LOCUS       sequence-408252-        3691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cadmium responsive genetic circuit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Akboga D, Saltepe B, Bozkurt EU, Seker UOS
  TITLE     A Recombinase-Based Genetic Circuit for Heavy Metal Monitoring.
  JOURNAL   Biosensors (Basel). 2022 Feb 16;12(2):122. doi: 
            10.3390/bios12020122.
  PUBMED    35200383
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosensors 
            (Basel)."; date: "2022-02-16"; volume: "12(2):122. doi"; pages: " 
            10.3390/bios12020122"
COMMENT     Sequence Label: PdnaK-IR3-IR3-Bxb1-CadR-pZa
FEATURES             Location/Qualifiers
     source          1..3691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             266..277
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             1421..1432
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1894..1980
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2015..2032)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2149..2694)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2229..2248)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2808..2902)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2923..3582)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3497..3516
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3583..3685)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 cattttcgcc agatatcgac gtcctcgaga aaagcacaaa aaatttttgc atctccccct
       61 tgatgacgtg gtttacgacc ccatttagta gtcaaccgca gtgagtgagt ctgcaaaaaa
      121 atgaaattgg gcagttgaaa ccagacgttt cgcccctatt acagactcac aaccacatga
      181 tgaccgaata tatagtggag acgtttagat ggcaagcttg agcggggtgc actcatcagc
      241 aagcttgagc ggggtgcact catcaaaaga ggagaaaggt accatgggaa cggtggcgca
      301 gatggaatta gaagcgatca aagagcggaa ccgttcggct gcgcatttca atatccgcgc
      361 cgggaaatac cgaggatccc tgccgccgtg gggatacctg cctacgcgcg tggacgggga
      421 gtggcggctg gtgccggacc ctgtgcagcg agagcgcatc ctcgaggtgt atcaccgcgt
      481 cgtcgacaac cacgagccgc tgcatctggt ggcccacgac ctgaaccggc gtggtgtcct
      541 gtcgccgaag gactacttcg cgcagctgca aggccgcgag ccgcagggcc gggagtggtc
      601 ggctaccgcg ctgaagcgat cgatgatctc cggggcgatg ctcgggtacg cgactctgaa
      661 cggtaagacc gtccgagacg acgacggagc cccgctggtg cgggctgagc cgatcctgac
      721 ccgtgagcag ctggaggcgc tgcgcgccga gctcgtgaag acctcccggg cgaagcccgc
      781 ggtgtctacc ccgtcgctgc tgctgcgggt gttgttctgc gcggtgtgcg gggagcccgc
      841 gtacaagttc gccgggggag gacgtaagca cccgcgctac cgctgccgct cgatggggtt
      901 cccgaagcac tgcgggaacg gcacggtggc gatggccgag tgggacgcgt tctgcgagga
      961 gcaggtactg gatctgctcg gggacgcgga gcgtctggag aaagtctggg tagcgggctc
     1021 ggactccgcg gtcgaactcg cggaggtgaa cgcggagctg gtggacctaa cgtcgctgat
     1081 cggctccccg gcctaccggg cgggctctcc gcagcgagaa gcactggatg cccgtattgc
     1141 ggcgctggcc gcgcggcaag aggagctgga gggcctggag gctcgcccgt ctggctggga
     1201 gtggcgcgag accgggcagc ggttcgggga ctggtggcgg gagcaggaca ccgcggcaaa
     1261 gaacacctgg cttcggtcga tgaacgttcg gctgacgttc gacgtccgcg gcgggctgac
     1321 tcgcacgatc gacttcgggg atcttcagga gtacgagcag catctcaggc tcggcagcgt
     1381 ggtcgaacgg ctacacaccg ggatgtcgta aacgcgtatt aaagaggaga aaggtaccat
     1441 gaagatcgga gaactggcca aagccaccga ctgcgccgtg gaaaccatcc gctactacga
     1501 gcgtgaacag ctgctgccgg agccggcacg cagcgacggc aactaccggc tgtacaccca
     1561 ggcccacgtc gagcggctta ccttcatccg caactgccgc accctggaca tgaccctgga
     1621 tgaaatccgc agcctgctac gcctgcgcga cagccccgat gattcgtgcg gcagcgtcaa
     1681 tgcgctgatc gacgagcata tcgagcatgt gcaggcacgg atcgatggtc tggtggcgtt
     1741 gcaggaacag ctggtggagc tgcggcggcg ctgcaatgca caaggggcgg agtgtgcgat
     1801 cttgcagcaa ctggagacga acggggcggt atcggtgccg gaaaccgagc attcgcatgt
     1861 agggcgaagc cacgggcatt aactgcaggg catcaaataa aacgaaaggc tcagtcgaaa
     1921 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     1981 ccgccgccct agacctaggg atatattccg cttcctcgct cactgactcg ctacgctcgg
     2041 tcgttcgact gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg
     2101 ccaggaagat acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct
     2161 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac
     2221 aggactataa agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc
     2281 tgcctttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc
     2341 tgacactcag ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
     2401 ttcagtccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac
     2461 atgcaaaagc accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag
     2521 tcatgcgccg gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc
     2581 cagttacctc ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag
     2641 gcggtttttt cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat
     2701 catcttatta atcagataaa atatttctag atttcagtgc aatttatctc ttcaaatgta
     2761 gcacctgaag tcagccccat acgatataag ttgttactag tgcttggatt ctcaccaata
     2821 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     2881 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
     2941 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     3001 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     3061 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     3121 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     3181 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     3241 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     3301 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     3361 acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     3421 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     3481 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     3541 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     3601 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     3661 gttggaacct cttacgtgcc gatcaacgtc t
//
