LOCUS       sequence-408525-       10113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control for pH1-shOPA1-pSyn-tdtomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10113)
  AUTHORS   Suga S, Nakamura K, Nakanishi Y, Humbel BM, Kawai H, Hirabayashi Y
  TITLE     An interactive deep learning-based approach reveals mitochondrial 
            cristae topologies.
  JOURNAL   PLoS Biol. 2023 Aug 31;21(8):e3002246. doi: 
            10.1371/journal.pbio.3002246. eCollection 2023 Aug.
  PUBMED    37651352
REFERENCE   2  (bases 1 to 10113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-08-31"; volume: "21(8):e3002246. doi"; pages: " 
            10.1371/journal.pbio.3002246. eCollection 2023 Aug"
COMMENT     Sequence Label: pH1-shControl-pSyn-tdtomato
FEATURES             Location/Qualifiers
     source          1..10113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(77..94)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(248..836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(328..347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1007..1798,1799..1867))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1630..1649
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1868..1972)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2047..2066)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2238..2617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2619..2817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2772..2792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2835..3015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3062..3187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3680..3913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4098..4142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4440..4557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4605..4819
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     4740..4759
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     regulatory      6013..6022
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6019..6720
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(6152..6170)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6420..6439
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6614..6637
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    6801..7389
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6854..6874)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7272..7283)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7392..7408)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7393..7409)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7918..8098
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8124..8141)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8130..8354
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8400..8828
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8487..8506)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8697..8718
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8837..8857)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8842..9171
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9022..9157
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9084..9103
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9219..9266
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9285..9659
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9789..9910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9826..9845)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9880..9899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9959..9975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9959..9975)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9972..9994)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9983..9999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10007..10013,10014..10031,10032..10037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10052..10073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
       61 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      661 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1081 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1141 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1201 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1261 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1321 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1381 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1441 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1501 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1561 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1621 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1681 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1741 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1801 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1861 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     1921 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     1981 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
     2041 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
     2101 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
     2161 cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg
     2221 ggccagatat acgcgttgac attgattatt gactagttat taatagtaat caattacggg
     2281 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2341 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2401 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2461 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2521 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     2581 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2641 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2701 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2761 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc
     2821 gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     2881 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     2941 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3001 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     3061 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     3121 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     3181 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     3241 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     3301 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     3361 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     3421 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     3481 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     3541 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     3601 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     3661 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     3721 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     3781 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     3841 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     3901 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     3961 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     4021 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     4081 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     4141 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     4201 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     4261 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     4321 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     4381 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
     4441 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4501 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     4561 gtttattaca gggacagcag agatccagtt tggttaattc tagcgaacgc tgacgtcatc
     4621 aacccgctcc aaggaatcgc gggcccagtg tcactaggcg ggaacaccca gcgcgcgtgc
     4681 gccctggcag gaagatggct gtgagggaca ggggagtggc gccctgcaat atttgcatgt
     4741 cgctatgtgt tctgggaaat caccataaac gtgaaatgtc tttggatttg ggaatcttat
     4801 aagttctgta tgagaccacc tcgagccgca ggtatgcacg cgttcaagag acgcgtgcat
     4861 acctgcggtt tttgtctaga gttaacttaa ttaagggttt tggctacgtc cagagcagag
     4921 gaatgagggc atgtagacta aatatgttcg tgtggaagag gctgaataca catcagagtt
     4981 agtgctgcag gaaatgcttc tgcattgcat acccagagtt tccttgctca tctgagagca
     5041 tgtgtttttt ccagatgtgt gtacttgtgt gagattctct gggtgtgtgt caatgtgttg
     5101 cctgaacgtg cattgctcaa tatgctcatg tgtgttaccc tgggcttgta catctacata
     5161 tatacctgga tgcccgtgtg ttctgtgatg tacatatacc ctgtgtcatt ccttgttttt
     5221 ctatttgtgt tattccatgt gttccttcag gctctcacta cccaagtgtc cacctccgcc
     5281 tgtctggtga tgtttacgct accccgtgct cttttctttg cctgacagtg ttgtcgtgga
     5341 agacatctcg ccaggaacac tgcagtaagg agaatttcta gttttatgtt cccctccgag
     5401 tatgcttcta tcccgaccct caaccccaaa atgccttcag aggtgaaaat caacactgga
     5461 aacacaagta tctgggaagg gtaacaatgc aagttagcct gaggatttag gaggaggctg
     5521 aaaaacagag taggagcctt actacgggtc cagaccctac ggacaagaac ccccactccc
     5581 actccccaaa ttgcgcattc cctcccccat cagaggggga ggggaagagg atgcagcgcg
     5641 gcgcggcgcg tgcgcactgt cggatttagt accgcggaca gagccttcgc ccccgctgcc
     5701 ggcgcgcgcc accacctccc cagcaccaaa ggcgggctga cgtcactctc cagccctccc
     5761 caaactcccc tacctcaccg ccttggtcgc gtccgtgcag cggtgagtcc agtcgggccg
     5821 caccacaaga ggtgcaagat aggggggtgc aggcgcgacc atacgctctg cggcggcaga
     5881 gcctcagcgc tgcctcagtc tgcagcgggc agcagaggag tcgcgtcgtg ccagagagcg
     5941 ccgccgtgct cctgagcccc ttgcgctccg cccccgcggc ccaccgaccc actgcccctt
     6001 ggatccgaat tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc
     6061 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     6121 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     6181 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     6241 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     6301 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     6361 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     6421 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     6481 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     6541 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     6601 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     6661 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag
     6721 taagctagcc cacgcgcgtg gtgtacaagt aaagcggccg cgactctagt ccctcccaat
     6781 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     6841 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6901 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6961 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7021 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7081 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7141 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     7201 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7261 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7321 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7381 cctccccgca tcgataccgt cgacctcgat cgagacctag aaaaacatgg agcaatcaca
     7441 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     7501 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     7561 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7621 cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg
     7681 cagaactaca caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag
     7741 ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg
     7801 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg
     7861 tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg
     7921 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7981 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8041 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg
     8101 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     8161 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     8221 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     8281 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     8341 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     8401 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8461 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8521 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8581 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8641 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     8701 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     8761 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     8821 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     8881 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     8941 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     9001 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9061 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     9121 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     9181 ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa tcatcggcat
     9241 agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc aagttgacca
     9301 gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc
     9361 ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg
     9421 tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg
     9481 tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact
     9541 tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt
     9601 tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac
     9661 acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     9721 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     9781 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9841 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9901 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     9961 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    10021 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    10081 tgcgctcact gcccgctttc cagtcgggaa acc
//
