LOCUS       sequence-408460-        8098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pflp-18 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVA 
            and other neurons expression of GtACR2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Meng J, Ahamed T, Yu B, Hung W, Ei Mouridi S, Wang Z, Zhang Y, Wen 
            Q, Boulin T, Gao S, Zhen M
  TITLE     A tonically active master neuron modulates mutually exclusive motor 
            states at two timescales.
  JOURNAL   Sci Adv. 2024 Apr 12;10(15):eadk0002. doi: 10.1126/sciadv.adk0002. 
            Epub 2024 Apr 10.
  PUBMED    38598630
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-04-12"; volume: "10(15):eadk0002. doi"; pages: " 
            10.1126/sciadv.adk0002. Epub 2024 Apr 10"
COMMENT     Sequence Label: pJH4562
FEATURES             Location/Qualifiers
     source          1..8098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(429..440)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1558..1591)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      2468..2791
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(2795..2828)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(5538..5560)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5660..5679
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5757..5775)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5843..5947
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5948..6016,6017..6808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6166..6185)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6979..7567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7468..7487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7721..7738
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7855..7876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7891..7896,7897..7914,7915..7921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7929..7945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7934..7956
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7953..7969
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7953..7969
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcatgc agcttagccg gaatagggtc acgaaaacat gaaaataaag
       61 cttgaaccta ttgttatgat tttcaatgcc accttttgca gtttaatata cactattact
      121 acttctcagt tcaagttcac ttctgagacg gaacctttat tttctaactt tctttaatct
      181 tactttttat gagccgaacg ttctattttg taaaatgatc agatacaagc ccaaagctaa
      241 ttttgccaat aagtgttagt agatcaattg aattacaata taatgacctg ggcggaatag
      301 atttttgcac tgatttttct ccaagcttac tctatgaata ttcatagatc atcttcaaag
      361 gtagcgtgtg ttgtagttgt gttttggcgg gttcgtgtta tttgactcag ctcaagtaga
      421 tgttgtagtc ttccctcaat caacgcgtgg gcttgaccct ctttaaattg agtggaaacc
      481 agccaatcta tttgaaatct acccccgaaa tgagattcga cgatgacacg acgtgtgtgt
      541 tacgtgcgct gagaatttag gaaaaaaaag ataatcttct tttcgttagc tgcgccacaa
      601 cctgtgcaga taaactccgc acaattgaag ttcttactgg tcccacccga tttattcaat
      661 tgtattgtgt ctttttttct tatttctcta caactctaac tttcttcaac tattctctac
      721 atcagtgctt ttttccacca tgaaaaaaga aatgacgtcg ttgtttctta ggaggggcgg
      781 agcctcaact caatggtccg ttcgaataga tcccacccct catataaata ggactgaaac
      841 caaagaagtt tttaaatttt cagcttaccg tgtttctcaa ttttcaaaat agtctttttt
      901 gtgtctgaac gggctgatca attatgtgtt tttttgaatg aaaaatcttc ctcccaagcc
      961 ttaaaattcc ttccaaagat cgaaagttat gtctattcaa gttacttctt cttagatcaa
     1021 atattaaaca aaaaaatacg ccatcattct ctcacattta gtaattcaaa atttcgattt
     1081 gttttcgaac ttttgaattt cccgcctagg aagtgacatg agtgaaatct cattagataa
     1141 atgtggcact caccgatgac actattttca aataaggggg gggggtgttt cgaatacctt
     1201 gtacacgcgt ttccgcaaaa ccacacaccc cgagttcctc agcttcttcc cctcgaccca
     1261 tgagccacgt caagatgtca gcgtgtcact tttcattgct tcaccttcta cggtaacgag
     1321 aaaaggcggg gaatccagga tctggtgtga cttcttcctt cttcgtatct tcctaaaaat
     1381 gttcgtttac tttttagcga gtgtcatttg atgggatgtc gttcttgagg aaagaggcta
     1441 gaagcttaat actctcatat ttcatattat tggcatcaag acgtttatta tttaactaca
     1501 ataacgattg ctttaacaac taaaaataat aatttcaggg ttagacggat ccccgggata
     1561 acttcgtata gcatacatta tacgaagtta tatggtctcc aagggagagg agctcttcac
     1621 cggagtcgtc ccaatcctcg tcgagctcga cggagacgtc aacggacaca agttctccgt
     1681 ccgtggagag ggagagggag acgccaccaa cggaaagctc accctcaagt tcatctgcac
     1741 caccggaaag ctcccagtcc cagtaagttt aaacatatat atactaacta accctgatta
     1801 tttaaatttt cagtggccaa ccctcgtcac caccctctcc cacggagtcc agtgcttcgc
     1861 ccgttaccca gaccacatga agcagcacga cttcttcaag tccgccatgc cagagggata
     1921 cgtccaggag cgtaccatct tcttcaagga cgacggaacc tacaagagta agtttaaaca
     1981 tgattttact aactaactaa tctgatttaa attttcagcc cgtgccgagg tcaagttcga
     2041 gggagacacc ctcgtcaacc gtatcgagct caagggagtc gacttcaagg aggacggaaa
     2101 catcctcgga cacaagctcg agtacaactt caactcccac aacatctaca tcatggccgt
     2161 caagcagaag aacggaatca aggtaagttt aaacatgatt ttactaacta actaatctga
     2221 tttaaatttt caggtcaact tcaagatccg tcacaacgtc gaggacggat ccgtccagct
     2281 cgccgaccac taccagcaga acaccccaat cggagacgga ccagtcctcc tcccagactc
     2341 ccactacctc tccacccagt ccgtcctctc caaggaccca aacgagaagc gtgaccacat
     2401 ggtcctcctc gagttccgta ccgccgccgg aatcaccctc ggaatggacg agctctacaa
     2461 gtgataaatg caaaatcctt tcaagcattc ccttcttctc tatcactctt ctttcttttt
     2521 gtcaaaaaat tctctcgcta atttatttgc ttttttaatg ttattatttt atgacttttt
     2581 atagtcactg aaaagtttgc atctgagtga agtgaatgct atcaaaatgt gattctgtct
     2641 gatgtacttt cacaatctct cttcaattcc attttgaagt gctttaaacc cgaaaggttg
     2701 agaaaaatgc gagcgctcaa atatttgtat tgtgttcgtt gagtgaccca acaaaaagag
     2761 gaaactttat tgtgccgcca agaaaaaagt ctcaataact tcgtatagca tacattatac
     2821 gaagttatgc tagcatggcc tcccaggtcg tgtacggcga gtgggccagc acccacacag
     2881 agtgctacaa catgagcagg atcgacagca ccttcgtgag cctgctgcag ctggtgtggg
     2941 ccgtggtgag cggatgccag accatcttca tgatcagcag ggcccccaag gtgccctggg
     3001 agagcgtgta cctgcccttc gtggagtcca tcacatacgc cctggccagc accggcaatg
     3061 gcacactgca gatgagggac ggcaggttct ttccctggtc caggatggcc agctggctgt
     3121 gcacatgccc cattatgctg ggccagatct ccaacatggc tctggtgaag tacaagagca
     3181 tccctctgaa ccccatcgcc caggccgcca gcattatcag ggtggtgatg ggcattaccg
     3241 ctaccatctc ccccgccgag tacatgaagt ggctgttctt cttcttcggc gctacctgcc
     3301 tggtcttcga atacagcgtg gtgttcacca tcttccaggt gggactgtac ggcttcgagt
     3361 ccgtgggcac ccctctggcc cagaaggtgg tggtgaggat caagatgctg aggctgatct
     3421 tcttcatcgc ctggaccatg ttccccatcg tgtggctgat ttcccccacc ggcgtgtgcg
     3481 tgatccacga gaacgtgagc gccatcctgt atctgctcgc cgacggcctg tgcaagaaca
     3541 cctacggcgt gatcctgtgg tccaccgcct ggggagtgct cgagggaaag tgggacccgg
     3601 cttgcctccc tggccaggag aagcctgagg ccgacgaccc cttcggactg aaccacgaga
     3661 agaatgcccc ccccaacgac gaggtcaaca tcagaatgtt cggcagggcg gccgccgcgg
     3721 taccgggagg aggaaccatg gtctcaaagg gtgaagaaga taacatggca attattaaag
     3781 agtttatgcg tttcaaggtg catatggagg gatctgtcaa tgggcatgag tttgaaattg
     3841 aaggtgaagg agaaggccga ccatatgagg gaacacaaac cgcaaaacta aaggtaagtt
     3901 taaacatata tatactaact aaccctgatt atttaaattt tcaggtaact aaaggcggac
     3961 cattaccatt cgcctgggac atcctctctc cacagttcat gtatggaagt aaagcttatg
     4021 ttaaacatcc ggcagatata ccagattatt tgaaactttc attcccggag ggttttaagt
     4081 gggaacgcgt aatgaatttt gaagacggag gagttgttac agtgacgcaa gactcaaggt
     4141 aagtttaaac agttcggtac taactaacca tacatattta aattttcagc ctccaagatg
     4201 gagaatttat ttataaagtc aaacttcgag gaacgaattt cccctcggat ggatctgtta
     4261 tgcagaagaa gactatggga tgggaagctt caagtgaaag aatgtaccct gaagacggtg
     4321 ctcttaaggg agagattaaa caacgtctta aattgaaaga tggaggacat tacgatgctg
     4381 aggtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt caggtgaaga
     4441 caacttacaa agccaaaaaa ccagttcagc tgccaggagc gtacaatgtt aatattaaac
     4501 tggatatcac ctcccacaac gaggattaca ctatcgttga gcaatatgaa agagctgaag
     4561 ggcggcactc gacaggtggc atggatgaat tgtataagtt ctgctacgag aacgaggtct
     4621 aagaattcca actgagcgcc ggtcgctacc attaccaact tgtctggtgt caaaaataat
     4681 aggggccgct gtcatcagag taagtttaaa ctgagttcta ctaactaacg agtaatattt
     4741 aaattttcag catctcgcgc ccgtgcctct gacttctaag tccaattact cttcaacatc
     4801 cctacatgct ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaacttc
     4861 tcttaatttc tttgtttttt agcttctttt aagtcacctc taacaatgaa attgtgtaga
     4921 ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat
     4981 taataataat tctatcccaa aatctacaca atgttctgtg tacacttctt atgtttttta
     5041 cttctgataa atttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata
     5101 tgttacgttt cagtttatga ccgcaatttt tatttcttcg cacgtctggg cctctcatga
     5161 cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt ttggaatttt tcaatcaagt
     5221 gaaagtttat gaaattaatt ttcctgcttt tgctttttgg ggtttcccct attgtttgtc
     5281 aagatttcga ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa
     5341 gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat
     5401 ttgaaagtgg agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata
     5461 taccaaacat aactgtttcc tactagtcgg ccgtacgggc cctttcgtct cgcgcgtttc
     5521 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     5581 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     5641 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     5701 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcggcc ttaagggcct
     5761 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5821 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5881 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5941 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6001 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     6061 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     6121 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     6181 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     6241 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     6301 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     6361 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     6421 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6481 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6541 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6601 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6661 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6721 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6781 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6841 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6901 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6961 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7021 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7081 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7141 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     7201 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     7261 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     7321 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     7381 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     7441 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7501 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7561 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7621 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     7681 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     7741 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     7801 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     7861 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     7921 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     7981 caagctgtaa gtttaaacat gatcttacta actaactatt ctcatttaaa ttttcagagc
     8041 ttaaaaatgg ctgaaatcac tcacaacgat ggatacgcta acaacttgga aatgaaat
//
