LOCUS       sequence-408250-       15151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BglBrick plasmid 
            (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV 
            pathway enzymes to produce limonene from glucose in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15151)
  AUTHORS   Alonso-Gutierrez J, Chan R, Batth TS, Adams PD, Keasling JD, Petzold
            CJ, Lee TS
  TITLE     Metabolic engineering of Escherichia coli for limonene and perillyl 
            alcohol production.
  JOURNAL   Metab Eng. 2013 May 29;19C:33-41. doi: 10.1016/j.ymben.2013.05.004.
  PUBMED    23727191
REFERENCE   2  (bases 1 to 15151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2013-05-29"; volume: "19C:33-41. doi"; pages: " 
            10.1016/j.ymben.2013.05.004"
COMMENT     Sequence Label: pJBEI-6410
FEATURES             Location/Qualifiers
     source          1..15151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(49..1131)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1093..1112
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1132..1209)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1415..1434
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(1520..1525,1526..1543,1544..1550)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1558..1574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(2309..2320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      5322..5393
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5409..5436
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        join(5649..5654,5655..5671,5672..5678)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    5686..5702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8725..8736
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(10434..10439,10440..10456,10457..10463)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    10471..10487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      13102..13173
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      13189..13216
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(13244..13261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13378..13923)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(13458..13477)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(14037..14131)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(14161..14952,14953..15021))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14784..14803
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15022..15126)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
      301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
      361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
      601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
      661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
      721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
      781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
      841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
      901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
      961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
     1561 tgagcggata acaatttcag aattcaaaag atcttaggag gaatataaaa tgaaaaattg
     1621 tgtcatcgtc agtgcggtac gtactgctat cggtagtttt aacggttcac tcgcttccac
     1681 cagcgccatc gacctggggg cgacagtaat taaagccgcc attgaacgtg caaaaatcga
     1741 ttcacaacac gttgatgaag tgattatggg taacgtgtta caagccgggc tggggcaaaa
     1801 tccggcgcgt caggcactgt taaaaagcgg gctggcagaa acggtgtgcg gattcacggt
     1861 caataaagta tgtggttcgg gtcttaaaag tgtggcgctt gccgcccagg ccattcaggc
     1921 aggtcaggcg cagagcattg tggcgggggg tatggaaaat atgagtttag ccccctactt
     1981 actcgatgca aaagcacgct ctggttatcg tcttggagac ggacaggttt atgacgtaat
     2041 cctgcgcgat ggcctgatgt gcgccaccca tggttatcat atggggatta ccgccgaaaa
     2101 cgtggctaaa gagtacggaa ttacccgtga aatgcaggat gaactggcgc tacattcaca
     2161 gcgtaaagcg gcagccgcaa ttgagtccgg tgcttttaca gccgaaatcg tcccggtaaa
     2221 tgttgtcact cgaaagaaaa ccttcgtctt cagtcaagac gagttcccga aagcgaactc
     2281 aacggctgaa gcgttaggtg cattgcgccc ggccttcgat aaagcaggaa cagtcaccgc
     2341 tgggaacgcg tctggtatta acgacggtgc tgccgctctg gtgattatgg aagaatctgc
     2401 ggcgctggca gcaggcctta cccccctggc tcgcattaaa agttatgcca gcggtggcgt
     2461 gccccccgca ttgatgggta tggggccagt acctgccacg caaaaagcgt tacaactggc
     2521 ggggctgcaa ctggcggata ttgatctcat tgaggctaat gaagcatttg ctgcacagtt
     2581 ccttgccgtt gggaaaaacc tgggctttga ttctgagaaa gtgaatgtca acggcggggc
     2641 catcgcgctc gggcatccta tcggtgccag tggtgctcgt attctggtca cactattaca
     2701 tgccatgcag gcacgcgata aaacgctggg gctggcaaca ctgtgcattg gcggcggtca
     2761 gggaattgcg atggtgattg aacggttgaa ttgaggatct attcaggaga aaccttatga
     2821 caataggtat cgataaaata aacttttacg ttccaaagta ctatgtagac atggctaaat
     2881 tagcagaagc acgccaagta gacccaaaca aatttttaat tggaattggt caaactgaaa
     2941 tggctgttag tcctgtaaac caagacatcg tttcaatggg cgctaacgct gctaaggaca
     3001 ttataacaga cgaagacaaa aagaaaattg gtatggtaat tgtggcaact gaatcagcag
     3061 ttgatgctgc taaagcagcc gctgttcaaa ttcacaactt attaggtatt caaccttttg
     3121 cacgctgctt tgaaatgaaa gaagcttgtt atgctgcaac accagcaatt caattagcta
     3181 aagattattt agcaactaga ccgaatgaaa aagtattagt tattgctaca gatacagcac
     3241 gttatggatt gaactcaggc ggcgagccaa cacaaggtgc tggcgcagtt gcgatggtta
     3301 ttgcacataa tccaagcatt ttggcattaa atgaagatgc tgttgcttac actgaagacg
     3361 tttatgattt ctggcgtcca actggacata aatatccatt agttgatggt gcattatcta
     3421 aagatgctta tatccgctca ttccaacaaa gctggaatga atacgcaaaa cgtcaaggta
     3481 agtcgctagc tgacttcgca tctctatgct tccatgttcc atttacaaaa atgggtaaaa
     3541 aggcattaga gtcaatcatt gataacgctg atgaaacaac tcaagagcgt ttacgttcag
     3601 gatatgaaga tgctgtagat tataaccgtt atgtcggtaa tatttatact ggatcattat
     3661 atttaagcct aatatcatta cttgaaaatc gagatttaca agctggtgaa acaatcggtt
     3721 tattcagtta tggctcaggt tcagttggtg aattttatag tgcgacatta gttgaaggct
     3781 acaaagatca tttagatcaa gctgcacata aagcattatt aaataaccgt actgaagtat
     3841 ctgttgatgc atatgaaaca ttcttcaaac gttttgatga cgttgaattt gacgaagaac
     3901 aagatgctgt tcatgaagat cgtcatattt tctacttatc aaatattgaa aataacgttc
     3961 gcgaatatca cagaccagag taattaggat ctattcagga aacagaccat gtccatgcaa
     4021 agtttagata agaattttcg acatttatct cgtaaagaaa agttacaaca attggttgat
     4081 aagcaatggt tatcagaaga acaattcgac attttactga atcatccatt aatcgatgaa
     4141 gaagtagcca atagtttaat tgaaaatgtc atcgcgcaag gtgcattacc cgttggatta
     4201 ttaccgaata tcattgtgga cgataaggca tatgttgtac ctatgatggt ggaagagcct
     4261 tcagttgtcg ctgcagctag ttatggtgca aagctagtga atcagactgg cggatttaaa
     4321 acggtatctt ctgaacgtat tatgataggt caaatcgtct ttgatggcgt tgacgatact
     4381 gaaaaattat cagcagacat taaagcttta gaaaagcaaa ttcataaaat tgcggatgag
     4441 gcatatcctt ctattaaagc gcgtggtggt ggttaccaac gtatagcgat tgatacattt
     4501 cctgagcaac agttactatc tttaaaagta tttgttgata cgaaagatgc tatgggcgct
     4561 aatatgctta atacgatttt agaggccata actgcatttt taaaaaatga atttccgcaa
     4621 agcgacattt taatgagtat tttatccaat catgcaacag cgtccgttgt taaagttcaa
     4681 ggcgaaattg atgttaaaga tttagcaagg ggcgagagaa ctggagaaga ggttgccaaa
     4741 cgaatggaac gtgcttctgt attggcccaa gtagatattc atcgtgcagc aacacataat
     4801 aaaggtgtta tgaatggcat acatgctgtt gttttagcaa caggaaatga tacgcgtggt
     4861 gcagaagcaa gtgcgcatgc atacgcgagt cgtgacggac agtatcgtgg tattgctaca
     4921 tggcgttacg atcaagatcg tcaacgattg attggtacaa ttgaagtgcc tatgacattg
     4981 gcaatcgttg gcggtggtac aaaagtatta ccaattgcta aagcttcatt agagctacta
     5041 aatgtagagt cagcacaaga attaggtcat gtagttgctg ccgttggttt agcgcaaaac
     5101 tttgcagcat gtcgcgcgct tgtgtcagaa ggtattcaac aaggtcatat gagtttacaa
     5161 tataaatcat tagctatcgt tgtaggggca aaaggtgatg aaattgctaa agtagctgaa
     5221 gctttgaaaa aagaaccccg tgcaaataca caagcagcgg aacatatttt acaagaaatt
     5281 agacaacaat aattaggatc tttttaagga tctccaggca tcaaataaaa cgaaaggctc
     5341 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     5401 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tagcgaattg gatctggttt
     5461 gacagcttat catcgactgc acggtgcacc aatgcttctg gcgtcaggca gccatcggaa
     5521 gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac
     5581 tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa atattctgaa
     5641 atgagctgtt gacaattaat catccggctc gtataatgtg tggaattgtg agcggataac
     5701 aatttcagga tctaggagga aataaccatg tctctgccat tcctgacgtc tgcgccaggt
     5761 aaggtgatca tcttcggcga gcactctgcg gtgtacaata agccggccgt cgccgcctct
     5821 gtgtctgcgt tacgcaccta cctgctgatc agcgaatctt ctgcaccgga cacgatcgag
     5881 ctggactttc cggacatcag cttcaaccac aagtggagca tcaacgactt caacgcgatc
     5941 acggaggacc aggtgaacag ccaaaagctg gccaaagccc agcaagcaac cgacggtctg
     6001 tctcaggagc tggtgtctct gctggacccg ctgttagcgc agttaagcga gagcttccat
     6061 taccacgccg cgttctgctt cctgtacatg ttcgtttgcc tgtgcccgca cgcaaagaac
     6121 atcaagttca gcctgaagag cacgctgccg attggcgcag gcttaggctc tagcgcatct
     6181 atcagcgtga gcctggcgct ggcgatggcc tatctgggtg gcctgattgg cagcaacgac
     6241 ctggagaaac tgagcgaaaa cgacaagcac atcgtgaacc agtgggcctt tatcggcgag
     6301 aagtgcattc atggcacccc gagcggcatt gacaacgcag ttgccacgta tggcaacgcc
     6361 ctgctgttcg agaaagacag ccacaacggc acgatcaaca cgaacaactt caagttcctg
     6421 gacgacttcc cggcgatccc gatgattctg acctacaccc gtatcccacg cagcaccaag
     6481 gatttagtcg cccgcgtgcg tgttttagtc accgaaaagt tcccggaggt gatgaagccg
     6541 atcctggacg cgatgggcga gtgcgcgctg cagggtctgg agatcatgac caagctgagc
     6601 aagtgcaagg gcaccgacga tgaggcggtg gagaccaaca atgagctgta cgagcagctg
     6661 ctggagctga tccgtatcaa tcacggcctg ctggtctcta tcggtgtgtc tcacccgggc
     6721 ctggaactga tcaaaaacct gagcgacgac ctgcgcattg gctctacgaa attaacgggt
     6781 gcaggtggcg gtggctgctc tttaacgctg ctgcgcagtg acattacgca ggagcaaatc
     6841 gacagcttca agaagaagct gcaggacgac ttcagctacg agacgttcga gacggacctg
     6901 ggcggcacgg gctgttgcct gctgagcgcc aaaaatctga acaaggacct gaagatcaaa
     6961 agcctggtgt tccagctgtt cgaaaacaag acgaccacga agcagcagat cgacgacctg
     7021 ttactgccgg gtaacaccaa tctgccgtgg acgtcttgag gatctaggag ggagatcata
     7081 tgagcgaatt acgtgcattc agcgcgccag gtaaggcact gctggccggt ggctacctgg
     7141 tgttagacac caagtacgag gcgttcgtcg tcggcttatc tgcccgtatg catgcagttg
     7201 cccacccgta tggtagcctg cagggctctg acaagttcga agtgcgtgtg aagagcaagc
     7261 agttcaagga cggcgagtgg ctgtaccaca ttagcccaaa gagcggcttc atcccggtta
     7321 gcattggtgg cagcaagaac ccatttatcg agaaggtcat tgccaacgtc ttcagctact
     7381 tcaagccgaa tatggacgat tactgcaacc gcaacctgtt cgtcatcgac attttcagcg
     7441 acgacgcgta ccacagccaa gaggactctg ttacggagca tcgtggtaac cgccgcctga
     7501 gcttccacag ccatcgcatt gaggaggtgc cgaagacggg tctgggttct agcgccggtt
     7561 tagttaccgt cttaacgacg gcgttagcga gcttcttcgt gagcgacctg gagaacaacg
     7621 tggacaagta ccgcgaagtg attcataacc tggcgcaggt ggcacattgt caggcccaag
     7681 gtaagattgg ctctggtttt gatgtggcag cggccgccta tggctctatc cgctatcgcc
     7741 gctttccgcc ggccctgatc agcaatctgc cggacatcgg ctctgcgacg tatggtagca
     7801 aactggcgca tctggtggac gaggaggact ggaacatcac cattaagtct aatcacctgc
     7861 cgagcggctt aacgttatgg atgggcgata tcaagaacgg cagcgaaacg gttaagctgg
     7921 tgcagaaagt gaaaaactgg tacgacagcc acatgccgga aagcctgaag atttacacgg
     7981 agctggacca cgccaatagc cgtttcatgg atggtctgag caagctggac cgcctgcacg
     8041 aaacccacga cgactacagc gaccaaatct tcgagagcct ggagcgcaat gactgcacct
     8101 gccagaagta cccggagatc acggaggtcc gcgatgccgt ggcaacgatt cgccgtagct
     8161 tccgcaaaat tacgaaggag agcggcgcgg atatcgaacc accggtccag acgtctctgc
     8221 tggacgactg tcaaacctta aagggcgtgt taacgtgcct gattccgggc gcgggtggtt
     8281 acgacgccat tgccgtcatc acgaaacagg acgtcgatct gcgcgcacaa acggccaacg
     8341 acaaacgttt cagcaaagtc caatggctgg atgttacgca ggccgactgg ggtgttcgca
     8401 aggagaagga cccggaaacg tatctggata agtgaggatc taggaggatt atgagatgac
     8461 cgtttacaca gcatccgtta ccgcacccgt caacatcgca acccttaagt attgggggaa
     8521 aagggacacg aagttgaatc tgcccaccaa ttcgtccata tcagtgactt tatcgcaaga
     8581 tgacctcaga acgttgacct ctgcggctac tgcacctgag tttgaacgcg acactttgtg
     8641 gttaaatgga gaaccacaca gcatcgacaa tgaaagaact caaaattgtc tgcgcgacct
     8701 acgccaatta agaaaggaaa tggaatcgaa ggacgcctca ttgcccacat tatctcaatg
     8761 gaaactccac attgtctccg aaaataactt tcctacagca gctggtttag cttcctccgc
     8821 tgctggcttt gctgcattgg tctctgcaat tgctaagtta taccaattac cacagtcaac
     8881 ttcagaaata tctagaatag caagaaaggg gtctggttca gcttgtagat cgttgtttgg
     8941 cggatacgtg gcctgggaaa tgggaaaagc tgaagatggt catgattcca tggcagtaca
     9001 aatcgcagac agctctgact ggcctcagat gaaagcttgt gtcctagttg tcagcgatat
     9061 taaaaaggat gtgagttcca ctcagggtat gcaattgacc gtggcaacct ccgaactatt
     9121 taaagaaaga attgaacatg tcgtaccaaa gagatttgaa gtcatgcgta aagccattgt
     9181 tgaaaaagat ttcgccacct ttgcaaagga aacaatgatg gattccaact ctttccatgc
     9241 cacatgtttg gactctttcc ctccaatatt ctacatgaat gacacttcca agcgtatcat
     9301 cagttggtgc cacaccatta atcagtttta cggagaaaca atcgttgcat acacgtttga
     9361 tgcaggtcca aatgctgtgt tgtactactt agctgaaaat gagtcgaaac tctttgcatt
     9421 tatctataaa ttgtttggct ctgttcctgg atgggacaag aaatttacta ctgagcagct
     9481 tgaggctttc aaccatcaat ttgaatcatc taactttact gcacgtgaat tggatcttga
     9541 gttgcaaaag gatgttgcca gagtgatttt aactcaagtc ggttcaggcc cacaagaaac
     9601 aaacgaatct ttgattgacg caaagactgg tctaccaaag gaataaggat ctaggaggta
     9661 atgataatgc aaacggaaca cgtcatttta ttgaatgcac agggagttcc cacgggtacg
     9721 ctggaaaagt atgccgcaca cacggcagac acccgcttac atctcgcgtt ctccagttgg
     9781 ctgtttaatg ccaaaggaca attattagtt acccgccgcg cactgagcaa aaaagcatgg
     9841 cctggcgtgt ggactaactc ggtttgtggg cacccacaac tgggagaaag caacgaagac
     9901 gcagtgatcc gccgttgccg ttatgagctt ggcgtggaaa ttacgcctcc tgaatctatc
     9961 tatcctgact ttcgctaccg cgccaccgat ccgagtggca ttgtggaaaa tgaagtgtgt
    10021 ccggtatttg ccgcacgcac cactagtgcg ttacagatca atgatgatga agtgatggat
    10081 tatcaatggt gtgatttagc agatgtatta cacggtattg atgccacgcc gtgggcgttc
    10141 agtccgtgga tggtgatgca ggcgacaaat cgcgaagcca gaaaacgatt atctgcattt
    10201 acccagctta aataattagg atctcgcaaa aaaccccgct tcggcggggt tttttcgccg
    10261 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg
    10321 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa
    10381 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa
    10441 ttaatcatcc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt caggatcttt
    10501 taaagaagga gatatacata tggaatttga cttcaacaaa tacatggact ccaaagcgat
    10561 gacggtaaat gaagcactga acaaagcgat ccctctgcgt tatccgcaga aaatctacga
    10621 aagcatgcgt tacagcctgc tggcaggcgg caagcgtgtt cgtccggttc tgtgtattgc
    10681 cgcatgtgaa ctggtaggtg gtaccgaaga actggcgatc ccgaccgcgt gcgcaattga
    10741 aatgatccac acgatgtccc tgatgcacga tgatctgccg tgtatcgaca acgacgatct
    10801 gcgtcgcggt aaaccgacta accacaaaat tttcggtgag gataccgcag tgactgctgg
    10861 taacgcactg cactcttacg ccttcgagca tatcgcggtt tctacttcta aaaccgttgg
    10921 tgctgaccgc atcctgcgta tggtgtccga gctgggtcgt gctactggct ctgaaggtgt
    10981 tatgggtggt cagatggtag acatcgcatc cgaaggcgat ccgtctatcg acctgcagac
    11041 cctggaatgg attcacatcc acaaaaccgc aatgctgctg gaatgctccg ttgtttgcgg
    11101 tgcaatcatt ggcggtgcca gcgaaatcgt aatcgaacgt gcccgtcgct acgcccgctg
    11161 tgttggtctg ctgttccagg tagttgatga cattctggac gtaactaaaa gcagcgacga
    11221 actgggtaag actgcgggca aggacctgat ctctgataaa gccacctacc caaagctgat
    11281 gggtctggaa aaggccaagg agttctccga tgaactgctg aaccgtgcga agggtgaact
    11341 gtcctgcttc gacccagtta aagccgctcc gctgctgggc ctggcagact acgtggcatt
    11401 tcgtcagaat taaggtactt ttaagaagga gatatacatg cgtcgcagtg gtaattacaa
    11461 cccgtcacgt tgggatgtga attttatcca atccctgctg agcgattaca aagaggacaa
    11521 acacgtcatt cgggcaagcg aactggttac actggttaaa atggaactgg aaaaagaaac
    11581 agaccaaatt cgccaattag agctgattga cgacttacag cgcatggggc tgagcgatca
    11641 ctttcagaat gagttcaaag agattctgtc atctatctat ctggaccacc attactataa
    11701 aaaccctttt ccgaaagagg aacgtgatct gtatagcaca agcctggcct ttcggttact
    11761 gcgtgagcat ggcttccaag tagcacaaga ggtattcgat agcttcaaga atgaagaggg
    11821 cgagttcaaa gaatcactgt ccgatgatac tcgtggctta ttacaactgt atgaagcgag
    11881 ctttctgctg accgaggggg agacgactct ggaaagcgca cgggaatttg cgaccaaatt
    11941 cttagaagag aaagtaaatg aaggcggcgt agatggcgat ctgttaaccc gcatcgcgta
    12001 ttctctggac attccgttac actggcgtat taaacggcca aacgcgccgg tgtggattga
    12061 gtggtatcgc aaacgtccag acatgaaccc ggtggtgctg gaattagcaa ttttagactt
    12121 aaacatcgtg caggctcaat ttcaggaaga actgaaagaa tcttttcgct ggtggcgcaa
    12181 caccggcttc gttgaaaaat taccgtttgc gcgcgatcgt ctggttgaat gttatttctg
    12241 gaataccggc attatcgaac cgcgccagca cgcgagcgcc cgtattatga tgggtaaggt
    12301 caatgcgctg attactgtca ttgacgatat ttacgatgta tacggcactc tggaagaact
    12361 ggagcagttt acagatttaa tccgccggtg ggacatcaat agcatcgacc agctgccaga
    12421 ttacatgcag ctgtgttttc tggcgctgaa taactttgtg gacgacacct cttatgacgt
    12481 catgaaagaa aaaggcgtga atgttatccc ttatctgcgt cagagttggg tggatctggc
    12541 cgataaatat atggttgagg cacgctggtt ctacggtggg cacaaaccga gtttagaaga
    12601 atatctggag aactcatggc agagtatcag tgggccatgc atgctgacgc atatcttctt
    12661 tcgcgtcacg gattcattta ccaaggagac ggtcgattcc ttatataaat accatgacct
    12721 ggttcgttgg tcatctttcg tcctgcggct ggcggacgat ctgggtacgt ctgtggaaga
    12781 ggtgtctcgt ggggatgttc cgaaatcctt acagtgctac atgtccgatt ataacgcaag
    12841 tgaagctgaa gctcgcaaac atgtgaagtg gctgatcgcc gaagtgtgga agaagatgaa
    12901 cgctgaacgt gtttccaagg atagtccgtt tggtaaggat tttattggtt gcgccgttga
    12961 tctgggtcgt atggcccagc tgatgtatca taacggtgat ggtcatggga cccagcatcc
    13021 tattattcat cagcagatga cgcgtaccct gtttgcacct ttcgcctaag gatccaaact
    13081 cgagtaagga tctccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
    13141 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
    13201 ggtgggcctt tctgcgttta tacctaggga tatattccgc ttcctcgctc actgactcgc
    13261 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
    13321 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt
    13381 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
    13441 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc
    13501 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
    13561 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
    13621 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    13681 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta
    13741 gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct
    13801 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg
    13861 ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct
    13921 caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct
    13981 tcaaatgtag cacctgaagt cagccccata cgatataagt tgttactagt gcttggattc
    14041 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
    14101 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgtaaactt ggtctgacag
    14161 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    14221 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    14281 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    14341 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    14401 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    14461 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    14521 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    14581 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    14641 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    14701 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    14761 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    14821 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    14881 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    14941 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    15001 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    15061 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    15121 tccgcgcaca tttccccgaa aagtgccacc t
//
