LOCUS       sequence-408183-        9660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, ZKSCAN5_SMO from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9660)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_ZKSCAN5_SMO
FEATURES             Location/Qualifiers
     source          1..9660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4421..5131,5132..5134,5135..5137))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4463..4484)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4812..4831
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5071..5092
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5134..5143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5219..5422)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5248..5268)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5423..5726
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5983..6027)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6212..6445
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6805..6922
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7414..7539
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7586..7843
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7845..8224
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8255..8359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8360..8428,8429..9220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8578..8597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9391..9660,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagat cagaagtcgg
     1801 agtctgcgtc cattaattca gtgtccatca agttggtgcg ggagtgaatt gggcccagtc
     1861 cttggcgacg tccgtgagcc caggcgacgg gagcgggtgc ggacggcaga aaagggtctt
     1921 gagggggact aggttcaggg cagaaagggt tagagaccaa ggtccacgct ccctgacggc
     1981 aagaatctcc cgcgccccat gcaccggccg cgactaaaca cttttgacga ggtaattggg
     2041 gaagacgagg aattgtggac ggcgcaggtg ccggggggtg taattccggt ggcggagcca
     2101 aagggcaaac ctccttctta cgcttgcggc gcttcttctt acgtcccagg cgtttctgaa
     2161 gctcgggtga gatctccgct tcaactaacc aaaggttcgc ttgctcctcg ggtggcaccg
     2221 gtgtagctac tggagtgacg gagatgtctt gcggtaaaat agcaccacga cgggccacca
     2281 tcttcgtgac atgttgggcc caagctgatg agacatcggc tgatggttca ttaagatcga
     2341 aagcaagtcc agctactggt ccgtcatggg ataccgtgtg catactaaag ctcaactctt
     2401 gtccgggatt ttgtaacagt tcgtggcgct tagagaaagc cttggcaatc atcttacttt
     2461 ttttaatacg cttgggctca tcatccgatt gccccgtcaa gcgacaccag gtacgacgcc
     2521 aaataagcaa ggtagccttc gtccacaccc acgtgctcat cgcaataccg gttccaaaca
     2581 ttgcaaacaa attgatcttt tcaaccagta agctcggacg attcttaatc tcgcagtccg
     2641 ggatgggttg ctttgtgggc agcccaattg tgacgtttgc ctggcacaac acataatcgc
     2701 ggaacgagcg ctcccattca gcctggttga aaaagtcgta gaaatgacag gagaatgtaa
     2761 tcagaacgaa gccaaatgcc aggaacccga agatcccaag gcgcaacatt gtttcattga
     2821 ttttgctggc agctttctcg gaaagcagcc caggatgatt cgacttgatt gagaacagcg
     2881 tcatcacacc gcgaattaag aagtaaccac ctacaatcag tactaaccca atgggagcca
     2941 gcacaaaccc cgcgcgatag cggtagtttt tgtatcctac gaaacagatc cctgatacgg
     3001 agtcgccatc tacttgtgca acggcaagaa ttgccaccgt aagcacgaat ggcagggacc
     3061 acgtcaacag atgaaagtag gaagttttac cggaaagcgg ctgatacgtt gtacccagcg
     3121 ccttgaacga cgtgtgccag gcgtacgtca gtactacaaa ccacaccaca ccagccatca
     3181 gtgcataata cacaataaca aagatgataa cacatgacag ggtttcatta ctagtaggct
     3241 cccccaaacg catcgtacca tcggcacgac atacgatctc gcggcgggca ccgtccatga
     3301 attgcgccag ccaaccaatc gaaccaacaa agaagcatgc gttgacgtag aataagataa
     3361 cggccgggta acgattcgag ttgcgccagt ctgcaacgaa tgtagctaac gtaaaaaggg
     3421 tacataaacc agtgacggcc ccaaatgctg caatatagct gtgcatgtct tggtgctccg
     3481 cttcggtgaa caatgggttt tggcactgaa taccacatcc ctccacatcc tcataccagc
     3541 tttttgggtt atccgtgcgg accaaaggaa cctcacactg accggagctg ttaaatttaa
     3601 tattctgtac ttcattagtg cacccttccg gaaagcgatc gggagtgcag cgcaggaagt
     3661 caggccatcc gcgttcacgt tctacgattg cgcacggccc acgtgtcgct tgacacaggg
     3721 tgcggctggg cagctccaca cgatcgttct cacacttagg catgtagacg gcgcaaagaa
     3781 gtggttgaat aacggcccag caacgcggag cattacgcag gccattttcc tccagcaagc
     3841 gtggtttctg gggagcttgc tccggttggg tgtcaaccgt cagagggtga ggagagcacg
     3901 attcctgaac tgctcctggc gtggccattt tacgcggaag cacgtccggg caggccacaa
     3961 tctgttggcg acgctcttct aattcgcgtt ggatgttttc aataactgcc actgcttctt
     4021 ccccgctctc tgggtgatgc tcacggaccc aaggttgaaa ttcctcagga aggatcgtca
     4081 agaactgttc aagcactaac aactcaagga tttgctcttt cgtgtgaagc tcggggcgca
     4141 gccactcaca acacaagaca cgaagctggc ttaacgcttc gcgaggtccg gatgcctcat
     4201 ggtattggaa gtgacgaaaa cgctgataga aagtttcgaa tgtcggggga ttatattctt
     4261 gcatccacgt gcaatcctcc tcctcgacct tgacgataaa aaggtcttct tgctcctggg
     4321 acgtctccgc tggaggatct aaatcaatta cttcacgcga ctctgtcatg atcattcccc
     4381 gggaagcggc cgcacttccc ccggagcctc cgcttccgcc cttgtacagc tcgtccatgc
     4441 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
     4501 tggggtcttt gctcagcttg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
     4561 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
     4621 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
     4681 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
     4741 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
     4801 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
     4861 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
     4921 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
     4981 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
     5041 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
     5101 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg
     5161 cccgcggtac cgtcgactgc agaattcggt tcactaaacg agcccggttc actaaacgag
     5221 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     5281 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     5341 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     5401 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     5461 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     5521 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     5581 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     5641 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     5701 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     5761 ccgtaattga ttactattaa taactagcac gcgttcgaag cttgagctcg gtcccctcgg
     5821 ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat
     5881 agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa
     5941 ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc
     6001 taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga
     6061 gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt
     6121 ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag cagtggtgca
     6181 aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc
     6241 cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg
     6301 ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa
     6361 ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc
     6421 caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct
     6481 tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata
     6541 taattcactt ctccaattgt ccctcatatc tcctccttat ggtgttttac taaacttttc
     6601 catgttctaa tcctcatcct gtctacttgc cacacaatca tcacctgcca tctgttttcc
     6661 ataatcccta atgatctttg cttttcttct tggcactact tttatgtcac tattatcttg
     6721 tattactact gccccttcac ctttccagag gagctttgct ggtcctttcc aaagtggatt
     6781 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc
     6841 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc
     6901 aatcccccct tttcttttaa aattgtggat gaatactgcc atttgtactg ctgtcttaag
     6961 atgttcagcc tgatctctta cctgtcctat aattttcttt aattctttat tcatagattc
     7021 tactactcct tgactttggg gattgtaggg aatttccagg tctgaagatc ctgctgcttg
     7081 ctgtgctttt ttcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt
     7141 gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga
     7201 tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca
     7261 gccttctgat gtttctaaca ggccagtatt aactgcgaat cgttctagct ccctgcttgc
     7321 ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc
     7381 ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt
     7441 ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc
     7501 cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc
     7561 tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg
     7621 agggatctct agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa
     7681 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
     7741 cagatctggt ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga
     7801 tctgagggct cgccactccc cagtcccgcc caggccacgc ctcccatggt aatagcgatg
     7861 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     7921 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     7981 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     8041 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     8101 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
     8161 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
     8221 tgtcgaccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8281 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8341 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8401 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8461 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8521 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8581 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8641 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8701 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8761 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8821 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8881 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8941 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     9001 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     9061 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     9121 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     9181 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9241 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9301 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9361 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9421 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9481 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9541 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9601 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
//
