LOCUS       sequence-408410-       10385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prig-3 FRT let858 (stop) FRT snb-1::GFP unc-54 3' UTR C.elegans AVA 
            and other neurons expression of snb GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Meng J, Ahamed T, Yu B, Hung W, Ei Mouridi S, Wang Z, Zhang Y, Wen 
            Q, Boulin T, Gao S, Zhen M
  TITLE     A tonically active master neuron modulates mutually exclusive motor 
            states at two timescales.
  JOURNAL   Sci Adv. 2024 Apr 12;10(15):eadk0002. doi: 10.1126/sciadv.adk0002. 
            Epub 2024 Apr 10.
  PUBMED    38598630
REFERENCE   2  (bases 1 to 10385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-04-12"; volume: "10(15):eadk0002. doi"; pages: " 
            10.1126/sciadv.adk0002. Epub 2024 Apr 10"
COMMENT     Sequence Label: pJH4169
FEATURES             Location/Qualifiers
     source          1..10385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(4865..4898)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(5380..5413)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(6062..6090)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6838..6858
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(7825..7847)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7947..7966
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8044..8062)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8130..8234
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8235..8303,8304..9095)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8453..8472)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9266..9854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9755..9774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10008..10025
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10142..10163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10178..10183,10184..10201,10202..10208)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10216..10232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10221..10243
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10240..10256
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10240..10256
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggc ggccgctcgt ctttaccgct ttgtttttta ttgcttactt
       61 tacctctagt gattatctta ttggaattta tgcacatgac gtgttgtact agtttaacaa
      121 tttcaattga cagttttttc gattaggctt ttgaacatat ttatatattt ctttatttac
      181 gaatgtgaac ttaaaagtgg attctcgaaa tcttttatcg ggttttttga gcctaaaatg
      241 acccatttat caagatttat caaaaaagct atgacggttt aaaggttttt gattaaaggc
      301 gtgttttttt tagctaaaat ctcaaactct gctaaactat cagtgcccca acgtgaaaaa
      361 acaacatgaa aaaccccaaa atcaaaaagc atcttaatca gccaaaaaca cttcataaat
      421 tgcaaaaata tgcattaaca aatgacaact aaatggaaaa gtactcccga cttttcaaga
      481 tggaaaaatg tgagatcttc gctgaaaata gagttttttt tcaaatgaaa aatttggaat
      541 aaagctcaaa tgtttgtctc aaagttggta attgttgaaa aaaattttga taatttggcg
      601 aatatttgtg aattgaaaca taactatttc ggttttacgc gtacttgtaa aattcaaaat
      661 tttacaagaa attataaaac tttatgagct ttagatggtc aatacgcaaa cattcatcta
      721 gtttatagcc ttacattaaa acattttacc tattcaggtt aattgaacag tatctttaaa
      781 ggtggtgtag tcgaattttt taaaatttct ttattagact caaaattgtc tgaaaacacc
      841 aaatttcata agtaaacttc ttgaaaactt ttcggctcga aaaaatgacc taaaattagt
      901 gaaaatttga aatgtggcca atttatcaag cggctgcaaa caattttttt ttgaaattgt
      961 tgtctgattt tggatataga tgtaaggtat ttaaaaaaaa gatggacgaa gaaaattggt
     1021 aaaaaaattt gacaaatctc ttcgtccatc gactttgaaa gaccttaatc aagtttaaaa
     1081 cgcaagatag tcaatttata tacccaaaat cagatgataa ttcaaaaaaa attgtttcca
     1141 gccgctgcga caatgttaag ttaatttaag gtcatttttc gagcagccat aacttctttt
     1201 ttttaagttt caacaagttt cattataaaa ttcggtgttt tcagacaatt ttgaatataa
     1261 taaagaaata aagaaaaatt cgactacatc acctttaaac taaatttcaa atgtaatttt
     1321 tttttacttt tcaaaatgat tcgttctcgg ttttgttgtg aacatttttt tgctaatacg
     1381 cttacgaaat gttttgtgtg gcaatcattt tgattgaagt tcccggcagc tacaacatat
     1441 ttttagaact agaaaacaat agaacgcaca gaatatccac gttcccatgt tttcaatttc
     1501 tcttttcgaa atcctttaca aaatccgaac gccgttctga ttcagccttg agacattttt
     1561 caaaaaaaaa atcagtgtga gcattatagt tttcagtttt gaatattcac attttgcact
     1621 ttttgcctac tttccgtatt ctgcagtttc cacatcacct tgttacatct ctcaggaagt
     1681 ttctgaaagt gacaccacgc tcacacacga tgaaattcat acccaccaac ctcccttctc
     1741 tgcctccctc aacttcttcc acggaattct atctcctctt atccagttag ggacacatat
     1801 cgagtttcac caaaaataat gagaagtgtg gtcagagaca ccacattgaa ttaactatta
     1861 gttcaaaagt tgcaggtttc ctagtgcaaa aagtttatgt tcccatctac caaacgactc
     1921 accgccaatt gactttgcag tggcaacatt gggaaatgcg acccatcata ttgttcctat
     1981 tgtcatgtac aagctgtccc tgttttgctc gcttaatccg tcaacccctt ccccgttcta
     2041 attagagatg agtgaattac aatagagttg aaacggaaat ctgagtgtct gtagctttcc
     2101 gccgccgctt ttgttacgag atggtagacg tccctttctt ggtgtttatt gtctcacaaa
     2161 ctgtataata aagcaccgtt gtgtaaaagg catttggtat caggaagata aaatgaataa
     2221 aatgcaaatg cggaatcaaa aattgccaaa taattttgac caataaaata tcaaaaataa
     2281 tagaacagaa ataaacaaat aaaactacgg agaaaatgtg attattccat taaacaacgt
     2341 tttaaaccaa ctttattcgt cagtggggaa ggttttggac aaatatcata gtaaaacttg
     2401 ttcgttttaa ttatacataa tcaataatta taggtgttag atccaatggt cgaaatttaa
     2461 aaaaaaatca acattttaga aaatttcggc aatttaccga aatattgact gacaatttta
     2521 catatataag tgttttgaaa tagaatcccc attgacgatg cgccacctta aacagtagaa
     2581 ctgttccaat cagaaactaa ataaatacgg ttattatata tatatatata tatatataac
     2641 ttgtttttat tatgcatttc tcaaaactca ccaaactcaa tctctaattt acggttgttg
     2701 aggaaaatat atttatcaca tttagttgag actctaaaag aagtacaact tttttcttgc
     2761 aattcctcca ccaatccaat ttttcaagag tcaattgctt ggagtcctcg ttttctcgta
     2821 atccgatatt ccccctattt ctgtgatttt attcagtttc gtcctcttac accaaaaatc
     2881 ttcatgactt ttttggtttt ctgcttaatc ttttttttgt ttttaaagtc taccatccgg
     2941 caattttttt tgcaaatggt tgctcagaat ttgacctcag attaaaattt tcaggaactt
     3001 cctggtccca tttcgtcaca tttaataaca aaccaaatcc ttcagcttga gtcgtccaag
     3061 ccaacgcaac cctaattcga ttccacctcc aatttacctt tttgccgttt tcgatcaaat
     3121 tgtgggggct ctttttattt gaaagtcgta aaatggcgcg tccttgtcga aaattgagat
     3181 agctaatggt tccaaataaa atcaaatgca tgaacctagt aggaaaaatc gttttttttt
     3241 tcatcttcca aagaaaaagt ttaagataaa cccaccactc cagcaatcca cctacgcgtt
     3301 tttcaccgga aactgtcatt tgtttttgat gtatgacagt gccagcactg aactaaattc
     3361 ccatttgttt tgttgtggtg ttatctaacc acgtggctct ttctctaaat tttgcgtagg
     3421 ctgcaagatc cgcaatccca ccaaaaaatg gtttctaatg tacttcatac atatcccaca
     3481 tgactcagtg ctccgaaacg cccagcaacc cccgcgtttg tctttacaaa aatgaggcgg
     3541 aaatgggaaa ttttcctgct attgctatgg taaaatactt aagctgtatt gagatttggg
     3601 aaattgaaag ataagatgaa aaatccactc tgtataatta aaaaggttta ttttttttgt
     3661 aatttttgta ccgcaaattt tttcattaac tgatttttct ttattcattc gatggcccag
     3721 tttcagatct tcattatcat gtagtttatt cattcaaact atttgtgaca tcatgatggt
     3781 tgtttaaaaa taatttcgaa tgaagaaagc ttagacctat tataattttc ttttctataa
     3841 aaaatattga ttttgtaaag cgtttggcag tgacagtacc aaaaaatgtt ttaaaatgat
     3901 ttaagaaaat tttttatttt atttgtataa tacgcgtaaa atgagtatac tcgtcatttg
     3961 tcacgaatcg agttctcagt ttgctgtgaa tatcttgtaa tagaaatcga tctcatagtg
     4021 tcacttttga ggcataactt ttcaggttgt tacaagttca ttaacttgtc aagactgggt
     4081 catgttttaa agtgtaactg ttacccatgt accctaaact ttgagacaaa ctcctcaaaa
     4141 cttgtaacca gaatcctatg tagatgactc acaaaaccct gttttcaatt tgaacgaatt
     4201 tcgaaactgg ccaaaatgtt cccctcttgc tcacctaatg cttttaccat ttaaaaagga
     4261 tggtaacaac actctttcga aaatgtaggc ggtccatttt cggtttctca taaccgtctg
     4321 gctctatctc ctagtgcgcg aggagagggc gggcggagta ggaatcgggg gcagcgcact
     4381 catacactct gcccttctct tttgcacaca cagaaaaaga tagatacaga ccaacactta
     4441 ttgttgtcca gtaagcttcc agaaaactat tttcagaatt acaaactaca tttttctaga
     4501 acagtcaatt gattttttac aagattcgag tattctaaca ttagtgaaag tatttagcca
     4561 ctgcaatttt caactgtagg tttcaacttc caaaaaaaat tcctggcatt tctaattgga
     4621 tgaaaaaagg ctatctagac cctccattat ttactcataa tttcatctcg tcctcatttt
     4681 taatcccctc attgatccca tcacaatccg ccaaaatgtt ttttaaacac ttttttcttc
     4741 accagccgta tgtggtcaac tgcctaaaaa tttcacagct atttgtcatt ccttgcagaa
     4801 gaacttcatt cttctttttc gaaaatcccg ggattggcca aggtaagttt aaacagttcg
     4861 gtacgaagtt cctatacttt ctagagaata ggaacttcta acataacata gaacattttc
     4921 agggatgatc gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt
     4981 ttttcctatt atttatttat tctctttgtg ttttttttct tgctttctaa aaaattaatt
     5041 caatccaaat ctaaacattt ttttttctct ttccgtctcc caattcgtat tccgctcctc
     5101 tcatctgaac acaatgtgca agtttattta tcttctcgct ttcatttcat taggacgtgg
     5161 ggggaattgg tggaaggggg aaacacacaa aaggatgatg gaaatgaaat aaggacacac
     5221 aatatgcaac aacattcaat tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat
     5281 atatagagga ggaaggaaaa ctagtaaaaa ataagcaaag aaattaggcg aacgatgaga
     5341 attgtcctcg cttggaggta agtttaaaca gttcggtacg aagttcctat actttctaga
     5401 gaataggaac ttctaactaa ccatacatat ttaaattttc agggctagcg tcgacggatc
     5461 cccatggacg ctcaaggaga tgccggcgca cagggaggaa gtcagggcgg accacgacca
     5521 tccaacaaac gtctccagca gacacaagct caggttgatg aagtcgtcgg aatcatgaag
     5581 gtaagatttt gaaatgtgca attaaaataa caaaaatcgc aatttttgaa atgatcccat
     5641 ttacaattta taagcgtgtt aaattaatct taattcccct ttttgcaata aaatcacgaa
     5701 aaatagtttt caggtgaacg tggaaaaagt tttggagcgt gatcagaagt tgtctcaact
     5761 ggacgaccga gctgacgctc tccaggaagg tgcttcacaa tttgagaaat ctgcggccac
     5821 cctgaagcgc aagtattggt ggaagaacat caaaatgatg atcatcatgt gcgctatcgt
     5881 cgtcattctt attatcatca tcgttttatg ggctggagga aaactgatcc ccgggattgg
     5941 ccaaaggacc caaaggtatg tttcgaatga tactaacata acatagaaca ttttcaggag
     6001 gacccttgct tggagggtac cggtagaaaa aatgagtaaa ggagaagaac ttttcactgg
     6061 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
     6121 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     6181 tggaaaacta cctgttccat gggtaagttt aaacatatat atactaacta accctgatta
     6241 tttaaatttt cagccaacac ttgtcactac tttctgttat ggtgttcaat gcttctcgag
     6301 atacccagat catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt
     6361 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgta agtttaaaca
     6421 gttcggtact aactaaccat acatatttaa attttcaggt gctgaagtca agtttgaagg
     6481 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     6541 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     6601 acaaaagaat ggaatcaaag ttgtaagttt aaacatgatt ttactaacta actaatctga
     6661 tttaaatttt cagaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     6721 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     6781 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     6841 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     6901 gcattcgtag aattccaact gagcgccggt cgctaccatt accaacttgt ctggtgtcaa
     6961 aaataatagg ggccgctgtc atcagagtaa gtttaaactg agttctacta actaacgagt
     7021 aatatttaaa ttttcagcat ctcgcgcccg tgcctctgac ttctaagtcc aattactctt
     7081 caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa
     7141 aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt
     7201 gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc
     7261 cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg
     7321 ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct
     7381 tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac gtctgggcct
     7441 ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg gaatttttca
     7501 atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt ttcccctatt
     7561 gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat tgatgagcac
     7621 gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt gagtagaagt
     7681 tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac agaatacatt
     7741 cccaatatac caaacataac tgtttcctac tagtcggccg tacgggccct ttcgtctcgc
     7801 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     7861 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     7921 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     7981 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcggcctta
     8041 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     8101 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8161 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8221 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8281 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8341 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8401 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8461 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8521 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8581 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8641 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8701 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8761 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8821 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8881 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8941 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9001 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9061 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9121 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9181 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9241 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9301 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9361 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9421 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9481 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9541 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9601 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9661 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9721 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9781 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9841 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9901 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9961 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10021 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10081 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10141 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    10201 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    10261 attacgccaa gctgtaagtt taaacatgat cttactaact aactattctc atttaaattt
    10321 tcagagctta aaaatggctg aaatcactca caacgatgga tacgctaaca acttggaaat
    10381 gaaat
//
