LOCUS       sequence-408326-        9546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express EPN24-MCP-IRES-eGFP and blasticidin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Horns F, Martinez JA, Fan C, Haque M, Linton JM, Tobin V, Santat L, 
            Maggiolo AO, Bjorkman PJ, Lois C, Elowitz MB
  TITLE     Engineering RNA export for measurement and manipulation of living 
            cells.
  JOURNAL   Cell. 2023 Jul 10:S0092-8674(23)00689-X. doi: 
            10.1016/j.cell.2023.06.013.
  PUBMED    37437570
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Jul 10:S0092-8674(23)00689-X. doi: 10.1016/j.cell.2023.06.013."
COMMENT     Sequence Label: pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast
FEATURES             Location/Qualifiers
     source          1..9546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   14..48
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        612..860
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     612..632
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     783..802
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        942..1153
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1166..1434
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2632..2661
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2662..3051
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     misc_feature    3058..3631
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3225..3242)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3452..3471
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3640..3642,3643..4356)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3640..4353
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3682..3703)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3943..3962)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4290..4311
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4484..4605
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4521..4540)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4575..4594
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4978..4997
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      5075..5084
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5081..5479
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5497..5721
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6148..6210
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6709..6725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6709..6725)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6722..6744)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6733..6749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6757..6763,6764..6781,6782..6787))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6802..6823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6940..6957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7111..7699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7191..7210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7870..8664)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7870..8664)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7981..8000)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8591..8610
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8665..8769)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8795..9250)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8932..8953)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9144..9163
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9377..9399
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9391..9408
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9392..9408
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcagctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
       61 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      121 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      181 ataacaaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      241 actcatacga taattatatt gttatttcgt gttctactta cgtgataact tattatatat
      301 atattttctt gttatagata tccttgaggg acagcccccc cccaaagccc ccagggatgt
      361 aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc
      421 ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg
      481 tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg
      541 gggggatacg gggaaaaatc gattaactat aacggtccta aggtagcgaa cacctgcgct
      601 cgagcgacgt cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
      661 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
      721 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      781 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      841 tgtggaaagg acgaaacacc gaccaggtgg gcccacgcgt ttttttcacc tgcattaccc
      901 tgttatccct aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc
      961 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg
     1021 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     1081 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     1141 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc
     1201 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc
     1261 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg
     1321 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct
     1381 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct
     1441 gtgaccggcg cctacgctag cggatccgcc accatgcacg gtctgcagga cgacccggac
     1501 ctgcaagcac tgctcaaggg cagccagctg ctgaaagtta aatcttcttc ttggcgtcgt
     1561 gaacgtttct acaaactcca ggaggactgc aaaaccatct ggcaggaatc ccgtaaagtt
     1621 atgcgttctc cggaatctca gctgttctct atcgaagaca tccaggaagt tcgtatgggt
     1681 caccgtaccg aaggtctgga aaaattcgcg cgtgacatcc cggaagaccg ttgcttctcc
     1741 atcgttttca aagaccagcg taacaccctg gacctgatcg cgccgtctcc ggcggacgcg
     1801 cagcactggg ttcagggtct gcgtaaaatc atccaccact ctggttctat ggaccaacgt
     1861 cagaaactgc agtctcgtcc ggaaccgacc gcaccgccgg aagaatcttt ccgttctggt
     1921 gttgaaacca ccacgccacc gcaaaaacag gagccgatcg acaaagaact gtacccgctg
     1981 acctctctgc gttctctgtt cggtaacgac ccgtcttctc agaagatcga agagctgttc
     2041 aagaaacaca agatcgttgc cgtgctgcgt gccaatagtg tggaagaagc gaaaaagaaa
     2101 gcgctggcgg ttttcctggg cggcgttcat ctgattgaaa ttacctttac cgtgccggat
     2161 gcggataccg tgattaagga actgagcttt ctgaaggaaa tgggcgcgat tattggtgcg
     2221 ggcaccgtga ccagcgtgga gcagtgccgt aaagcggtgg aaagtggcgc cgagttcatt
     2281 gtgagtccgc acctggacga ggaaattagc caattttgca aggagaaggg tgtgttctat
     2341 atgccaggcg ttatgacccc gaccgaactg gtgaaagcca tgaaactggg ccataccatc
     2401 ttaaaactgt ttccgggtga ggtggtgggt ccgcagtttg ttaaagcgat gaaaggtccg
     2461 tttccgaatg tgaaatttgt gccaaccggc ggtgttaatc tggacaatgt gtgcgaatgg
     2521 ttcaaagcgg gcgtgctggc cgtgggcgtg ggcagcgcgt tagtgaaagg caccccggtg
     2581 gaagtggcgg aaaaggccaa ggcgttcgtt gagaagattc gtggctgcac cgaacagaag
     2641 ctgatctccg aagaagacct ggcttcaaac tttactcagt tcgtgctcgt ggacaatggt
     2701 gggacagggg atgtgacagt ggctccttct aatttcgcta atggggtggc agagtggatc
     2761 agctccaact cacggagcca ggcctacaag gtgacatgca gcgtcaggca gtctagtgcc
     2821 cagaagagaa agtataccat caaggtggag gtccccaaag tggctaccca gacagtgggc
     2881 ggagtcgaac tgcctgtcgc cgcttggagg tcctacctga acatggagct cactatccca
     2941 attttcgcta ccaattctga ctgtgaactc atcgtgaagg caatgcaggg gctcctcaaa
     3001 gacggtaatc ctatcccttc cgccatcgcc gctaactcag gtatctacta agaattcccc
     3061 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     3121 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     3181 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     3241 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     3301 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     3361 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     3421 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     3481 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     3541 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     3601 cgtggttttc ctttgaaaaa cacgatgata acctgcagga tgagcaaggg cgaggagctg
     3661 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3721 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3781 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     3841 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3901 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     3961 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4021 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     4081 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     4141 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     4201 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     4261 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4321 gggatcactc acggcatgga cgagctgtac aagtaagcgg ccgcgtcgac gactctagat
     4381 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     4441 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     4501 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4561 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaagcgc cggcgctaca
     4621 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     4681 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     4741 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     4801 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     4861 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     4921 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     4981 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     5041 tttcgacctg cagcccaagc ttaccatggg taccgccacc atggccaagc ctttgtctca
     5101 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     5161 agattacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     5221 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     5281 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     5341 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     5401 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     5461 ttatgtgtgg gagggctaag gtcacccgcg cgcggactgt gccttctagt tgccagccat
     5521 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5581 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5641 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     5701 gggatgcggt gggctctatg gttcgaacca gtacgatggt tttccccgta tccccccagg
     5761 tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt
     5821 ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac
     5881 cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat
     5941 ccctgggggc tttggggggg ggctgtccct gataaaagtt ttgttacttt atagaagaaa
     6001 ttttgagttt ttgttttttt ttaataaata aataaacata aataaattgt ttgttgaatt
     6061 tattattagt atgtaagtgt aaatataata aaacttaata tctattcaaa ttaataaata
     6121 aacctcgata tacagaccga taaaacacat gcgtcaattt tacgcatgat tatctttaac
     6181 gtacgtcaca atatgattat ctttctaggg ttaatctagc tgcggcgcgc catcaggatc
     6241 atatcgtcgg gtcttttttc cggctcagtc atcgcccaag ctggcgctat ctgggcatcg
     6301 gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa gagtttgccg
     6361 aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg atgattcggg
     6421 aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc tgggatacca
     6481 gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc atcagcaacc
     6541 cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat gacagcggtg
     6601 cggcgctggg atattacgtc agcgaggacg ggtatcctgg ctggatgccg cagaaatgga
     6661 catggatacc ccgtgagtta cccggcgggc gcgcttggcg taatcatggt catagctgtt
     6721 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6781 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6841 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6901 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6961 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7021 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7081 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7141 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7201 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7261 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7321 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7381 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7441 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7501 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7561 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7621 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7681 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7741 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7801 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7861 gtctgacagt cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     7921 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     7981 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     8041 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     8101 atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc
     8161 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     8221 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     8281 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     8341 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     8401 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     8461 cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac
     8521 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     8581 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     8641 gtgcaatcca tcttgttcaa tcatactctt cctttttcaa tattattgaa gcatttatca
     8701 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8761 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     8821 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     8881 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     8941 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     9001 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     9061 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     9121 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     9181 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     9241 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     9301 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     9361 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcctcg
     9421 ttcattcacg tttttgaacc cgtggaggac gggcagactc gcggtgcaaa tgtgttttac
     9481 agcgtgatgg agcagatgaa gatgctcgac acgctgcaga acacgagctc caccgcgggg
     9541 cgcgcc
//
