LOCUS       sequence-408286-        9018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express EPN24-MCP-I-T2A-eGFP and blasticidin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Horns F, Martinez JA, Fan C, Haque M, Linton JM, Tobin V, Santat L, 
            Maggiolo AO, Bjorkman PJ, Lois C, Elowitz MB
  TITLE     Engineering RNA export for measurement and manipulation of living 
            cells.
  JOURNAL   Cell. 2023 Jul 10:S0092-8674(23)00689-X. doi: 
            10.1016/j.cell.2023.06.013.
  PUBMED    37437570
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Jul 10:S0092-8674(23)00689-X. doi: 10.1016/j.cell.2023.06.013."
COMMENT     Sequence Label: pFH2.106_PB-EPN24-MCP_I-T2A-eGFP-PGK-Blast
FEATURES             Location/Qualifiers
     source          1..9018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   14..48
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        612..860
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     612..632
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     783..802
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        942..1153
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1166..1434
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2632..3018
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             3019..3048
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3058..3111
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3112..3114,3115..3828)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3112..3825
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3154..3175)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3415..3434)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3762..3783
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3956..4077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3993..4012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4047..4066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4450..4469
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4547..4556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4553..4951
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4969..5193
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5620..5682
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6181..6197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6181..6197)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6194..6216)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6205..6221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6229..6235,6236..6253,6254..6259))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6274..6295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6412..6429)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6583..7171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6663..6682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7342..8136)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7342..8136)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7453..7472)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8063..8082
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8137..8241)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8267..8722)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8404..8425)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8616..8635
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8849..8871
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8863..8880
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8864..8880
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcagctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
       61 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      121 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      181 ataacaaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      241 actcatacga taattatatt gttatttcgt gttctactta cgtgataact tattatatat
      301 atattttctt gttatagata tccttgaggg acagcccccc cccaaagccc ccagggatgt
      361 aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc
      421 ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg
      481 tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg
      541 gggggatacg gggaaaaatc gattaactat aacggtccta aggtagcgaa cacctgcgct
      601 cgagcgacgt cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
      661 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
      721 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      781 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      841 tgtggaaagg acgaaacacc gaccaggtgg gcccacgcgt ttttttcacc tgcattaccc
      901 tgttatccct aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc
      961 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg
     1021 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     1081 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     1141 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc
     1201 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc
     1261 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg
     1321 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct
     1381 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct
     1441 gtgaccggcg cctacgctag cggatccgcc accatgcacg gtctgcagga cgacccggac
     1501 ctgcaagcac tgctcaaggg cagccagctg ctgaaagtta aatcttcttc ttggcgtcgt
     1561 gaacgtttct acaaactcca ggaggactgc aaaaccatct ggcaggaatc ccgtaaagtt
     1621 atgcgttctc cggaatctca gctgttctct atcgaagaca tccaggaagt tcgtatgggt
     1681 caccgtaccg aaggtctgga aaaattcgcg cgtgacatcc cggaagaccg ttgcttctcc
     1741 atcgttttca aagaccagcg taacaccctg gacctgatcg cgccgtctcc ggcggacgcg
     1801 cagcactggg ttcagggtct gcgtaaaatc atccaccact ctggttctat ggaccaacgt
     1861 cagaaactgc agtctcgtcc ggaaccgacc gcaccgccgg aagaatcttt ccgttctggt
     1921 gttgaaacca ccacgccacc gcaaaaacag gagccgatcg acaaagaact gtacccgctg
     1981 acctctctgc gttctctgtt cggtaacgac ccgtcttctc agaagatcga agagctgttc
     2041 aagaaacaca agatcgttgc cgtgctgcgt gccaatagtg tggaagaagc gaaaaagaaa
     2101 gcgctggcgg ttttcctggg cggcgttcat ctgattgaaa ttacctttac cgtgccggat
     2161 gcggataccg tgattaagga actgagcttt ctgaaggaaa tgggcgcgat tattggtgcg
     2221 ggcaccgtga ccagcgtgga gcagtgccgt aaagcggtgg aaagtggcgc cgagttcatt
     2281 gtgagtccgc acctggacga ggaaattagc caattttgca aggagaaggg tgtgttctat
     2341 atgccaggcg ttatgacccc gaccgaactg gtgaaagcca tgaaactggg ccataccatc
     2401 ttaaaactgt ttccgggtga ggtggtgggt ccgcagtttg ttaaagcgat gaaaggtccg
     2461 tttccgaatg tgaaatttgt gccaaccggc ggtgttaatc tggacaatgt gtgcgaatgg
     2521 ttcaaagcgg gcgtgctggc cgtgggcgtg ggcagcgcgt tagtgaaagg caccccggtg
     2581 gaagtggcgg aaaaggccaa ggcgttcgtt gagaagattc gtggctgcac cgcttcaaac
     2641 tttactcagt tcgtgctcgt ggacaatggt gggacagggg atgtgacagt ggctccttct
     2701 aatttcgcta atggggtggc agagtggatc agctccaact cacggagcca ggcctacaag
     2761 gtgacatgca gcgtcaggca gtctagtgcc cagaagagaa agtataccat caaggtggag
     2821 gtccccaaag tggctaccca gacagtgggc ggagtcgaac tgcctgtcgc cgcttggagg
     2881 tcctacctga acatggagct cactatccca attttcgcta ccaattctga ctgtgaactc
     2941 atcgtgaagg caatgcaggg gctcctcaaa gacggtaatc ctatcccttc cgccatcgcc
     3001 gctaactcag gtatctacga acagaagctg atctccgaag aagacctggg atcgggagag
     3061 ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc aatgagcaag
     3121 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3181 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     3241 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3301 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     3361 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     3421 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     3481 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3541 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     3601 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     3661 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     3721 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3781 gtgaccgccg ccgggatcac tcacggcatg gacgagctgt acaagtaagc ggccgcgtcg
     3841 acgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     3901 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3961 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4021 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagc
     4081 gccggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     4141 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     4201 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     4261 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     4321 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     4381 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     4441 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     4501 catctccggg cctttcgacc tgcagcccaa gcttaccatg ggtaccgcca ccatggccaa
     4561 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     4621 ccccatctct gaagattaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     4681 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     4741 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     4801 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     4861 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     4921 gctgccctct ggttatgtgt gggagggcta aggtcacccg cgcgcggact gtgccttcta
     4981 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     5041 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     5101 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     5161 gcaggcatgc tggggatgcg gtgggctcta tggttcgaac cagtacgatg gttttccccg
     5221 tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc
     5281 ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg
     5341 ggagcgccgg accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg
     5401 acgtaattac atccctgggg gctttggggg ggggctgtcc ctgataaaag ttttgttact
     5461 ttatagaaga aattttgagt ttttgttttt ttttaataaa taaataaaca taaataaatt
     5521 gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa tatctattca
     5581 aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat tttacgcatg
     5641 attatcttta acgtacgtca caatatgatt atctttctag ggttaatcta gctgcggcgc
     5701 gccatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct
     5761 atctgggcat cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga
     5821 aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca
     5881 tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca
     5941 cctgggatac cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc
     6001 gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta
     6061 atgacagcgg tgcggcgctg ggatattacg tcagcgagga cgggtatcct ggctggatgc
     6121 cgcagaaatg gacatggata ccccgtgagt tacccggcgg gcgcgcttgg cgtaatcatg
     6181 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6241 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6301 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6361 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     6421 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6481 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7021 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7261 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7321 tgagtaaact tggtctgaca gtcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     7381 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     7441 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     7501 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     7561 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct
     7621 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     7681 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     7741 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     7801 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     7861 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     7921 cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag
     7981 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     8041 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     8101 cggagaacct gcgtgcaatc catcttgttc aatcatactc ttcctttttc aatattattg
     8161 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8221 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     8281 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     8341 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     8401 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     8461 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     8521 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     8581 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     8641 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     8701 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     8761 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     8821 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     8881 gagcgcgcct cgttcattca cgtttttgaa cccgtggagg acgggcagac tcgcggtgca
     8941 aatgtgtttt acagcgtgat ggagcagatg aagatgctcg acacgctgca gaacacgagc
     9001 tccaccgcgg ggcgcgcc
//
