LOCUS       sequence-408507-        6362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-mediated expression of GCaMP8f under the CamKIIa promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6362)
  TITLE     Looger Lab GCaMP8 plasmids
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAV-CamKIIa-jGCaMP8f-WPRE
FEATURES             Location/Qualifiers
     source          1..6362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(185..773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(265..284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(944..1735,1736..1804))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1567..1586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1805..1909)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1935..2390)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2072..2093)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2284..2303
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2462..2591
                     /label=AAV2 ITR
     promoter        2656..3944
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             3983..4000
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     4282..4303
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4409..4430)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4670..4689)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    5261..5849
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5314..5334)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5732..5743)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5852..5868)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5853..5869)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5898..6019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5909..5928)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5963..5982
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   6181..6310
                     /label=AAV2 ITR
ORIGIN
        1 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
       61 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      121 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      181 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      241 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      301 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      361 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      421 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      481 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      541 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      601 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      661 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      721 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      781 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      841 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      901 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      961 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1021 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1081 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1141 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1201 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1261 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1321 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1381 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1441 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1501 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1561 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1621 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1681 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1741 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1801 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1861 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1921 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     1981 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     2041 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     2101 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     2161 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     2221 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     2281 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     2341 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     2401 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     2461 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
     2521 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
     2581 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc
     2641 taggaagatc tttaacatta tggccttagg tcacttcatc tccatggggt tcttcttctg
     2701 attttctaga aaatgagatg ggggtgcaga gagcttcctc agtgacctgc ccagggtcac
     2761 atcagaaatg tcagagctag aacttgaact cagattacta atcttaaatt ccatgccttg
     2821 ggggcatgca agtacgatat acagaaggag tgaactcatt agggcagatg accaatgagt
     2881 ttaggaaaga agagtccagg gcagggtaca tctacaccac ccgcccagcc ctgggtgagt
     2941 ccagccacgt tcacctcatt atagttgcct ctctccagtc ctaccttgac gggaagcaca
     3001 agcagaaact gggacaggag ccccaggaga ccaaatcttc atggtccctc tgggaggatg
     3061 ggtggggaga gctgtggcag aggcctcagg aggggccctg ctgctcagtg gtgacagata
     3121 ggggtgagaa agcagacaga gtcattccgt cagcattctg ggtctgtttg gtacttcttc
     3181 tcacgctaag gtggcggtgt gatatgcaca atggctaaaa agcagggaga gctggaaaga
     3241 aacaaggaca gagacagagg ccaagtcaac cagaccaatt cccagaggaa gcaaagaaac
     3301 cattacagag actacaaggg ggaagggaag gagagatgaa ttagcttccc ctgtaaacct
     3361 tagaacccag ctgttgccag ggcaacgggg caatacctgt ctcttcagag gagatgaagt
     3421 tgccagggta actacatcct gtctttctca aggaccatcc cagaatgtgg cacccactag
     3481 ccgttaccat agcaactgcc tctttgcccc acttaatccc atcccgtctg ttaaaagggc
     3541 cctatagttg gaggtggggg aggtaggaag agcgatgatc acttgtggac taagtttgtt
     3601 cgcatcccct tctccaaccc cctcagtaca tcaccctggg ggaacagggt ccacttgctc
     3661 ctgggcccac acagtcctgc agtattgtgt atataaggcc agggcaaaga ggagcaggtt
     3721 ttaaagtgaa aggcaggcag gtgttgggga ggcagttacc ggggcaacgg gaacagggcg
     3781 tttcggaggt ggttgccatg gggacctgga tgctgacgaa ggctcgcgag gctgtgagca
     3841 gccacagtgc cctgctcaga agccccaagc tcgtcagtca agccggttct ccgtttgcac
     3901 tcaggagcac gggcaggcga gtggccccta gttctggggg cagcgaattc aagctgctag
     3961 caaggatcca cccgccacca tgcatcatca ccatcatcac acgcgtcgca agaagacctt
     4021 caaggaggtg gccaccgccg tgaagatcat cgcccgcctg atgggcctga agatcaacgt
     4081 ctatatcaag gccgacaagc agaagaacgg catcaaggcg aacttccaca tccgccacaa
     4141 catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc ccatcggcga
     4201 cggccccgtg ctgctgcccg acaaccacta cctgagcgtg gagtccaaac tttcgaaaga
     4261 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     4321 tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatggtga gcaagggcga
     4381 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     4441 caagttcagc gtgtccggcg agggtgaggg cgatgccacc tacggcaagc tgaccctgaa
     4501 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     4561 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4621 gtccgccatg cccgaaggct acatccagga gcgcaccatc ttcttcaagg acgacggcaa
     4681 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4741 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacct
     4801 gccggaccaa ctgactgaag agcagatcgc agaatttaaa gaggctttct ccctatttga
     4861 caaggacggg gatgggacaa taacaaccaa ggagctgggg acggtgatgc ggtctctcgg
     4921 actgaacccc acagaagcag agctgcagga catgatcaat gaagtagatg ccgacggtga
     4981 cggcacaatc gacttccctg agttcctgac aatgatggca agaaaaatga aatacaggga
     5041 cacggaagaa gaaattagag aagcgttcgg tgtgtttgat aaggatggca atggctacat
     5101 cagtgcagca gagcttcgcc acgtgatgac aaaccttgga gagaagttaa cagatgaaga
     5161 ggttgatgaa atgatcaggg aagcagacat cgatggggat ggtcaggtaa actacgaaga
     5221 gtttgtacaa atgatgacag cgaagtaaaa gcttatcgat aatcaacctc tggattacaa
     5281 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5341 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5401 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     5461 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     5521 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     5581 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     5641 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     5701 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     5761 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     5821 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgac ccgggcggcc
     5881 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     5941 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6001 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6061 ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
     6121 ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca
     6181 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     6241 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     6301 gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6361 gc
//
