LOCUS       sequence-408157-        9363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PPP1CB_ALK from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PPP1CB_ALK
FEATURES             Location/Qualifiers
     source          1..9363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4124..4834,4835..4837,4838..4840))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4166..4187)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4515..4534
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4774..4795
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4837..4846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4922..5125)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4951..4971)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5126..5429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5686..5730)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5915..6148
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6508..6625
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7117..7242
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7289..7546
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7548..7927
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7958..8062
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8063..8131,8132..8923)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8281..8300)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9094..9363,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcacg
     1801 ggcctggctg attcatcgag tttttggact taaggatagt gtcttcataa tgtcccgcac
     1861 cgggcgccgt tgcagcctcc aggggcaagc cttgttgttg gtacccataa ttcacgtttc
     1921 cacatgggaa gtgacgcaga cgaaaaaggg gcacctcctt catattagct gtaagagatg
     1981 atggttccag aagcaaagat gcaccgggaa gacgaccggt tgccacattc ggcgggacag
     2041 tgcaagaccc ctcaaggccc aaattaccac ggtcgtgagg ttctttcttt gcgatgggat
     2101 tatttttctt agtaggtttc tcggtgaacc aacttccgta ggttggattc cacaaagacg
     2161 taggtttatt acgtgaacca tgtaccttgt gaagctcgga agggggatta gactgcgaga
     2221 aggccatgtt tacgtgaccc ccctccaccg caggaccacg tggtacgcgc acagaaatct
     2281 ctgccgcggt gggttttttc gcggccttcc cgctgctggt cgtcggcagg gggggtggtg
     2341 cagcggggga gcgctcctct tcacgctttg cttgttgact aacaagtaac ggggggacac
     2401 cctcggggtc tttcgggcga actgggactt tttcctcctc ctccactaat gggccgtact
     2461 cgatcggtaa ggctgtgtta atgacgtctg ggtcctgagt gcaatattca atacgttcca
     2521 agatgatcgc gaagtttggg cgatcctcgg gctggtgttg ccaacactgt gtcatgatgc
     2581 ggtacacagg cccagggcaa ttcttggggg ggtccatacg cccgcccgac gtcacaaact
     2641 ccaacacctc ctggttggat tttgagggat acggcatata ccctaaggaa aagatttccc
     2701 acagcaacac tccaaaactc caggtgtccg ttttggacgt aaaaatgcct tccataaagg
     2761 cctcgggagg catccattta accggcagca ttgcacagcc ccccttacga tagtaagaag
     2821 cgcggtaaat atcgcgtgcc atcccaaaat ctccgatctt cgccacacga cctggaccgg
     2881 ggcatgtaag aagacaatta cgggcagcaa tgtcacgatg gatgaagtga ttctcctcca
     2941 gatattgaca cccacaggcg atgtcgcggg ccacatgcaa aaggtcaagc attgccagag
     3001 aagacggttg gctaggacga gggcgagttt cgcgcaagaa ggacttcagg tcacctccag
     3061 ccatcagttc taataagata aagcggggca gggattgtaa cgatacgccg atacagcgaa
     3121 caatattttg gtgattaaac ttagaaatga tcagggcttc cataaggaaa tccaactcgt
     3181 cttgctcgct gcatacctcc ggcaaagttt tgactgcgac ttgtaaaggg gatgggtcat
     3241 ttggcatgcc gctgacttgg ccctcgtaga cttctccaaa agcgccgtga cccaagccac
     3301 gaatcaatgt aatattcttg cgaggcacct cttttaaatc ggagatggag ctagttttgc
     3361 cggcgaagca gtaattaggg ttataatccg tcataatggt gcttgtgcgc agtttactca
     3421 atttgtattc gggcgactgc agctccatct gcattgcttg taactcctgg tgcttacggc
     3481 gataaactgt gtcagggaca tccgtcggac gcataatgcg acgaatctgc tccattgact
     3541 gaagatctgg ggataagcca ccgtgacaac aaaagatctt ttcatcgacg atagcagcga
     3601 ttggcaagca attaaagcaa tcagtaaacg ttttccataa tttgatgtta aagcggcgtt
     3661 tgcactcatc ataaaacccg tagatgcggt tgatagaagc acactcgtgg tttccgcgca
     3721 ataaaaagaa attctctggg tatttgatct tgtaggccaa aagtaaacaa atcgtttcta
     3781 acgattgctt tccgcggtct acatagtcac ccaagaaaag gtagtttgct tccggtggga
     3841 aacctccata ctcgaacaga cgaagaaggt ctgtgtattg gccgtggatg tccccacaaa
     3901 ttttcagggg agcctccaac tctaaaagaa tcggctggct aagaaagatt tcgcgtgatt
     3961 taatgcacag tccgcgcacc tcggcttcgg tcatctgaac gattttccct gggcggcagc
     4021 cgcgcacctc aagtaagcgg gtaatcaagg agtcaacgtt cagttctccg tctgccattc
     4081 cccgggaagc ggccgcactt cccccggagc ctccgcttcc gcccttgtac agctcgtcca
     4141 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     4201 cgttggggtc tttgctcagc ttggactggg tgctcaggta gtggttgtcg ggcagcagca
     4261 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     4321 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
     4381 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     4441 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     4501 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     4561 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     4621 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
     4681 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     4741 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     4801 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc ggtggatccc
     4861 gggcccgcgg taccgtcgac tgcagaattc ggttcactaa acgagcccgg ttcactaaac
     4921 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     4981 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     5041 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     5101 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     5161 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     5221 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     5281 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     5341 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     5401 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     5461 accccgtaat tgattactat taataactag cacgcgttcg aagcttgagc tcggtcccct
     5521 cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac
     5581 tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa
     5641 taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt
     5701 atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa
     5761 ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg
     5821 attccaaatc tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt
     5881 gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct
     5941 ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag
     6001 atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa
     6061 taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc
     6121 tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac
     6181 tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt
     6241 atataattca cttctccaat tgtccctcat atctcctcct tatggtgttt tactaaactt
     6301 ttccatgttc taatcctcat cctgtctact tgccacacaa tcatcacctg ccatctgttt
     6361 tccataatcc ctaatgatct ttgcttttct tcttggcact acttttatgt cactattatc
     6421 ttgtattact actgcccctt cacctttcca gaggagcttt gctggtcctt tccaaagtgg
     6481 atttctgctg tccctgtaat aaacccgaaa attttgaatt tttgtaattt gtttttgtaa
     6541 ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg cactgtaccc
     6601 cccaatcccc ccttttcttt taaaattgtg gatgaatact gccatttgta ctgctgtctt
     6661 aagatgttca gcctgatctc ttacctgtcc tataattttc tttaattctt tattcataga
     6721 ttctactact ccttgacttt ggggattgta gggaatttcc aggtctgaag atcctgctgc
     6781 ttgctgtgct tttttcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt
     6841 ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa
     6901 tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct
     6961 acagccttct gatgtttcta acaggccagt attaactgcg aatcgttcta gctccctgct
     7021 tgcccatact atatgtttta atttatattt tttctttccc cctggcctta accgaatttt
     7081 ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc
     7141 cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct
     7201 tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt
     7261 cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt
     7321 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
     7381 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
     7441 gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg cttatatgca
     7501 ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctcccat ggtaatagcg
     7561 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
     7621 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
     7681 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
     7741 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
     7801 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
     7861 atatatgggc tatgaactaa tgaccccgta attgattact attaataact agtcaataat
     7921 caatgtcgac ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7981 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     8041 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     8101 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     8161 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     8221 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     8281 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     8341 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8401 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8461 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8521 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8581 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8641 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8701 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8761 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8821 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8881 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8941 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     9001 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     9061 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     9121 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     9181 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     9241 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     9301 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9361 cgg
//
