LOCUS       sequence-408251-        3008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of CsgA with C-terminal SpyTag peptide and GS 
            linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ozkul G, Kehribar ES, Ahan RE, Koksaldi IC, Ozkul A, Dinc B, Aydogan
            S, Seker UOS
  TITLE     A Genetically Engineered Biofilm Material for SARS-CoV-2 Capturing 
            and Isolation.
  JOURNAL   Adv Mater Interfaces. 2022 Sep 13:2201126. doi: 
            10.1002/admi.202201126.
  PUBMED    36248312
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Adv Mater 
            Interfaces. 2022 Sep 13:2201126. doi: 10.1002/admi.202201126."
COMMENT     Sequence Label: pZa pLtetO CsgA-SpyTag
FEATURES             Location/Qualifiers
     source          1..3008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    1007..1025
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1032..1050
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(1303..1321,1322..1327,1328..1344,1345..1350,
                     1351..1376)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1303..1321
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1328..1346
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             1411..1422
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1894..1911
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1918..1956
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     terminator      1971..2057
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2092..2109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2226..2771)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2306..2325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2885..2979)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtcaaa gggccctccc tatcagtgat
     1021 agagattgac atccctatca gtgatagaga tactgagcac agtcgacacc caaatccagg
     1081 aggtgattgg tagtggtggt taatgaaaat taacttacta ctaccatata tctctagatg
     1141 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
     1201 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca
     1261 aataaaacga aaggctcagt cgaaagactg ggccttctcg agtccctatc agtgatagag
     1321 attgacatcc ctatcagtga tagagatact gagcacatca gcaggacgca ctgaccgaat
     1381 tctaccattc acctcttgga tttgggtatt aaagaggaga aaggtaccat gaaactttta
     1441 aaagtagcag caattgcagc aatcgtattc tccggtagcg ctctggcagg tgttgttcct
     1501 cagtacggcg gcggcggtaa ccacggtggt ggcggtaata atagcggccc aaattctgag
     1561 ctgaacattt accagtacgg tggcggtaac tctgcacttg ctctgcaaac tgatgcccgt
     1621 aactctgact tgactattac ccagcatggc ggcggtaatg gtgcagatgt tggtcagggc
     1681 tcagatgaca gctcaatcga tctgacccaa cgtggcttcg gtaacagcgc tactcttgat
     1741 cagtggaacg gcaaaaattc tgaaatgacg gttaaacagt tcggtggtgg caacggtgct
     1801 gcagttgacc agactgcatc taactcctcc gtcaacgtga ctcaggttgg ctttggtaac
     1861 aacgcgaccg ctcatcagta cctcgagggt agccaccacc accaccacca cggatccgcg
     1921 catatcgtga tggtggatgc gtataaaccg accaaatgaa cgcgtggcat caaataaaac
     1981 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2041 tcctgagtag gacaaatccg ccgccctaga cctagggata tattccgctt cctcgctcac
     2101 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg
     2161 agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag
     2221 ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag
     2281 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc
     2341 gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt
     2401 ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
     2461 tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga
     2521 gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta attgatttag
     2581 aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg
     2641 actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg
     2701 aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa
     2761 aacgatctca agaagatcat cttattaatc agataaaata tttctagatt tcagtgcaat
     2821 ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg ttactagtgc
     2881 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     2941 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gtaaacttgg
     3001 tctgacag
//
