LOCUS       sequence-408421-        9528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express EPN24-PCP-IRES-eGFP and blasticidin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Horns F, Martinez JA, Fan C, Haque M, Linton JM, Tobin V, Santat L, 
            Maggiolo AO, Bjorkman PJ, Lois C, Elowitz MB
  TITLE     Engineering RNA export for measurement and manipulation of living 
            cells.
  JOURNAL   Cell. 2023 Jul 10:S0092-8674(23)00689-X. doi: 
            10.1016/j.cell.2023.06.013.
  PUBMED    37437570
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Jul 10:S0092-8674(23)00689-X. doi: 10.1016/j.cell.2023.06.013."
COMMENT     Sequence Label: pFH2.217_PB-EPN24-PCP-IRES-eGFP-PGK-Blast
FEATURES             Location/Qualifiers
     source          1..9528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   14..48
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        612..860
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     612..632
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     783..802
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        942..1153
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1166..1434
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2632..2661
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    3040..3613
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3207..3224)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3434..3453
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3622..3624,3625..4338)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             3622..4335
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3664..3685)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3925..3944)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4272..4293
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4466..4587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4503..4522)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4557..4576
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4960..4979
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      5057..5066
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5063..5461
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5479..5703
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6130..6192
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6691..6707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6691..6707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6704..6726)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6715..6731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6739..6745,6746..6763,6764..6769))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6784..6805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6922..6939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7093..7681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7173..7192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7852..8646)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7852..8646)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7963..7982)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8573..8592
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8647..8751)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8777..9232)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8914..8935)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9126..9145
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9359..9381
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9373..9390
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9374..9390
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcagctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
       61 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      121 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      181 ataacaaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      241 actcatacga taattatatt gttatttcgt gttctactta cgtgataact tattatatat
      301 atattttctt gttatagata tccttgaggg acagcccccc cccaaagccc ccagggatgt
      361 aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc
      421 ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg
      481 tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg
      541 gggggatacg gggaaaaatc gattaactat aacggtccta aggtagcgaa cacctgcgct
      601 cgagcgacgt cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
      661 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
      721 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      781 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      841 tgtggaaagg acgaaacacc gaccaggtgg gcccacgcgt ttttttcacc tgcattaccc
      901 tgttatccct aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc
      961 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg
     1021 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     1081 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     1141 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc
     1201 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc
     1261 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg
     1321 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct
     1381 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct
     1441 gtgaccggcg cctacgctag cggatccgcc accatgcacg gtctgcagga cgacccggac
     1501 ctgcaagcac tgctcaaggg cagccagctg ctgaaagtta aatcttcttc ttggcgtcgt
     1561 gaacgtttct acaaactcca ggaggactgc aaaaccatct ggcaggaatc ccgtaaagtt
     1621 atgcgttctc cggaatctca gctgttctct atcgaagaca tccaggaagt tcgtatgggt
     1681 caccgtaccg aaggtctgga aaaattcgcg cgtgacatcc cggaagaccg ttgcttctcc
     1741 atcgttttca aagaccagcg taacaccctg gacctgatcg cgccgtctcc ggcggacgcg
     1801 cagcactggg ttcagggtct gcgtaaaatc atccaccact ctggttctat ggaccaacgt
     1861 cagaaactgc agtctcgtcc ggaaccgacc gcaccgccgg aagaatcttt ccgttctggt
     1921 gttgaaacca ccacgccacc gcaaaaacag gagccgatcg acaaagaact gtacccgctg
     1981 acctctctgc gttctctgtt cggtaacgac ccgtcttctc agaagatcga agagctgttc
     2041 aagaaacaca agatcgttgc cgtgctgcgt gccaatagtg tggaagaagc gaaaaagaaa
     2101 gcgctggcgg ttttcctggg cggcgttcat ctgattgaaa ttacctttac cgtgccggat
     2161 gcggataccg tgattaagga actgagcttt ctgaaggaaa tgggcgcgat tattggtgcg
     2221 ggcaccgtga ccagcgtgga gcagtgccgt aaagcggtgg aaagtggcgc cgagttcatt
     2281 gtgagtccgc acctggacga ggaaattagc caattttgca aggagaaggg tgtgttctat
     2341 atgccaggcg ttatgacccc gaccgaactg gtgaaagcca tgaaactggg ccataccatc
     2401 ttaaaactgt ttccgggtga ggtggtgggt ccgcagtttg ttaaagcgat gaaaggtccg
     2461 tttccgaatg tgaaatttgt gccaaccggc ggtgttaatc tggacaatgt gtgcgaatgg
     2521 ttcaaagcgg gcgtgctggc cgtgggcgtg ggcagcgcgt tagtgaaagg caccccggtg
     2581 gaagtggcgg aaaaggccaa ggcgttcgtt gagaagattc gtggctgcac cgaacagaag
     2641 ctgatctccg aagaagacct gatgggttcc aaaaccatcg ttcttagcgt cggcgaggct
     2701 actcgcactc tgactgagat ccagtccacc gcagacagac agatcttcga agagaaggtc
     2761 gggcctctgg tgggtcggct gcgcctcacg gccagcctca gacaaaacgg agccaagacc
     2821 gcgtatcgcg tcaacctgaa actggatcag gcggacgtcg ttgatagcgg acttccgaaa
     2881 gtgcgctaca ctcaggtatg gtcccacgac gtgacaatcg ttgcgaatag caccgaggcc
     2941 agccgcaaat ctttgtacga tttgaccaag tccctcgtcg cgaccagcca ggtcgaagat
     3001 cttgtcgtca accttgtgcc gctgggccgt taagaattcc ccctctccct cccccccccc
     3061 taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt
     3121 ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt
     3181 gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt
     3241 cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct
     3301 ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt
     3361 ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt
     3421 ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa
     3481 ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
     3541 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
     3601 aacacgatga taacctgcag gatgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     3661 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     3721 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     3781 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     3841 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3901 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     3961 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4021 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     4081 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     4141 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     4201 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     4261 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tcacggcatg
     4321 gacgagctgt acaagtaagc ggccgcgtcg acgactctag atcataatca gccataccac
     4381 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     4441 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     4501 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4561 tttgtccaaa ctcatcaatg tatcttaagc gccggcgcta cacaagtggc ctctggcctc
     4621 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     4681 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     4741 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     4801 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     4861 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     4921 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     4981 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa
     5041 gcttaccatg ggtaccgcca ccatggccaa gcctttgtct caagaagaat ccaccctcat
     5101 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagattaca gcgtcgccag
     5161 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
     5221 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
     5281 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
     5341 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
     5401 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
     5461 aggtcacccg cgcgcggact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     5521 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     5581 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     5641 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     5701 tggttcgaac cagtacgatg gttttccccg tatcccccca ggtgtctgca ggctcaaaga
     5761 gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc ccgggctgtc
     5821 cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg agccccgggc
     5881 ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg gctttggggg
     5941 ggggctgtcc ctgataaaag ttttgttact ttatagaaga aattttgagt ttttgttttt
     6001 ttttaataaa taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt
     6061 gtaaatataa taaaacttaa tatctattca aattaataaa taaacctcga tatacagacc
     6121 gataaaacac atgcgtcaat tttacgcatg attatcttta acgtacgtca caatatgatt
     6181 atctttctag ggttaatcta gctgcggcgc gccatcagga tcatatcgtc gggtcttttt
     6241 tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg
     6301 ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca
     6361 ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg
     6421 cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc
     6481 ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca
     6541 gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg
     6601 tcagcgagga cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt
     6661 tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6721 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6781 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6841 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6901 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     7561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gtcagaagaa
     7861 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
     7921 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
     7981 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
     8041 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
     8101 ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
     8161 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
     8221 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
     8281 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag
     8341 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
     8401 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
     8461 gtcttgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
     8521 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
     8581 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
     8641 aatcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8701 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8761 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     8821 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     8881 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     8941 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     9001 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     9061 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     9121 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     9181 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     9241 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     9301 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     9361 cagtcacgac gttgtaaaac gacggccagt gagcgcgcct cgttcattca cgtttttgaa
     9421 cccgtggagg acgggcagac tcgcggtgca aatgtgtttt acagcgtgat ggagcagatg
     9481 aagatgctcg acacgctgca gaacacgagc tccaccgcgg ggcgcgcc
//
