LOCUS       sequence-408323-        8086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pnmr-1 FLPe unc-54 3' UTR C.elegans AVA/E/D premotor IN and others 
            expression of FLPe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Meng J, Ahamed T, Yu B, Hung W, Ei Mouridi S, Wang Z, Zhang Y, Wen 
            Q, Boulin T, Gao S, Zhen M
  TITLE     A tonically active master neuron modulates mutually exclusive motor 
            states at two timescales.
  JOURNAL   Sci Adv. 2024 Apr 12;10(15):eadk0002. doi: 10.1126/sciadv.adk0002. 
            Epub 2024 Apr 10.
  PUBMED    38598630
REFERENCE   2  (bases 1 to 8086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-04-12"; volume: "10(15):eadk0002. doi"; pages: " 
            10.1126/sciadv.adk0002. Epub 2024 Apr 10"
COMMENT     Sequence Label: pJH2673
FEATURES             Location/Qualifiers
     source          1..8086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3250..4521
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFGILCKTPPKVLVRQFVERFERPSGEKIASCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     primer_bind     complement(5526..5548)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5648..5667
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5745..5763)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5831..5935
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5936..6004,6005..6796)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6154..6173)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6967..7555
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7456..7475
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7709..7726
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7843..7864
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7879..7884,7885..7902,7903..7909)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7917..7933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7922..7944
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7941..7957
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7941..7957
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcatgc ggccgctgtg gtctcacttt gaacgacaaa agaaaaccaa
       61 atattgtaaa ggaatagtac agataaaagg gaacaagtga taccagctga tggatgtagg
      121 tcagcgacaa aagacaggag acgtatagtt tgatgattat ggaaccaaac tcagaattta
      181 atgttggaga cgctataaaa attgaattga gaaattgaaa ccatattctt gaatatctct
      241 caatttggaa tttggaagta gccgtaaaat cggctagcta gaacataaaa ggactgattt
      301 gcaaccttga ccattcatga tatcatttca ataaaagaag agattccaat gaccaatctt
      361 caagaatcac aataatcaca acaaaactaa gcaaaagtaa caacaaattg ttcaggtaga
      421 caagtagaca tttggacaca ttttgcgggg atgtgatgac tgtataataa aaataagtgg
      481 acactgagag agagaggagg tcagcaggaa aaaagaacat cgaactgctt gacgaattga
      541 aattagaatg aggtgtcagg caggtgacga ttacatggtt aattattagt gttctttttc
      601 ttctttttgt ggatgccgat tagttgaaat aattgcatgg aaagtgagaa ttcaatgtga
      661 atctctttct tattatgttt caagtggtat ctggatggaa gcttgtactt ttatgaatcg
      721 aaaattgcgc ccaaggttga ttttgagatt attcagtaaa tttttgtgct gattattcag
      781 ttaatgtaga acagagacgc ggatgaaaac ttttttaatt tctaaaaaaa aagacaaaat
      841 gtggttaaaa agtttggaaa ataattgtta tatacttctt gagagcttat tcaaacggaa
      901 accaatcata aatagtcgaa tagtcattta gaaaagagaa actagttgat gtagagagat
      961 tcaaaaagta aaatatgcaa atgatccgtt tcttagattt agtaaacatc taccaaatga
     1021 aacaggaatt gtcgaaacaa tttagaaaag gagaaggaaa catttgttta ttttcccctt
     1081 taaatggcgg tagaggtaaa acagcaatac acatagtaca aggttatact actttcaaaa
     1141 gaatttgaca tcccgccgag aaaatgtggt gattatatgg taaagttttc agttggatat
     1201 tatcttctac agacggtgat tcaaaatgat caaaagggca agtgggtggt aggatttgaa
     1261 aaattgagat atagaacgtg cgattttggt aaaacttttt gaacacaagg catatttttg
     1321 aactttgaaa aacgcaaaat ttaaattttg ttaattacat atattttgaa cagattatga
     1381 atataagcaa ggctacataa aatatgtata ttttcttttt acaaatattc gtacactgtt
     1441 aaatttatag aaaaatcatt tatattcaga aaaacctatt tgaacaattt ttagttgctc
     1501 aataaccaat ttgcacaaat ttagaaaata taatgggggc ttttaaaaat ttaaatcctc
     1561 agcgaatatt cctttgcgat tttttcgaac gttttctttc agattaaaaa atctgaagca
     1621 gaagtaaatg aactaccata gaaaattcac caaggatcac atttttacaa agcctccgcc
     1681 caattgtaaa cttgggaatt tttgcttgtt ttcttttttt tttcaaaaaa ggaatagttg
     1741 ttttaaagta atttattccc ctatgctttc tccagcaatc agttgttctc cccatacttt
     1801 acacaattat caacaacttc acatagaaaa caaaacaaca aatcagaaat tcccaccggt
     1861 cacagtcaca gattccccat gttttgttca tctcatttct gcaagaaagt gacatcgctg
     1921 ccacccaatt cgacaaattg tccaagtggt catcaggaag atatgacctt ctttttaatg
     1981 ataatttaga ctacaaaggg cgtcaaaaaa gattcaatga tcaggggtaa ttgctgtaaa
     2041 atgtagtttg tatgtgtgtt taccgtagcc tataactgta aaaattggtt ttttaaaaag
     2101 tatttctgag attaaaaggc tccccaagga tgcacgattc tagctagaaa tgaacattcc
     2161 atgggttgtg aagatgcttt tatctcactt tagaatgtct gactccgccc ctctgtgtac
     2221 gaattgaagt gctcttttgt gatgatcata gctcctcctt gcggcgacgc ccgcacacag
     2281 cacgagatat tacacggaat acttatgtga ttcataggaa acaaggaaat cgaaaaaata
     2341 ataggatttt gaggaggtat ttttagatga atgcttatga tgttatgatg aacccaagaa
     2401 attctgaata gacatatgtc agaacggggt aggagctcca gtgtcgcttt tgaaataaaa
     2461 aatcaagtgg tatacttctc gtatataccc ttctgaacta ataaaataat ttatcttttg
     2521 aaaaagtttg aaaaagaaaa atgcccaaaa cttgaatttt aatgttttgt attttgagca
     2581 tatcgttcaa acgtcgtgat ctgaaagtag aaagatctaa atattcgtga cacagtttgg
     2641 tttgaaggtc ttagtggtaa ttttttatta cagaagcaac aaaaagttga catattttca
     2701 gaatttccaa aaaaacgttt aaaatttcaa tttcttcgat agagttctaa aaattttttg
     2761 gaacggattt tgtaatatcc gctgataacc gttcaagaac aatttgaatt ttgaaataat
     2821 ttaatcaact agtatccagt gctctttctc actgctccac ccatttttca acccatttcc
     2881 tttactcaat ttcgcccgtt ttccttttgc tcaaatgtgc ccaccaaatc atctttcttc
     2941 gtcttctttt ctcttattcg gaaggagaag aggtgaaagt agaataagtt tttttgttga
     3001 aaagagagga gcacggccac ttttctctta tttccatcct tctactacct cgttttactt
     3061 tccatttaaa aacccgttta aagttccaaa atttcaatta tcttgcttct gtttggaaca
     3121 cgacaaactt tagttttgtt acagataccc gggattggcc aaaggaccca aaggtatgtt
     3181 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggcta gcgtcgacgg
     3241 atccaaaaaa tgccacaatt tggtatatta tgtaaaacac cacctaaggt cctggttcgt
     3301 cagtttgtgg aaaggtttga aagaccttca ggggaaaaaa tagcatcatg tgctgctgaa
     3361 ctaacctatt tatgttggat gattactcat aacggaacag caatcaagag agccacattc
     3421 atgagctata atactatcat aagcaattcg ctgagtttcg atattgtcaa caaatcactc
     3481 cagtttaaat acaagacgca aaaagcaaca attctggaag cctcattaaa gaaattaatt
     3541 cctgcttggg aatttacaat tattccttac aatggacaaa aacatcaatc tgatatcact
     3601 gatattgtaa gtagtttgca attacagttc gaatcatcgg aagaagcaga taagggaaat
     3661 agccacagta aaaaaatgct taaagcactt ctaagtgagg gtgaaagcat ctgggagatc
     3721 actgagaaaa tactaaattc gtttgagtat acctcgagat ttacaaaaac aaaaacttta
     3781 taccaattcc tcttcctagc tactttcatc aattgtggaa gattcagcga tattaagaac
     3841 gttgatccga aatcatttaa attagtccaa aataagtatc tgggagtaat aatccagtgt
     3901 ttagtgacag agacaaagac aagcgttagt aggcacatat acttctttag cgcaaggggt
     3961 aggatcgatc cacttgtata tttggatgaa tttttgagga attctgaacc agtcctaaaa
     4021 cgagtaaata ggaccggcaa ttcttcaagc aacaaacagg aataccaatt attaaaagat
     4081 aacttagtca gatcgtacaa caaggctttg aagaaaaatg cgccttatcc aatctttgct
     4141 ataaagaatg gcccaaaatc tcacattgga agacatttga tgacctcatt tctgtcaatg
     4201 aagggcctaa cggagttgac taatgttgtg ggaaattgga gcgataagcg tgcttctgcc
     4261 gtggccagga caacgtatac tcatcagata acagcaatac ctgatcacta cttcgcacta
     4321 gtttctcggt actatgcata tgatccaata tcaaaggaaa tgatagcatt gaaggatgag
     4381 actaatccaa ttgaggagtg gcagcatata gaacagctaa agggtagtgc tgaaggaagc
     4441 atacgatacc ccgcatggaa tgggataata tcacaggagg tactagacta cctttcatcc
     4501 tacataaata gacgcatata aggatccccg agatcttcag cgctcagcat tcgtagaatt
     4561 gtaagtttaa actttctcat actaactaac gcgctctatt taaattttca gaattccaac
     4621 tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag gggccgctgt
     4681 catcagagta agtttaaact gagttctact aactaacgag taatatttaa attttcagca
     4741 tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct
     4801 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt
     4861 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga
     4921 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc
     4981 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat
     5041 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     5101 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg
     5161 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga
     5221 aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg
     5281 acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa
     5341 gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag
     5401 tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa
     5461 ctgtttccta ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt
     5521 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     5581 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     5641 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     5701 cacagatgcg taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct
     5761 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5821 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5881 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5941 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6001 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6061 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6121 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6181 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6241 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6301 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     6361 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     6421 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     6481 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6541 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6601 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6661 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6721 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6781 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6841 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6901 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6961 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7021 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7081 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7141 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7201 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7261 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7321 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7381 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7441 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7501 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7561 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7621 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7681 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7741 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     7801 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     7861 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     7921 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctgtaagt
     7981 ttaaacatga tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct
     8041 gaaatcactc acaacgatgg atacgctaac aacttggaaa tgaaat
//
