LOCUS       sequence-408154-        7869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PLEKHA5_XRCC6BP1 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 7869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PLEKHA5_XRCC6BP1
FEATURES             Location/Qualifiers
     source          1..7869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(122..226)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        257..636
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             638..895
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    942..1067
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1559..1676
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2036..2269
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2454..2498
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2755..3058
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3059..3262
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3213..3233
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3338..3347
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3344..3346,3347..3349,3350..4060)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3389..3410)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3650..3669)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3997..4018
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5143..5162)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5208..5263
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5262..5281)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5624..5640)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5624..5640)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5637..5659)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5648..5664
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5672..5678,5679..5696,5697..5702))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5717..5738
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5797..5992
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5843..5978
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5998..6132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6035..6054)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6089..6108
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6200..6217)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6371..6959)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6451..6470)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7130..7869,1..52,53..121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7753..7772
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
       61 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
      121 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
      181 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
      241 aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg
      301 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      361 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      421 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      481 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      541 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      601 cagtacatct acgtattagt catcgctatt accatgggag gcgtggcctg ggcgggactg
      661 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
      721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatactggcc
     1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1381 aaagtaagaa aaaagcacag caagcagcag gatcttcaga cctggaaatt ccctacaatc
     1441 cccaaagtca aggagtagta gaatctatga ataaagaatt aaagaaaatt ataggacagg
     1501 taagagatca ggctgaacat cttaagacag cagtacaaat ggcagtattc atccacaatt
     1561 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1621 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     1681 tttattacag ggacagcaga aatccacttt ggaaaggacc agcaaagctc ctctggaaag
     1741 gtgaaggggc agtagtaata caagataata gtgacataaa agtagtgcca agaagaaaag
     1801 caaagatcat tagggattat ggaaaacaga tggcaggtga tgattgtgtg gcaagtagac
     1861 aggatgagga ttagaacatg gaaaagttta gtaaaacacc ataaggagga gatatgaggg
     1921 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1981 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     2041 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     2101 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     2161 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     2221 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     2281 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     2341 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2401 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2461 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2521 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2581 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2641 ttcagaccca cctcccaacc ccgaggggac cgagctcaag cttcgaacgc gtgctagtta
     2701 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     2761 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     2821 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     2881 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     2941 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     3001 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     3061 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     3121 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     3181 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     3241 ggaggtctat ataagcagag ctcgtttagt gaaccgggct cgtttagtga accgaattct
     3301 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga
     3361 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3421 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3481 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3541 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3601 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3661 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3721 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3781 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3841 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3901 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3961 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4021 cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggaagcg gaggctccgg
     4081 gggaagtgcg gccgcttccc ggatggccgc ggaccttaac ttagagtgga ttagtttacc
     4141 acgcagttgg acctatggga tcacacgcgg agggcgcgtt tttttcatca atgaagaagc
     4201 taagtcaaca acctggcttc atccagtcac cggtgaggcg gtcgttactg gtcatcgtcg
     4261 ccagtcaaca gacctgccaa ctgggtggga agaagcgtat acgttcgagg gggcgcgtta
     4321 ttatattacc cttgaaacaa acccatacgt taagttactt ttggacgcca tgaaacactc
     4381 tggttgtgcg gtcaataaag accgtcactt ctcctgcgag gactgcaatg gcaatgtctc
     4441 gggtgggttt gatgcctcca cctcccaaat cgtgttgtgc cagaataata ttcacaacca
     4501 agcccacatg aaccgcgttg taacgcacga gctgatccac gcatttgacc actgtcgcgc
     4561 tcatgtcgat tggttcacca acattcgtca tttggcatgt tctgaggtac gtgctgcaaa
     4621 cctgtccggt gactgctcac tggtcaatga aatttttcgt ttacacttcg gtttgaaaca
     4681 gcatcatcag acgtgcgtcc gtgaccgtgc cacattgtcg atcttggcgg ttcgtaatat
     4741 tagcaaggag gtagctaaaa aggcggtgga tgaggtgttt gaatcctgtt ttaatgacca
     4801 cgaacctttc ggtcgcatcc cgcacaataa aacgtacgct cgctatgcac accgtgactt
     4861 cgagaatcgt gaccgttact attctaatat ttgaaccggt tctagataag attacggtac
     4921 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4981 ggggactgga agggctaatt cactcccaaa gaagacaaga tctgcttttt gcctgtactg
     5041 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5101 tgcttaagcc tcaataaagc ttcagctgct cgagctagca gatctttttc cctctgccaa
     5161 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     5221 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     5281 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     5341 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     5401 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     5461 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     5521 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     5581 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     5641 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     5701 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5761 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat
     5821 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5881 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5941 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
     6001 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6061 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6121 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6181 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6241 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6301 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     6361 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     6421 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6481 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6541 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6601 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6661 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6721 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6781 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     6841 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6901 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6961 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7021 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7081 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7141 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7201 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7261 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7321 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7381 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7441 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7501 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7561 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7621 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7681 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7741 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7801 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7861 aacccactc
//
