LOCUS       sequence-408288-        7560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human UGCG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Horns F, Martinez JA, Fan C, Haque M, Linton JM, Tobin V, Santat L, 
            Maggiolo AO, Bjorkman PJ, Lois C, Elowitz MB
  TITLE     Engineering RNA export for measurement and manipulation of living 
            cells.
  JOURNAL   Cell. 2023 Jul 10:S0092-8674(23)00689-X. doi: 
            10.1016/j.cell.2023.06.013.
  PUBMED    37437570
REFERENCE   2  (bases 1 to 7560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Jul 10:S0092-8674(23)00689-X. doi: 10.1016/j.cell.2023.06.013."
COMMENT     Sequence Label: pJAM1.21_PB-UGCG-PGK-Blast
FEATURES             Location/Qualifiers
     source          1..7560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   14..48
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        660..871
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             884..1152
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      1180..1189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2498..2619
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2535..2554)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2589..2608
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2992..3011
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3089..3098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3095..3493
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3511..3735
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4162..4224
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4723..4739)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4723..4739)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4736..4758)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4747..4763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4771..4777,4778..4795,4796..4801))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4816..4837
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4954..4971)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5125..5713)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5205..5224)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5884..6678)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5884..6678)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5995..6014)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6605..6624
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6679..6783)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6809..7264)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6946..6967)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7158..7177
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7391..7413
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7405..7422
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7406..7422
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcagctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
       61 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      121 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      181 ataacaaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      241 actcatacga taattatatt gttatttcgt gttctactta cgtgataact tattatatat
      301 atattttctt gttatagata tccttgaggg acagcccccc cccaaagccc ccagggatgt
      361 aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc
      421 ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg
      481 tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg
      541 gggggatacg gggaaaaatc gattaactat aacggtccta aggtagcgaa cacctgcgct
      601 cgagcgacgt cattaccctg ttatccctag gatctgcgat cgctccggtg cccgtcagtg
      661 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      721 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      781 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      841 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
      901 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
      961 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     1021 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     1081 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     1141 ccgttacaga tccaagctgt gaccggcgcc tacgctagcg ccaccatggc gctgctggac
     1201 ctggccttgg agggaatggc cgtcttcggg ttcgtcctct tcttggtgct gtggctgatg
     1261 catttcatgg ctatcatcta cacccgatta cacctcaaca agaaggcaac tgacaaacag
     1321 ccttatagca agctcccagg tgtctctctt ctgaaaccac tgaaaggggt agatcctaac
     1381 ttaatcaaca acctggaaac attctttgaa ttggattatc ccaaatatga agtgctcctt
     1441 tgtgtacaag atcatgatga tccagccatt gatgtatgta agaagcttct tggaaaatat
     1501 ccaaatgttg atgctagatt gtttataggt ggcaaaaaag ttggcattaa tcctaaaatt
     1561 aataatttaa tgccaggata tgaagttgca aagtatgatc ttatatggat ttgtgatagt
     1621 ggaataagag taattccaga tacgcttact gacatggtga atcaaatgac agaaaaagta
     1681 ggcttggttc acgggctgcc ttacgtagca gacagacagg gctttgctgc caccttagag
     1741 caggtatatt ttggaacttc acatccaaga tactatatct ctgccaatgt aactggtttc
     1801 aaatgtgtga caggaatgtc ttgtttaatg agaaaagatg tgttggatca agcaggagga
     1861 cttatagctt ttgctcagta cattgccgaa gattacttta tggccaaagc gatagctgac
     1921 cgaggttgga ggtttgcaat gtccactcaa gttgcaatgc aaaactctgg ctcatattca
     1981 atttctcagt ttcaatccag aatgatcagg tggaccaaac tacgaattaa catgcttcct
     2041 gctacaataa tttgtgagcc aatttcagaa tgctttgttg ccagtttaat tattggatgg
     2101 gcagcccacc atgtgttcag atgggatatt atggtatttt tcatgtgtca ttgcctggca
     2161 tggtttatat ttgactacat tcaactcagg ggtgtccagg gtggcacact gtgtttttca
     2221 aaacttgatt atgcagtcgc ctggttcatc cgcgaatcca tgacaatata catttttttg
     2281 tctgcattat gggacccaac tataagctgg agaactggtc gctacagatt acgctgtggg
     2341 ggtacagcag aggaaatcct agatgtatag gcggccgcgt cgacgactct agatcataat
     2401 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     2461 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     2521 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2581 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa gcgccggcgc tacacaagtg
     2641 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     2701 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     2761 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     2821 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     2881 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     2941 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     3001 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     3061 cctgcagccc aagcttacca tgggtaccgc caccatggcc aagcctttgt ctcaagaaga
     3121 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagatta
     3181 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata
     3241 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
     3301 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc
     3361 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa
     3421 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt
     3481 gtgggagggc taaggtcacc cgcgcgcgga ctgtgccttc tagttgccag ccatctgttg
     3541 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3601 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     3661 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     3721 cggtgggctc tatggttcga accagtacga tggttttccc cgtatccccc caggtgtctg
     3781 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt
     3841 gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc
     3901 ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg
     3961 gggctttggg ggggggctgt ccctgataaa agttttgtta ctttatagaa gaaattttga
     4021 gtttttgttt ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat
     4081 tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc
     4141 gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt
     4201 cacaatatga ttatctttct agggttaatc tagctgcggc gcgccatcag gatcatatcg
     4261 tcgggtcttt tttccggctc agtcatcgcc caagctggcg ctatctgggc atcggggagg
     4321 aagaagcccg tgccttttcc cgcgaggttg aagcggcatg gaaagagttt gccgaggatg
     4381 actgctgctg cattgacgtt gagcgaaaac gcacgtttac catgatgatt cgggaaggtg
     4441 tggccatgca cgcctttaac ggtgaactgt tcgttcaggc cacctgggat accagttcgt
     4501 cgcggctttt ccggacacag ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca
     4561 ataccggcga cagccggaac tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc
     4621 tgggatatta cgtcagcgag gacgggtatc ctggctggat gccgcagaaa tggacatgga
     4681 taccccgtga gttacccggc gggcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     4741 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4801 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     4861 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     4921 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4981 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5041 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5101 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5161 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5221 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5281 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5341 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5401 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5461 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5521 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5581 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5641 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5701 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5761 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5821 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5881 cagtcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     5941 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     6001 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     6061 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
     6121 cacgacgaga tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg
     6181 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
     6241 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
     6301 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
     6361 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
     6421 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
     6481 ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
     6541 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
     6601 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
     6661 tccatcttgt tcaatcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6721 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6781 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     6841 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     6901 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     6961 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     7021 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     7081 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     7141 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     7201 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     7261 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     7321 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     7381 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt
     7441 cacgtttttg aacccgtgga ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg
     7501 atggagcaga tgaagatgct cgacacgctg cagaacacga gctccaccgc ggggcgcgcc
//
