LOCUS       sequence-408312-       12736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Whitford CM, Gockel P, Faurdal D, Gren T, Sigrist R, Weber T
  TITLE     CASCADE-Cas3 enables highly efficient genome engineering in 
            Streptomyces species.
  JOURNAL   Nucleic Acids Res. 2025 Mar 20;53(6):gkaf214. doi: 
            10.1093/nar/gkaf214.
  PUBMED    40138716
REFERENCE   2  (bases 1 to 12736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-03-20"; volume: "53(6):gkaf214. doi"; 
            pages: " 10.1093/nar/gkaf214"
COMMENT     Sequence Label: pCRISPR-Cas3
FEATURES             Location/Qualifiers
     source          1..12736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            467..576
                     /note="incP origin of transfer"
     CDS             892..1692
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      1994..2582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2483..2502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2736..2753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2870..2891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2906..2911,2912..2929,2930..2936)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2944..2960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2949..2971
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2968..2984
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2968..2984
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(3345..3379)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(9334..9382)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             11098..12540
                     /codon_start=1
                     /gene="rep"
                     /product="replication initiator protein from the 
                     Streptomyces ghanaensis plasmid pSG5"
                     /label=pSG5 Rep
                     /translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
                     AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
                     IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFRDALAGAWAAGVESAGGYTV
                     SETCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVAKIGLELA
                     GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
                     RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
                     PGVRRLVESWGLVWGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
                     ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
                     APVLQPWGRLPGDCLRVDTAVA"
ORIGIN
        1 ggccgtcgac gacgacgcgt ccgcctgcct gcttttggcc ggcgggccgg gccgcggttg
       61 tccgccgggt cggaccggtc ccttggtcga ccgatgcctc ggccacgacg atgctgggcc
      121 cgaagcggta cgcgaacccg tgccgggctg cggcggatcg cggcagcagg agctccccgc
      181 ccgccacggc tggctggatg gtcgcctcac gcgcccgcag gggtgacggc gtgggtgcgg
      241 cggtcagccc cgtgatcggg cgtcagacgg ccgctgaggg accgcgacca cccctccctt
      301 cccgcctgcc gtctttcacc gcccggccgg tccgcagacg ccgtacggcc aggccacgga
      361 ggattacggg cagatggcgc ccacccggca cccgggctgg ggcaccctgg acagcacgaa
      421 ggcccgcccc actcaccggg gcgggccttc gcgttgagct agagttcccg ccagcctcgc
      481 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
      541 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
      601 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
      661 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa tgcagctcac
      721 ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa
      781 aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt
      841 atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca
      901 gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt
      961 ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc
     1021 cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctgc gctgggtccg
     1081 ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc
     1141 acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat
     1201 gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc
     1261 atctctgatc cattgcccct gccacctcac tcgcctgcaa gcccggtcgc ccgtgtccat
     1321 gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat
     1381 cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag
     1441 gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc
     1501 ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc
     1561 tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga
     1621 gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc
     1681 cagtcgattg gctgagctca taagttccta ttccgaagtt cctattcgaa atgaccgacc
     1741 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     1801 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     1861 tgctggagtt cttcgcccac cccaaaagga tctaggtgaa gatccttttt gataatctca
     1921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2101 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     2401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2821 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     2881 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     2941 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattgt
     3001 acgcggtcga tcttgacggc tggcgagagg tgcggggagg atctgaccga cgcggtccac
     3061 acgtggcacc gcgatgctgt tgtgggcaca atcgtgccgg ttggtaggat cgacgggtcg
     3121 cgccccgcac gggcgcgtgg attgaaacca tggtcgcccg gcaaaaccgg gcgtggattg
     3181 aaacgtaggc gtcagctacg atgttccggg gactgctgat ccggtcagca ggtggaagag
     3241 ggactggatt ccaaagttct caatgctgct tgctgttctt gaatgggggg tcgttgacga
     3301 cgacatggct cgattggcgc gacaagttgc tgcgattctc accaataaaa aacgcccggc
     3361 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggaccggatc
     3421 ggggatctgg gctgagggag ccgacggcac gcggcggctc acggcgtggc acgcggaacg
     3481 tccgggcttg cacctcacgt cacgtgagga ggcagcgtgg acggcgtcag agaagggagc
     3541 ggacatatgg acgccgaggc gtccgacacc cacttcttcg cccactccac cctgaaggcc
     3601 gaccggagcg actggcagcc cctggtggag cacctgcagg ccgtggcgcg cctggccggc
     3661 gagaaggccg cgttcttcgg cggcggcgag ctggccgcgc tggccggcct gctgcacgac
     3721 ctgggcaagt acaccgacga gttccagcgt cgcatcgccg gcgacgcgat ccgggtcgac
     3781 cactccaccc gcggcgccat cctggcggtg gagcggtacg gcgccctggg ccagctgctg
     3841 gcctacggca tcgcgggcca ccacgccggc ctggcgaacg gccgggaggc cggcgagcgc
     3901 accgcgctgg tcgaccggct gaagggcgtg ggcctgcccc gcctgctgga gggctggtgc
     3961 gtggagatcg tgctgcccga gcgcctgcag ccgcccccgc tgaaggcccg gctggagcgg
     4021 ggcttcttcc agctggcctt cctgggccgg atgctgttct cgtgcctggt cgacgccgac
     4081 tacctggaca ccgaggcctt ctaccaccgg gtggagggcc gtcgcagcct gcgggagcag
     4141 gcccgcccca ccctggcgga gctgcgggcc gcgctggacc gccacctgac cgagttcaag
     4201 ggcgacaccc cggtcaaccg cgtgcggggc gagatcctgg ccggcgtccg cggcaaggcg
     4261 tccgagctgc cgggcctgtt ctcgctgacc gtgcccaccg gcggcggcaa gaccctggcc
     4321 agcctggcct tcgccctgga ccacgccctg gcgcacggcc tgcgtcgcgt catctacgtg
     4381 atccccttca cctccatcgt cgagcagaac gccgccgtgt tccgtcgcgc cctgggcgcg
     4441 ctgggcgagg aagccgtcct ggagcaccac agcgccttcg tggacgaccg tcgccagtcc
     4501 ctggaggcca agaagaagct gaacctggcg atggagaact gggacgcccc catcgtggtg
     4561 accaccgccg tccagttctt cgagagcctg ttcgccgacc ggcccgccca gtgccgcaag
     4621 ctgcacaaca tcgccggctc cgtggtgatc ctggacgagg cccagaccct gcccctgaag
     4681 ctgctgcggc cctgcgtggc cgccctggac gagctggccc tgaactaccg ctgctccccg
     4741 gtgctgtgca ccgccaccca gccggccctg cagtcccccg acttcatcgg cggcctgcag
     4801 gacgtccggg agctggcccc ggagccccag cggctgttcc gcgagctggt ccgcgtgcgc
     4861 atccgcaccc tgggccccct ggaggacgcc gccctgaccg agcagatcgc ccgtcgcgag
     4921 caggtcctgt gcatcgtgaa caaccgtcgc caggcccgtg ccctgtacga gtccctggcc
     4981 gagctgcccg gcgcccgcca cctgaccacc ctgatgtgcg ccaagcaccg gtcctcggtc
     5041 ctggcggagg tgcgccagat gctgaagaag ggcgagccct gccgcctggt ggccacctcc
     5101 ctgatcgagg ccggcgtgga cgtggacttc cccgtggtgc tgcgggccga ggccggcctg
     5161 gactcgatcg cccaggccgc gggccggtgc aaccgcgagg gcaagcgccc cctggccgag
     5221 tccgaggtcc tggtgttcgc cgcggccaac agcgactggg caccgccgga ggagctgaag
     5281 cagttcgccc aggccgcccg ggaggtcatg cgcctgcacc ccgacgactg cctgtcgatg
     5341 gcggccatcg agcggtactt ccgcatcctg tactggcaga agggcgccga ggagctggac
     5401 gccggcaacc tgctgggcct gatcgagcgc ggtcgcctgg acggcctgcc ctacgagacc
     5461 ctggccacca agttccgcat gatcgactcc ctgcagctgc ccgtcatcat cccgttcgac
     5521 gacgaggccc gtgccgccct gcgcgagctg gagttcgccg acggctgcgc cgccatcgcc
     5581 cgtcgcctgc agccctacct ggtccagatg ccgcggaagg gctaccaggc cctgcgggag
     5641 gccggcgcca tccaggccgc cgcaggcacc cggtacggcg agcagttcat ggccctggtc
     5701 aaccccgacc tgtaccacca ccagttcggc ctgcactggg acaaccccgc cttcgtgagc
     5761 tccgagcgcc tgtgctggta gtcgggacgc gcaacagcgg cctggcctgg atgatgtgaa
     5821 agggagggcc gatggcctac ggcatccgcc tgatggtctg gggcgagcgg gcctgcttca
     5881 cccggcccga gatgaaggtc gagcgcgtga gctacgacgc catcaccccc tccgccgcgc
     5941 ggggcatcct ggaggccatc cactggaagc ccgccatccg ctgggtggtg gaccgcatcc
     6001 aggtcctgaa gcccatccgc ttcgagtcca tccgtcgcaa cgaggtgggc ggcaagctgt
     6061 ccgccgtgtc cgtgggcaag gccatgaagg ccggccggac caacggcctg gtcaacctgg
     6121 tggaggaaga ccggcagcag cgggccacca ccctgctgcg cgacgtctcg tacgtgatcg
     6181 aggcgcactt cgagatgacc gaccgggccg gcgccgacga caccgtcggc aagcacctgg
     6241 acatcttcaa ccgtcgcgcc cgcaagggcc agtgcttcca caccccctgc ctgggcgtcc
     6301 gggagttccc ggcctcgttc cggctgctgg aggaaggctc cgccgagccg gaggtggacg
     6361 cgttcctgcg cggcgagcgg gacctgggct ggatgctgca cgacatcgac ttcgccgacg
     6421 gcatgacccc gcacttcttc cgggccctga tgcgggacgg cctgatcgag gtgcccgcct
     6481 tccgggccgc ggaggacaag gcatgatcct gtccgccctg aacgactact accagcgcct
     6541 gctggagcgg ggcgaggcca acatctcccc cttcggctac tcgcaggaga agatcagcta
     6601 cgccctgctg ctgtccgccc agggcgagct gctggacgtc caggacatcc ggctgctgtc
     6661 cggcaagaag ccccagcccc ggctgatgag cgtcccccag ccggagaagc gcacctccgg
     6721 catcaagagc aacgtgctgt gggacaagac cagctacgtg ctgggcgtgt ccgccaaggg
     6781 cggcgagcgg acccagcagg agcacgagtc cttcaagacc ctgcaccgcc agatcctggt
     6841 gggcgagggc gacccgggcc tgcaggccct gctgcagttc ctggactgct ggcagccgga
     6901 gcagttcaag ccgcccctgt tctcggaggc catgctggac agcaacctgg tgttccggct
     6961 ggacggccag cagcggtacc tgcacgagac ccccgccgcg ctggccctgc gcacccggct
     7021 gctggcggac ggcgactccc gcgagggcct gtgcctggtc tgcggccagc gccagccgct
     7081 ggcccggctg caccccgcgg tcaagggcgt gaacggcgcc cagtcctccg gcgcgtccat
     7141 cgtgtcgttc aacctggacg ccttcagctc ctacggcaag agccagggcg agaacgcccc
     7201 cgtctccgag caggccgcct tcgcctacac caccgtgctg aaccacctgc tgcgtcgcga
     7261 cgagcacaac cggcagcggc tgcagatcgg cgacgcctcc gtggtgttct gggcccaggc
     7321 cgacaccccg gcccaggtgg ccgccgcaga gtcgaccttc tggaacctgc tggagcctcc
     7381 ggccgacgac ggccaggaag ccgagaagct gcggggcgtc ctggacgccg tggccaccgg
     7441 ccgcccgctg cacgagctgg actcgctgat ggaggaaggc acccgcatct tcgtgctggg
     7501 cctggccccc aacaccagcc gcctgtccat ccgcttctgg gccgtggaca gcctggcggt
     7561 gttcacccag cacctggccg agcacttccg cgacatgcac ctggagcccc tgccctggaa
     7621 gaccgagccc gccatctggc gcctgctgta cgccaccgcc ccctcccgcg acggccgggc
     7681 caagaccgag gacctgctgc cccagctggc cggcgagatg acccgggcga tcctgaccgg
     7741 ctcccgctac ccccggtccc tgctggccaa cctgatcatg cggatgcggg ccgacggcga
     7801 cgtcagcggc atccgcgtgg ccctgtgcaa ggccgtcctg gcccgcgagg cccggctgtc
     7861 cggcaagatc caccaggaag agctgccgat gtccctggac aaggacgcct cgaaccccgg
     7921 ctaccgcctg ggccggctgt tcgccgtcct ggagggcgcc cagcgggccg ccctgggcga
     7981 ccgggtgaac gccaccatcc gcgaccggta ctacggcgcc gcctcctcca cccccgcgac
     8041 cgtcttcccc atcctgctgc ggaacaccca gaaccacctg gccaagctgc gcaaggagaa
     8101 gcccggcctg gcggtgaacc tggagcggga catcggcgag atcatcgacg gcatgcagtc
     8161 gcagttcccg cgctgcctgc ggctggagga ccagggccgc ttcgccatcg gctactacca
     8221 gcaggcgcag gcccgcttca accggggccc ggactccgtc gagtaaggag cagaagaatg
     8281 accgccatca gcaaccgcta cgagttcgtc tacctgttcg acgtgtccaa cggcaacccc
     8341 aacggcgacc ccgacgccgg caacatgccc cggctggacc ccgagaccaa ccagggcctg
     8401 gtcaccgacg tgtgcctgaa gcgcaagatc cggaactacg tctccctgga gcaggagtcc
     8461 gcccccggct acgcgatcta catgcaggag aagtcggtgc tgaacaacca gcacaagcag
     8521 gcctacgagg cgctgggcat cgagagcgag gccaagaagc tgcccaagga cgaggccaag
     8581 gcgcgcgagc tgacctcctg gatgtgcaag aacttcttcg acgtccgggc cttcggcgcc
     8641 gtgatgacca ccgagatcaa cgccggccag gtccggggcc ccatccagct ggccttcgcg
     8701 accagcatcg acccggtcct gccgatggag gtgtccatca cccggatggc cgtcaccaac
     8761 gagaaggacc tggagaagga gcgcacgatg ggccggaagc acatcgtgcc ctacggcctg
     8821 taccgggccc acggcttcat ctccgccaag ctggcggagc ggaccggctt cagcgacgac
     8881 gacctggagc tgctgtggcg ggccctggcg aacatgttcg agcacgaccg gtccgccgcg
     8941 cggggcgaga tggccgcgcg gaagctgatc gtcttcaagc acgagcacgc gatgggcaac
     9001 gcccccgccc acgtgctgtt cggctccgtc aaggtggagc gggtggaggg cgacgccgtg
     9061 acccccgccc gcggtttcca ggactaccgg gtctccatcg acgccgaggc gctgccccag
     9121 ggcgtctcgg tgcgcgagta cctgtagaag ctttctagag gatccccggg taccgagctc
     9181 gaattcccca gatctaaagt tttgtcgtct ttccagacgt tagtaaatga attttctgta
     9241 tgaggttttg ctaaacaact ttcaacagtt tcagcggagt gagaatagaa aggaacaact
     9301 aaaggaattg cgaataataa ttttttcacg ttgaaaatct ccaaaaaaaa aggctccaaa
     9361 aggagccttt aattgtatcg gtttatcagc ttgctttcga ggtgaatttc ttaaacagct
     9421 tgataccgat agttgcgccg acaatgacaa caaccatcgc ccacgcataa ccgatatatt
     9481 cggtcgctga ggcttgcagg gagtcaaagg ccgcttttgc gggatctcgt cgaaggcact
     9541 agaaggccta tatgcgggga tcgaccgcgc gggtcccgga cggggaagag cggggagctt
     9601 tgccagagag cgacgacttc cccttgcgtt ggtgattgcc ggtcagggca gccatccgcc
     9661 atcgtcgcgt agggtgtcac accccaggaa tcgcgtcact gaacacagca gccggtagga
     9721 cgaccatgac tgagttggac accatcgcaa atccgtccga tcccgcggtg cagcggatca
     9781 tcgatgtcac caagccgtcg cgatccaaca taaagacaac gttgatcgag gacgtcgagc
     9841 ccctcatgca cagcatcgcg gccggggtgg agttcatcga ggtctacggc agcgacagca
     9901 gtccttttcc atctgagttg ctggatctgt gcgggcggca gaacataccg gtccgcctca
     9961 tcgactcctc gatcgtcaac cagttgttca agggggagcg gaaggccaag acattcggca
    10021 tcgcccgcgt ccctcgcccg gccaggttcg gcgatatcgc gagccggcgt ggggacgtcg
    10081 tcgttctcga cggggtgaag atcgtcggga acatcggcgc gatagtacgc acgtcgctcg
    10141 cgctcggagc gtcggggatc atcctggtcg acagtgacat caccagcatc gcggaccggc
    10201 gtctccaaag ggccagccga ggttacgtct tctcccttcc cgtcgttctc tccggtcgcg
    10261 aggaggccat cgccttcatt cgggacagcg gtatgcagct gatgacgctc aaggcggatg
    10321 gcgacatttc cgtgaaggaa ctcggggaca atccggatcg gctggccttg ctgttcggca
    10381 gcgaaaaggg tgggccttcc gacctgttcg aggaggcgtc ttccgcctcg gtttccatcc
    10441 ccatgatgag ccagaccgag tctctcaacg tttccgtttc cctcggaatc gcgctgcacg
    10501 agaggatcga caggaatctc gcggccaacc gataagcgcc tctgttcctc ggacgctcgg
    10561 ttcctcgacc tcgattcgtc agtgatgatc aacaggaccc agaaatggca cgagcccggg
    10621 attggcctcc cgggctcgcc gcccccgaac cggcgcagca tgccgagtga ggacgtttgc
    10681 tctgtgacga gcgtacgaga gaacagcgcg tcgacaccac cgcgccccgc cggaccgaag
    10741 tgccactgcg gcaccccgat cgtccacatc cccgggaagc gccccaaaat ctactgctcg
    10801 gacgcctgcc gaaagcgcgc aaagcgtctc attgcggacc agtcacgcag cgtagcaagc
    10861 acgactacag cgggtaatcc ggacgcgggc tttagtagta agggaaaatc tccgtccact
    10921 gcaggtcaga gcgctgtaac tggaacgggt tctaccccaa acgagggtgc cgcgacagcc
    10981 gggcgggagg acccgcgcga cgagcggttc gcccggcggg accggcacca gacggtgtcg
    11041 ctgaacgagg cgttcaaggg ctgcgggacc cggctcactg ccgggacggc cgagctgatg
    11101 tggaagccgg gcgaggcgac gtggggcaac acgtgccgct gcaacaacgt ccacacctgc
    11161 ccgtggtgca tgagccgcat cctggccgta cggggctcga acgtgcagct cgccgccgac
    11221 ggtctcgcgg acgccgggta cgggctgcac cttggcacga acacgttgcg ccacttcgag
    11281 cgcatggcgt tcggcaccgt ccgcaaggga atgcggcacg gcctggtcgc ggtcttgcac
    11341 gacggctgga agggcgcgta cggctcgtcc ggccgccgct ggcgcacgat gcgcgacgac
    11401 ttcgggatca tcggctacga gcgggccttc gaggacacct tcggctgggg ctccggctgg
    11461 cacctgcact ggcacacgct ctgggtcacc cgcgaggtgc tcggcccgga cgcccaggcc
    11521 gcgttccgcg acgcactcgc cggagcctgg gccgccgggg tcgaatccgc gggcggctac
    11581 accgtcagcg agacgtgcga ccggcccggt tgctcctgtg agggcaaggg ccacggcacc
    11641 gacgtgcgcc cactcaacgg cgcggacgcc gctgacggcg atgcgggcaa gcaggcccgc
    11701 tacctctaca aggacggcga caagaccaag ggcggcgtcg ccaagatcgg cctggagctg
    11761 gccggacaga acttcaaggc cggtcgcggc gacgaccgca tgggaccgct cgacctgggc
    11821 gacgcggcgg ccgccgagct gcagcggctt cgtcggcccg gcccgttcgt cgagaagtac
    11881 cgcgagcgcg agttcggcgt cttccaggtc cgcaagcact accggtcgca gaacctgaac
    11941 cggctgatca aggaactcgg catccagcag gacgtccgca ccgaggagga gatcaccgac
    12001 gacaccgagg gcctggtcgc catcgcggtc atcccggcct acatctggta ccgatacatc
    12061 gcccgcgtcg ccggacgtcg cctcgacctc atcaaggtcg ccgagacgta cggcctaccg
    12121 ggcgtccggc ggctcgtcga gtcctggggc ctggtgtggg gcaaggacgt cctggacccg
    12181 cccgccccgg aagcgccggc cgcgccgggc gaccttgacg ctgaccagat gaggttcgag
    12241 gtcatgtccg aggaggaagc tgctttccgc gaggcgcgcc ggaaggccaa cgaggcccgc
    12301 acggaggagc tggccgcttc gctcgaccga gtgcgtcagc cgaagaagga ggcgatccgc
    12361 ccgacgatca gccttcggaa gcgcctgaag ccgaagccgg tcaccgtcga cgtcaagact
    12421 cccccgcccg gcgccgcgtc gcccgtgtgc cgccgctgca agggcaagct cgccccggta
    12481 ctccagccct ggggccggct ccccggagac tgcctccgcg tcgacacagc ggtcgcgtag
    12541 ggccgctgag ggaccgccat gagaccgcca ccccggacat ccgggtggcg gtctcatggc
    12601 ggtcccggcg cgaaagccga gggtctgcct gccgtgaggt ggccggcgga cctgggttac
    12661 tgctggaatc ccgcgcccag tccgagcgtc cgccggcgct tttcacctgg ctccgggtgc
    12721 tcgtcgatga gcacca
//
