LOCUS       sequence-408414-       17559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer plasmid containing CMV-driven expression 
            cassette encoding PEmax enzyme for prime editing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17559)
  AUTHORS   Bulcaen M, Kortleven P, Liu RB, Maule G, Dreano E, Kelly M, Ensinck 
            MM, Thierie S, Smits M, Ciciani M, Hatton A, Chevalier B, Ramalho 
            AS, Casadevall I Solvas X, Debyser Z, Vermeulen F, Gijsbers R, 
            Sermet-Gaudelus I, Cereseto A, Carlon MS
  TITLE     Prime editing functionally corrects cystic fibrosis-causing CFTR 
            mutations in human organoids and airway epithelial cells.
  JOURNAL   Cell Rep Med. 2024 May 21;5(5):101544. doi: 
            10.1016/j.xcrm.2024.101544. Epub 2024 May 1.
  PUBMED    38697102
REFERENCE   2  (bases 1 to 17559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Med."; date: "2024-05-21"; volume: "5(5):101544. doi"; pages: " 
            10.1016/j.xcrm.2024.101544. Epub 2024 May 1"
COMMENT     Sequence Label: Lenti-CMV-PEmax-IRES-ZeoR
FEATURES             Location/Qualifiers
     source          1..17559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1191..1210)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1424..1443)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1543..1565
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1603..1621)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1689..1793
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1794..1862,1863..2654)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2012..2031)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2825..3413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3314..3333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3567..3584
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3654..3674)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3659..3988
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3839..3974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5122..5187
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5317..5337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5762..5896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5799..5818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5853..5872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6064..6697
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6744..6869
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7362..7595
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7780..7824
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8117..8234
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8293..8596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8597..8800
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8751..8771
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8842..8861
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8842..8860
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8910..8930
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8931..13031
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             13089..13109
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             15210..15230
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             15240..15266
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     misc_feature    15422..15883
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(15478..15495)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     15705..15724
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             15891..16265
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVADEQD"
     misc_feature    16284..16872
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(16337..16357)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(16755..16766)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             17042..17222
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 acacaagagg aaactgagag ggaaagatgt tgaagtaatt ttcccacaat tacagcatcc
       61 gttagttacg actctatgat cttctgacac aaattccatt tactcctcac cctatgactc
      121 agtcgaatat atcaaagtta tggacattat gctaagtaac aaattaccct tttatatagt
      181 aaatactgag tagattgaga gaagaaattg tttgcaaacc tgaatagctt caagaagaag
      241 agaagtgagg ataagaataa cagttgtcat ttaacaagtt ttaacaagta acttggttag
      301 aaagggattc aaatgcataa agcaagggat aaatttttct ggcaacaaga ctatacaata
      361 taaccttaaa tatgacttca aataattgtt ggaacttgat aaaactaatt aaatattatt
      421 gaagattatc aatattataa atgtaattta cttttaaaaa gggaacatag aaatgtgtat
      481 cattagagta gaaaacaatc cttattatca caatttgtca aaacaagttt gttattaaca
      541 caagtagaat actgcattca attaagttga ctgcagattt tgtgttttgt taaaattaga
      601 aagagataac aacaatttga attattgaaa gtaacatgta aatagttcta catacgttct
      661 tttgacatct tgttcaatca ttgatcgaag ttctttatct tggaagaatt tgttccaaag
      721 actctgaaat aaggaaaaca atctattata tagtctcaca cctttgtttt acttttagtg
      781 atttcaattt aataatgtaa atggttaaaa tttattcttc tctgagatca tttcacattg
      841 cagatagaaa acctgagact ggggtaattt ttattaaaat ctaatttaat ctcagaaaca
      901 catctttatt ctaacatcaa tttttccagt ttgatattat catataaagt cagccttcct
      961 catctgcagg ttccacaaca aaaatccaac caactgtgga tcaaaaatat tgggaaaaaa
     1021 ttaaaaatag caatacaaca ataaaaaaat acaaatcaga aaaacagcac agtataacaa
     1081 ctttatttag catttacaat ctattaggta ttataagtaa tctagccaga tcctctacgc
     1141 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     1201 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     1261 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     1321 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     1381 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     1441 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     1501 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1561 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     1621 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1681 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1741 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1801 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1861 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1921 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1981 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2041 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2101 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2161 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2221 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2281 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2341 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2401 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2461 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2521 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2581 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2641 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2701 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2761 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2821 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2881 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2941 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3001 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3061 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3121 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3181 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3241 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3301 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3361 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3421 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3481 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3541 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3601 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     3661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3961 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     4021 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     4081 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     4141 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     4201 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     4261 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     4321 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     4381 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     4441 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     4501 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     4561 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     4621 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     4681 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     4741 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     4801 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     4861 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     4921 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     4981 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     5041 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     5101 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     5161 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     5221 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     5281 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     5341 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     5401 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     5461 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     5521 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     5581 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     5641 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     5701 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     5761 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5881 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     5941 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     6001 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     6061 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     6121 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     6181 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     6241 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     6301 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     6361 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     6421 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     6481 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     6541 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6601 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6661 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     6721 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     6781 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     6841 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     6901 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     6961 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     7021 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     7081 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     7141 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     7201 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     7261 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     7321 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     7381 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     7441 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     7501 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     7561 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     7621 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     7681 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     7741 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     7801 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     7861 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     7921 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     7981 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     8041 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat tcacaaatgg
     8101 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     8161 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     8221 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ggcgataagc
     8281 ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     8341 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     8401 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     8461 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     8521 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     8581 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     8641 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     8701 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     8761 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
     8821 ccgctagaga tccgcggccg ctaatacgac tcactatagg gagagccgcc accatgaaac
     8881 ggacagccga cggaagcgag ttcgagtcac caaagaagaa gcggaaagtc gacaagaagt
     8941 acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
     9001 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
     9061 agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
     9121 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
     9181 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
     9241 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
     9301 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
     9361 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
     9421 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
     9481 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
     9541 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagaaag ctggaaaatc
     9601 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga
     9661 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
     9721 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
     9781 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
     9841 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
     9901 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
     9961 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg
    10021 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
    10081 gcaccgagga actgctcgtg aagctgaaga gagaggacct gctgcggaag cagcggacct
    10141 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
    10201 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
    10261 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
    10321 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
    10381 gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
    10441 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga
    10501 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
    10561 aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
    10621 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
    10681 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
    10741 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
    10801 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
    10861 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
    10921 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
    10981 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
    11041 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
    11101 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
    11161 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
    11221 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
    11281 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
    11341 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
    11401 accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc gtgcctcaga
    11461 gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg
    11521 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc
    11581 agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga
    11641 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
    11701 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
    11761 agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
    11821 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
    11881 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
    11941 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
    12001 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
    12061 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
    12121 caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga
    12181 aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
    12241 tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg
    12301 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
    12361 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
    12421 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
    12481 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
    12541 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
    12601 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
    12661 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact
    12721 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
    12781 ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc
    12841 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
    12901 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
    12961 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
    13021 tgggaggtga ctccggcgga agctctggtg gcagcaagcg gaccgccgac ggctctgaat
    13081 tcgagagccc taagaagaaa agaaaggtga gcggaggctc tagcggcgga agcaccctga
    13141 acattgaaga cgagtataga ctgcatgaaa caagcaagga acccgacgtg tccctgggct
    13201 ccacctggct gtccgacttt ccccaggcct gggccgagac aggaggaatg ggcctggccg
    13261 tgcggcaggc acccctgatc atccctctga aggccacctc tacacccgtg agcatcaagc
    13321 agtaccctat gtctcaggag gccagactgg gcatcaagcc tcacatccag aggctgctgg
    13381 accagggcat cctggtgcca tgccagagcc cctggaacac accactgctg cccgtgaaga
    13441 agccaggcac caatgactat agacccgtgc aggatctgag agaggtgaac aagagggtgg
    13501 aggatatcca ccccaccgtg cccaaccctt acaatctgct gtccggcctg cccccttctc
    13561 accagtggta tacagtgctg gacctgaagg atgccttctt ttgtctgaga ctgcacccta
    13621 ccagccagcc actgttcgcc tttgagtgga gggaccctga gatgggcatc tctggccagc
    13681 tgacctggac acgcctgcct cagggcttca agaatagccc aacactgttt aacgaggccc
    13741 tgcaccgcga cctggcagat ttccggatcc agcacccaga tctgatcctg ctgcagtacg
    13801 tggacgatct gctgctggcc gccaccagcg agctggattg ccagcaggga acacgcgccc
    13861 tgctgcagac cctgggaaac ctgggatata gggcatccgc caagaaggcc cagatctgtc
    13921 agaagcaggt gaagtacctg ggctatctgc tgaaggaggg ccagagatgg ctgacagagg
    13981 ccaggaagga gacagtgatg ggccagccaa cacccaagac cccaagacag ctgagggagt
    14041 tcctgggcaa agcaggattt tgcaggctgt tcatcccagg attcgcagag atggcagcac
    14101 ctctgtaccc actgaccaag ccgggcaccc tgtttaattg gggccctgac cagcagaagg
    14161 cctatcagga gatcaagcag gccctgctga cagcaccagc cctgggcctg ccagacctga
    14221 ccaagccttt cgagctgttt gtggatgaga agcagggcta cgccaagggc gtgctgaccc
    14281 agaagctggg accatggaga cggcccgtgg cctatctgtc caagaagctg gacccagtgg
    14341 cagcaggatg gccaccatgc ctgaggatgg tggcagcaat cgccgtgctg acaaaggatg
    14401 ccggcaagct gaccatggga cagccactgg tcatcctggc accacacgca gtggaggccc
    14461 tggtgaagca gcctccagat cgctggctgt ctaacgcccg gatgacacac taccaggccc
    14521 tgctgctgga caccgatcgc gtgcagtttg gccctgtggt ggccctgaat ccagccaccc
    14581 tgctgcctct gccagaggag ggcctgcagc acaactgtct ggacatcctg gcagaggcac
    14641 acggaacaag gccagacctg accgatcagc ccctgcctga cgccgatcac acatggtata
    14701 ccgatggaag ctccctgctg caggagggcc agaggaaggc aggagcagca gtgaccacag
    14761 agacagaagt gatctgggcc aaggccctgc cagcaggcac atccgcccag cgggccgagc
    14821 tgatcgccct gacccaggcc ctgaagatgg ccgagggcaa gaagctgaac gtgtacacag
    14881 actccagata tgccttcgcc accgcacaca tccacggaga gatctacagg cgccggggct
    14941 ggctgacctc tgagggcaag gagatcaaga acaaggatga gatcctggcc ctgctgaagg
    15001 ccctgtttct gcccaagcgg ctgagcatca tccactgtcc tggacaccag aagggacact
    15061 ccgccgaggc aaggggcaat cggatggccg accaggccgc cagaaaggct gctattactg
    15121 aaactcccga cacttccact ctgctgattg aaaactcctc cccttctggc ggctcaaaaa
    15181 gaaccgccga cggcagcgaa ttcgagtctc ccaagaagaa gaggaaagtc ggctctggcc
    15241 ctgccgctaa gagagtgaag ctggactcta gataagctag cctcgagacg cgtactagtg
    15301 ctctagccca attccgcccc ctcccccccc ctaacgttac tggccgaagc cgcttggaat
    15361 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
    15421 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
    15481 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
    15541 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
    15601 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
    15661 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
    15721 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
    15781 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc
    15841 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatttcgaa atggccaagt
    15901 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
    15961 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
    16021 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
    16081 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
    16141 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
    16201 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gacgagcagg
    16261 actgaacgcg ttgaattccc gataatcaac ctctggatta caaaatttgt gaaagattga
    16321 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
    16381 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
    16441 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
    16501 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
    16561 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
    16621 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
    16681 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
    16741 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
    16801 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
    16861 ccgcctcccc gcatcgggaa ttctgtcgac tggtaccttt aagaccaatg acttacaagg
    16921 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
    16981 cccaacgaag acaaaatcgt cgagagatgc tgcatataag cagctgcttt ttgcttgtac
    17041 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    17101 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    17161 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    17221 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
    17281 agagagtgag aggccttgac attataatag atttagcagg aattgaacta ggagtggagc
    17341 acacaggcaa agctgcagaa gtacttggaa gaagccacca gagatactca cgattctgca
    17401 catacctggc taatcccaga tcctaaggat tacattaagt ttactaacat ttatataatg
    17461 atttatagtt taaagtataa acttatctaa tttactattc tgacagatat taattaatcc
    17521 tcaaatatca taagagatga ttactattat ccccattta
//
