LOCUS       sequence-408405-       12459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human CIT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12459)
  AUTHORS   Horns F, Martinez JA, Fan C, Haque M, Linton JM, Tobin V, Santat L, 
            Maggiolo AO, Bjorkman PJ, Lois C, Elowitz MB
  TITLE     Engineering RNA export for measurement and manipulation of living 
            cells.
  JOURNAL   Cell. 2023 Jul 10:S0092-8674(23)00689-X. doi: 
            10.1016/j.cell.2023.06.013.
  PUBMED    37437570
REFERENCE   2  (bases 1 to 12459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Jul 10:S0092-8674(23)00689-X. doi: 10.1016/j.cell.2023.06.013."
COMMENT     Sequence Label: pJAM1.19_PB-CIT-PGK-Blast
FEATURES             Location/Qualifiers
     source          1..12459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   14..48
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        660..871
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             884..1152
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     polyA_signal    7397..7518
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7434..7453)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7488..7507
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7891..7910
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      7988..7997
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7994..8392
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8410..8634
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   9061..9123
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9622..9638)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9622..9638)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9635..9657)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9646..9662
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9670..9676,9677..9694,9695..9700))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9715..9736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9853..9870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10024..10612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10104..10123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10783..11577)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(10783..11577)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10894..10913)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     11504..11523
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(11578..11682)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11708..12163)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11845..11866)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12057..12076
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12290..12312
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12304..12321
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12305..12321
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcagctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
       61 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      121 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      181 ataacaaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      241 actcatacga taattatatt gttatttcgt gttctactta cgtgataact tattatatat
      301 atattttctt gttatagata tccttgaggg acagcccccc cccaaagccc ccagggatgt
      361 aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc
      421 ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg
      481 tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg
      541 gggggatacg gggaaaaatc gattaactat aacggtccta aggtagcgaa cacctgcgct
      601 cgagcgacgt cattaccctg ttatccctag gatctgcgat cgctccggtg cccgtcagtg
      661 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      721 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      781 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      841 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
      901 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
      961 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     1021 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     1081 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     1141 ccgttacaga tccaagctgt gaccggcgcc tacgctagcg ccaccatgtt gaagttcaaa
     1201 tatggagcgc ggaatccttt ggatgctggt gctgctgaac ccattgccag ccgggcctcc
     1261 aggctgaatc tgttcttcca ggggaaacca ccctttatga ctcaacagca gatgtctcct
     1321 ctttcccgag aagggatatt agatgccctc tttgttctct ttgaagaatg cagtcagcct
     1381 gctctgatga agattaagca cgtgagcaac tttgtccgga agtattccga caccatagct
     1441 gagttacagg agctccagcc ttcggcaaag gacttcgaag tcagaagtct tgtaggttgt
     1501 ggtcactttg ctgaagtgca ggtggtaaga gagaaagcaa ccggggacat ctatgctatg
     1561 aaagtgatga agaagaaggc tttattggcc caggagcagg tttcattttt tgaggaagag
     1621 cggaacatat tatctcgaag cacaagcccg tggatccccc aattacagta tgcctttcag
     1681 gacaaaaatc acctttatct ggtcatggaa tatcagcctg gaggggactt gctgtcactt
     1741 ttgaatagat atgaggacca gttagatgaa aacctgatac agttttacct agctgagctg
     1801 attttggctg ttcacagcgt tcatctgatg ggatacgtgc atcgagacat caagcctgag
     1861 aacattctcg ttgaccgcac aggacacatc aagctggtgg attttggatc tgccgcgaaa
     1921 atgaattcaa acaagatggt gaatgccaaa ctcccgattg ggaccccaga ttacatggct
     1981 cctgaagtgc tgactgtgat gaacggggat ggaaaaggca cctacggcct ggactgtgac
     2041 tggtggtcag tgggcgtgat tgcctatgag atgatttatg ggagatcccc cttcgcagag
     2101 ggaacctctg ccagaacctt caataacatt atgaatttcc agcggttttt gaaatttcca
     2161 gatgacccca aagtgagcag tgactttctt gatctgattc aaagcttgtt gtgcggccag
     2221 aaagagagac tgaagtttga aggtctttgc tgccatcctt tcttctctaa aattgactgg
     2281 aacaacattc gtaactctcc tccccccttc gttcccaccc tcaagtctga cgatgacacc
     2341 tccaattttg atgaaccaga gaagaattcg tgggtttcat cctctccgtg ccagctgagc
     2401 ccctcaggct tctcgggtga agaactgccg tttgtggggt tttcgtacag caaggcactg
     2461 gggattcttg gtagatctga gtctgttgtg tcgggtctgg actcccctgc caagactagc
     2521 tccatggaaa agaaacttct catcaaaagc aaagagctac aagactctca ggacaagtgt
     2581 cacaagatgg agcaggaaat gacccggtta catcggagag tgtcagaggt ggaggctgtg
     2641 cttagtcaga aggaggtgga gctgaaggcc tctgagactc agagatccct cctggagcag
     2701 gaccttgcta cctacatcac agaatgcagt agcttaaagc gaagtttgga gcaagcacgg
     2761 atggaggtgt cccaggagga tgacaaagca ctgcagcttc tccatgatat cagagagcag
     2821 agccggaagc tccaagaaat caaagagcag gagtaccagg ctcaagtgga agaaatgagg
     2881 ttgatgatga atcagttgga agaggatctt gtctcagcaa gaagacggag tgatctctac
     2941 gaatctgagc tgagagagtc tcggcttgct gctgaagaat tcaagcggaa agcgacagaa
     3001 tgtcagcata aactgttgaa ggctaaggat caagggaagc ctgaagtggg agaatatgcg
     3061 aaactggaga agatcaatgc tgagcagcag ctcaaaattc aggagctcca agagaaactg
     3121 gagaaggctg taaaagccag cacggaggcc accgagctgc tgcagaatat ccgccaggca
     3181 aaggagcgag ccgagaggga gctggagaag ctgcagaacc gagaggattc ttctgaaggc
     3241 atcagaaaga agctggtgga agctgaggag ctcgaagaga aacatcggga ggcccaagtc
     3301 tcagcccagc acctagaagt gcacctgaaa cagaaagagc agcactatga ggaaaagatt
     3361 aaagtgttgg acaatcagat aaagaaagac ctggctgaca aggagacact ggagaacatg
     3421 atgcagagac acgaggagga ggcccatgag aagggcaaaa ttctcagcga acagaaggcg
     3481 atgatcaatg ctatggattc caagatcaga tccctggaac agaggattgt ggaactgtct
     3541 gaagccaata aacttgcagc aaatagcagt ctttttaccc aaaggaacat gaaggcccaa
     3601 gaagagatga tttctgaact caggcaacag aaattttacc tggagacaca ggctgggaag
     3661 ttggaggccc agaaccgaaa actggaggag cagctggaga agatcagcca ccaagaccac
     3721 agtgacaaga atcggctgct ggaactggag acaagattgc gggaggtcag tctagagcac
     3781 gaggagcaga aactggagct caagcgccag ctcacagagc tacagctctc cctgcaggag
     3841 cgcgagtcac agttgacagc cctgcaggct gcacgggcgg ccctggagag ccagcttcgc
     3901 caggcgaaga cagagctgga agagaccaca gcagaagctg aagaggagat ccaggcactc
     3961 acggcacata gagatgaaat ccagcgcaaa tttgatgctc ttcgtaacag ctgtactgta
     4021 atcacagacc tggaggagca gctaaaccag ctgaccgagg acaacgctga actcaacaac
     4081 caaaacttct acttgtccaa acaactcgat gaggcttctg gcgccaacga cgagattgta
     4141 caactgcgaa gtgaagtgga ccatctccgc cgggagatca cggaacgaga gatgcagctt
     4201 accagccaga agcaaacgat ggaggctctg aagaccacgt gcaccatgct ggaggaacag
     4261 gtcatggatt tggaggccct aaacgatgag ctgctagaaa aagagcggca gtgggaggcc
     4321 tggaggagcg tcctgggtga tgagaaatcc cagtttgagt gtcgggttcg agagctgcag
     4381 agaatgctgg acaccgagaa acagagcagg gcgagagccg atcagcggat caccgagtct
     4441 cgccaggtgg tggagctggc agtgaaggag cacaaggctg agattctcgc tctgcagcag
     4501 gctctcaaag agcagaagct gaaggccgag agcctctctg acaagctcaa tgacctggag
     4561 aagaagcatg ctatgcttga aatgaatgcc cgaagcttac agcagaagct ggagactgaa
     4621 cgagagctca aacagaggct tctggaagag caagccaaat tacagcagca gatggacctg
     4681 cagaaaaatc acattttccg tctgactcaa ggactgcaag aagctctaga tcgggctgat
     4741 ctactgaaga cagaaagaag tgacttggag tatcagctgg aaaacattca ggttctctat
     4801 tctcatgaaa aggtgaaaat ggaaggcact atttctcaac aaaccaaact cattgatttt
     4861 ctgcaagcca aaatggacca acctgctaaa aagaaaaagg gtttatttag tcgacggaaa
     4921 gaggaccctg ctttacccac acaggttcct ctgcagtaca atgagctgaa gctggccctg
     4981 gagaaggaga aagctcgctg tgcagagcta gaggaagccc ttcagaagac ccgcatcgag
     5041 ctccggtccg cccgggagga agctgcccac cgcaaagcaa cggaccaccc acacccatcc
     5101 acgccagcca ccgcgaggca gcagatcgcc atgtccgcca tcgtgcggtc gccagagcac
     5161 cagcccagtg ccatgagcct gctggccccg ccatccagcc gcagaaagga gtcttcaact
     5221 ccagaggaat ttagtcggcg tcttaaggaa cgcatgcacc acaatattcc tcaccgattc
     5281 aacgtaggac tgaacatgcg agccacaaag tgtgctgtgt gtctggatac cgtgcacttt
     5341 ggacgccagg catccaaatg tctcgaatgt caggtgatgt gtcaccccaa gtgctccacg
     5401 tgcttgccag ccacctgcgg cttgcctgct gaatatgcca cacacttcac cgaggccttc
     5461 tgccgtgaca aaatgaactc cccaggtctc cagaccaagg agcccagcag cagcttgcac
     5521 ctggaagggt ggatgaaggt gcccaggaat aacaaacgag gacagcaagg ctgggacagg
     5581 aagtacattg tcctggaggg atcaaaagtc ctcatttatg acaatgaagc cagagaagct
     5641 ggacagaggc cggtggaaga atttgagctg tgccttcccg acggggatgt atctattcat
     5701 ggtgccgttg gtgcttccga actcgcaaat acagccaaag cagatgtccc atacatactg
     5761 aagatggaat ctcacccgca caccacctgc tggcccggga gaaccctcta cttgctagct
     5821 cccagcttcc ctgacaaaca gcgctgggtc accgccttag aatcagttgt cgcaggtggg
     5881 agagtttcta gggaaaaagc agaagctgat gctaaactgc ttggaaactc cctgctgaaa
     5941 ctggaaggtg atgaccgtct agacatgaac tgcacgctgc ccttcagtga ccaggtggtg
     6001 ttggtgggca ccgaggaagg gctctacgcc ctgaatgtct tgaaaaactc cctaacccat
     6061 gtcccaggaa ttggagcagt cttccaaatt tatattatca aggacctgga gaagctactc
     6121 atgatagcag gagaagagcg ggcactgtgt cttgtggacg tgaagaaagt gaaacagtcc
     6181 ctggcccagt cccacctgcc tgcccagccc gacatctcac ccaacatttt tgaagctgtc
     6241 aagggctgcc acttgtttgg ggcaggcaag attgagaacg ggctctgcat ctgtgcagcc
     6301 atgcccagca aagtcgtcat tctccgctac aacgaaaacc tcagcaaata ctgcatccgg
     6361 aaagagatag agacctcaga gccctgcagc tgtatccact tcaccaatta cagtatcctc
     6421 attggaacca ataaattcta cgaaatcgac atgaagcagt acacgctcga ggaattcctg
     6481 gataagaatg accattcctt ggcacctgct gtgtttgccg cctcttccaa cagcttccct
     6541 gtctcaatcg tgcaggtgaa cagcgcaggg cagcgagagg agtacttgct gtgtttccac
     6601 gaatttggag tgttcgtgga ttcttacgga agacgtagcc gcacagacga tctcaagtgg
     6661 agtcgcttac ctttggcctt tgcctacaga gaaccctatc tgtttgtgac ccacttcaac
     6721 tcactcgaag taattgagat ccaggcacgc tcctcagcag ggacccctgc ccgagcgtac
     6781 ctggacatcc cgaacccgcg ctacctgggc cctgccattt cctcaggagc gatttacttg
     6841 gcgtcctcat accaggataa attaagggtc atttgctgca agggaaacct cgtgaaggag
     6901 tccggcactg aacaccaccg gggcccgtcc acctcccgca gcagccccaa caagcgaggc
     6961 ccacccacgt acaacgagca catcaccaag cgcgtggcct ccagcccagc gccgcccgaa
     7021 ggccccagcc acccgcgaga gccaagcaca ccccaccgct accgcgaggg gcggaccgag
     7081 ctgcgcaggg acaagtctcc tggccgcccc ctggagcgag agaagtcccc cggccggatg
     7141 ctcagcacgc ggagagagcg gtcccccggg aggctgtttg aagacagcag caggggccgg
     7201 ctgcctgcgg gagccgtgag gaccccgctg tcccaggtga acaaggtctg ggaccagtct
     7261 tcagtatagg cggccgcgtc gacgactcta gatcataatc agccatacca catttgtaga
     7321 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     7381 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7441 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7501 actcatcaat gtatcttaag cgccggcgct acacaagtgg cctctggcct cgcacacatt
     7561 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     7621 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     7681 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     7741 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     7801 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     7861 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     7921 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     7981 gggtaccgcc accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     8041 aacggctaca atcaacagca tccccatctc tgaagattac agcgtcgcca gcgcagctct
     8101 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     8161 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     8221 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     8281 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     8341 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct aaggtcaccc
     8401 gcgcgcggac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     8461 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     8521 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     8581 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggttcgaa
     8641 ccagtacgat ggttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga
     8701 agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc
     8761 tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg
     8821 ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc
     8881 cctgataaaa gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa
     8941 ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata
     9001 ataaaactta atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca
     9061 catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta
     9121 gggttaatct agctgcggcg cgccatcagg atcatatcgt cgggtctttt ttccggctca
     9181 gtcatcgccc aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc
     9241 gcgaggttga agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg
     9301 agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg
     9361 gtgaactgtt cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt
     9421 tccggatggt cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact
     9481 gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg
     9541 acgggtatcc tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg
     9601 ggcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     9661 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     9721 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9781 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9841 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9901 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9961 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    10021 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    10081 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    10141 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    10201 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    10261 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    10321 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    10381 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    10441 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    10501 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    10561 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    10621 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    10681 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    10741 taaatcaatc taaagtatat atgagtaaac ttggtctgac agtcagaaga actcgtcaag
    10801 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
    10861 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
    10921 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
    10981 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
    11041 gggcatgctc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
    11101 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
    11161 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
    11221 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
    11281 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
    11341 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag
    11401 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
    11461 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
    11521 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatact
    11581 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    11641 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    11701 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
    11761 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
    11821 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
    11881 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
    11941 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
    12001 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
    12061 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
    12121 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
    12181 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    12241 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    12301 cgttgtaaaa cgacggccag tgagcgcgcc tcgttcattc acgtttttga acccgtggag
    12361 gacgggcaga ctcgcggtgc aaatgtgttt tacagcgtga tggagcagat gaagatgctc
    12421 gacacgctgc agaacacgag ctccaccgcg gggcgcgcc
//
