LOCUS       sequence-408527-       11274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Episomal yeast plasmid expressing the Ercas12a  and ErCas12a gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Bennis NX, Anderson JP, Kok SMC, Daran JG
  TITLE     Expanding the genome editing toolbox of Saccharomyces cerevisiae 
            with the endonuclease ErCas12a.
  JOURNAL   FEMS Yeast Res. 2023 Jan 4;23:foad043. doi: 10.1093/femsyr/foad043.
  PUBMED    37791490
REFERENCE   2  (bases 1 to 11274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2023-01-4"; volume: "23:foad043. doi"; pages: " 
            10.1093/femsyr/foad043"
COMMENT     Sequence Label: pUDP293
FEATURES             Location/Qualifiers
     source          1..11274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            61..1638
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     CDS             409..1437
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             409..1437
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             6248..6268
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        7187..7853
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     7723..7746
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     RBS             8072..8083
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             8090..8803
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      8815..8886
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8902..8929
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     ncRNA           8962..9029
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             complement(join(9515..10306,10307..10375))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10138..10157
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10376..10480)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10583..11171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11072..11091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actatatgtg aaggcatggc tatggcacgg cagacattcc gccagatcat caataggcac
       61 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
      121 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
      181 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag
      241 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
      301 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
      361 ttgctaggat acagttctca catcacatcc gaacataaac aaggatccat gggtaaaaag
      421 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtgtcc
      481 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
      541 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
      601 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattt
      661 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
      721 cctgaaaccg aactgcccgc tgttctgcaa ccggtcgcgg aggccatgga tgcgatcgct
      781 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
      841 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
      901 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
      961 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
     1021 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
     1081 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
     1141 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
     1201 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
     1261 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
     1321 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
     1381 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaataactc
     1441 gaggacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt
     1501 gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc
     1561 atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga
     1621 atgctggtcg ctatactgga gctctcgaga accctcacct ttcgagagga cgatgcccgt
     1681 gtctaaatga ttcgaccagc ctaagaatgt tcaacactgg ccgataattg cagacgaacg
     1741 gtgagtaagg aaagagtgag gaactatcgc atacctgcat ttaaagatgc cgatttgggc
     1801 gcgaatcctt tattttggct tcaccctcat actattatca gggccagaaa aaggaagtgt
     1861 ttccctcctt cttgaattga tgttaccctc ataaagcacg tggcctctta tcgagaaaga
     1921 aattaccgtc gctcgtgatt tgtttgcaaa aagaacaaaa ctgaaaaaac ccagacacgc
     1981 tcgacttcct gtcatcctat tgattgcagc ttccaatttc gtcacacaac aaggtcctag
     2041 cgacggctca caggttttgt aacaagcaat cgaaggttct ggaatggcgg gaaagggttt
     2101 agtaccacat gctatgatgc ccactgtgat ctccagagca aagttcgttc gatcgtactg
     2161 ttactctctc tctttcaaac agaattgtcc gaatcgtgtg acaacaacag cctgttctca
     2221 cacactcttt tcttctaacc aagggggtgg tttagtttag tagaacctcg tgaaacttac
     2281 atttacatat atataaactt gcataaattg gtcaatgcaa gaaatacata tttggtcttt
     2341 tctaattcgt agtttttcaa gttcttagat gctttctttt tctctttttt acagatcatc
     2401 aaggaagtaa ttatctactt tttacaacaa atataaaaca agatctatga acaacggtac
     2461 taacaacttc caaaacttca tcggtatctc ttctttgcaa aagactttga gaaacgcttt
     2521 gatcccaact gaaactactc aacaattcat cgttaagaac ggtatcatca aggaagacga
     2581 attgagaggt gaaaacagac aaatcttgaa ggacatcatg gacgactact acagaggttt
     2641 catctctgaa actttgtctt ctatcgacga catcgactgg acttctttgt tcgaaaagat
     2701 ggaaatccaa ttgaagaacg gtgacaacaa ggacactttg atcaaggaac aaactgaata
     2761 cagaaaggct atccacaaga agttcgctaa cgacgacaga ttcaagaaca tgttctctgc
     2821 taagttgatc tctgacatct tgccagaatt cgttatccac aacaacaact actctgcttc
     2881 tgaaaaggaa gaaaagactc aagttatcaa gttgttctct agattcgcta cttctttcaa
     2941 ggactacttc aagaacagag ctaactgttt ctctgctgac gacatctctt cttcttcttg
     3001 tcacagaatc gttaacgaca acgctgaaat cttcttctct aacgctttgg tttacagaag
     3061 aatcgttaag tctttgtcta acgacgacat caacaagatc tctggtgaca tgaaggactc
     3121 tttgaaggaa atgtctttgg aagaaatcta ctcttacgaa aagtacggtg aattcatcac
     3181 tcaagaaggt atctctttct acaacgacat ctgtggtaag gttaactctt tcatgaactt
     3241 gtactgtcaa aagaacaagg aaaacaagaa cttgtacaag ttgcaaaagt tgcacaagca
     3301 aatcttgtgt atcgctgaca cttcttacga agttccatac aagttcgaat ctgacgaaga
     3361 agtttaccaa tctgttaacg gtttcttgga caacatctct tctaagcaca tcgttgaaag
     3421 attgagaaag atcggtgaca actacaacgg ttacaacttg gacaagatct acatcgtttc
     3481 taagttctac gaatctgttt ctcaaaagac ttacagagac tgggaaacta tcaacactgc
     3541 tttggaaatc cactacaaca acatcttgcc aggtaacggt aagtctaagg ctgacaaggt
     3601 taagaaggct gttaagaacg acttgcaaaa gtctatcact gaaatcaacg aattggtttc
     3661 taactacaag ttgtgttctg acgacaacat caaggctgaa acttacatcc acgaaatctc
     3721 tcacatcttg aacaacttcg aagctcaaga attgaagtac aacccagaaa tccacttggt
     3781 tgaatctgaa ttgaaggctt ctgaattgaa gaacgttttg gacgttatca tgaacgcttt
     3841 ccactggtgt tctgttttca tgactgaaga attggttgac aaggacaaca acttctacgc
     3901 tgaattggaa gaaatctacg acgaaatcta cccagttatc tctttgtaca acttggttag
     3961 aaactacgtt actcaaaagc catactctac taagaagatc aagttgaact tcggtatccc
     4021 aactttggct gacggttggt ctaagtctaa ggaatactct aacaacgcta tcatcttgat
     4081 gagagacaac ttgtactact tgggtatctt caacgctaag aacaagccag acaagaagat
     4141 catcgaaggt aacacttctg aaaacaaggg tgactacaag aagatgatct acaacttgtt
     4201 gccaggtcca aacaagatga tcccaaaggt tttcttgtct tctaagactg gtgttgaaac
     4261 ttacaagcca tctgcttaca tcttggaagg ttacaagcaa aacaagcaca tcaagtcttc
     4321 taaggacttc gacatcactt tctgtcacga cttgatcgac tacttcaaga actgtatcgc
     4381 tatccaccca gaatggaaga acttcggttt cgacttctct gacacttcta cttacgaaga
     4441 catctctggt ttctacagag aagttgaatt gcaaggttac aagatcgact ggacttacat
     4501 ctctgaaaag gacatcgact tgttgcaaga aaagggtcaa ttgtacttgt tccaaatcta
     4561 caacaaggac ttctctaaga agtctactgg taacgacaac ttgcacacta tgtacttgaa
     4621 gaacttgttc tctgaagaaa acttgaagga catcgttttg aagttgaacg gtgaagctga
     4681 aatcttcttc agaaagtctt ctatcaagaa cccaatcatc cacaagaagg gttctatctt
     4741 ggttaacaga acttacgaag ctgaagaaaa ggaccaattc ggtaacatcc aaatcgttag
     4801 aaagaacatc ccagaaaaca tctaccaaga attgtacaag tacttcaacg acaagtctga
     4861 caaggaattg tctgacgaag ctgctaagtt gaagaacgtt gttggtcacc acgaagctgc
     4921 tactaacatc gttaaggact acagatacac ttacgacaag tacttcttgc acatgccaat
     4981 cactatcaac ttcaaggcta acaagactgg tttcatcaac gacagaatct tgcaatacat
     5041 cgctaaggaa aaggacttgc acgttatcgg tatcgacaga ggtgaaagaa acttgatcta
     5101 cgtttctgtt atcgacactt gtggtaacat cgttgaacaa aagtctttca acatcgttaa
     5161 cggttacgac taccaaatca agttgaagca acaagaaggt gctagacaaa tcgctagaaa
     5221 ggaatggaag gaaatcggta agatcaagga aatcaaggaa ggttacttgt ctttggttat
     5281 ccacgaaatc tctaagatgg ttatcaagta caacgctatc atcgctatgg aagacttgtc
     5341 ttacggtttc aagaagggta gattcaaggt tgaaagacaa gtttaccaaa agttcgaaac
     5401 tatgttgatc aacaagttga actacttggt tttcaaggac atctctatca ctgaaaacgg
     5461 tggtttgttg aagggttacc aattgactta catcccagac aagttgaaga acgttggtca
     5521 ccaatgtggt tgtatcttct acgttccagc tgcttacact tctaagatcg acccaactac
     5581 tggtttcgtt aacatcttca agttcaagga cttgactgtt gacgctaaga gagaattcat
     5641 caagaagttc gactctatca gatacgactc tgaaaagaac ttgttctgtt tcactttcga
     5701 ctacaacaac ttcatcactc aaaacactgt tatgtctaag tcttcttggt ctgtttacac
     5761 ttacggtgtt agaatcaaga gaagattcgt taacggtaga ttctctaacg aatctgacac
     5821 tatcgacatc actaaggaca tggaaaagac tttggaaatg actgacatca actggagaga
     5881 tggtcacgac ttgagacaag acatcatcga ctacgaaatc gttcaacaca tcttcgaaat
     5941 cttcagattg actgttcaaa tgagaaactc tttgtctgaa ttggaagaca gagactacga
     6001 cagattgatc tctccagttt tgaacgaaaa caacatcttc tacgactctg ctaaggctgg
     6061 tgacgctttg ccaaaggacg ctgacgctaa cggtgcttac tgtatcgctt tgaagggttt
     6121 gtacgaaatc aagcaaatca ctgaaaactg gaaggaagac ggtaagttct ctagagacaa
     6181 gttgaagatc tctaacaagg actggttcga cttcatccaa aacaagagat acttgagcag
     6241 ggctgacccc aagaagaaga ggaaggtgta aatccttttg tataactaaa taatattgga
     6301 aactaaatac gaatacccaa attttttatc taaattttgc cgaaagatta aaatctgcag
     6361 agatatccga aacaggtaaa tggatgtttc aatccctgta gtcagtcagg aacccatatt
     6421 atattacagt attagtcgcc gcttaggcac gcctttaatt agcaaaatca aaccttaagt
     6481 gcatatgccg tataagggaa actcaaagaa ctggcatcgc aaaaatgaaa acatacgttg
     6541 aaactacggc aaaggattgg tcagatcgct tcatacaggg aaagttcggc atcaacatct
     6601 ttggataata tcagaatgag aaagaacaga tacgcagtac gttttttggt gagctctttg
     6661 cacttcttta gttctttcca tcaatatcag ttttttaaac ttttaggact aaaagtgatg
     6721 tttaacttca aaatgtttaa aattttgttc ttcccgacgt tcattaagaa tactaataca
     6781 ctttaataat tattttaata ttttgttcta aataatgact tttaattaaa aaagataaaa
     6841 tataaaaaca tcataataac tcaccagagg ttaagaacaa aaaaacaaat tagatatctg
     6901 ctaatccaat atagttaaat caatctttcc ttggtataat gggtatatta catatatttc
     6961 aaggaccgac actcctacca aatatctaaa atttaccata ttaacataac atgtatataa
     7021 acgtcaaatc ataatcagca ctaacgtctc acggatcgta tatgccgtag cgacaatcta
     7081 agaactatgc gaggacacgc tagggccaag gcaaaaagat tccttgatta cgtaagggag
     7141 ttagaatcat tttgaataaa aaacacgctt tttcagttcg agtttatcat tatcaatact
     7201 gccatttcaa agaatacgta aataattaat agtagtgatt ttcctaactt tatttagtca
     7261 aaaaattagc cttttaattc tgctgtaacc cgtacatgcc caaaataggg ggcgggttac
     7321 acagaatata taacatcgta ggtgtctggg tgaacagttt attcctggca tccactaaat
     7381 ataatggagc ccgcttttta agctggcatc cagaaaaaaa aagaatccca gcaccaaaat
     7441 attgttttct tcaccaacca tcagttcata ggtccattct cttagcgcaa ctacagagaa
     7501 caggggcaca aacaggcaaa aaacgggcac aacctcaatg gagtgatgca acctgcctgg
     7561 agtaaatgat gacacaaggc aattgaccca cgcatgtatc tatctcattt tcttacacct
     7621 tctattacct tctgctctct ctgatttgga aaaagctgaa aaaaaaggtt gaaaccagtt
     7681 ccctgaaatt attcccctac ttgactaata agtatataaa gacggtaggt attgattgta
     7741 attctgtaaa tctatttctt aaacttctta aattctactt ttatagttag tctttttttt
     7801 agttttaaaa caccaagaac ttagtttcga ataaacacac ataaacaaac aaacgaaatt
     7861 ctgatgagtc cgtgaggacg aaacgagcta gctcgtcaat ttctactctt gtagatagag
     7921 accgaaagtg aaacgtgatt tcatgcgtca ttttgaacat tttgtaaatc ttatttaata
     7981 atgtgtgcgg caattcacat ttaatttatg aatgttttct taacatcgcg gcaactcaag
     8041 aaacggcagg ttcggatctt agctactaga gaaagaggag aaatactaga tgcgtaaagg
     8101 cgaagagctg ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg
     8161 tcataagttt tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct
     8221 gaagttcatc tgtactactg gtaaactgcc ggttccttgg ccgactctgg taacgacgct
     8281 gacttatggt gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt
     8341 caagtccgcc atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg
     8401 cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga
     8461 gctgaaaggc attgacttta aagaggacgg caatatcctg ggccataagc tggaatacaa
     8521 ttttaacagc cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa
     8581 ttttaaaatt cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca
     8641 aaacactcca atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca
     8701 aagcgttctg tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt
     8761 aaccgcagcg ggcatcacgc atggtatgga tgaactgtac aaatgaccag gcatcaaata
     8821 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     8881 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttataggtct
     8941 caaatttcta ctcttgtaga tggccggcat ggtcccagcc tcctcgctgg cgccggctgg
     9001 gcaacatgct tcggcatggc gaatgggacg tgaatttact ttaaatcttg catttaaata
     9061 aattttcttt ttatagcttt atgacttagt ttcaatttat atactatttt aatgacattt
     9121 tcgattcatt gattgaaagc tttgtgtttt ttcttgatgc gctattgcat tgttcttgtc
     9181 tttttcgcca catgtaatat ctgtagtaga tacctgatac attgtggatg ctgagtgaaa
     9241 ttttagttaa taatggaggc gctcttaata attttgggga tattggcttt tttttttaaa
     9301 gtttacaaat gaattttttc cgccaggata acgattctga agttactatt cacgtagacg
     9361 gataggtata gccagacatc agcagcatac ttcgggaacc gtaggccacc gagcggccgc
     9421 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9481 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9541 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9601 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc
     9661 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9721 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9781 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9841 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9901 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9961 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10021 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10081 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10141 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10201 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10261 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10321 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10381 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10441 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10501 cacctgtcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    10741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    10921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    11041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt gcgg
//
