LOCUS       sequence-408431-       10292 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid containing DDX3X reporter HART for ODC1 5UTR - 
            PuroR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Wilkins KC, Schroeder T, Gu S, Revalde JL, Floor SN
  TITLE     A novel reporter for helicase activity in translation uncovers DDX3X
            interactions.
  JOURNAL   RNA. 2024 Jul 16;30(8):1041-1057. doi: 10.1261/rna.079837.123.
  PUBMED    38697667
REFERENCE   2  (bases 1 to 10292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.079837"; journalName: "RNA"; date: "2024-07-16- 16"; 
            volume: "30"; issue: "8"; pages: "1041-1057"
COMMENT     Sequence Label: KW78_HART_ODC1_PuroR
FEATURES             Location/Qualifiers
     source          1..10292
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        1131..1510
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1511..1714
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1729..1909
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1956..2081
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2574..2807
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2992..3036
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3303..3420
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    3557..3691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3911..4510)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3994..4014)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4491..4510
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(4523..4576)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(4595..5302,5303..5305,5306..5308))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             complement(join(4595..5302,5303..5305,5306..5308))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(4634..4655)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4983..5002
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5242..5263
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5305..5314
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5332..5370)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6482..6500)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6750..6769
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7193..7781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7246..7266)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7664..7675)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7868..8101
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8173..8294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      8334..8469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8489..8508)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8490..8508)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8518..8535)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8518..8534)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8527..8549)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8676..9131
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8763..8782)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8973..8994
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9158..9261
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9262..9330,9331..10122)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9480..9499)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcggactgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttggcca ttgcatacgt tgtatccata
     1081 tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt gacattgatt
     1141 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1201 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1261 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1321 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1381 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1441 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1501 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     1561 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     1621 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     1681 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccggg gtctctctgg
     1741 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     1801 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     1861 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     1921 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1981 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     2041 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     2101 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     2161 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     2221 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     2281 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     2341 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     2401 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     2461 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagta
     2521 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     2581 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     2641 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     2701 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     2761 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     2821 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     2881 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     2941 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     3001 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     3061 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtatg tttaagaata
     3121 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     3181 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     3241 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgctta
     3301 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     3361 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     3421 atcgatcacg agactagcct cgagagatct gatcataatc agccatacca catttgtaga
     3481 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     3541 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaggcaatag
     3601 catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg gtttgtccaa
     3661 actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg cgcctgtctt
     3721 aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca gtagctctga
     3781 cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat tggctgtcca
     3841 gcttagctcg caggggaggt ggtctggatc caccggtccg gtttaattaa cgtagcacgc
     3901 gtttagtcat ctaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt ccttcgggca
     3961 cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg ttgcggggcg
     4021 cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact ccggggagca
     4081 cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg gtggccagga
     4141 accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt tgctgcgcgg
     4201 ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac accgcccccg
     4261 cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc gcgacccaca
     4321 ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg gtgacccgct
     4381 cgatgtggcg gtccgggtcg acggtgtggc gcgtggcggg gtagtcggcg aacgcggcgg
     4441 cgagggtgcg tacggcccgg gggacgtcgt cgcgggtggc gaggcgcacc gtgggcttgt
     4501 actcggtcat ggtggcgacc ggtgggccag gattctcctc gacgtcaccg catgttagca
     4561 gacttcctct gccctctccc gatccgctag cagtgtacag ctcgtccatg ccgagagtga
     4621 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     4681 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     4741 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     4801 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     4861 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     4921 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     4981 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     5041 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     5101 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     5161 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     5221 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     5281 tgaacagctc ctcgcccttg ctcaccatgg tggctagcgg ttcactaaac gagctctgct
     5341 tatataggcc tcccaccgta cacgcctacc ctcgagaagc ttgatatcga attccacggg
     5401 gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg
     5461 gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt
     5521 tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc
     5581 tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga
     5641 agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg
     5701 ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg
     5761 ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc
     5821 cgcgcgacgt cggcccgccg gcgccccacc agctccgcgc gggcccgggt tggccaccgc
     5881 cgggcccccg cccctccccc ggcggtgtcc cggccggaac cgatcgtggc tggtttgagc
     5941 tggtgcgtct ccatggcgac ccgccggtgc tataagtagg gagcggcgtg ccgtggggct
     6001 ttgtcagtcc ctcctgtagc cgccgccgcc gccgcccgcc gcccctctgc cagcagctcc
     6061 ggcgccacct cgggccggcg tctccggcgg gcgggagcca ggcgctgacg ggcgcggcgg
     6121 gggcggccga gcgctcctgc ggctgcgact caggctccgg cgtctgcgct tccccatggg
     6181 gctggcctgc ggcgcctggg cgctctgaga ttgtcactgc tgttccaagg gcacacgcag
     6241 agggatttgg aattcctgga gagttgcctt tgtgagaagc tggaaatatt tctttcaatt
     6301 ccatctctta gttttccata ggaacatcaa gaaatcatgg tttcaaaagg tgaagaagat
     6361 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     6421 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     6481 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     6541 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     6601 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     6661 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     6721 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     6781 gaggcctcct ccgagagaat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     6841 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     6901 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     6961 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     7021 ggcggcatgg acgagctgta caagaagctt agccatggct tcccgccgga ggtggaggag
     7081 caggatgatg gcgcgctgcc catgtcttgt gcccaggaga gcgggatgga ccgtcaccct
     7141 gcagcctgtg cttctgctag gatcaatgtg tagagtcgag tctagagtcg acaatcaacc
     7201 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7261 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7321 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7381 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7441 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7501 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7561 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     7621 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7681 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7741 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta
     7801 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7861 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     7921 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7981 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8041 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     8101 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     8161 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8221 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8281 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     8341 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8401 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     8461 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     8521 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     8581 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     8641 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     8701 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     8761 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8821 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     8881 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     8941 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9001 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     9061 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     9121 gcttacaatt taggtggcac ttttcgggga aatgtgtgcg gaacccctat ttgtttattt
     9181 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     9241 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     9301 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     9361 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     9421 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     9481 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     9541 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     9601 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     9661 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     9721 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     9781 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     9841 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     9901 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     9961 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    10021 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    10081 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    10141 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    10201 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    10261 tcagaccccg tagaaaagat caaaggatct tc
//
