LOCUS       sequence-408424-        7842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express human STEAP3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Horns F, Martinez JA, Fan C, Haque M, Linton JM, Tobin V, Santat L, 
            Maggiolo AO, Bjorkman PJ, Lois C, Elowitz MB
  TITLE     Engineering RNA export for measurement and manipulation of living 
            cells.
  JOURNAL   Cell. 2023 Jul 10:S0092-8674(23)00689-X. doi: 
            10.1016/j.cell.2023.06.013.
  PUBMED    37437570
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2023 
            Jul 10:S0092-8674(23)00689-X. doi: 10.1016/j.cell.2023.06.013."
COMMENT     Sequence Label: pJAM1.17_PB-STEAP3-PGK-Blast
FEATURES             Location/Qualifiers
     source          1..7842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   14..48
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        660..871
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             884..1152
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     polyA_signal    2780..2901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2817..2836)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2871..2890
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3274..3293
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3371..3380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3377..3775
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    3793..4017
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4444..4506
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5005..5021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5005..5021)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5018..5040)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5029..5045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5053..5059,5060..5077,5078..5083))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5098..5119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5236..5253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5407..5995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5487..5506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6166..6960)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6166..6960)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6277..6296)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6887..6906
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6961..7065)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7091..7546)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7228..7249)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7440..7459
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7673..7695
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7687..7704
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7688..7704
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcagctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
       61 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      121 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      181 ataacaaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      241 actcatacga taattatatt gttatttcgt gttctactta cgtgataact tattatatat
      301 atattttctt gttatagata tccttgaggg acagcccccc cccaaagccc ccagggatgt
      361 aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc
      421 ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg
      481 tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg
      541 gggggatacg gggaaaaatc gattaactat aacggtccta aggtagcgaa cacctgcgct
      601 cgagcgacgt cattaccctg ttatccctag gatctgcgat cgctccggtg cccgtcagtg
      661 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      721 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      781 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      841 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
      901 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
      961 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     1021 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     1081 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     1141 ccgttacaga tccaagctgt gaccggcgcc tacgctagcg ccaccatgcc agaagagatg
     1201 gacaagccac tgatcagcct ccacctggtg gacagcgata gtagccttgc caaggtcccc
     1261 gatgaggccc ccaaagtggg catcctgggt agcggggact ttgcccgctc cctggccaca
     1321 cgcctggtgg gctctggctt caaagtggtg gtggggagcc gcaaccccaa acgcacagcc
     1381 aggctgtttc cctcagcggc ccaagtgact ttccaagagg aggcagtgag ctccccggag
     1441 gtcatctttg tggctgtgtt ccgggagcac tactcttcac tgtgcagtct cagtgaccag
     1501 ctggcgggca agatcctggt ggatgtgagc aaccctacag agcaagagca ccttcagcat
     1561 cgtgagtcca atgctgagta cctggcctcc ctcttcccca cttgcacagt ggtcaaggcc
     1621 ttcaatgtca tctctgcctg gaccctgcag gctggcccaa gggatggtaa caggcaggtg
     1681 cccatctgcg gtgaccagcc agaagccaag cgtgctgtct cggagatggc gctcgccatg
     1741 ggcttcatgc ccgtggacat gggatccctg gcgtcagcct gggaggtgga ggccatgccc
     1801 ctgcgcctcc tcccggcctg gaaggtgccc accctgctgg ccctggggct cttcgtctgc
     1861 ttctatgcct acaacttcgt ccgggacgtt ctgcagccct atgtgcagga aagccagaac
     1921 aagttcttca agctgcccgt gtccgtggtc aacaccacac tgccgtgcgt ggcctacgtg
     1981 ctgctgtcac tcgtgtactt gcccggcgtg ctggcggctg ccctgcagct gcggcgcggc
     2041 accaagtacc agcgcttccc cgactggctg gaccactggc tacagcaccg caagcagatc
     2101 gggctgctca gcttcttctg cgccgccctg cacgccctct acagcttctg cttgccgctg
     2161 cgccgcgccc accgctacga cctggtcaac ctggcagtca agcaggtctt ggccaacaag
     2221 agccacctct gggtggagga ggaggtctgg cggatggaga tctacctctc cctgggagtg
     2281 ctggccctcg gcacgttgtc cctgctggcc gtgacctcac tgccgtccat tgcaaactcg
     2341 ctcaactgga gggagttcag cttcgttcag tcctcactgg gctttgtggc cctcgtgctg
     2401 agcacactgc acacgctcac ctacggctgg acccgcgcct tcgaggagag ccgctacaag
     2461 ttctacctgc ctcccacctt cacgctcacg ctgctggtgc cctgcgtcgt catcctggcc
     2521 aaagccctgt ttctcctgcc ctgcatcagc cgcagactcg ccaggatccg gagaggctgg
     2581 gagagggaga gcaccatcaa gttcacgctg cccacaggcc acgccctggc cgagaagacg
     2641 agccacgtat aggcggccgc gtcgacgact ctagatcata atcagccata ccacatttgt
     2701 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     2761 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     2821 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2881 caaactcatc aatgtatctt aagcgccggc gctacacaag tggcctctgg cctcgcacac
     2941 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     3001 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     3061 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     3121 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     3181 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     3241 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     3301 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     3361 catgggtacc gccaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     3421 agcaacggct acaatcaaca gcatccccat ctctgaagat tacagcgtcg ccagcgcagc
     3481 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     3541 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     3601 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     3661 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     3721 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaaggtca
     3781 cccgcgcgcg gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     3841 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     3901 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     3961 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggttc
     4021 gaaccagtac gatggttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg
     4081 agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca
     4141 cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg
     4201 ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg ggggggggct
     4261 gtccctgata aaagttttgt tactttatag aagaaatttt gagtttttgt ttttttttaa
     4321 taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt aagtgtaaat
     4381 ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca gaccgataaa
     4441 acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat gattatcttt
     4501 ctagggttaa tctagctgcg gcgcgccatc aggatcatat cgtcgggtct tttttccggc
     4561 tcagtcatcg cccaagctgg cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt
     4621 cccgcgaggt tgaagcggca tggaaagagt ttgccgagga tgactgctgc tgcattgacg
     4681 ttgagcgaaa acgcacgttt accatgatga ttcgggaagg tgtggccatg cacgccttta
     4741 acggtgaact gttcgttcag gccacctggg ataccagttc gtcgcggctt ttccggacac
     4801 agttccggat ggtcagcccg aagcgcatca gcaacccgaa caataccggc gacagccgga
     4861 actgccgtgc cggtgtgcag attaatgaca gcggtgcggc gctgggatat tacgtcagcg
     4921 aggacgggta tcctggctgg atgccgcaga aatggacatg gataccccgt gagttacccg
     4981 gcgggcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5041 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5101 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5161 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5221 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5281 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5341 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5401 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5461 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5521 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5581 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5641 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5701 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5761 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5821 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5881 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5941 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6001 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6061 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6121 ttttaaatca atctaaagta tatatgagta aacttggtct gacagtcaga agaactcgtc
     6181 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     6241 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     6301 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     6361 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc
     6421 gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     6481 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     6541 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     6601 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     6661 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     6721 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg
     6781 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     6841 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     6901 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     6961 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7021 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7081 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     7141 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     7201 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     7261 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     7321 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     7381 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     7441 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     7501 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     7561 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     7621 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     7681 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
     7741 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
     7801 ctcgacacgc tgcagaacac gagctccacc gcggggcgcg cc
//
