctcta
     3601 attcctccaa ctgttcagct gtaaaagtcg tgcggctgcg gcgctgtttg cgctttaacg
     3661 gcagatcagg ttcgctgtca atatccgatc cctcgtcgga ctgcggagct gacgcacgct
     3721 ctgacaggat accgtcgata ctgtgcttag cttttttttc tgattcttca gcctccttac
     3781 gctctaaatc cgcctcttcc tcctcccctt tgccgaattt actgcgcaaa atgcggctga
     3841 tagaacttac actgggaacc gtattacggt cacagacagc gtccttaagc aacttatcac
     3901 gaatttccca actgaacatc ccagggtttt cacgcttata ttcctcaatt ttcttctcca
     3961 catccggggt ggtgaccttg ggcttgctac cgccaattgc acccgggcga atgcttccag
     4021 tctcctggta gcggcacaaa atcttactga cgcatccgtg gctcacgcgc aactgacggc
     4081 tgataacaca ggggcgaatg ccgtggtggg ccatctcgac gattttatga cggatgtggt
     4141 ttggcaaagg acgaccgtta atgaataccc ctcccagttg gttaacacga ccctggccca
     4201 agggtgtgct tacttccaac gggaacccgc tgcgagggta gttctggccc gggcccgggc
     4261 gcatcatacg cggcactgca ccagctaagg tggtcatagc ggccgcactt cccccggagc
     4321 ctccgcttcc gcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     4381 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg
     4441 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     4501 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
     4561 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
     4621 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     4681 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     4741 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     4801 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg
     4861 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     4921 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     4981 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     5041 tgctcaccat ggtggcgacc ggtggatccc gggcccgcgg taccgtcgac tgcagaattc
     5101 ggttcactaa acgagcccgg ttcactaaac gagctctgct tatatagacc tcccaccgta
     5161 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     5221 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     5281 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     5341 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     5401 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     5461 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     5521 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     5581 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     5641 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
     5701 cacgcgttcg aagcttgagc tcggtcccct cggggttggg aggtgggtct gaaacgataa
     5761 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
     5821 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
     5881 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
     5941 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
     6001 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
     6061 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
     6121 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
     6181 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
     6241 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
     6301 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
     6361 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
     6421 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
     6481 atctcctcct tatggtgttt tactaaactt ttccatgttc taatcctcat cctgtctact
     6541 tgccacacaa tcatcacctg ccatctgttt tccataatcc ctaatgatct ttgcttttct
     6601 tcttggcact acttttatgt cactattatc ttgtattact actgcccctt cacctttcca
     6661 gaggagcttt gctggtcctt tccaaagtgg atttctgctg tccctgtaat aaacccgaaa
     6721 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     6781 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
     6841 gatgaatact gccatttgta ctgctgtctt aagatgttca gcctgatctc ttacctgtcc
     6901 tataattttc tttaattctt tattcataga ttctactact ccttgacttt ggggattgta
     6961 gggaatttcc aggtctgaag atcctgctgc ttgctgtgct tttttcttac ttttgttttg
     7021 ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac
     7081 acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag
     7141 ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagt
     7201 attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt
     7261 tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt
     7321 aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg
     7381 cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct
     7441 gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg
     7501 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     7561 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     7621 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     7681 gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact ccccagtccc
     7741 gcccaggcca cgcctcccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     7801 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     7861 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     7921 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     7981 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     8041 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     8101 attgattact attaataact agtcaataat caatgtcgac ccaggtggca cttttcgggg
     8161 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8221 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8281 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8341 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8401 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8461 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8521 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8581 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8641 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8701 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8761 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8821 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8881 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8941 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9001 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9061 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9121 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9181 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9241 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9301 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9361 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9421 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9481 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9541 tgctgccagt ggcgataagt cgtgtcttac cgg
//
