LOCUS       sequence-408324-        8317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pcex-1 tomm-20::miniSOG UrSL mCherry unc-54 3' UTR C.elegans RIM 
            neuron expression of miniSOG RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   Meng J, Ahamed T, Yu B, Hung W, Ei Mouridi S, Wang Z, Zhang Y, Wen 
            Q, Boulin T, Gao S, Zhen M
  TITLE     A tonically active master neuron modulates mutually exclusive motor 
            states at two timescales.
  JOURNAL   Sci Adv. 2024 Apr 12;10(15):eadk0002. doi: 10.1126/sciadv.adk0002. 
            Epub 2024 Apr 10.
  PUBMED    38598630
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-04-12"; volume: "10(15):eadk0002. doi"; pages: " 
            10.1126/sciadv.adk0002. Epub 2024 Apr 10"
COMMENT     Sequence Label: pJH2829
FEATURES             Location/Qualifiers
     source          1..8317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3220..3540
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(5757..5779)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5879..5898
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5976..5994)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6062..6166
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6167..6235,6236..7027)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6385..6404)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7198..7786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7687..7706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7940..7957
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8074..8095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8110..8115,8116..8133,8134..8140)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8148..8164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8153..8175
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8172..8188
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8172..8188
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcatgc ggccgccacc aacctggaaa actacacatt ctatggggaa
       61 tatcaatcag gatgtttatt ggaaatataa ttttaaaaaa cttgaaaatt gttactacga
      121 attctgtaca tttttaattt ttggggttga agatgcagta aaaatttttg gtgttgattt
      181 ttcagtttct ataaaatggc gctgaagaat tgaaatgtaa aagcttgaat agtattatgc
      241 aacaccgatg cagagctcag aattaatgag cactactttg agaataggtg tactgtattt
      301 ggatttcaag atttatacaa ctaaaccaac catatttcca acttgtttcc tctccatatg
      361 aaaattaata atatcatatt cgactccaat tcgttcacat ttatcattaa aatgcacacg
      421 actagttgat gttccacata tttcatttaa tagagcaggt gcttgaaggg aattccattc
      481 agcatctaca ctatctcgat cacattctac tggcgggaag atatctattt tcgattctcg
      541 aaatatagtt tccatggcag atttcagaat tgaaaatgaa tctcttaaaa ctgaaaatgc
      601 agtttgtccg agtcgacatc caagtgatcc atttggaaca ttatgagctt caccagcact
      661 ttcactcacg atccatactt ttccttttcc agtaagatcg ccagcatttg caaatatacg
      721 tactgcatcg tcttttctaa aatcttatgt tattgaagtt tgttcgaggt cgcaaacttt
      781 gcaaaaagca caatgatatt agatgtaact tcctcgaaac tctcagccaa tgtgtcattc
      841 aagttctcgt tcagctctac ctcaatgtaa cgttgaacct ggaaaatgtt ttagatctca
      901 aaacttgtat tgtctaaaaa tcaactcaca attattttga attcaactcg ccgtttttca
      961 aactcttcca caaattgatc ggcatttata tctcttttca cacttaatac cacaacctaa
     1021 atataaaaca tttcaattct atgtatcttc attctagatt tacctgtcga tatttcaagc
     1081 ttaatagcat atgtagaaat acaaatgctt catctgaaag tggagccgtt ggacgaacaa
     1141 atgtgggata gatattctaa attgaacttt taaaacaata ttagtcaatt tatttaacga
     1201 tcaccttttt actaaactct gcatctctaa ccattactcc aacaactgga agtttgtaaa
     1261 atgaaagtgt atatgctgat gatgtaacca aatcaataaa tcttgaagat gatgtcagta
     1321 atggagaaaa tataacagaa gccaatgagt gattgagcat ctggctgcac acaatatcct
     1381 gagtcaattc cagacgatta tctccactga atataagaaa tgtctttctc gggggaaaag
     1441 ctcaacaaat aataatttcg ataacctttt tgcatctaca aaagctgcgg aaatagtgtc
     1501 attccccaag tgatatgatg tgcttccaaa aacttccacg aatgccgcct gaaagaaatt
     1561 aagaagaaaa gttgaaaata tgatattttg tgaatatgaa tatgctaatt taaaagtttg
     1621 gagcttcttc tataatgccg agtttgtaag tttttaaagt aaaaatttca aaacttaccg
     1681 taacggaaac tttcaattct ttgaaagttt cttgattcgg ctcagaaaca attaaaactg
     1741 atactttata gtctattgtg tatcccaatt gtaaaaatat ccaaataaat ataactgata
     1801 ttcggaacat atctgtaaca aaactaaagt ttgtcgtgtt ccaaacagaa gcaagataat
     1861 tgaaattttg gaactttaaa cgggttttta aatggaaagt aaaacgaggt agtagaagga
     1921 tggaaataag agaaaagtgg cagtgctctt ctcttttcaa caaaaaaacg tattctactt
     1981 tcacctcttc tccttccgaa taagagaaaa gaagacgaag aaagatgatt tggtgggcac
     2041 atttgagcaa aaggaaaacg ggcgaaattg agtaaaggaa atgggttgaa aaatgggtgg
     2101 agcagtgaga aagagcactg gatactagtt gattaattta tttcaaaatt caaattgttc
     2161 ttgaacggtt atcagcggat attacaaaat ccgttccaaa aaatttttag aactctatcg
     2221 aagaaattga aattttaaac gtttttttgg aaattctgaa aatatgtcaa ctttttgttg
     2281 cttctgtaat aaaaaattac cactaagacc ttcaaaccaa actgtgtcac gaatatttag
     2341 atctttctac tttcagatca cgacgtttga acgatatgct caaaatacaa aacattaaaa
     2401 ttcaagtttt gggcattttt ctttttcaaa ctttttcaaa agataaatta ttttattagt
     2461 tcagaagggt atatacgaga agtataccac ttgatttttt atttcaaagc gacactggag
     2521 ctcctacccc gttctgacat atgtctattc agaatttctt gggttcatca taacatcata
     2581 agcattcatc taaaaatacc tcctcaaaat cctattattt tttcgatttc cttgtttcct
     2641 atgaatcaca taagtattcc gtgtaatatc tcgtgctgtg tgcgggcgtc gccgcaagga
     2701 ggagctatga tcatcacaaa agagcacttc aattcgtaca cagaggggcg gagtcagaca
     2761 ttctaaagtg agataaaagc atcttcacaa cccatggaat gttcatttct agatattttt
     2821 tcttgatttc tagactttct caagttcata attttataat tttcagtcaa atcttatact
     2881 ttcagaaatc ccgggattgg ccaaaggacc caaaggtatg tttcgaatga tactaacata
     2941 acatagaaca ttttcaggag gacccttggc tagcgtcgac ggatcccgat gtcggacaca
     3001 attcttggtt tcaacaaatc aaacgtcgtt ttggctgctg gaattgctgg agccgctttc
     3061 ctcggctact gcatttactt cgatcataag agaatcaacg ctccagacta caaggacaag
     3121 attaggcaaa gtcagtgttt taacaacata tttccttcgg atttttatct aaaaacaact
     3181 tattttcttt cagagagacg tgcccaggct ggggatccta tggagaaaag tttcgtgata
     3241 actgatccac ggctgccaga caatcccatc atcttcgcat ccgatggctt cctggagctg
     3301 accgagtatt ccagagagga gatcctgggc cgcaatggcc gctttctgca gggaccagag
     3361 acagaccagg ccacagtgca gaagattcgc gatgccatta gagatcagcg cgagattacc
     3421 gtgcagctga taaactacac aaaaagcggg aagaaattct ggaacctcct gcacctccag
     3481 cccatgaggg accagaaggg tgagctccag tatttcatcg gagtgcagct ggatggataa
     3541 agatccccga gatcttcagc gctcagcatt cgtagaattg taagtttaaa ctttctcata
     3601 ctaactaacg cgctctattt aaattttcag aattcgctgt ctcatcctac tttcacctag
     3661 ttaactgctt gtcttaaaat ctatgcttct ctttagtatc taaaattttc ctagaagctt
     3721 acaagtatat aaatggtctc ttctcaataa aggttgtata tttattcatc ttattgaatc
     3781 tgccatttcc tcgtttttgc gagtttatat accttccaat tttctttcta ttgtattttc
     3841 aacttctaat tttaattcag ggaaactgct tcaacgcatc ggtaccctat ggtctcaaag
     3901 ggtgaagaag ataacatggc aattattaaa gagtttatgc gtttcaaggt gcatatggag
     3961 ggatctgtca atgggcatga gtttgaaatt gaaggtgaag gagaaggccg accatatgag
     4021 ggaacacaaa ccgcaaaact aaaggtaagt ttaaacatat atatactaac taaccctgat
     4081 tatttaaatt ttcaggtaac taaaggcgga ccattaccat tcgcctggga catcctctct
     4141 ccacagttca tgtatggaag taaagcttat gttaaacatc cggcagatat accagattat
     4201 ttgaaacttt cattcccgga gggttttaag tgggaacgcg taatgaattt tgaagacgga
     4261 ggagttgtta cagtgacgca agactcaagg taagtttaaa cagttcggta ctaactaacc
     4321 atacatattt aaattttcag cctccaagat ggagaattta tttataaagt caaacttcga
     4381 ggaacgaatt tcccctcgga tggatctgtt atgcagaaga agactatggg atgggaagct
     4441 tcaagtgaaa gaatgtaccc tgaagacggt gctcttaagg gagagattaa acaacgtctt
     4501 aaattgaaag atggaggaca ttacgatgct gaggtaagtt taaacatgat tttactaact
     4561 aactaatctg atttaaattt tcaggtgaag acaacttaca aagccaaaaa accagttcag
     4621 ctgccaggag cgtacaatgt taatattaaa ctggatatca cctcccacaa cgaggattac
     4681 actatcgttg agcaatatga aagagctgaa gggcggcact cgacaggtgg catggatgaa
     4741 ttgtataagt aggagctccc gagatcttca gcgctcagca ttcgtagaat tgtaagttta
     4801 aactttctca tactaactaa cgcgctctat ttaaattttc agaattccaa ctgagcgccg
     4861 gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt
     4921 aagtttaaac tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc
     4981 cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg
     5041 tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta
     5101 gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg
     5161 taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa
     5221 atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa
     5281 acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac
     5341 cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg
     5401 aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt
     5461 tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt
     5521 ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     5581 ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     5641 tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct
     5701 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     5761 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     5821 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     5881 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     5941 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
     6001 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     6061 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6121 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6181 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6241 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6301 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6361 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6421 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6481 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6541 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6601 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6661 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6721 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6781 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6841 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6901 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6961 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7021 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7081 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7141 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7201 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7261 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7321 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7381 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7441 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7501 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7561 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7621 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7681 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7741 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7801 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7861 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7921 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7981 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8041 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8101 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8161 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctgtaag tttaaacatg
     8221 atcttactaa ctaactattc tcatttaaat tttcagagct taaaaatggc tgaaatcact
     8281 cacaacgatg gatacgctaa caacttggaa atgaaat
//
