LOCUS       sequence-408415-       13629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer plasmid containing CMV-driven expression 
            cassette encoding MLH1dn for prime editing (PE4 and PE5 systems)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13629)
  AUTHORS   Bulcaen M, Kortleven P, Liu RB, Maule G, Dreano E, Kelly M, Ensinck 
            MM, Thierie S, Smits M, Ciciani M, Hatton A, Chevalier B, Ramalho 
            AS, Casadevall I Solvas X, Debyser Z, Vermeulen F, Gijsbers R, 
            Sermet-Gaudelus I, Cereseto A, Carlon MS
  TITLE     Prime editing functionally corrects cystic fibrosis-causing CFTR 
            mutations in human organoids and airway epithelial cells.
  JOURNAL   Cell Rep Med. 2024 May 21;5(5):101544. doi: 
            10.1016/j.xcrm.2024.101544. Epub 2024 May 1.
  PUBMED    38697102
REFERENCE   2  (bases 1 to 13629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Med."; date: "2024-05-21"; volume: "5(5):101544. doi"; pages: " 
            10.1016/j.xcrm.2024.101544. Epub 2024 May 1"
COMMENT     Sequence Label: Lenti-CMV-MLH1dn-IRES-puroR
FEATURES             Location/Qualifiers
     source          1..13629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1191..1210)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1424..1443)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1543..1565
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1603..1621)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1689..1793
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1794..1862,1863..2654)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2012..2031)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2825..3413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3314..3333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3567..3584
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3654..3674)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3659..3988
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3839..3974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5122..5187
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5317..5337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5762..5896
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5799..5818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5853..5872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6064..6697
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6744..6869
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7362..7595
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7780..7824
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8117..8234
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8293..8596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8597..8800
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8751..8771
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8797..8821
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    11180..11732
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(11324..11341)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     11551..11570
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             11732..12334
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     12231..12251
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12354..12942
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12407..12427)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12825..12836)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             13112..13292
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 acacaagagg aaactgagag ggaaagatgt tgaagtaatt ttcccacaat tacagcatcc
       61 gttagttacg actctatgat cttctgacac aaattccatt tactcctcac cctatgactc
      121 agtcgaatat atcaaagtta tggacattat gctaagtaac aaattaccct tttatatagt
      181 aaatactgag tagattgaga gaagaaattg tttgcaaacc tgaatagctt caagaagaag
      241 agaagtgagg ataagaataa cagttgtcat ttaacaagtt ttaacaagta acttggttag
      301 aaagggattc aaatgcataa agcaagggat aaatttttct ggcaacaaga ctatacaata
      361 taaccttaaa tatgacttca aataattgtt ggaacttgat aaaactaatt aaatattatt
      421 gaagattatc aatattataa atgtaattta cttttaaaaa gggaacatag aaatgtgtat
      481 cattagagta gaaaacaatc cttattatca caatttgtca aaacaagttt gttattaaca
      541 caagtagaat actgcattca attaagttga ctgcagattt tgtgttttgt taaaattaga
      601 aagagataac aacaatttga attattgaaa gtaacatgta aatagttcta catacgttct
      661 tttgacatct tgttcaatca ttgatcgaag ttctttatct tggaagaatt tgttccaaag
      721 actctgaaat aaggaaaaca atctattata tagtctcaca cctttgtttt acttttagtg
      781 atttcaattt aataatgtaa atggttaaaa tttattcttc tctgagatca tttcacattg
      841 cagatagaaa acctgagact ggggtaattt ttattaaaat ctaatttaat ctcagaaaca
      901 catctttatt ctaacatcaa tttttccagt ttgatattat catataaagt cagccttcct
      961 catctgcagg ttccacaaca aaaatccaac caactgtgga tcaaaaatat tgggaaaaaa
     1021 ttaaaaatag caatacaaca ataaaaaaat acaaatcaga aaaacagcac agtataacaa
     1081 ctttatttag catttacaat ctattaggta ttataagtaa tctagccaga tcctctacgc
     1141 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     1201 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     1261 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     1321 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     1381 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     1441 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     1501 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1561 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     1621 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1681 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1741 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1801 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1861 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1921 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1981 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2041 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2101 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2161 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2221 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2281 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2341 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2401 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2461 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2521 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2581 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2641 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2701 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2761 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2821 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2881 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2941 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3001 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3061 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3121 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3181 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3241 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3301 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3361 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3421 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3481 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3541 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3601 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     3661 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3721 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3781 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3841 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3901 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3961 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     4021 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     4081 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     4141 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     4201 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     4261 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     4321 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     4381 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     4441 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     4501 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     4561 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     4621 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     4681 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     4741 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     4801 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     4861 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     4921 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     4981 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     5041 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     5101 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     5161 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     5221 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     5281 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     5341 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     5401 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     5461 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     5521 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     5581 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     5641 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     5701 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     5761 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5881 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     5941 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     6001 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     6061 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     6121 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     6181 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     6241 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     6301 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     6361 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     6421 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     6481 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     6541 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6601 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6661 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     6721 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     6781 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     6841 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     6901 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     6961 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     7021 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     7081 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     7141 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     7201 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     7261 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     7321 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     7381 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     7441 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     7501 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     7561 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     7621 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     7681 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     7741 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     7801 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     7861 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     7921 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     7981 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     8041 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat tcacaaatgg
     8101 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     8161 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     8221 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ggcgataagc
     8281 ttgggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     8341 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     8401 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     8461 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     8521 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     8581 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     8641 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     8701 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     8761 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
     8821 cgcctggaga cgccatccac gctgttttga cctccataga agacaccgac tctagaatga
     8881 gcttcgttgc tggagtcatc cggagactgg acgagacagt ggtgaacaga attgccgccg
     8941 gcgaggtgat ccagagacct gccaatgcaa taaaggagat gatcgagaac tgtctggacg
     9001 ccaagtccac aagcattcag gtgatcgtga aggagggcgg actgaagctg atccagatcc
     9061 aagacaacgg cacaggcatc agaaaggaag atctggacat cgtgtgtgaa cggttcacca
     9121 catctaagct gcagtctttt gaggatctgg cctctatcag tacctacggc ttcagaggcg
     9181 aggccctggc cagcatcagc cacgtggccc atgtgaccat caccaccaaa accgccgacg
     9241 gcaaatgcgc ttatcgcgct agctacagcg acggcaagct gaaagccccg ccaaagcctt
     9301 gcgccggcaa ccagggtaca cagataacag tggaggatct gttctacaac atcgccaccc
     9361 ggagaaaggc cctgaaaaat cccagcgagg agtacggcaa gatcctggaa gtcgtcggca
     9421 gatactccgt gcacaacgcc ggaatcagct ttagcgtaaa gaagcaggga gaaaccgtgg
     9481 ccgatgtgcg caccctgcca aatgccagca ccgtggataa catcagaagc attttcggaa
     9541 atgccgtgtc cagagaactg atcgagatcg gctgcgaaga taagaccctg gcttttaaga
     9601 tgaacggcta catcagcaac gccaattact ctgtgaagaa gtgcatcttt cttctgttca
     9661 tcaaccacag actggtggaa agcaccagcc tgcggaaagc catcgagaca gtgtacgccg
     9721 cctacctgcc taagaacacc caccccttcc tgtacctgag cctcgagatc agccctcaga
     9781 acgtggacgt caatgtgcat cctacaaagc acgaggtgca cttcctgcac gaggaaagca
     9841 tcctggaaag agtgcagcag cacattgaga gcaagctgct gggctctaac agcagcagaa
     9901 tgtacttcac acagaccctg ctgcctggcc tggccggccc ctcaggcgaa atggttaagt
     9961 ccacaacctc tctgacctca tccagcacca gcggttcttc cgataaggtg tacgcccacc
    10021 agatggtgcg gaccgactct cgggagcaga agctggacgc ctttctgcaa cctctgagca
    10081 aacctctgag ctctcagcct caggccatcg tgaccgagga caagacagat atctcctccg
    10141 gccgtgccag acagcaggac gaagaaatgc tcgagctgcc agctcctgcc gaggtggccg
    10201 ccaagaacca gagcctggag ggagatacca caaagggcac cagcgaaatg agcgagaagc
    10261 ggggccctac ctccagcaac cccagaaaac ggcaccggga ggacagcgac gtggaaatgg
    10321 tggaggacga cagcagaaag gaaatgacag ccgcttgtac ccctagaaga agaatcatca
    10381 acctgacctc cgtgctgagc ctgcaggagg agatcaacga gcagggccac gaggtgctga
    10441 gagagatgct gcacaatcac agcttcgtgg gctgcgtgaa ccctcaatgg gccctggctc
    10501 agcatcaaac aaagctgtac ctgctgaaca ccaccaagct gagcgaagag ctgttctacc
    10561 agatcctcat ctacgacttc gccaacttcg gcgtgctacg cctgagcgag cccgcccctc
    10621 tgtttgacct ggccatgctg gctctggata gcccagaaag cggctggaca gaagaggacg
    10681 gacctaaaga ggggctggct gaatacatcg tggagttcct gaagaaaaag gccgagatgc
    10741 tggccgacta cttttctctg gaaatcgacg aggaaggcaa cctgatcggc ctgcctctgc
    10801 tgatcgataa ctacgtgcct cccctggaag gcctgcccat cttcatcctg agactggcta
    10861 cagaggtgaa ctgggacgag gaaaaggaat gcttcgagtc tctgagcaag gagtgcgcca
    10921 tgttctatag catcagaaaa cagtacatct ctgaagagag cactctgtct ggccagcaga
    10981 gtgaagtgcc cggaagcatc cccaacagct ggaagtggac cgtggaacac atcgtgtaca
    11041 aggccctgcg gagccacatt ctccctccta agcacttcac cgaggacggc aacatcctgc
    11101 agctggccaa cctgcccgac ctttataagg ttttctaaac gcgtgctcta gcccaattcc
    11161 gccccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
    11221 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
    11281 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
    11341 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
    11401 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
    11461 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
    11521 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
    11581 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
    11641 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
    11701 gtggttttcc tttgaaaaac acgatgataa tatgggcacc gagtacaagc ccacggtgcg
    11761 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga
    11821 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct
    11881 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
    11941 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
    12001 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
    12061 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
    12121 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
    12181 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
    12241 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
    12301 cacctggtgc atgacccgca agcccggtgc ctgaggcgcg ttgaattccc gataatcaac
    12361 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
    12421 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
    12481 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
    12541 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
    12601 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
    12661 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
    12721 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
    12781 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
    12841 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
    12901 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgggaa ttctgtcgac
    12961 tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
    13021 aaaagggggg actggaaggg ctaattcact cccaacgaag acaaaatcgt cgagagatgc
    13081 tgcatataag cagctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
    13141 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    13201 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    13261 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
    13321 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac attataatag
    13381 atttagcagg aattgaacta ggagtggagc acacaggcaa agctgcagaa gtacttggaa
    13441 gaagccacca gagatactca cgattctgca catacctggc taatcccaga tcctaaggat
    13501 tacattaagt ttactaacat ttatataatg atttatagtt taaagtataa acttatctaa
    13561 tttactattc tgacagatat taattaatcc tcaaatatca taagagatga ttactattat
    13621 ccccattta
//
