LOCUS       sequence-408427-       13472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expressing doxycyclin-inducible cDNA of wild 
            type PTEN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13472)
  AUTHORS   Loh ZN, Wang ME, Wan C, Asara JM, Ji Z, Chen M
  TITLE     Nuclear PTEN Regulates Thymidylate Biosynthesis in Human Prostate 
            Cancer Cell Lines.
  JOURNAL   Metabolites. 2023 Aug 11;13(8):939. doi: 10.3390/metabo13080939.
  PUBMED    37623882
REFERENCE   2  (bases 1 to 13472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Metabolites."; date: "2023-08-11"; volume: "13(8):939. doi"; pages:
            " 10.3390/metabo13080939"
COMMENT     Sequence Label: pTRIPZ-Wt-PTEN
FEATURES             Location/Qualifiers
     source          1..13472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        4222..5433
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5270..5289
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5446..5479)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             5486..6193
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(6194..6227)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    6250..6822
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6417..6434)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6644..6663
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6825..7427
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7324..7344
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7443..8031
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7496..7516)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7914..7925)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8238..8471
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8572..8589)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8578..8802
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8848..9276
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8935..8954)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9145..9166
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9285..9305)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9290..9619
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9470..9605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9532..9551
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    10827..10948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10997..11013)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10997..11013)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11010..11032)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11021..11037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11045..11051,11052..11069,11070..11075))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11090..11111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11228..11245)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11399..11987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11479..11498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12158..12949,12950..13018))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12781..12800
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13019..13123)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13171..13305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtgcc
     3001 accatgacag ccatcatcaa agagatcgtt agcagaaaca aaaggagata tcaagaggat
     3061 ggattcgact tagacttgac ctatatttat ccaaacatta ttgctatggg atttcctgca
     3121 gaaagacttg aaggcgtata caggaacaat attgatgatg tagtaaggtt tttggattca
     3181 aagcataaaa accattacaa gatatacaat ctttgtgctg aaagacatta tgacaccgcc
     3241 aaatttaatt gcagagttgc acaatatcct tttgaagacc ataacccacc acagctagaa
     3301 cttatcaaac ccttttgtga agatcttgac caatggctaa gtgaagatga caatcatgtt
     3361 gcagcaattc actgtaaagc tggaaaggga cgaactggtg taatgatatg tgcatattta
     3421 ttacatcggg gcaaattttt aaaggcacaa gaggccctag atttctatgg ggaagtaagg
     3481 accagagaca aaaagggagt aactattccc agtcagaggc gctatgtgta ttattatagc
     3541 tacctgttaa agaatcatct ggattataga ccagtggcac tgttgtttca caagatgatg
     3601 tttgaaacta ttccaatgtt cagtggcgga acttgcaatc ctcagtttgt ggtctgccag
     3661 ctaaaggtga agatatattc ctccaattca ggacccacac gacgggaaga caagttcatg
     3721 tactttgagt tccctcagcc gttacctgtg tgtggtgata tcaaagtaga gttcttccac
     3781 aaacagaaca agatgctaaa aaaggacaaa atgtttcact tttgggtaaa tacattcttc
     3841 ataccaggac cagaggaaac ctcagaaaaa gtagaaaatg gaagtctatg tgatcaagaa
     3901 atcgatagca tttgcagtat agagcgtgca gataatgaca aggaatatct agtacttact
     3961 ttaacaaaaa atgatcttga caaagcaaat aaagacaaag ccaaccgata cttttctcca
     4021 aattttaagg tgaagctgta cttcacaaaa acagtagagg agccgtcaaa tccagaggct
     4081 agcagttcaa cttctgtaac accagatgtt agtgacaatg aacctgatca ttatagatat
     4141 tctgacacca ctgactctga tccagagaat gaaccttttg atgaagatca gcatacacaa
     4201 attacaaaag tctgaacgcg tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc
     4261 ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt
     4321 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     4381 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     4441 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     4501 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     4561 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     4621 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
     4681 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
     4741 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga
     4801 cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
     4861 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
     4921 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc
     4981 gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg
     5041 agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
     5101 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
     5161 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
     5221 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
     5281 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
     5341 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct
     5401 aaattctggc cgtttttggc ttttttgtta gacgctagcg gatccataac ttcgtatagt
     5461 atacattata cgaagttatg ccaccatgtc taggctggac aagagcaaag tcataaacgg
     5521 agctctggaa ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca
     5581 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     5641 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     5701 cgagtcatgg caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc
     5761 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     5821 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     5881 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     5941 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     6001 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
     6061 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct
     6121 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgattttg accttgacat
     6181 gctccccggg taaataactt cgtatagtat acattatacg aagttatgga tccgcggccg
     6241 caaattccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     6301 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     6361 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     6421 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     6481 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     6541 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     6601 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     6661 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     6721 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     6781 gaaccacggg gacgtggttt tcctttgaaa aacacgataa taccatggcc accgagtaca
     6841 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
     6901 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
     6961 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     7021 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     7081 gggcggtgtt cgccgagatc ggctcgcgca tggccgagtt gagcggttcc cggctggccg
     7141 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     7201 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     7261 tccccggagt ggaggcggcc gagcgcgctg gggtgcccgc cttcctggag acctccgcgc
     7321 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     7381 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagtt cgcgtctgga
     7441 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     7501 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     7561 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     7621 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     7681 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     7741 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     7801 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     7861 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7921 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7981 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta
     8041 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     8101 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     8161 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     8221 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     8281 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8341 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8401 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8461 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     8521 atgaatatca gagagtgaga ggccttgaca ttgtttaaac ccgctgatca gcctcgactg
     8581 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     8641 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     8701 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     8761 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     8821 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     8881 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8941 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     9001 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     9061 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     9121 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     9181 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     9241 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     9301 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9361 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9421 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     9481 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9541 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     9601 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     9661 gcacgtgatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     9721 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     9781 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     9841 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     9901 tgacattggg gaattaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     9961 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
    10021 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
    10081 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
    10141 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
    10201 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
    10261 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
    10321 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
    10381 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
    10441 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
    10501 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
    10561 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
    10621 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
    10681 cgagggcaaa ggaatagcac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
    10741 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
    10801 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
    10861 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    10921 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
    10981 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    11041 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    11101 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    11161 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    11221 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    11281 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    11341 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    11401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    11461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    11521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    11581 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    11641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    11701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    11761 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    11821 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    11881 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    11941 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    12001 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    12061 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    12121 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    12181 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    12241 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    12301 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    12361 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    12421 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    12481 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    12541 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    12601 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    12661 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    12721 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    12781 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    12841 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    12901 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    12961 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    13021 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    13081 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    13141 tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac
    13201 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    13261 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    13321 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    13381 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    13441 atttgttaaa tatgtactac aaacttagta gt
//
