LOCUS       sequence-408496-       10848 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knocking-down OPA1 in mouse cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Suga S, Nakamura K, Nakanishi Y, Humbel BM, Kawai H, Hirabayashi Y
  TITLE     An interactive deep learning-based approach reveals mitochondrial 
            cristae topologies.
  JOURNAL   PLoS Biol. 2023 Aug 31;21(8):e3002246. doi: 
            10.1371/journal.pbio.3002246. eCollection 2023 Aug.
  PUBMED    37651352
REFERENCE   2  (bases 1 to 10848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-08-31"; volume: "21(8):e3002246. doi"; pages: " 
            10.1371/journal.pbio.3002246. eCollection 2023 Aug"
COMMENT     Sequence Label: pH1-shOPA1-pSyn-tdtomato
FEATURES             Location/Qualifiers
     source          1..10848
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2605..2819
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     2740..2759
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     regulatory      4022..4031
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4028..5458
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    5536..6124
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5589..5609)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6007..6018)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6127..6143)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6128..6144)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6653..6833
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6859..6876)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6865..7089
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7135..7563
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7222..7241)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7432..7453
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7572..7592)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7577..7906
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7757..7892
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7819..7838
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7954..8001
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8020..8394
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8524..8645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8561..8580)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8615..8634
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8694..8710)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8694..8710)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8707..8729)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8718..8734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8742..8748,8749..8766,8767..8772))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8787..8808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8925..8942)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9096..9684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9176..9195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9855..10646,10647..10715))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10478..10497
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10716..10820)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagcgaacgc tgacgtcatc aacccgctcc aaggaatcgc
     2641 gggcccagtg tcactaggcg ggaacaccca gcgcgcgtgc gccctggcag gaagatggct
     2701 gtgagggaca ggggagtggc gccctgcaat atttgcatgt cgctatgtgt tctgggaaat
     2761 caccataaac gtgaaatgtc tttggatttg ggaatcttat aagttctgta tgagaccacc
     2821 tcgagccggc atggaagaag aaccatattt ctcgagaaat atggttcttc ttccatgttt
     2881 ttgtctagag ttaacttaat taagggtttt ggctacgtcc agagcagagg aatgagggca
     2941 tgtagactaa atatgttcgt gtggaagagg ctgaatacac atcagagtta gtgctgcagg
     3001 aaatgcttct gcattgcata cccagagttt ccttgctcat ctgagagcat gtgttttttc
     3061 cagatgtgtg tacttgtgtg agattctctg ggtgtgtgtc aatgtgttgc ctgaacgtgc
     3121 attgctcaat atgctcatgt gtgttaccct gggcttgtac atctacatat atacctggat
     3181 gcccgtgtgt tctgtgatgt acatataccc tgtgtcattc cttgtttttc tatttgtgtt
     3241 attccatgtg ttccttcagg ctctcactac ccaagtgtcc acctccgcct gtctggtgat
     3301 gtttacgcta ccccgtgctc ttttctttgc ctgacagtgt tgtcgtggaa gacatctcgc
     3361 caggaacact gcagtaagga gaatttctag ttttatgttc ccctccgagt atgcttctat
     3421 cccgaccctc aaccccaaaa tgccttcaga ggtgaaaatc aacactggaa acacaagtat
     3481 ctgggaaggg taacaatgca agttagcctg aggatttagg aggaggctga aaaacagagt
     3541 aggagcctta ctacgggtcc agaccctacg gacaagaacc cccactccca ctccccaaat
     3601 tgcgcattcc ctcccccatc agagggggag gggaagagga tgcagcgcgg cgcggcgcgt
     3661 gcgcactgtc ggatttagta ccgcggacag agccttcgcc cccgctgccg gcgcgcgcca
     3721 ccacctcccc agcaccaaag gcgggctgac gtcactctcc agccctcccc aaactcccct
     3781 acctcaccgc cttggtcgcg tccgtgcagc ggtgagtcca gtcgggccgc accacaagag
     3841 gtgcaagata ggggggtgca ggcgcgacca tacgctctgc ggcggcagag cctcagcgct
     3901 gcctcagtct gcagcgggca gcagaggagt cgcgtcgtgc cagagagcgc cgccgtgctc
     3961 ctgagcccct tgcgctccgc ccccgcggcc caccgaccca ctgccccttg gatccgaatt
     4021 cgccaccatg gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg
     4081 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     4141 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     4201 ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc
     4261 cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     4321 gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac
     4381 gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca
     4441 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     4501 gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     4561 caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac
     4621 caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc
     4681 cgagggccgc caccacctgt tcctggggca tggcaccggc agcaccggca gcggcagctc
     4741 cggcaccgcc tcctccgagg acaacaacat ggccgtcatc aaagagttca tgcgcttcaa
     4801 ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     4861 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
     4921 cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca
     4981 ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg
     5041 cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga
     5101 cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt
     5161 aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
     5221 cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt
     5281 ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt
     5341 ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     5401 gcgctccgag ggccgccacc acctgttcct gtacggcatg gacgagctgt acaagtaagc
     5461 tagcccacgc gcgtggtgta caagtaaagc ggccgcgact ctagtccctc ccaattcgat
     5521 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5581 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5641 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5701 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5761 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5821 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5881 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     5941 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6001 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6061 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6121 ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag
     6181 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     6241 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6301 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6361 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     6421 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     6481 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     6541 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     6601 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     6661 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6721 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6781 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     6841 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     6901 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6961 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     7021 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     7081 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     7141 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7201 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7261 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7321 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7381 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7441 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7501 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7561 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7621 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     7681 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     7741 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7801 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     7861 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     7921 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     7981 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     8041 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     8101 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     8161 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     8221 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     8281 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     8341 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     8401 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     8461 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     8521 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8581 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8641 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     8701 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8761 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8821 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8881 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8941 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9001 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9061 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9121 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9181 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9241 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9301 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9361 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9421 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9481 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9541 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9601 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9661 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9721 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9781 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9841 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9901 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9961 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    10021 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    10081 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    10141 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10201 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10261 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10321 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10381 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10441 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10501 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10561 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10621 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10681 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10741 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10801 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgac
//
