LOCUS       sequence-408449-        9460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pnpr-4 TeTx mCherry unc-54 3' UTR C.elegans AVA and other neurons 
            expression of TeTx RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Meng J, Ahamed T, Yu B, Hung W, Ei Mouridi S, Wang Z, Zhang Y, Wen 
            Q, Boulin T, Gao S, Zhen M
  TITLE     A tonically active master neuron modulates mutually exclusive motor 
            states at two timescales.
  JOURNAL   Sci Adv. 2024 Apr 12;10(15):eadk0002. doi: 10.1126/sciadv.adk0002. 
            Epub 2024 Apr 10.
  PUBMED    38598630
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-04-12"; volume: "10(15):eadk0002. doi"; pages: " 
            10.1126/sciadv.adk0002. Epub 2024 Apr 10"
COMMENT     Sequence Label: pJH4506
FEATURES             Location/Qualifiers
     source          1..9460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5404..6114
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5549..5567)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5817..5836
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(7030..7052)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7152..7171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7253..7271)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7339..7443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7444..7512,7513..8304)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7662..7681)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8475..9063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8964..8983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9217..9234
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9351..9372
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9387..9392,9393..9410,9411..9417)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9425..9441
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9430..9452
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(9449..9460,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(9449..9460,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcg cggccgcatc atgacatcag tattctgcta
       61 ttcagctaag attcttgttg tcgtcatttg atgtatccgt tcgtcggtgc aggacttcca
      121 catggcgcag tcgaaaccat gttaatctct gaagtcaaat ggtctaagaa ttcacagtat
      181 agacggactg caggtgtaaa cttatcctat atatcaccca agacgtatta ccttgtcaga
      241 ctgcatttac acaagtaatc ccacttatta tatgatgtca cgcgttataa aacggaagct
      301 tacttattgt ggaacaggtg gaatactcaa aaacattgaa aattcttaca atttctaagt
      361 aaaaacctta tgaatgtttt gtacgtatta ttattcccat tcttccattt agttgtcagt
      421 ttttcattgc tcgggaagat ttgcataaat atcgccattg accagtctac tccccagttt
      481 tttgatcatg atttttcaca acaatgatca atcaaataaa catcatatca tcttatcccg
      541 attaagcgcc ctcgcaattt tattgcctca tcttcttaat aatagaaacc atatgctcat
      601 ttttggctta gtttttgctc agttcaattt tccttattcc atcgtaagcg ttaagtctag
      661 aaggcttttt gttattcact ttttttcttt gctttttact tttctctttc ttcacgagac
      721 aaatattggt attattgctc atctctctac taataaaact caacaatttc gttgaaactt
      781 ggtctctaga gtttgatgag ttgtttgtgt tatttttgat ctaaattttc aattttgtgg
      841 aagtaaggct tgcaagatag acaaagacaa caagaaaata aacgaaggga ttgaggaaga
      901 ataaactgaa atgatcatgg ttaagaaaat aggaagaaaa tagaagaaga gcggccggct
      961 cactaggtta aattattttt gtgcttgttg tggggagcgt tgagtaagac ctagagcaag
     1021 gaacgcggtt agcaatccct gccgtttcca aatctccaga gactatgcga aattcagaag
     1081 acacgggaca tcaagacaga taggtacgga gtaagacctt caaattgttt cttctcttct
     1141 tgattactct cttgctcctc tctcgtccat tgtgctcttt cccacacact gagcaaaagc
     1201 atcgtcattg tggaaagtga gcttgatgtg gttggctcaa acatctgacc agctagactt
     1261 gtcacttgta agatatcaca tcgcacttcc caattaatct taaaatccgc ttgactcatt
     1321 tatttttgaa ttcgtttttg tttgaaattg taaacttcgg aatagttcca gaccgattca
     1381 gaactagtat gatcacctat gaaacgtgat ttctgaaata ttcttacttt tttctagaca
     1441 actcaagatc tcgatcggcc aaaattgtat tatcctcgta atcatcataa ccatttccga
     1501 tattctgatc acaacggctt tctttcgaat ctagtacgtc tcacgtacaa ctgcccacaa
     1561 acgatttcca tgaaactgat aaataattaa agactttgga cttaccaaga gttcaaacag
     1621 gttttatttc gttttctatt tttgaaaaaa aaatcattcc agttggacga caaaaacagt
     1681 tgtagaactt taataataat atttattctt gacacaaaaa aacttatcgt atttgtaagg
     1741 ggaaaataga cccataatta acaacgtttc tattagttat gcattttact gtactcatga
     1801 tattactcca ataacaaata atttatctga aatagcactt ttctccttct ccctgtgctc
     1861 caatacagtc cagacacata ttaattttca acaccacatc acgcacatct tccataggaa
     1921 attaaaaaaa aaatacatta gacttcctat tcatatcaaa tatttcacat cataatattt
     1981 ttgtttgctc atttttcaac acttttctga ttcattttat ttttgtacgt tacgatacct
     2041 acatcagtta tttttcacct gccgtcactt attttagata ttccttaatt ttgactacgt
     2101 attactcatt gttaacattt ctgatatttg tatactcaaa gccatttgga aagaatcaga
     2161 acagtgattt cagttttgat gctggccaac tagttgaata acgtgatgag gaccattgag
     2221 taattagaga aaacatgttt acgaatatag tcggctaaat tgagaaaaat acctaaataa
     2281 ctggcaataa gctcagaact gaaaaacaaa aaattgctaa ccatcccgtg aatttttgat
     2341 gtgtgcctgt ctatgccgtc atgttcatct catgtgcttt gttttcaatc cctggtctct
     2401 tgtaattttt cttttaaaaa gaagtctgct attcgtgaca aattaaaatt gaaaattcat
     2461 atatctttag agtatagccg ttttgagtca gcaacaaaca attaggtgcc acttccggga
     2521 caggcacctc tgaaataaaa ggaataagaa aatagtgaga tattcccccc tcactgatca
     2581 aacttctttt tttcccttta ctacgtttac ctatattttt attctgttag tttggtcgaa
     2641 aattggtgga ggatgtctag aatttgcagg atacaaattg agactaaaga aaattgaatt
     2701 tcttcaagtc ttctcgattt cttgatttct agtaaagctt cctttgtaac tactgtaata
     2761 gttagtgaga ataattaaat tttggcgcct gccagtcatt tctagtgaac aaatataaca
     2821 tcaatacttt gaaatctaat tttctaagta gctcgggtaa tttttttgtt cacatcactc
     2881 cttacaacac aactttcttc aaaaatttca cgtctatatt tagtttcaat ttttcaaagg
     2941 ttgaaggcgt ctagacttcg atgaatacca aaattaaagc aatcatagtt ttttaggtaa
     3001 agtttatgaa tctgcaaagt catactataa aactgatctt agaaattttt cttgcagatt
     3061 ttatcttaat atttcaaaaa acatcgttat gttcccattt tgatttttgg tgtaatggtc
     3121 gtgtttctac gaacaaaaac ggagacaccc ccaaaaattt ttgagatgct gtttttgaaa
     3181 ataataaagg cagctgtcag ctttattcat ttttctgaaa aacaattcac tgtcattttg
     3241 agctaatagc tgcaaaacca acaaacgtaa aaactcagaa agcattcaaa aatcgtaaaa
     3301 cttgagtttt tctcaagaaa tacgattaag attactgtag acaaatttat agtaaattga
     3361 attaaagtgt aatttgataa atgcatgact ttctcagatt tattcatttt ccacatataa
     3421 actctaattg aatactagga actgtgtatg agtatatcat ttgttgaaat gctacatttt
     3481 tctgttagtc tagatttttt aacgagtttg tgtcttggta attgtcgtgt gtattcattg
     3541 ttcacagctt ctaataggaa agggttattt tcaaaaaacg ttctgtactt cgctggtagg
     3601 agtactgtag ttttactatt ttttttatat aacagaacta tttgtttctg ctttgaaagc
     3661 ataattcgaa taattacgta ggattcaatt tttcttgata ctcattttct tttttgtcta
     3721 accatttcat tctgacacat aaattaccaa taacagattg attgtctgta ctttatgtta
     3781 ttttcctttc aatatttttc ctctctctta tttctaaaag cggaaatgaa aaacgactct
     3841 gagaattaat tttcttcatt tcttctgtag gcgtaactgc actttctggg atttcttttt
     3901 gctgtcataa tacgggagta cccgggattg gccaaaggac ccaaaggtat gtttcgaatg
     3961 atactaacat aacatagaac attttcagga ggacccttgg ctagcatgcc gatcaccatc
     4021 aacaacttcc gttactccga cccggttaac aacgacacca tcatcatgat ggaaccgccg
     4081 tactgcaaag gtctagacat ctactacaaa gccttcaaaa tcaccgaccg tatctggatc
     4141 gttccggaac gttacgaatt tggtactaaa ccggaagact tcaacccgcc gtcctccctg
     4201 atcgaaggtg cttccgaata ctacgacccg aactacctgc gtaccgactc cgacaaagac
     4261 cgtttcctgc agaccatggt taaactgttc aaccgtatca aaaacaacgt tgctggtgaa
     4321 gctctgctgg acaaaatcat caacgctatc ccgtacctgg gtaactccta ctccctgctg
     4381 gacaaattcg acaccaactc caactccgtt tccttcaacc tgctggaaca ggatccgtcc
     4441 ggtgctacca ccaaatccgc tatgctgacc aacctgatca tcttcggtcc gggtccggtt
     4501 ctgaacaaaa acgaagttcg tggtatcgtt ctgcgtgttg acaacaaaaa ctacttcccg
     4561 tgccgtgacg gtttcggttc catcatgcag atggctttct gcccggaata cgtaccgacc
     4621 ttcgacaacg ttatcgaaaa catcacctcc ctgaccatcg gtaaatccaa atacttccag
     4681 gacccggctc tgctgctgat gcacgaactg atccacgttc tgcacggtct gtacggcatg
     4741 caggtttcct cccacgaaat catcccgtcc aaacaggaaa tctacatgca gcacacctac
     4801 ccgatctccg ctgaagagct cttcaccttc ggtggtcagg acgctaacct gatctccatc
     4861 gatatcaaaa acgacctgta cgaaaaaacc ctgaacgact acaaagctat cgctaacaaa
     4921 ctgtcccagg ttacctcctg caacgacccg aacatcgaca tcgactccta caaacagatc
     4981 tatcagcaga agtatcagtt tgataaggat tccaacggcc agtatatagt gaacgaggat
     5041 aagtttcaga tactgtataa ctccataatg tatggcttta ctgagataga gctgggcaag
     5101 aagtttaaca taaagactcg cttaagctat ttttccatga accacgatcc tgtgaagata
     5161 cctaacctgc tggatgatac tatatataac gatactgagg gctttaacat agagtccaag
     5221 gatctgaagt ccgagtataa gggccagaac atgagggtga acactaacgc ctttaggaac
     5281 gtcgacggtt ccggtctggt ttccaaactg atcggtctgt gcaaaaaaat catcccgccg
     5341 accaacatcc gtgaaaacct gtacaaccgt accgctaggg taccggtaga aaaaatgctc
     5401 gagatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     5461 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     5521 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     5581 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     5641 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     5701 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     5761 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     5821 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     5881 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     5941 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     6001 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     6061 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagaattc
     6121 caactgagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata ataggggccg
     6181 ctgtcatcag agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc
     6241 agcatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg
     6301 ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact tctcttaatt
     6361 tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa
     6421 tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata
     6481 attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat
     6541 aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt
     6601 ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat
     6661 catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt
     6721 atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg tcaagatttc
     6781 gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc aagaaagatc
     6841 ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata atttgaaagt
     6901 ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa tataccaaac
     6961 ataactgttt cctactagtc ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga
     7021 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     7081 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     7141 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     7201 accgcacaga tgcgtaagga gaaaataccg catcaggcgg ccttaattaa gggcctcgtg
     7261 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7321 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7381 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7441 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7501 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7561 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7621 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7681 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7741 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7801 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7861 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7921 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7981 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8041 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8101 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8161 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8221 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8281 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8341 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8401 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8461 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8521 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8581 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8641 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8701 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8761 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8821 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8881 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8941 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9001 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9061 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9121 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9181 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9241 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9301 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     9361 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     9421 ggaattgtga gcggataaca atttcacaca ggaaacagct
//
